LOCUS BBa_K563030 6996 bp DNA linear 23-Dec-2024 DEFINITION pEASY with first part of Tor2 ACCESSION BBa_K563030 VERSION BBa_K563030.1 FEATURES Location/Qualifiers CDS 294..3360 /label=01Tor2 promoter 3430..3447 /label=T7 promoter CDS 4383..5177 /label=Kanamycin resistance ORF CDS 5195..6055 /label=Amplicillin ORIGIN 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg 241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattgcc cttcacagca 301 attaaaagtc ggagaacctg actgaaaatt catgaatctc ttcatttcta tagcctttcc 361 tctatgcatt tgtattatat atttattacc gtcatttttt acatactgct gcattttggc 421 gccagtgata agtggcaaac aattcgacgg aatcgtggta attataccac gttactctat 481 aacatcatga tattgcaatt aatcaaacat acatttaatc ttaatgctat tagcttacta 541 caactctttt ctttaagtta tatcgtatat ttcttgggcg atgtcagaat atttacccgg 601 atattccttt ttaagcactg aatatgtttg aatagagact gacatatatg gcagcaatta 661 aaattggaag aaatgtaatg acagtaggaa agaccaattt ttatcatcgt gacaccaatc 721 acttccttaa ctgagcttta cttgtattta tttacaggta gattaggagc agtagaaagg 781 gaaaatatac cgggtgcata aagagcatag tcattaagat caaatagtta tctttctcaa 841 agagatttct gatctttact ttccccatat gaaaaatgaa taaatacatt aacaaataca 901 ccacgccacc taacttattg tctttacgac aaagggccga aggcaaacac agaacaagaa 961 agaaacttac acacaaatcg cactcccacg atgatgagat gtcaactact tcaaacacag 1021 attccaatca caatgggccc aatgactctg gtagagtgat cactggttct gctggtcata 1081 ttggtaaaat atcctttgta gattcagaac tagatacaac attttctact ttaaatttga 1141 tttttgataa acttaaaagc gatgtgccac aagaacgagc ctctggcgct aatgaattaa 1201 gcactacttt gacctcatta gcaagggaag tatctgctga gcaatttcaa aggtttagca 1261 acagtttaaa caataagata tttgaactta ttcacgggtt tacttcaagt gagaagatag 1321 gtggtattct tgctgttgat actctgatct cattctacct gagtacagag gagctgccaa 1381 accaaacttc aagactggcg aactatttac gtgttttaat tccatccagt gacattgaag 1441 ttatgagatt agcggctaac accttaggta gattgaccgt gccaggtggt acattaacat 1501 cagatttcgt cgaatttgag gtcagaactt gcattgattg gcttactctg acagcagata 1561 ataactcatc gagctctaag ttggaataca ggagacatgc tgcgctatta atcataaagg 1621 cattagcaga caattcaccc tatcttttat acccttacgt taactctatc ttagacaata 1681 tttgggtgcc attaagggat gcaaagttaa ttatacgatt agatgccgca gtggcattgg 1741 gtaaatgtct tactattatt caggatagag accctgcttt gggaaaacag tggtttcaaa 1801 gattatttca aggttgtaca catggcttaa gtctcaatac gaatgattca gtgcatgcta 1861 ctctgttggt atttcgagaa ttactcagct tgaaagcacc ttatctcagg gataaatatg 1921 atgatattta caaatctact atgaagtaca aggaatataa atttgatgtt ataaggagag 1981 aagtttatgc tattttacct cttttagctg cttttgaccc tgccattttc acaaagaaat 2041 atctcgatag gataatggtt cattatttaa gatatttgaa gaacatcgat atgaatgctg 2101 caaataattc ggataaacct tttatattag tttctatagg tgatattgca tttgaagttg 2161 gttcgagcat ttcaccctat atgacactta ttctggataa tattagggaa ggcttaagaa 2221 cgaaattcaa agttagaaaa caattcgaga aggatttatt ttattgcatt ggtaaattag 2281 cttgtgcttt gggcccagct tttgctaagc acttgaacaa agatcttctt aatttgatgt 2341 taaactgtcc aatgtccgac catatgcagg agactttaat gatccttaac gagaaaatac 2401 cctctttgga atctaccgtt aattcgagga tactaaattt actgtcgata tccttatctg 2461 gtgaaaaatt tattcaatca aaccaatacg attttaataa tcaattttcc attgaaaagg 2521 ctcgtaaatc aagaaaccaa agtttcatga aaaaaactgg tgaatctaat gacgatatta 2581 cagatgccca aattttgatt cagtgtttta aaatgctgca actaattcat catcaatatt 2641 ccttgacgga gtttgttagg cttataacca tttcttacat tgagcatgag gattcgtctg 2701 tcagaaaatt ggcagcatta acgtcgtgtg atttatttat caaagacgat atatgtaaac 2761 aaacatcagt tcatgcttta cactcggttt ctgaagtgct aagtaagcta ttaatgatcg 2821 caataactga tccggttgca gaaattagat tggaaattct tcagcatttg gggtcaaatt 2881 ttgatcctca attggcccaa ccagacaatt tacgcctact tttcatggcg ctgaacgatg 2941 agatttttgg tattcaattg gaagctatca aaataatagg cagattgagt tctgtcaacc 3001 ccgcttatgt agttccttct ttgaggaaaa ctttactgga actattaacg caattgaagt 3061 tctcaaatat gccaaaaaaa aaggaggaaa gtgcaactct attatgtacg ctgataaatt 3121 ccagcgatga agtagcgaaa ccttatattg atcctattct agacgtcatt cttcctaaat 3181 gccaggatgc ttcatctgcc gtagcatcca ccgctttaaa ggttttgggt gaactatctg 3241 ttgttggagg aaaagaaatg acgcgttact taaaggaatt gatgccattg atcattaaca 3301 catttcagga ccaatcaaac tcttttaaaa gagatgccgc cttaacaaca ttaggacagc 3361 aagggcaatt ctgcagatat ccatcacact ggcggccgct cgagcatgca tctagagggc 3421 ccaattcgcc ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg 3481 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca 3541 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga 3601 atggcgaatg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 3661 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 3721 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 3781 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 3841 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 3901 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 3961 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 4021 acaaaaattt aacgcgaatt ttaacaaaat tcagggcgca agggctgcta aaggaagcgg 4081 aacacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt cagctactgg 4141 gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg cagtgggctt 4201 acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga attgccagct 4261 ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc tttcttgccg 4321 ccaaggatct gatggcgcag gggatcaaga tctgatcaag agacaggatg aggatcgttt 4381 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta 4441 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg 4501 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa 4561 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct 4621 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg 4681 caggatctcc tgtcatccca ccttgctcct gccgagaaag tatccatcat ggctgatgca 4741 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat 4801 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac 4861 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc 4921 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa 4981 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag 5041 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc 5101 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt 5161 cttgacgagt tcttctgaat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 5221 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 5281 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 5341 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 5401 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac 5461 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 5521 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 5581 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 5641 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 5701 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 5761 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 5821 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 5881 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc 5941 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 6001 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 6061 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 6121 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 6181 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 6241 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 6301 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 6361 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 6421 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 6481 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 6541 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 6601 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 6661 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 6721 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 6781 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 6841 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 6901 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga 6961 ccgagcgcag cgagtcagtg agcgaggaag cggaag //