BBa_K563036 1 BBa_K563036 pEASY+33Tor2(with V->A mutation) 2011-10-04T11:00:00Z 2015-05-08T01:12:42Z pEASY vector is bought from Transgene Company, and we get mutant-33Tor2 by mutating the 33Tor2 from genomic sequence. There are totally four mutation sites as we designed in the complete Tor2 sequence. This BioBrick contains the third part of Tor2 with mutation that from amino acid V to amino acid A. false false _731_ 0 10236 9 It's complicated false We will conduct this mutation by designing primers with mutant site. After we get this mutant part of Tor2, we can keep it for further connection. false Guo Jing annotation2154701 1 Ampicillin resistance ORF range2154701 1 4628 5488 annotation2154706 1 Kanamycin resistance ORF range2154706 1 3816 4610 annotation2154720 1 VA mutation site range2154720 1 1672 1674 annotation2154705 1 33Tor2 (VA mutation) range2154705 1 294 2792 BBa_K563036_sequence 1 agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgccaagcttggtaccgagctcggatccactagtaacggccgccagtgtgctggaattgcccttagaatggctcatgatttaggtttggatccaaataatatgatagctcaaagcgttcctcaacaaagcaaaagagtccctcgtcacgttgaagattatactaagcttttagctcgttgtttcttgaagcaaggagaatggagagtttgcttacagcctaaatggagattgagcaatccagattcgatcctaggctcctatttgctcgctacacattttgacaacacatggtacaaagcgtggcataactgggcactggccaattttgaagtcatttctatgctaacatctgtctctaaaaagaaacaggaaggaagtgatgcttcctcggtaactgatattaatgagtttgataatggcatgatcggcgtcaatacatttgatgctaaggaagttcattactcttctaatttaatacacaggcacgtaattccagcaattaagggtttttttcattccatttctttatcagaatcaagctctcttcaagatgcattaaggttattaactttatggtttacttttggtggtattccagaagcaacccaagctatgcacgagggtttcaacctaatccaaataggcacatggttagaagtgttgccacagttaatttctagaattcatcaacccaatcaaattgttagtaggtcattactctccctattatctgatctaggtaaggctcatccgcaggcattagtgtaccccttaatggttgcgattaaatccgaatctctctcacgacagaaagcagctttgtccatcatagaaaagatgagaatacatagtccagttttggtcgaccaggctgaacttgtcagccacgaattgatacgtatggcggtgctttggcatgagcaatggtatgagggtctggatgacgccagtaggcagttttttggagaacataataccgaaaaaatgtttgctgctttagagcctctgtacgaaatgctgaagagaggaccggaaactttgagggaaatatcgttccaaaattcttttggtagggacttgaatgacgcttacgaatggctgatgaattacaaaaaatctaaagatgttagtaatttaaaccaagcgtgggacatttactataatgttttcaggaaaattggtaaacagttgccacaattacaaactcttgaactacaacatgtgtcgccaaaactactatctgcgcatgatttggaattggctgtccccgggacccgtgcaagtggtggaaaaccaattgttaaaatatctaaattcgagccagtattttcagtaatctcatccaaacaaagaccgagaaagttttgtatcaagggtagtgatggtaaagattataagtatgtgttgaaaggacatgaagacattagacaggatagcttggctatgcaattattcggactagttaacacgcttttgcaaaatgacgctgagtgctttagaaggcatctagatatccagcaatatccagcaatcccattatctccgaagtctgggttactgggttgggtaccgaatagtgacacgttccatgtattaattagggagcatagagaagccaaaaaaattcctttaaacattgagcattgggtcatgttacaaatggcacctgattatgacaatttaacgttgttgcagaaagtagaagtcttcacttacgccctaaataatacggagggacaagatctttataaggtgttatggctgaagagtaggtcatcggaaacgtggttggagcgtagaactacttacactcgatcgctagccgtgatgtccatgaccggttatatattggggttaggtgaccgccaccctagtaatttgatgttggatagaatcactgggaaagtcattcatattgattttggtgattgtttcgaggctgctatattaagagaaaaattccccgaaaaagtaccttttagattaactagaatgttaacatatgcaatggaagtgagtggaattgaaggtagcttccgtattacttgtgagaatgttatgaaggtacttagagataacaagggttcattaatggcaatccttgaagcttttgctttcgatcctttgatcaattggggttttgacttaccaacaaagaaaattgaggaagaaacgggcattcaacttcccgtgatgaatgccaatgagctattgagtaatggggctattaccgaagaagaagttcaaagggtggaaaacgagcacaagaatgccattcgaaatgcaagggccatgttggtattgaagcgcattactgacaaattaacggggaacgatataagaaggtttaatgacttggacgttccagaacaagtggataaactaatccaacaagccacatcagtggaaaacctatgccaacattatatcggttggtgtccattctggtagttttaatgtattgaaaatcacttagttgtatatagcctgtaaaagtgttgtataaaaatgcatctcattcattaatgtttagttccgagcgtgacgttactaactgcgaaaaagggcaattctgcagatatccatcacactggcggccgctcgagcatgcatctagagggcccaattcgccctatagtgagtcgtattacaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaattcagggcgcaagggctgctaaaggaagcggaacacgtagaaagccagtccgcagaaacggtgctgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcggttttatggacagcaagcgaaccggaattgccagctggggcgccctctggtaaggttgggaagccctgcaaagtaaactggatggctttcttgccgccaaggatctgatggcgcaggggatcaagatctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatcccaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgcgcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z