BBa_K563002 1 BBa_K563002 promoter of ILV2 2011-10-04T11:00:00Z 2015-05-08T01:12:42Z Amplified from the genome of S.cerevisiae by PCR Promoter of ILV2, under control of transcription factor Gcn4. In our project, when When TORC1 is inhibited by FAP, dephosphorylated Gcn4 would bind with pHIS4 and activate downstream genes. We put the target gene of mutant Tor2 under this promoter, and then its expression could be controlled by the amount of FAP and the activity of TORC1. Mutant Tor2 would be much resistant to FAP and a feedback loop is formed between FAP, TORC1, Gcn4 and mTor. false false _731_ 0 7195 9 It's complicated false Design NcoI and XhoI at two ends of the promoter false Boxuan Zeng annotation2154710 1 promoter of ILV2 range2154710 1 1 509 BBa_K563002_sequence 1 gcacggatgaaaggtgacaaacgcctagccgccggagcctgccggtaccggcttggcttcagttgctgatctcggcgcggaaaaatcagcgccccacgccaaaaggttcgtattttttctttttttttctaatcttccatctattcggtagcgatgattcatttctctgaaaaaaaaaaaaaaaaaaaaaaatgaaaaagaatatttttttgatgaacttgtatttctcttatctggttgatatatatgctatcatttattttcttatcaagtttccaaatttctaatcctttctccaccatccctaattaataattcagatctacgtcacaccgtaatttgtattgtttttttccttcattgtctaaaaccgaagaattcatcagccacagttactagttcatttgaagcgaaattacacacattttccctgttacaatagaaagtattttacaaaatctaaaccctttgagctaagaggagataaatacaacagaatcaattttcaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z