BBa_K562019 1 PduD40-GFP Salty_PduD40-GFPssrA 2011-09-17T11:00:00Z 2015-05-08T01:12:42Z Derived from Salmonella enterica serovar Typhimurium LT2 genomic sequence and a plasmid described in this paper: DeLisa MP, Samuelson P, Palmer T, Georgiou G. Genetic analysis of the twin arginine translocator secretion pathway in bacteria. J Biol Chem. 2002 Aug 16;277(33):29825-31. This is a composite part comprising a constitutive promoter, which is the tatABCD promoter from E. coli K-12, driving production of the initial 40 residues of the PduD protein from Salmonella enterica serovar Typhimurium LT2 (identical to part BBa_K562002), which is itself fused in-frame to GFP. The GFP gene product also carries a C-terminal ssrA degradation tag. Production of PduD40-GFP-ssrA has been verified by Western immunoblotting (anti-GFP) when co-expressed with BBa_K562009 encosing a bacterial microcompartment (BMC). The construct is cloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-D40-GFPssrA in the Sargent Laboratory, Dundee, UK. false false _730_ 0 8083 9 It's complicated false None false Frank Sargent annotation2131873 1 PduD40 Tag range2131873 1 119 238 annotation2131871 1 BBa_K562002 range2131871 1 1 244 annotation2131874 1 GFP range2131874 1 245 955 annotation2131875 1 ssrA degradation tag range2131875 1 962 994 annotation2131872 1 synthetic RBS range2131872 1 107 112 BBa_K562019_sequence 1 tgtcggttggcgcaaaacacgctgattttttcatcgctcaaggcgggccgtgtaacgtataatgcggctttgtttaatcatcatctaccacagaggaggatcacacagaggaacaggtatggaaattaatgaaaaattgctgcgccagataattgaagacgtactccgcgatatgaagggcagcgataaacccgtctcgtttaatgcgcctgcggcatccacagcaccacagaccgcttctagaagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcgcgtatggtcttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaagtcgacgcagcaaacgacgaaaactacgctttagcagcttaagcatgcaagctt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z