LOCUS BBa_K563054 5009 bp DNA linear 23-Dec-2024 DEFINITION Vector pYD, and can transfer into nucles of S.cerevisivae ACCESSION BBa_K563054 VERSION BBa_K563054.1 FEATURES Location/Qualifiers promoter 1..451 /label=GAL1 promoter 475..494 /label=T7 promoter CDS 2931..3791 /label=Amppicillin ORIGIN 1 acggattaga agccgccgag cgggtgacag ccctccgaag gaagactctc ctccgtgcgt 61 cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga 121 acaataaaga ttctacaata ctagctttta tggttatgaa gaggaaaaat tggcagtaac 181 ctggccccac aaaccttcaa atgaacgaat caaattaaca accataggat gataatgcga 241 ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt tttgatctat 301 taacagatat ataaatgcaa aaactgcata accactttaa ctaatacttt caacattttc 361 ggtttgtatt acttcttatt caaatgtaat aaaagtatca acaaaaaatt gttaatatac 421 ctctatactt taacgtcaag gagaaaaaac cccggatcgg actactagca gctgtaatac 481 gactcactat agggaatatt aagctaattc tacttcatac attttcaatt aagatgcagt 541 tacttcgctg tttttcaata ttttctgtta ttgcttcagt tttagcacag gaactgacaa 601 ctatatgcga gcaaatcccc tcaccaactt tagaatcgac gccgtactct ttgtcaacga 661 ctactatttt ggccaacggg aaggcaatgc aaggagtttt tgaatattac aaatcagtaa 721 cgtttgtcag taattgcggt tctcacccct caacaactag caaaggcagc cccataaaca 781 cacagtatgt ttttaagctt ctgcaggcta gtggtggtgg tggttctggt ggtggtggtt 841 ctggtggtgg tggttctgct agcatgactg gtggacagca aatgggtcgg gatctgtacg 901 acgatgacga taaggtacca ggatccagtg tggtggaatt ctgcagatat ccagcacagt 961 ggcggccgct cgagtctaga gggcccttcg aaggtaagcc tatccctaac cctctcctcg 1021 gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgagtttaa acccgctgat 1081 ctgataacaa cagtgtagat gtaacaaaat cgactttgtt cccactgtac ttttagctcg 1141 tacaaaatac aatatacttt tcatttctcc gtaaacaaca tgttttccca tgtaatatcc 1201 ttttctattt ttcgttccgt taccaacttt acacatactt tatatagcta ttcacttcta 1261 tacactaaaa aactaagaca attttaattt tgctgcctgc catatttcaa tttgttataa 1321 attcctataa tttatcctat tagtagctaa aaaaagatga atgtgaatcg aatcctaaga 1381 gaattgggca agtgcacaaa caatacttaa ataaatacta ctcagtaata acctatttct 1441 tagcattttt gacgaaattt gctattttgt tagagtcttt tacaccattt gtctccacac 1501 ctccgcttac atcaacacca ataacgccat ttaatctaag cgcatcacca acattttctg 1561 gcgtcagtcc accagctaac ataaaatgta agctctcggg gctctcttgc cttccaaccc 1621 agtcagaaat cgagttccaa tccaaaagtt cacctgtccc acctgcttct gaatcaaaca 1681 agggaataaa cgaatgaggt ttctgtgaag ctgcactgag tagtatgttg cagtcttttg 1741 gaaatacgag tcttttaata actggcaaac cgaggaactc ttggtattct tgccacgact 1801 catctccgtg cagttggacg atatcaatgc cgtaatcatt gaccagagcc aaaacatcct 1861 ccttaggttg attacgaaac acgccaacca agtatttcgg agtgcctgaa ctatttttat 1921 atgcttttac aagacttgaa attttccttg caataaccgg gtcaattgtt ctctttctat 1981 tgggcacaca tataataccc agcaagtcag catcggaatc tagagcacat tctgcggcct 2041 ctgtgctctg caagccgcaa actttcacca atggaccaga actacctgtg aaattaataa 2101 cagacatact ccaagctgcc tttgtgtgct taatcacgta tactcacgtg ctcaatagtc 2161 accaatgccc tccctcttgg ccctctcctt ttcttttttc gaccgaattt cttgaagacg 2221 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta 2281 ggacggatcg cttgcctgta acttacacgc gcctcgtatc ttttaatgat ggaataattt 2341 gggaatttac tctgtgttta tttattttta tgttttgtat ttggatttta gaaagtaaat 2401 aaagaaggta gaagagttac ggaatgaaga aaaaaaaata aacaaaggtt taaaaaattt 2461 caacaaaaag cgtactttac atatatattt attagacaag aaaagcagat taaatagata 2521 tacattcgat taacgataag taaaatgtaa aatcacagga ttttcgtgtg tggtcttcta 2581 cacagacaag atgaaacaat tcggcattaa tacctgagag caggaagagc aagataaaag 2641 gtagtatttg ttggcgatcc ccctagagtc ttttacatct tcggaaaaca aaaactattt 2701 tttctttaat ttcttttttt actttctatt tttaatttat atatttatat taaaaaattt 2761 aaattataat tatttttata gcacgtgatg aaaaggaccc aggtggcact tttcggggaa 2821 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 2881 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc 2941 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc 3001 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt 3061 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt 3121 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg 3181 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact 3241 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg 3301 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga 3361 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg 3421 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa 3481 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac 3541 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc 3601 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca 3661 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggca 3721 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta 3781 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc 3841 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 3901 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 3961 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 4021 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 4081 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact 4141 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 4201 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 4261 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 4321 cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag 4381 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 4441 agcttccagg ggggaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 4501 ttgagcgtcg atttttgtga tgctcgtcag gggggccgag cctatggaaa aacgccagca 4561 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg 4621 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc 4681 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa 4741 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt 4801 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttac ctcactcatt 4861 aggcacccca ggctttacac tttatgcttc cggctcctat gttgtgtgga attgtgagcg 4921 gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc ggaattaacc 4981 ctcactaaag ggaacaaaag ctggctagt //