BBa_K562016 1 PduD40-Xyl Salty_PduD40-XylA 2011-09-17T11:00:00Z 2015-05-08T01:12:41Z Derived from Salmonella enterica serovar Typhimurium LT2 and Escherichia coli K-12 MG1655 genomic sequences. This is a composite part comprising a constitutive promoter, which is the tatABCD promoter from E. coli K-12, driving production of the initial 40 residues of the PduD protein from Salmonella enterica serovar Typhimurium LT2 (identical to part BBa_K562002), which is itself fused in-frame to D-xylose isomerase (XylA) from Escherichia coli K-12. The XylA gene product also carries a C-terminal HA epitope tag. Production of PduD20-XylA has been verified by Western immunoblotting (anti-HA). The construct is cloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-D40-XylA in the Sargent Laboratory, Dundee, UK. false false _730_ 0 8083 9 It's complicated false None false Frank Sargent annotation2131635 1 synthetic RBS range2131635 1 107 112 annotation2131638 1 HA epitope tag range2131638 1 1587 1613 annotation2131636 1 PduD40 Tag range2131636 1 119 238 annotation2131637 1 XylA range2131637 1 245 1586 annotation2131634 1 BBa_K562002 range2131634 1 1 244 BBa_K562016_sequence 1 tgtcggttggcgcaaaacacgctgattttttcatcgctcaaggcgggccgtgtaacgtataatgcggctttgtttaatcatcatctaccacagaggaggatcacacagaggaacaggtatggaaattaatgaaaaattgctgcgccagataattgaagacgtactccgcgatatgaagggcagcgataaacccgtctcgtttaatgcgcctgcggcatccacagcaccacagaccgcttctagacaagcctattttgaccagctcgatcgcgttcgttatgaaggctcaaaatcctcaaacccgttagcattccgtcactacaatcccgacgaactggtgttgggtaagcgtatggaagagcacttgcgttttgccgcctgctactggcacaccttctgctggaacggggcggatatgtttggtgtgggggcgtttaatcgtccgtggcagcagcctggtgaggcactggcgttggcgaagcgtaaagcagatgtcgcatttgagtttttccacaagttacatgtgccattttattgcttccacgatgtggatgtttcccctgagggcgcgtcgttaaaagagtacatcaataattttgcgcaaatggttgatgtcctggcaggcaagcaagaagagagcggcgtgaagctgctgtggggaacggccaactgctttacaaaccctcgctacggcgcgggtgcggcgacgaacccagatcctgaagtcttcagctgggcggcaacgcaagttgttacagcgatggaagcaacccataaattgggcggtgaaaactatgtcctgtggggcggtcgtgaaggttacgaaacgctgttaaataccgacttgcgtcaggagcgtgaacaactgggccgctttatgcagatggtggttgagcataaacataaaatcggtttccagggcacgttgcttatcgaaccgaaaccgcaagaaccgaccaaacatcaatatgattacgatgccgcgacggtctatggcttcctgaaacagtttggtctggaaaaagagattaaactgaacattgaagctaaccacgcgacgctggcaggtcactctttccatcatgaaatagccaccgccattgcgcttggcctgttcggttctgtcgacgccaaccgtggcgatgcgcaactgggctgggacaccgaccagttcccgaacagtgtggaagagaatgcgctggtgatgtatgaaattctcaaagcaggcggtttcaccaccggtggtctgaacttcgatgccaaagtacgtcgtcaaagtactgataaatatgatctgttttacggtcatatcggcgcgatggatacgatggcactggcgctgaaaattgcagcgcgcatgattgaagatggcgagctggataaacgcatcgcgcagcgttattccggctggaatagcgaattgggccagcaaatcctgaaaggccaaatgtcactggcagatttagccaaatatgctcaggaacatcatttgtctccggtgcatcagagtggtcgccaggaacaactggaaaatctggtaaaccattatctgttcgacaaaggtaaaccattatctgttcgacaaatatccgtacgatgtgccggactatgcgtaaggatcc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z