BBa_K562013
1
PduD40-GFP
Salty_PduD40-GFP
2011-09-16T11:00:00Z
2015-05-08T01:12:41Z
Derived from Salmonella enterica serovar Typhimurium LT2 genomic sequence and an unknown cloning vector encoding GFP.
This is a composite part comprising a constitutive promoter, which is the tatABCD promoter from E. coli K-12, driving production of the initial 40 residues of the PduD protein from Salmonella enterica serovar Typhimurium LT2 (identical to part BBa_K562002), which is itself fused in-frame to GFP. The GFP gene product also carries a C-terminal HA epitope tag. Production of PduD40-FP has been verified by Western immunoblotting (anti-GFP). The construct is cloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-D40-GFP in the Sargent Laboratory, Dundee, UK.
false
false
_730_
0
8083
9
It's complicated
false
None
false
Frank Sargent
annotation2131002
1
PduD40 Tag
range2131002
1
119
238
annotation2131000
1
BBa_K562002
range2131000
1
1
244
annotation2131004
1
HA epitope tag
range2131004
1
956
982
annotation2131001
1
synthetic RBS
range2131001
1
107
112
annotation2131003
1
GFP
range2131003
1
245
955
BBa_K562013_sequence
1
tgtcggttggcgcaaaacacgctgattttttcatcgctcaaggcgggccgtgtaacgtataatgcggctttgtttaatcatcatctaccacagaggaggatcacacagaggaacaggtatggaaattaatgaaaaattgctgcgccagataattgaagacgtactccgcgatatgaagggcagcgataaacccgtctcgtttaatgcgcctgcggcatccacagcaccacagaccgcttctagaagtaaaggagaagaacttttcactggagtggtcccagttcttgttgaattagatggcgatgttaatgggcaaaaattctctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaattttatttgcactactgggaagctacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttctcaagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatattttacaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaaatggaatacaactataactcacataatgtatacatcatgggagacaaaccaaagaatggcatcaaagttaacttcaaaattagacacaacattaaagatggaagcgttcaattagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttccaaagatcccaacgaaaagagagatcacatgatccttcttgagtttgtaacagctgctaggattacacatggcatggatgaactatacaaatatccgtacgatgtgccggactatgcgtaaggatcc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z