BBa_K562014 1 PduD40-Bfr Salty_PduD40-Bfr 2011-09-16T11:00:00Z 2015-05-08T01:12:41Z Derived from Salmonella enterica serovar Typhimurium LT2 and Escherichia coli K-12 MG1655 genomic sequences. This is a composite part comprising a constitutive promoter, which is the tatABCD promoter from E. coli K-12, driving production of the initial 40 residues of the PduD protein from Salmonella enterica serovar Typhimurium LT2 (identical to part BBa_K562002), which is itself fused in-frame to bacterio-ferritin (Bfr) from Escherichia coli K-12. The Bfr gene product also carries a C-terminal HA epitope tag. The construct is cloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-D40-Bfr in the Sargent Laboratory, Dundee, UK. false false _730_ 0 8083 9 It's complicated false None false Frank Sargent annotation2131010 1 synthetic RBS range2131010 1 107 112 annotation2131012 1 bacterioferritin range2131012 1 245 715 annotation2131013 1 HA epitope tag range2131013 1 716 742 annotation2131009 1 BBa_K562002 range2131009 1 1 244 annotation2131011 1 PduD40 Tag range2131011 1 119 238 BBa_K562014_sequence 1 tgtcggttggcgcaaaacacgctgattttttcatcgctcaaggcgggccgtgtaacgtataatgcggctttgtttaatcatcatctaccacagaggaggatcacacagaggaacaggtatggaaattaatgaaaaattgctgcgccagataattgaagacgtactccgcgatatgaagggcagcgataaacccgtctcgtttaatgcgcctgcggcatccacagcaccacagaccgcttctagaaaaggtgatactaaagttataaattatctcaacaaactgttgggaaatgagcttgtcgcaatcaatcagtactttctccatgcccgaatgtttaaaaactggggtctcaaacgtctcaatgatgtggagtatcatgaatccattgatgagatgaaacacgccgatcgttatattgagcgcattctttttctggaaggtcttccaaacttacaggacctgggcaaactgaacattggtgaagatgttgaggaaatgctgcgttctgatctggcacttgagctggatggcgcgaagaatttgcgtgaggcaattggttatgccgatagcgttcatgattacgtcagccgcgatatgatgatagaaattttgcgtgatgaagaaggccatatcgactggctggaaacggaacttgatctgattcagaagatgggcctgcaaaattatctgcaagcacagatccgcgaagaaggttatccgtacgatgtgccggactatgcgtaaggatcc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z