##gff-version 3
##sequence-region BBa_K564003 1 5363
BBa_K564003	.	promoter	1	2125	.	+	0	ID=BBa_K564001;Name=BBa_K564001
BBa_K564003	.	promoter	2102	2102	.	+	0	ID=annotation2133452;Name=+1;Parent=BBa_K564001
BBa_K564003	.	non_covalent_binding_site	1973	1986	.	+	0	ID=annotation2133842;Name=crp;Parent=BBa_K564001
BBa_K564003	.	promoter	1898	1911	.	+	0	ID=annotation2133841;Name=PrhaS;Parent=BBa_K564001
BBa_K564003	.	promoter	2088	2095	.	+	0	ID=annotation2133453;Name=-10;Parent=BBa_K564001
BBa_K564003	.	non_covalent_binding_site	1898	1911	.	+	0	ID=annotation2133846;Name=RhaR;Parent=BBa_K564001
BBa_K564003	.	CDS	1	849	.	+	0	ID=annotation2133839;Name=rhaR;Parent=BBa_K564001
BBa_K564003	.	CDS	1002	1838	.	+	0	ID=annotation2133840;Name=rhaS;Parent=BBa_K564001
BBa_K564003	.	non_covalent_binding_site	2004	2017	.	+	0	ID=annotation2133843;Name=crp;Parent=BBa_K564001
BBa_K564003	.	non_covalent_binding_site	2055	2068	.	+	0	ID=annotation2133845;Name=RhaS;Parent=BBa_K564001
BBa_K564003	.	non_covalent_binding_site	2023	2036	.	+	0	ID=annotation2133844;Name=RhaS;Parent=BBa_K564001
BBa_K564003	.	non_covalent_binding_site	1931	1944	.	+	0	ID=annotation2133847;Name=RhaR;Parent=BBa_K564001
BBa_K564003	.	non_covalent_binding_site	2055	2068	.	+	0	ID=annotation2133454;Name=RhaS;Parent=BBa_K564001
BBa_K564003	.	engineered_region	2134	5363	.	+	0	ID=BBa_I732019;Name=BBa_I732019
BBa_K564003	.	ribosome_entry_site	2134	2145	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I732019
BBa_K564003	.	conserved_region	2138	2141	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K564003	.	CDS	2152	5226	.	+	0	ID=BBa_I732005;Name=BBa_I732005;Parent=BBa_I732019
BBa_K564003	.	point_mutation	5175	5175	.	+	0	ID=annotation1936869;Name=C->T;Parent=BBa_I732005
BBa_K564003	.	stop_codon	5224	5226	.	+	0	ID=annotation1936872;Name=STOP;Parent=BBa_I732005
BBa_K564003	.	start_codon	2152	2154	.	+	0	ID=annotation1936871;Name=START;Parent=BBa_I732005
BBa_K564003	.	CDS	2152	5226	.	+	0	ID=annotation1936870;Name=lacZ;Parent=BBa_I732005
BBa_K564003	.	terminator	5323	5363	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I732019
BBa_K564003	.	engineered_region	5323	5363	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K564003	.	stop_codon	5356	5356	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K564003	.	stem_loop	5330	5349	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K564003	.	polyA_site	5350	5363	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K564003	.	terminator	5235	5314	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I732019
BBa_K564003	.	stem_loop	5246	5289	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K564003	.	engineered_region	5235	5314	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K564003
ttaatcgctttgattactgagatgacgccattggctcggcgtcatcccggtttcccgcgtgaacaccactgaaaaataa
ttactatcttcaaaaccgcactgcatcgaaatctcgctaatcatcagcgggctatgctgaagcagatactgcgcgtggc
aaatacggacctggcgtaaatattgattaatcgtcatcccggtttgggcgcgaaactgctgccgcagcacccgttcgct
gcattgttcctgctggcaaaacgcatccagcgcaaagggacactcaaggctattagccaaagcggtaattaatttatcc
aacagggtttctcgggaggttgccggaagatcgtcggtcgcgtagcgatgccgttttaacgtcatcaccagttgaccaa
acagcaattccgccatctcattcgccagcggatcgcgtccattactctcatgttcaagctggttaatgacctgccgcgc
ctgattcattcccatactgcccagccgccagtgaggatgccactgcgctccctgaaagccgggaatcatcgcctgccag
tttacattgagtttcaaacgctccgggcagtaaataatattttgcagcaccagatcgttaacggaagtataggagtgtt
tgtcttcagcacgaatgtaaaacagatcgccgcgggtaattctgtatgggcgctcattaagaacgtgtaggccattgcc
ccgccagaccatcaccagttcacaaaactcatgcgtatgttccgcaaaaacatcctgtggatagcgatcggcaaccgta
acggcctgctgctcatcagtaaaaaaatcttttttcaaaaggattaactgatttgccaccgcagcacctcatgttgaat
gcttgccggatggcgctacgcttattctgtctaccgctcgccgccgtaggccaggtaaggcgcagccgccatccggtat
taaagggtataattatcagcatattggcgacgaaaacgttgatagcgttcgcgttactggattatcgcatcgcgtccct
gacggatatcccgcggcgaccagttaaattctcggcgaaacagagtcgaaaagtggttactgtcgccaaaaccgcagcg
gtacgcgatttctgtcacgctatgatcgctatggcgcaataaatgccgggctttgatcaaacgcaggcgattaaggtaa
cgttgcggcgtcagcccggtatgctgtttaagctgacgatgcagcgtgcgtagcgacagcgaaaactgttcagctaccg
cctcccagcagacctcctccgcaaaatggtcttccagccatgccatcagttggttgagtttggcgtcgttattggtcgc
gccttccatcaggctactgcggcgtaacaacaccagtagttgcataaacaaaatttcgcgattcgccacggcaggcgca
tccatgccgtctccggcgcgctccatcagaccgaccaactggcgcacctgctgcaacacactctgattcacccgccagt
gagaaggatagtaaccatcctgctcctgcggcagcaattgatccaaccccgccaggaactggaaggcgtccggtgaacg
ccacagtacattcgtcaggcataggttgtcggtatgttcatataaatgccggtcgtgatcgcgaacaaaacacacggta
ccgccgctgatggtatacggctgcccgttgaatacatgaatgcccgtaccgtgttccacaatcacgatttcatgaaaat
catgatgatgttcagggaaagcggcttgcggcagacgaggttcaatcgccacgggcgctttacctgatggaaaaaaatc
cacgctatgcagtacggtcatggttgcttcctcctcaattgaacatgtccgaatagtaattaagggttatcggcctcac
cttaaatttttgacgctaaagcgcgcaaacatggtctttttttcaagaaaaggcgggaaaaagcgggaaatgcggacga
catcacaccggcctattagtagaaactgtgaacgctatcacgttcatctttgccttgttgccagcggctcattttcctg
tcagtaacgagaaggtaggtctttgagggcttttttagactgtgcgcaatgactctaagaaggacctgatttactagag
aaagaggagaaatactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctg
gcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcg
cccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagc
tggctggagtgcgatcttcctgaggccgatactgtcgtcgtcccctcaaactggcagatgcacggttacgatgcgccca
tctacaccaacgtgacctatcccattacggtcaatccgccgtttgttcccacggagaatccgacgggttgttactcgct
cacatttaatgttgatgaaagctggctacaggaaggccagacgcgaattatttttgatggcgttaactcggcgtttcat
ctgtggtgcaacgggcgctgggtcggttacggccaggacagtcgtttgccgtctgaatttgacctgagcgcatttttac
gcgccggagaaaaccgcctcgcggtgatggtgctgcgctggagtgacggcagttatctggaagatcaggatatgtggcg
gatgagcggcattttccgtgacgtctcgttgctgcataaaccgactacacaaatcagcgatttccatgttgccactcgc
tttaatgatgatttcagccgcgctgtactggaggctgaagttcagatgtgcggcgagttgcgtgactacctacgggtaa
cagtttctttatggcagggtgaaacgcaggtcgccagcggcaccgcgcctttcggcggtgaaattatcgatgagcgtgg
tggttatgccgatcgcgtcacactacgtctgaacgtcgaaaacccgaaactgtggagcgccgaaatcccgaatctctat
cgtgcggtggttgaactgcacaccgccgacggcacgctgattgaagcagaagcctgcgatgtcggtttccgcgaggtgc
ggattgaaaatggtctgctgctgctgaacggcaagccgttgctgattcgaggcgttaaccgtcacgagcatcatcctct
gcatggtcaggtcatggatgagcagacgatggtgcaggatatcctgctgatgaagcagaacaactttaacgccgtgcgc
tgttcgcattatccgaaccatccgctgtggtacacgctgtgcgaccgctacggcctgtatgtggtggatgaagccaata
ttgaaacccacggcatggtgccaatgaatcgtctgaccgatgatccgcgctggctaccggcgatgagcgaacgcgtaac
gcgaatggtgcagcgcgatcgtaatcacccgagtgtgatcatctggtcgctggggaatgaatcaggccacggcgctaat
cacgacgcgctgtatcgctggatcaaatctgtcgatccttcccgcccggtgcagtatgaaggcggcggagccgacacca
cggccaccgatattatttgcccgatgtacgcgcgcgtggatgaagaccagcccttcccggctgtgccgaaatggtccat
caaaaaatggctttcgctacctggagagacgcgcccgctgatcctttgcgaatacgcccacgcgatgggtaacagtctt
ggcggtttcgctaaatactggcaggcgtttcgtcagtatccccgtttacagggcggcttcgtctgggactgggtggatc
agtcgctgattaaatatgatgaaaacggcaacccgtggtcggcttacggcggtgattttggcgatacgccgaacgatcg
ccagttctgtatgaacggtctggtctttgccgaccgcacgccgcatccagcgctgacggaagcaaaacaccagcagcag
tttttccagttccgtttatccgggcaaaccatcgaagtgaccagcgaatacctgttccgtcatagcgataacgagctcc
tgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaaca
gttgattgaactgcctgaactaccgcagccggagagcgccgggcaactctggctcacagtacgcgtagtgcaaccgaac
gcgaccgcatggtcagaagccgggcacatcagcgcctggcagcagtggcgtctggcggaaaacctcagtgtgacgctcc
ccgccgcgtcccacgccatcccgcatctgaccaccagcgaaatggatttttgcatcgagctgggtaataagcgttggca
atttaaccgccagtcaggctttctttcacagatgtggattggcgataaaaaacaactgctgacgccgctgcgcgatcag
ttcacccgtgcaccgctggataacgacattggcgtaagtgaagcgacccgcattgaccctaacgcctgggtcgaacgct
ggaaggcggcgggccattaccaggccgaagcagcgttgttgcagtgcacggcagatacacttgctgatgcggtgctgat
tacgaccgctcacgcgtggcagcatcaggggaaaaccttatttatcagccggaaaacctaccggattgatggtagtggt
caaatggcgattaccgttgatgttgaagtggcgagcgatacaccgcatccggcgcggattggcctgaactgccagctgg
cgcaggtagcagagcgggtaaactggctcggattagggccgcaagaaaactatcccgaccgccttactgccgcctgttt
tgaccgctgggatctgccattgtcagacatgtataccccgtacgtcttcccgagcgaaaacggtctgcgctgcgggacg
cgcgaattgaattatggcccacaccagtggcgcggcgacttccagttcaacatcagccgctacagtcaacagcaactga
tggaaaccagccatcgccatctgctgcacgcggaagaaggcacatggctgaatatcgacggtttccatatggggattgg
tggcgacgactcctggagcccgtcagtatcggcggaatttcagctgagcgccggtcgctaccattaccagttggtctgg
tgtcaaaaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt
tgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata