BBa_K563037 1 BBa_K563037 pEASY+33Tor2(with E->K mutation) 2011-10-04T11:00:00Z 2015-05-08T01:12:42Z pEASY vector is bought from Transgene Company, and we get mutant-33Tor2 by mutating the 33Tor2 from genomic sequence. There are totally four mutation sites as we designed in the complete Tor2 sequence. This BioBrick contains the third part of Tor2 with mutation that from amino acid E to amino acid K. false false _731_ 0 10236 9 It's complicated false We will use overlap PCR to conduct this mutation. After we get this mutant part of Tor2, we can keep it for further connection. false Guo Jing annotation2154711 1 EK mutation site range2154711 1 2333 2335 annotation2154709 1 Ampicillin Resistance ORF range2154709 1 4628 5488 annotation2154707 1 33Tor2 (EK mutation) range2154707 1 294 2792 annotation2154708 1 Kanamycin resistance ORF range2154708 1 3816 4610 BBa_K563037_sequence 1 agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgccaagcttggtaccgagctcggatccactagtaacggccgccagtgtgctggaattgcccttagaatggctcatgatttaggtttggatccaaataatatgatagctcaaagcgttcctcaacaaagcaaaagagtccctcgtcacgttgaagattatactaagcttttagctcgttgtttcttgaagcaaggagaatggagagtttgcttacagcctaaatggagattgagcaatccagattcgatcctaggctcctatttgctcgctacacattttgacaacacatggtacaaagcgtggcataactgggcactggccaattttgaagtcatttctatgctaacatctgtctctaaaaagaaacaggaaggaagtgatgcttcctcggtaactgatattaatgagtttgataatggcatgatcggcgtcaatacatttgatgctaaggaagttcattactcttctaatttaatacacaggcacgtaattccagcaattaagggtttttttcattccatttctttatcagaatcaagctctcttcaagatgcattaaggttattaactttatggtttacttttggtggtattccagaagcaacccaagctatgcacgagggtttcaacctaatccaaataggcacatggttagaagtgttgccacagttaatttctagaattcatcaacccaatcaaattgttagtaggtcattactctccctattatctgatctaggtaaggctcatccgcaggcattagtgtaccccttaatggttgcgattaaatccgaatctctctcacgacagaaagcagctttgtccatcatagaaaagatgagaatacatagtccagttttggtcgaccaggctgaacttgtcagccacgaattgatacgtatggcggtgctttggcatgagcaatggtatgagggtctggatgacgccagtaggcagttttttggagaacataataccgaaaaaatgtttgctgctttagagcctctgtacgaaatgctgaagagaggaccggaaactttgagggaaatatcgttccaaaattcttttggtagggacttgaatgacgcttacgaatggctgatgaattacaaaaaatctaaagatgttagtaatttaaaccaagcgtgggacatttactataatgttttcaggaaaattggtaaacagttgccacaattacaaactcttgaactacaacatgtgtcgccaaaactactatctgcgcatgatttggaattggctgtccccgggacccgtgcaagtggtggaaaaccaattgttaaaatatctaaattcgagccagtattttcagtaatctcatccaaacaaagaccgagaaagttttgtatcaagggtagtgatggtaaagattataagtatgtgttgaaaggacatgaagacattagacaggatagcttggtcatgcaattattcggactagttaacacgcttttgcaaaatgacgctgagtgctttagaaggcatctagatatccagcaatatccagcaatcccattatctccgaagtctgggttactgggttgggtaccgaatagtgacacgttccatgtattaattagggagcatagagaagccaaaaaaattcctttaaacattgagcattgggtcatgttacaaatggcacctgattatgacaatttaacgttgttgcagaaagtagaagtcttcacttacgccctaaataatacggagggacaagatctttataaggtgttatggctgaagagtaggtcatcggaaacgtggttggagcgtagaactacttacactcgatcgctagccgtgatgtccatgaccggttatatattggggttaggtgaccgccaccctagtaatttgatgttggatagaatcactgggaaagtcattcatattgattttggtgattgtttcgaggctgctatattaagagaaaaattccccgaaaaagtaccttttagattaactagaatgttaacatatgcaatggaagtgagtggaattgaaggtagcttccgtattacttgtgagaatgttatgaaggtacttagagataacaagggttcattaatggcaatccttaaagcttttgctttcgatcctttgatcaattggggttttgacttaccaacaaagaaaattgaggaagaaacgggcattcaacttcccgtgatgaatgccaatgagctattgagtaatggggctattaccgaagaagaagttcaaagggtggaaaacgagcacaagaatgccattcgaaatgcaagggccatgttggtattgaagcgcattactgacaaattaacggggaacgatataagaaggtttaatgacttggacgttccagaacaagtggataaactaatccaacaagccacatcagtggaaaacctatgccaacattatatcggttggtgtccattctggtagttttaatgtattgaaaatcacttagttgtatatagcctgtaaaagtgttgtataaaaatgcatctcattcattaatgtttagttccgagcgtgacgttactaactgcgaaaaagggcaattctgcagatatccatcacactggcggccgctcgagcatgcatctagagggcccaattcgccctatagtgagtcgtattacaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaattcagggcgcaagggctgctaaaggaagcggaacacgtagaaagccagtccgcagaaacggtgctgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcggttttatggacagcaagcgaaccggaattgccagctggggcgccctctggtaaggttgggaagccctgcaaagtaaactggatggctttcttgccgccaaggatctgatggcgcaggggatcaagatctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatcccaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgcgcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgcca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igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z