LOCUS BBa_K563034 6638 bp DNA linear 06-Dec-2025 DEFINITION pEASY+22Tor2(with L->P mutation) ACCESSION BBa_K563034 VERSION BBa_K563034.1 FEATURES Location/Qualifiers CDS 294..3002 /label=22Tor2(LP mutation) CDS 4838..5698 /label=Ampicillin Resistance ORF CDS 4026..4820 /label=Kanamycin Resistance ORF misc_feature 2173..2175 /label=LP mutation ORIGIN 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg 241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattgcc ctttgttgtt 301 ggaggaaaag aaatgacgcg ttacttaaag gaattgatgc cattgatcat taacacattt 361 caggaccaat caaactcttt taaaagagat gccgccttaa caacattagg acagctggct 421 gcttcctctg gttatgttgt tggcccttta ctagactacc cagagttact tggcattttg 481 ataaatattc ttaagactga aaacaaccct catatcaggc gtggaactgt tcgtttgatt 541 ggtatattag gcgctcttga tccatataag cacagagaaa tagaagtcac atcaaactca 601 aagagttcag tagagcaaaa tgctccttca atcgacatcg cattgctaat gcaaggggta 661 tctccatcca acgatgaata ttaccccact gtagttatcc acaatctgat gaagatattg 721 aatgatccat cgttgtcaat ccatcacacg gctgctattc aagctattat gcatattttt 781 caaaaccttg gtttacgatg tgtctccttt ttggatcaaa ttattccagg tatcatttta 841 gtcatgcgtt catgcccgcc gtcccaactt gacttttatt ttcagcaact gggatctctc 901 atctcaattg tcaagcaaca tattaggccc catgtcgaga aaatttatgg tgtgatcagg 961 gagtttttcc cgatcattaa actacaaatc acaattattt ctgtcataga atcgatatct 1021 aaggctctgg aaggtgagtt taaaagattt gttcccgaga ctctaacctt tttccttgat 1081 attcttgaga acgaccagtc taataaaagg atcgttccga ttcgtatatt aaaatctttg 1141 gttacttttg ggccgaatct agaagactat tcccatttga ttatgcctat cgttgttaga 1201 atgactgagt attctgctgg aagtctaaag aaaatctcca ttataacttt gggtagatta 1261 gcaaagaata tcaacctctc tgaaatgtca tcaagaattg ttcaggcgtt ggtaagaatt 1321 ttgaataatg gggatagaga actaacaaaa gcaaccatga atacgctaag tttgctcctt 1381 ttacaactag gtaccgactt tgtggtcttt gtgccagtga ttaacaaggc gttattgagg 1441 aataggattc agcattcagt gtacgatcaa ctggttaata aattactgaa caatgaatgc 1501 ttgccaacaa atatcatatt tgacaaggag aacgaagtac ctgaaaggaa aaattatgaa 1561 gacgaaatgc aagtaacgaa attaccggta aaccaaaata tcctaaagaa tgcatggtat 1621 tgttctcaac agaagaccaa agaagattgg caagaatgga taagaaggct atctattcag 1681 cttctaaagg aatcaccttc agcttgtcta cgatcctgtt cgagtttagt cagcgtttat 1741 tatccgttgg cgagagaatt gtttaatgct tcattctcaa gttgctgggt tgagcttcaa 1801 acgtcatacc aagaggattt gattcaagca ttatgcaagg ctttatcatc ctctgaaaac 1861 ccacccgaga tttatcaaat gttgttaaat ttagtggaat ttatggagca cgatgacaaa 1921 ccattgccta tcccaatcca tacattaggt aagtatgccc aaaaatgtca tgcttttgcg 1981 aaggcactac attacaaaga ggtagaattc ttagaagagc cgaaaaattc aacaatcgag 2041 gcattgatta gcattaataa tcaacttcac caaactgatt ctgctattgg tattttgaag 2101 catgcgcaac aacacaatga attgcagctg aaggaaactt ggtatgaaaa acttcaacgt 2161 tgggaggatg ctccagcagc atataatgag aaggaggcag caggagaaga ttcggttgaa 2221 gtgatgatgg gaaaattaag atcgttatat gcccttggag agtgggaaga gctttctaaa 2281 ttggcatctg aaaagtgggg cacggcaaaa cccgaagtga agaaggcaat ggcgcctttg 2341 gctgccggcg ctgcctgggg tttggagcaa tgggatgaaa tagcccagta tactagcgtc 2401 atgaaatcgc agtctccaga taaagaattc tatgatgcaa ttttatgttt gcataggaat 2461 aattttaaga aggcggaagt tcacatcttt aatgcaaggg atcttctagt tactgaattg 2521 tcagctcttg ttaatgaaag ctacaataga gcatataatg ttgttgttag agcgcagatt 2581 atagcagagt tggaggaaat catcaaatat aagaagttgc cacaaaattc agataaacgt 2641 ctaactatga gagaaacttg gaataccaga ttactgggct gtcaaaaaaa tattgatgtg 2701 tggcaaagaa ttctgcgtgt cagatcattg gtgataaagc caaaggagga tgctcaagtg 2761 aggattaagt ttgccaactt atgcagaaaa tcgggtagga tggcgctagc taaaaaagtc 2821 ttaaatacat tgcttgaaga aacagatgac ccagatcatc ctaatactgc taaggcatcc 2881 cctccagttg tttatgcaca actgaagtac ttgtgggcta cggggttgca agatgaggct 2941 ttgaagcaat taattaattt cacatctaga atggctcatg atttaggttt ggatccaaat 3001 aaaagggcaa ttctgcagat atccatcaca ctggcggccg ctcgagcatg catctagagg 3061 gcccaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg 3121 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc 3181 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct 3241 gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg 3301 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct 3361 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta 3421 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt 3481 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg 3541 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat 3601 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt 3661 taacaaaaat ttaacgcgaa ttttaacaaa attcagggcg caagggctgc taaaggaagc 3721 ggaacacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat gtcagctact 3781 gggctatctg gacaagggaa aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc 3841 ttacatggcg atagctagac tgggcggttt tatggacagc aagcgaaccg gaattgccag 3901 ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc 3961 cgccaaggat ctgatggcgc aggggatcaa gatctgatca agagacagga tgaggatcgt 4021 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc 4081 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc 4141 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg 4201 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag 4261 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg 4321 ggcaggatct cctgtcatcc caccttgctc ctgccgagaa agtatccatc atggctgatg 4381 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac 4441 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg 4501 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc 4561 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg 4621 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc 4681 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc 4741 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc 4801 ttcttgacga gttcttctga attgaaaaag gaagagtatg agtattcaac atttccgtgt 4861 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct 4921 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga 4981 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag 5041 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca 5101 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga 5161 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag 5221 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc 5281 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa 5341 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt 5401 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg 5461 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt 5521 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg 5581 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat 5641 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact 5701 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa 5761 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 5821 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 5881 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 5941 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 6001 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt 6061 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 6121 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 6181 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 6241 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 6301 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 6361 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 6421 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 6481 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga 6541 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac 6601 gaccgagcgc agcgagtcag tgagcgagga agcggaag //