LOCUS BBa_K563057 8616 bp DNA linear 22-Nov-2024 DEFINITION yeast vector with CIT2-Tor ACCESSION BBa_K563057 VERSION BBa_K563057.1 FEATURES Location/Qualifiers promoter 1..489 /label=promoter of CIT2 promoter 1..490 /label=BBa_K563003 misc_feature 5323..5325 /label=L1415P mutation CDS 499..8616 /label=BBa_K563011 ORIGIN 1 ccacaacata agccgctttg gagtgttgaa caaatccgtc cttgggtcat tcaatcaatg 61 gcttggcggt atctcaaaag agcgcaaact aatagcgcgc acattcgacg catttatccg 121 gtggtcatcg actaggggcg aagaggtcac gacctatttt ttcttgcaga aaaaaagtgt 181 gaccttttcc gtagctagac gtctatcagg gcgtcagcaa tgggaggcac agcggaaaaa 241 caataacaat ggtaagcgca attacctttt gagcgttaca ttcgtatgaa attggtgacg 301 ttaatctaaa gatagtcatg ctctcaaaag ggcccattat tctcgacgtt gagcgtatat 361 aagactatta aaacttggtt ctttagatat ggtgttcgtt cctcattatt aagtttcagg 421 gaacaatatc aacacatatc ataacaggtt ctcaaaactt tttgttttaa taatactagt 481 aacaagaaaa tactagagca cagcaattaa aagtcggaga acctgactga aaattcatga 541 atctcttcat ttctatagcc tttcctctat gcatttgtat tatatattta ttaccgtcat 601 tttttacata ctgctgcatt ttggcgccag tgataagtgg caaacaattc gacggaatcg 661 tggtaattat accacgttac tctataacat catgatattg caattaatca aacatacatt 721 taatcttaat gctattagct tactacaact cttttcttta agttatatcg tatatttctt 781 gggcgatgtc agaatattta cccggatatt cctttttaag cactgaatat gtttgaatag 841 agactgacat atatggcagc aattaaaatt ggaagaaatg taatgacagt aggaaagacc 901 aatttttatc atcgtgacac caatcacttc cttaactgag ctttacttgt atttatttac 961 aggtagatta ggagcagtag aaagggaaaa tataccgggt gcataaagag catagtcatt 1021 aagatcaaat agttatcttt ctcaaagaga tttctgatct ttactttccc catatgaaaa 1081 atgaataaat acattaacaa atacaccacg ccacctaact tattgtcttt acgacaaagg 1141 gccgaaggca aacacagaac aagaaagaaa cttacacaca aatcgcactc ccacgatgat 1201 gagatgtcaa ctacttcaaa cacagattcc aatcacaatg ggcccaatga ctctggtaga 1261 gtgatcactg gttctgctgg tcatattggt aaaatatcct ttgtagattc agaactagat 1321 acaacatttt ctactttaaa tttgattttt gataaactta aaagcgatgt gccacaagaa 1381 cgagcctctg gcgctaatga attaagcact actttgacct cattagcaag ggaagtatct 1441 gctgagcaat ttcaaaggtt tagcaacagt ttaaacaata agatatttga acttattcac 1501 gggtttactt caagtgagaa gataggtggt attcttgctg ttgatactct gatctcattc 1561 tacctgagta cagaggagct gccaaaccaa acttcaagac tggcgaacta tttacgtgtt 1621 ttaattccat ccagtgacat tgaagttatg agattagcgg ctaacacctt aggtagattg 1681 accgtgccag gtggtacatt aacatcagat ttcgtcgaat ttgaggtcag aacttgcatt 1741 gattggctta ctctgacagc agataataac tcatcgagct ctaagttgga atacaggaga 1801 catgctgcgc tattaatcat aaaggcatta gcagacaatt caccctatct tttataccct 1861 tacgttaact ctatcttaga caatatttgg gtgccattaa gggatgcaaa gttaattata 1921 cgattagatg ccgcagtggc attgggtaaa tgtcttacta ttattcagga tagagaccct 1981 gctttgggaa aacagtggtt tcaaagatta tttcaaggtt gtacacatgg cttaagtctc 2041 aatacgaatg attcagtgca tgctactctg ttggtatttc gagaattact cagcttgaaa 2101 gcaccttatc tcagggataa atatgatgat atttacaaat ctactatgaa gtacaaggaa 2161 tataaatttg atgttataag gagagaagtt tatgctattt tacctctttt agctgctttt 2221 gaccctgcca ttttcacaaa gaaatatctc gataggataa tggttcatta tttaagatat 2281 ttgaagaaca tcgatatgaa tgctgcaaat aattcggata aaccttttat attagtttct 2341 ataggtgata ttgcatttga agttggttcg agcatttcac cctatatgac acttattctg 2401 gataatatta gggaaggctt aagaacgaaa ttcaaagtta gaaaacaatt cgagaaggat 2461 ttattttatt gcattggtaa attagcttgt gctttgggcc cagcttttgc taagcacttg 2521 aacaaagatc ttcttaattt gatgttaaac tgtccaatgt ccgaccatat gcaggagact 2581 ttaatgatcc ttaacgagaa aataccctct ttggaatcta ccgttaattc gaggatacta 2641 aatttactgt cgatatcctt atctggtgaa aaatttattc aatcaaacca atacgatttt 2701 aataatcaat tttccattga aaaggctcgt aaatcaagaa accaaagttt catgaaaaaa 2761 actggtgaat ctaatgacga tattacagat gcccaaattt tgattcagtg ttttaaaatg 2821 ctgcaactaa ttcatcatca atattccttg acggagtttg ttaggcttat aaccatttct 2881 tacattgagc atgaggattc gtctgtcaga aaattggcag cattaacgtc gtgtgattta 2941 tttatcaaag acgatatatg taaacaaaca tcagttcatg ctttacactc ggtttctgaa 3001 gtgctaagta agctattaat gatcgcaata actgatccgg ttgcagaaat tagattggaa 3061 attcttcagc atttggggtc aaattttgat cctcaattgg cccaaccaga caatttacgc 3121 ctacttttca tggcgctgaa cgatgagatt tttggtattc aattggaagc tatcaaaata 3181 ataggcagat tgagttctgt caaccccgct tatgtagttc cttctttgag gaaaacttta 3241 ctggaactat taacgcaatt gaagttctca aatatgccaa aaaaaaagga ggaaagtgca 3301 actctattat gtacgctgat aaattccagc gatgaagtag cgaaacctta tattgatcct 3361 attctagacg tcattcttcc taaatgccag gatgcttcat ctgccgtagc atccaccgct 3421 ttaaaggttt tgggtgaact atctgttgtt ggaggaaaag aaatgacgcg ttacttaaag 3481 gaattgatgc cattgatcat taacacattt caggaccaat caaactcttt taaaagagat 3541 gccgccttaa caacattagg acagctggct gcttcctctg gttatgttgt tggcccttta 3601 ctagactacc cagagttact tggcattttg ataaatattc ttaagactga aaacaaccct 3661 catatcaggc gtggaactgt tcgtttgatt ggtatattag gcgctcttga tccatataag 3721 cacagagaaa tagaagtcac atcaaactca aagagttcag tagagcaaaa tgctccttca 3781 atcgacatcg cattgctaat gcaaggggta tctccatcca acgatgaata ttaccccact 3841 gtagttatcc acaatctgat gaagatattg aatgatccat cgttgtcaat ccatcacacg 3901 gctgctattc aagctattat gcatattttt caaaaccttg gtttacgatg tgtctccttt 3961 ttggatcaaa ttattccagg tatcatttta gtcatgcgtt catgcccgcc gtcccaactt 4021 gacttttatt ttcagcaact gggatctctc atctcaattg tcaagcaaca tattaggccc 4081 catgtcgaga aaatttatgg tgtgatcagg gagtttttcc cgatcattaa actacaaatc 4141 acaattattt ctgtcataga atcgatatct aaggctctgg aaggtgagtt taaaagattt 4201 gttcccgaga ctctaacctt tttccttgat attcttgaga acgaccagtc taataaaagg 4261 atcgttccga ttcgtatatt aaaatctttg gttacttttg ggccgaatct agaagactat 4321 tcccatttga ttatgcctat cgttgttaga atgactgagt attctgctgg aagtctaaag 4381 aaaatctcca ttataacttt gggtagatta gcaaagaata tcaacctctc tgaaatgtca 4441 tcaagaattg ttcaggcgtt ggtaagaatt ttgaataatg gggatagaga actaacaaaa 4501 gcaaccatga atacgctaag tttgctcctt ttacaactag gtaccgactt tgtggtcttt 4561 gtgccagtga ttaacaaggc gttattgagg aataggattc agcattcagt gtacgatcaa 4621 ctggttaata aattactgaa caatgaatgc ttgccaacaa atatcatatt tgacaaggag 4681 aacgaagtac ctgaaaggaa aaattatgaa gacgaaatgc aagtaacgaa attaccggta 4741 aaccaaaata tcctaaagaa tgcatggtat tgttctcaac agaagaccaa agaagattgg 4801 caagaatgga taagaaggct atctattcag cttctaaagg aatcaccttc agcttgtcta 4861 cgatcctgtt cgagtttagt cagcgtttat tatccgttgg cgagagaatt gtttaatgct 4921 tcattctcaa gttgctgggt tgagcttcaa acgtcatacc aagaggattt gattcaagca 4981 ttatgcaagg ctttatcatc ctctgaaaac ccacccgaga tttatcaaat gttgttaaat 5041 ttagtggaat ttatggagca cgatgacaaa ccattgccta tcccaatcca tacattaggt 5101 aagtatgccc aaaaatgtca tgcttttgcg aaggcactac attacaaaga ggtagaattc 5161 ttagaagagc cgaaaaattc aacaatcgag gcattgatta gcattaataa tcaacttcac 5221 caaactgatt ctgctattgg tattttgaag catgcgcaac aacacaatga attgcagctg 5281 aaggaaactt ggtatgaaaa acttcaacgt tgggaggatg ctccagcagc atataatgag 5341 aaggaggcag caggagaaga ttcggttgaa gtgatgatgg gaaaattaag atcgttatat 5401 gcccttggag agtgggaaga gctttctaaa ttggcatctg aaaagtgggg cacggcaaaa 5461 cccgaagtga agaaggcaat ggcgcctttg gctgccggcg ctgcctgggg tttggagcaa 5521 tgggatgaaa tagcccagta tactagcgtc atgaaatcgc agtctccaga taaagaattc 5581 tatgatgcaa ttttatgttt gcataggaat aattttaaga aggcggaagt tcacatcttt 5641 aatgcaaggg atcttctagt tactgaattg tcagctcttg ttaatgaaag ctacaataga 5701 gcatataatg ttgttgttag agcgcagatt atagcagagt tggaggaaat catcaaatat 5761 aagaagttgc cacaaaattc agataaacgt ctaactatga gagaaacttg gaataccaga 5821 ttactgggct gtcaaaaaaa tattgatgtg tggcaaagaa ttctgcgtgt cagatcattg 5881 gtgataaagc caaaggagga tgctcaagtg aggattaagt ttgccaactt atgcagaaaa 5941 tcgggtagga tggcgctagc taaaaaagtc ttaaatacat tgcttgaaga aacagatgac 6001 ccagatcatc ctaatactgc taaggcatcc cctccagttg tttatgcaca actgaagtac 6061 ttgtgggcta cggggttgca agatgaggct ttgaagcaat taattaattt cacatctaga 6121 atggctcatg atttaggttt ggatccaaat aatatgatag ctcaaagcgt tcctcaacaa 6181 agcaaaagag tccctcgtca cgttgaagat tatactaagc ttttagctcg ttgtttcttg 6241 aagcaaggag aatggagagt ttgcttacag cctaaatgga gattgagcaa tccagattcg 6301 atcctaggct cctatttgct cgctacacat tttgacaaca catggtacaa agcgtggcat 6361 aactgggcac tggccaattt tgaagtcatt tctatgctaa catctgtctc taaaaagaaa 6421 caggaaggaa gtgatgcttc ctcggtaact gatattaatg agtttgataa tggcatgatc 6481 ggcgtcaata catttgatgc taaggaagtt cattactctt ctaatttaat acacaggcac 6541 gtaattccag caattaaggg tttttttcat tccatttctt tatcagaatc aagctctctt 6601 caagatgcat taaggttatt aactttatgg tttacttttg gtggtattcc agaagcaacc 6661 caagctatgc acgagggttt caacctaatc caaataggca catggttaga agtgttgcca 6721 cagttaattt ctagaattca tcaacccaat caaattgtta gtaggtcatt actctcccta 6781 ttatctgatc taggtaaggc tcatccgcag gcattagtgt accccttaat ggttgcgatt 6841 aaatccgaat ctctctcacg acagaaagca gctttgtcca tcatagaaaa gatgagaata 6901 catagtccag ttttggtcga ccaggctgaa cttgtcagcc acgaattgat acgtatggcg 6961 gtgctttggc atgagcaatg gtatgagggt ctggatgacg ccagtaggca gttttttgga 7021 gaacataata ccgaaaaaat gtttgctgct ttagagcctc tgtacgaaat gctgaagaga 7081 ggaccggaaa ctttgaggga aatatcgttc caaaattctt ttggtaggga cttgaatgac 7141 gcttacgaat ggctgatgaa ttacaaaaaa tctaaagatg ttagtaattt aaaccaagcg 7201 tgggacattt actataatgt tttcaggaaa attggtaaac agttgccaca attacaaact 7261 cttgaactac aacatgtgtc gccaaaacta ctatctgcgc atgatttgga attggctgtc 7321 cccgggaccc gtgcaagtgg tggaaaacca attgttaaaa tatctaaatt cgagccagta 7381 ttttcagtaa tctcatccaa acaaagaccg agaaagtttt gtatcaaggg tagtgatggt 7441 aaagattata agtatgtgtt gaaaggacat gaagacatta gacaggatag cttggtcatg 7501 caattattcg gactagttaa cacgcttttg caaaatgacg ctgagtgctt tagaaggcat 7561 ctagatatcc agcaatatcc agcaatccca ttatctccga agtctgggtt actgggttgg 7621 gtaccgaata gtgacacgtt ccatgtatta attagggagc atagagaagc caaaaaaatt 7681 cctttaaaca ttgagcattg ggtcatgtta caaatggcac ctgattatga caatttaacg 7741 ttgttgcaga aagtagaagt cttcacttac gccctaaata atacggaggg acaagatctt 7801 tataaggtgt tatggctgaa gagtaggtca tcggaaacgt ggttggagcg tagaactact 7861 tacactcgat cgctagccgt gatgtccatg accggttata tattggggtt aggtgaccgc 7921 caccctagta atttgatgtt ggatagaatc actgggaaag tcattcatat tgattttggt 7981 gattgtttcg aggctgctat attaagagaa aaattccccg aaaaagtacc ttttagatta 8041 actagaatgt taacatatgc aatggaagtg agtggaattg aaggtagctt ccgtattact 8101 tgtgagaatg ttatgaaggt acttagagat aacaagggtt cattaatggc aatccttgaa 8161 gcttttgctt tcgatccttt gatcaattgg ggttttgact taccaacaaa gaaaattgag 8221 gaagaaacgg gcattcaact tcccgtgatg aatgccaatg agctattgag taatggggct 8281 attaccgaag aagaagttca aagggtggaa aacgagcaca agaatgccat tcgaaatgca 8341 agggccatgt tggtattgaa gcgcattact gacaaattaa cggggaacga tataagaagg 8401 tttaatgact tggacgttcc agaacaagtg gataaactaa tccaacaagc cacatcagtg 8461 gaaaacctat gccaacatta tatcggttgg tgtccattct ggtagtttta atgtattgaa 8521 aatcacttag ttgtatatag cctgtaaaag tgttgtataa aaatgcatct cattcattaa 8581 tgtttagttc cgagcgtgac gttactaact gcgaaa //