LOCUS BBa_K563037 6428 bp DNA linear 22-Nov-2024 DEFINITION pEASY+33Tor2(with E->K mutation) ACCESSION BBa_K563037 VERSION BBa_K563037.1 FEATURES Location/Qualifiers CDS 294..2792 /label=33Tor2 (EK mutation) CDS 3816..4610 /label=Kanamycin resistance ORF CDS 4628..5488 /label=Ampicillin Resistance ORF misc_feature 2333..2335 /label=EK mutation site ORIGIN 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg 241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattgcc cttagaatgg 301 ctcatgattt aggtttggat ccaaataata tgatagctca aagcgttcct caacaaagca 361 aaagagtccc tcgtcacgtt gaagattata ctaagctttt agctcgttgt ttcttgaagc 421 aaggagaatg gagagtttgc ttacagccta aatggagatt gagcaatcca gattcgatcc 481 taggctccta tttgctcgct acacattttg acaacacatg gtacaaagcg tggcataact 541 gggcactggc caattttgaa gtcatttcta tgctaacatc tgtctctaaa aagaaacagg 601 aaggaagtga tgcttcctcg gtaactgata ttaatgagtt tgataatggc atgatcggcg 661 tcaatacatt tgatgctaag gaagttcatt actcttctaa tttaatacac aggcacgtaa 721 ttccagcaat taagggtttt tttcattcca tttctttatc agaatcaagc tctcttcaag 781 atgcattaag gttattaact ttatggttta cttttggtgg tattccagaa gcaacccaag 841 ctatgcacga gggtttcaac ctaatccaaa taggcacatg gttagaagtg ttgccacagt 901 taatttctag aattcatcaa cccaatcaaa ttgttagtag gtcattactc tccctattat 961 ctgatctagg taaggctcat ccgcaggcat tagtgtaccc cttaatggtt gcgattaaat 1021 ccgaatctct ctcacgacag aaagcagctt tgtccatcat agaaaagatg agaatacata 1081 gtccagtttt ggtcgaccag gctgaacttg tcagccacga attgatacgt atggcggtgc 1141 tttggcatga gcaatggtat gagggtctgg atgacgccag taggcagttt tttggagaac 1201 ataataccga aaaaatgttt gctgctttag agcctctgta cgaaatgctg aagagaggac 1261 cggaaacttt gagggaaata tcgttccaaa attcttttgg tagggacttg aatgacgctt 1321 acgaatggct gatgaattac aaaaaatcta aagatgttag taatttaaac caagcgtggg 1381 acatttacta taatgttttc aggaaaattg gtaaacagtt gccacaatta caaactcttg 1441 aactacaaca tgtgtcgcca aaactactat ctgcgcatga tttggaattg gctgtccccg 1501 ggacccgtgc aagtggtgga aaaccaattg ttaaaatatc taaattcgag ccagtatttt 1561 cagtaatctc atccaaacaa agaccgagaa agttttgtat caagggtagt gatggtaaag 1621 attataagta tgtgttgaaa ggacatgaag acattagaca ggatagcttg gtcatgcaat 1681 tattcggact agttaacacg cttttgcaaa atgacgctga gtgctttaga aggcatctag 1741 atatccagca atatccagca atcccattat ctccgaagtc tgggttactg ggttgggtac 1801 cgaatagtga cacgttccat gtattaatta gggagcatag agaagccaaa aaaattcctt 1861 taaacattga gcattgggtc atgttacaaa tggcacctga ttatgacaat ttaacgttgt 1921 tgcagaaagt agaagtcttc acttacgccc taaataatac ggagggacaa gatctttata 1981 aggtgttatg gctgaagagt aggtcatcgg aaacgtggtt ggagcgtaga actacttaca 2041 ctcgatcgct agccgtgatg tccatgaccg gttatatatt ggggttaggt gaccgccacc 2101 ctagtaattt gatgttggat agaatcactg ggaaagtcat tcatattgat tttggtgatt 2161 gtttcgaggc tgctatatta agagaaaaat tccccgaaaa agtacctttt agattaacta 2221 gaatgttaac atatgcaatg gaagtgagtg gaattgaagg tagcttccgt attacttgtg 2281 agaatgttat gaaggtactt agagataaca agggttcatt aatggcaatc cttaaagctt 2341 ttgctttcga tcctttgatc aattggggtt ttgacttacc aacaaagaaa attgaggaag 2401 aaacgggcat tcaacttccc gtgatgaatg ccaatgagct attgagtaat ggggctatta 2461 ccgaagaaga agttcaaagg gtggaaaacg agcacaagaa tgccattcga aatgcaaggg 2521 ccatgttggt attgaagcgc attactgaca aattaacggg gaacgatata agaaggttta 2581 atgacttgga cgttccagaa caagtggata aactaatcca acaagccaca tcagtggaaa 2641 acctatgcca acattatatc ggttggtgtc cattctggta gttttaatgt attgaaaatc 2701 acttagttgt atatagcctg taaaagtgtt gtataaaaat gcatctcatt cattaatgtt 2761 tagttccgag cgtgacgtta ctaactgcga aaaagggcaa ttctgcagat atccatcaca 2821 ctggcggccg ctcgagcatg catctagagg gcccaattcg ccctatagtg agtcgtatta 2881 caattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact 2941 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac 3001 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc 3061 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc 3121 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc 3181 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc 3241 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg 3301 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact 3361 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt 3421 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa 3481 attcagggcg caagggctgc taaaggaagc ggaacacgta gaaagccagt ccgcagaaac 3541 ggtgctgacc ccggatgaat gtcagctact gggctatctg gacaagggaa aacgcaagcg 3601 caaagagaaa gcaggtagct tgcagtgggc ttacatggcg atagctagac tgggcggttt 3661 tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc 3721 cctgcaaagt aaactggatg gctttcttgc cgccaaggat ctgatggcgc aggggatcaa 3781 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg 3841 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa 3901 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg 3961 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt 4021 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa 4081 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatcc caccttgctc 4141 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg 4201 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg 4261 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg 4321 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg 4381 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact 4441 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg 4501 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc 4561 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga attgaaaaag 4621 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg 4681 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt 4741 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt 4801 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt 4861 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa 4921 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag 4981 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac 5041 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac 5101 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac 5161 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac 5221 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact 5281 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg 5341 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt 5401 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat 5461 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta 5521 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa 5581 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 5641 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 5701 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 5761 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc 5821 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 5881 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 5941 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 6001 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 6061 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 6121 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 6181 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 6241 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc 6301 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga 6361 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga 6421 agcggaag //