BBa_K562014
1
PduD40-Bfr
Salty_PduD40-Bfr
2011-09-16T11:00:00Z
2015-05-08T01:12:41Z
Derived from Salmonella enterica serovar Typhimurium LT2 and Escherichia coli K-12 MG1655 genomic sequences.
This is a composite part comprising a constitutive promoter, which is the tatABCD promoter from E. coli K-12, driving production of the initial 40 residues of the PduD protein from Salmonella enterica serovar Typhimurium LT2 (identical to part BBa_K562002), which is itself fused in-frame to bacterio-ferritin (Bfr) from Escherichia coli K-12. The Bfr gene product also carries a C-terminal HA epitope tag. The construct is cloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-D40-Bfr in the Sargent Laboratory, Dundee, UK.
false
false
_730_
0
8083
9
It's complicated
false
None
false
Frank Sargent
annotation2131010
1
synthetic RBS
range2131010
1
107
112
annotation2131012
1
bacterioferritin
range2131012
1
245
715
annotation2131013
1
HA epitope tag
range2131013
1
716
742
annotation2131009
1
BBa_K562002
range2131009
1
1
244
annotation2131011
1
PduD40 Tag
range2131011
1
119
238
BBa_K562014_sequence
1
tgtcggttggcgcaaaacacgctgattttttcatcgctcaaggcgggccgtgtaacgtataatgcggctttgtttaatcatcatctaccacagaggaggatcacacagaggaacaggtatggaaattaatgaaaaattgctgcgccagataattgaagacgtactccgcgatatgaagggcagcgataaacccgtctcgtttaatgcgcctgcggcatccacagcaccacagaccgcttctagaaaaggtgatactaaagttataaattatctcaacaaactgttgggaaatgagcttgtcgcaatcaatcagtactttctccatgcccgaatgtttaaaaactggggtctcaaacgtctcaatgatgtggagtatcatgaatccattgatgagatgaaacacgccgatcgttatattgagcgcattctttttctggaaggtcttccaaacttacaggacctgggcaaactgaacattggtgaagatgttgaggaaatgctgcgttctgatctggcacttgagctggatggcgcgaagaatttgcgtgaggcaattggttatgccgatagcgttcatgattacgtcagccgcgatatgatgatagaaattttgcgtgatgaagaaggccatatcgactggctggaaacggaacttgatctgattcagaagatgggcctgcaaaattatctgcaagcacagatccgcgaagaaggttatccgtacgatgtgccggactatgcgtaaggatcc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z