LOCUS BBa_K563053 5856 bp DNA linear 22-Nov-2024 DEFINITION vector pYE, designed for inducible expression of recombinant proteins in S.cerevisivae. ACCESSION BBa_K563053 VERSION BBa_K563053.1 FEATURES Location/Qualifiers promoter 1..451 /label=GAL1 misc_feature 608..856 /label=CYC1 transcription terminator CDS 1856..2716 /label=Amplicillin CDS 2734..3841 /label=URA3 gene misc_feature 1038..1711 /label=pUC origin misc_feature 5384..5839 /label=f1 origin ORIGIN 1 acggattaga agccgccgag cgggtgacag ccctccgaag gaagactctc ctccgtgcgt 61 cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga 121 acaataaaga ttctacaata ctagctttta tggttatgaa gaggaaaaat tggcagtaac 181 ctggccccac aaaccttcaa atgaacgaat caaattaaca accataggat gataatgcga 241 ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt tttgatctat 301 taacagatat ataaatgcaa aaactgcata accactttaa ctaatacttt caacattttc 361 ggtttgtatt acttcttatt caaatgtaat aaaagtatca acaaaaaatt gttaatatac 421 ctctatactt taacgtcaag gagaaaaaac cccggatcgg actactagca gctgtaatac 481 gactcactat agggaatatt aagcttggta ccgagctcgg atccactagt aacggccgcc 541 agtgtgctgg aattctgcag atatccatca cactggcggc cgctcgagca tgcatctaga 601 gggccgcatc atgtaattag ttatgtcacg cttacattca cgccctcccc ccacatccgc 661 tctaaccgaa aaggaaggag ttagacaacc tgaagtctag gtccctattt atttttttat 721 agttatgtta gtattaagaa cgttatttat atttcaaatt tttctttttt ttctgtacag 781 acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt gagaaggttt tgggacgctc 841 gaaggcttta atttgcggcc ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 901 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 961 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 1021 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa gcccaggaac cgtaaaaagg 1081 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 1141 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 1201 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 1261 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 1321 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 1381 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 1441 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 1501 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 1561 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 1621 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 1681 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 1741 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 1801 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 1861 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 1921 cctgactccc cgtcgtgtag ataactacga tacgggagcg cttaccatct ggccccagtg 1981 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 2041 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc attcagtcta 2101 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 2161 ttggcattgc tacaggcatc gtggtgtcac tctcgtcgtt tggtatggct tcattcagct 2221 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 2281 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 2341 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 2401 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 2461 gcccggcgtc aatacgggat aatagtgtat cacatagcag aactttaaaa gtgctcatca 2521 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 2581 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 2641 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 2701 aatgttgaat actcatactc ttcctttttc aatgggtaat aactgatata attaaattga 2761 agctctaatt tgtgagttta gtatacatgc atttacttat aatacagttt tttagttttg 2821 ctggccgcat cttctcaaat atgcttccca gcctgctttt ctgtaacgtt caccctctac 2881 cttagcatcc cttccctttg caaatagtcc tcttccaaca ataataatgt cagatcctgt 2941 agagaccaca tcatccacgg ttctatactg ttgacccaat gcgtctccct tgtcatctaa 3001 acccacaccg ggtgtcataa tcaaccaatc gtaaccttca tctcttccac ccatgtctct 3061 ttgagcaata aagccgataa caaaatcttt gtcgctcttc gcaatgtcaa cagtaccctt 3121 agtatattct ccagtagata gggagccctt gcatgacaat tctgctaaca tcaaaaggcc 3181 tctaggttcc tttgttactt cttctgccgc ctgcttcaaa ccgctaacaa tacctgggcc 3241 caccacaccg tgtgcattcg taatgtctgc ccattctgct attctgtata cacccgcaga 3301 gtactgcaat ttgactgtat taccaatgtc agcaaatttt ctgtcttcga agagtaaaaa 3361 attgtacttg gcggataatg cctttagcgg cttaactgtg ccctccatgg aaaaatcagt 3421 caagatatcc acatgtgttt ttagtaaaca aattttggga cctaatgctt caactaactc 3481 cagtaattcc ttggtggtac gaacatccaa tgaagcacac aagtttgttt gcttttcgtg 3541 catgatatta aatagcttgg cagcaacagg actaggatga gtagcagcac gttccttata 3601 tgtagctttc gacatgattt atcttcgttt cctgcaggtt tttgttctgt gcagttgggt 3661 taagaatact gggcaatttc atgtttcttc aacactacat atgcgtatat ataccaatct 3721 aagtctgtgc tccttccttc gttcttcctt ctgttcggag attaccgaat caaaaaaatt 3781 tcaaagaaac cgaaatcaaa aaaaagaata aaaaaaaaat gatgaattga attgaaaagc 3841 tagcttatcg atgataagct gtcaaagatg agaattaatt ccacggacta tagactatac 3901 tagatactcc gtctactgta cgatacactt ccgctcaggt ccttgtcctt taacgaggcc 3961 ttaccactct tttgttactc tattgatcca gctcagcaaa ggcagtgtga tctaagattc 4021 tatcttcgcg atgtagtaaa actagctaga ccgagaaaga gactagaaat gcaaaaggca 4081 cttctacaat ggctgccatc attattatcc gatgtgacgc tgcagcttct caatgatatt 4141 cgaatacgct ttgaggagat acagcctaat atccgacaaa ctgttttaca gatttacgat 4201 cgtacttgtt acccatcatt gaattttgaa catccgaacc tgggagtttt ccctgaaaca 4261 gatagtatat ttgaacctgt ataataatat atagtctagc gctttacgga agacaatgta 4321 tgtatttcgg ttcctggaga aactattgca tctattgcat aggtaatctt gcacgtcgca 4381 tccccggttc attttctgcg tttccatctt gcacttcaat agcatatctt tgttaacgaa 4441 gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca 4501 aagaatctga gctgcatttt tacagaacag aaatgcaacg cgaaagcgct attttaccaa 4561 cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa cgcgacgaga gcgctaattt 4621 ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg agagcgctat 4681 tttaccaaca aagaatctat acttcttttt tgttctacaa aaatgcatcc cgagagcgct 4741 atttttctaa caaagcatct tagattactt tttttctcct ttgtgcgctc tataatgcag 4801 tctcttgata actttttgca ctgtaggtcc gttaaggtta gaagaaggct actttggtgt 4861 ctattttctc ttccataaaa aaagcctgac tccacttccc gcgtttactg attactagcg 4921 aagctgcggg tgcatttttt caagataaag gcatccccga ttatattcta taccgatgtg 4981 gattgcgcat actttgtgaa cagaaagtga tagcgttgat gattcttcat tggtcagaaa 5041 attatgaacg gtttcttcta ttttgtctct atatactacg tataggaaat gtttacattt 5101 tcgtattgtt ttcgattcac tctatgaata gttcttacta caattttttt gtctaaagag 5161 taatactaga gataaacata aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc 5221 gaaaggtgga tgggtaggtt atatagggat atagcacaga gatatatagc aaagagatac 5281 ttttgagcaa tgtttgtgga agcggtattc gcaatgggaa gctccacccc ggttgataat 5341 cagaaaagcc ccaaaaacag gaagattgta taagcaaata tttaaattgt aaacgttaat 5401 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa cgaatagccc 5461 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt 5521 ccagtttcca acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa 5581 agggtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg 5641 tcgaggtgcc gtaaagcagt aaatcggaag ggtaaacgga tgcccccatt tagagcttga 5701 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggggct 5761 agggcggtgg gaagtgtagg ggtcacgctg ggcgtaacca ccacacccgc cgcgcttaat 5821 ggggcgctac agggcgcgtg gggatgatcc actagt //