LOCUS BBa_K564008 3842 bp DNA linear 22-Nov-2024 DEFINITION Ptet fused with LacZ ACCESSION BBa_K564008 VERSION BBa_K564008.1 FEATURES Location/Qualifiers promoter 1..624 /label=tet promoter 1..624 /label=BBa_K564018 misc_feature 3654..3654 /label=C->T CDS 631..3705 /label=lacZ misc_feature 631..633 /label=START misc_feature 3703..3705 /label=STOP CDS 631..3705 /label=BBa_I732005 stem_loop 3725..3768 /label=stem_loop misc_feature 3714..3793 /label=BBa_B0010 terminator 3714..3793 /label=BBa_B0010 stem_loop 3809..3828 /label=T7 TE misc_feature 3835..3835 /label=stop polyA_site 3829..3842 /label=polya misc_feature 3802..3842 /label=BBa_B0012 terminator 3802..3842 /label=BBa_B0012 ORIGIN 1 ttaagaccca ctttcacatt taagttgttt ttctaatccg catatgatca attcaaggcc 61 gaataagaag gctggctctg caccttggtg atcaaataat tcgatagctt gtcgtaataa 121 tggcggcata ctatcagtag taggtgtttc cctttcttct ttagcgactt gatgctcttg 181 atcttccaat acgcaaccta aagtaaaatg ccccacagcg ctgagtgcat ataatgcatt 241 ctctagtgaa aaaccttgtt ggcataaaaa ggctaattga ttttcgagag tttcatactg 301 tttttctgta ggccgtgtac ctaaatgtac ttttgctcca tcgcgatgac ttagtaaagc 361 acatctaaaa cttttagcgt tattacgtaa aaaatcttgc cagctttccc cttctaaagg 421 gcaaaagtga gtatggtgcc tatctaacat ctcaatggct aaggcgtcga gcaaagcccg 481 cttatttttt acatgccaat acaatgtagg ctgctctaca cctagcttct gggcgagttt 541 acgggttgtt aaaccttcga ttccgacctc attaagcagc tctaatgcgc tgttaatcac 601 tttactttta tctaatctgc tcattactag atgaccatga ttacggattc actggccgtc 661 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca 721 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa 781 cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc agaagcggtg 841 ccggaaagct ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac 901 tggcagatgc acggttacga tgcgcccatc tacaccaacg tgacctatcc cattacggtc 961 aatccgccgt ttgttcccac ggagaatccg acgggttgtt actcgctcac atttaatgtt 1021 gatgaaagct ggctacagga aggccagacg cgaattattt ttgatggcgt taactcggcg 1081 tttcatctgt ggtgcaacgg gcgctgggtc ggttacggcc aggacagtcg tttgccgtct 1141 gaatttgacc tgagcgcatt tttacgcgcc ggagaaaacc gcctcgcggt gatggtgctg 1201 cgctggagtg acggcagtta tctggaagat caggatatgt ggcggatgag cggcattttc 1261 cgtgacgtct cgttgctgca taaaccgact acacaaatca gcgatttcca tgttgccact 1321 cgctttaatg atgatttcag ccgcgctgta ctggaggctg aagttcagat gtgcggcgag 1381 ttgcgtgact acctacgggt aacagtttct ttatggcagg gtgaaacgca ggtcgccagc 1441 ggcaccgcgc ctttcggcgg tgaaattatc gatgagcgtg gtggttatgc cgatcgcgtc 1501 acactacgtc tgaacgtcga aaacccgaaa ctgtggagcg ccgaaatccc gaatctctat 1561 cgtgcggtgg ttgaactgca caccgccgac ggcacgctga ttgaagcaga agcctgcgat 1621 gtcggtttcc gcgaggtgcg gattgaaaat ggtctgctgc tgctgaacgg caagccgttg 1681 ctgattcgag gcgttaaccg tcacgagcat catcctctgc atggtcaggt catggatgag 1741 cagacgatgg tgcaggatat cctgctgatg aagcagaaca actttaacgc cgtgcgctgt 1801 tcgcattatc cgaaccatcc gctgtggtac acgctgtgcg accgctacgg cctgtatgtg 1861 gtggatgaag ccaatattga aacccacggc atggtgccaa tgaatcgtct gaccgatgat 1921 ccgcgctggc taccggcgat gagcgaacgc gtaacgcgaa tggtgcagcg cgatcgtaat 1981 cacccgagtg tgatcatctg gtcgctgggg aatgaatcag gccacggcgc taatcacgac 2041 gcgctgtatc gctggatcaa atctgtcgat ccttcccgcc cggtgcagta tgaaggcggc 2101 ggagccgaca ccacggccac cgatattatt tgcccgatgt acgcgcgcgt ggatgaagac 2161 cagcccttcc cggctgtgcc gaaatggtcc atcaaaaaat ggctttcgct acctggagag 2221 acgcgcccgc tgatcctttg cgaatacgcc cacgcgatgg gtaacagtct tggcggtttc 2281 gctaaatact ggcaggcgtt tcgtcagtat ccccgtttac agggcggctt cgtctgggac 2341 tgggtggatc agtcgctgat taaatatgat gaaaacggca acccgtggtc ggcttacggc 2401 ggtgattttg gcgatacgcc gaacgatcgc cagttctgta tgaacggtct ggtctttgcc 2461 gaccgcacgc cgcatccagc gctgacggaa gcaaaacacc agcagcagtt tttccagttc 2521 cgtttatccg ggcaaaccat cgaagtgacc agcgaatacc tgttccgtca tagcgataac 2581 gagctcctgc actggatggt ggcgctggat ggtaagccgc tggcaagcgg tgaagtgcct 2641 ctggatgtcg ctccacaagg taaacagttg attgaactgc ctgaactacc gcagccggag 2701 agcgccgggc aactctggct cacagtacgc gtagtgcaac cgaacgcgac cgcatggtca 2761 gaagccgggc acatcagcgc ctggcagcag tggcgtctgg cggaaaacct cagtgtgacg 2821 ctccccgccg cgtcccacgc catcccgcat ctgaccacca gcgaaatgga tttttgcatc 2881 gagctgggta ataagcgttg gcaatttaac cgccagtcag gctttctttc acagatgtgg 2941 attggcgata aaaaacaact gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg 3001 gataacgaca ttggcgtaag tgaagcgacc cgcattgacc ctaacgcctg ggtcgaacgc 3061 tggaaggcgg cgggccatta ccaggccgaa gcagcgttgt tgcagtgcac ggcagataca 3121 cttgctgatg cggtgctgat tacgaccgct cacgcgtggc agcatcaggg gaaaacctta 3181 tttatcagcc ggaaaaccta ccggattgat ggtagtggtc aaatggcgat taccgttgat 3241 gttgaagtgg cgagcgatac accgcatccg gcgcggattg gcctgaactg ccagctggcg 3301 caggtagcag agcgggtaaa ctggctcgga ttagggccgc aagaaaacta tcccgaccgc 3361 cttactgccg cctgttttga ccgctgggat ctgccattgt cagacatgta taccccgtac 3421 gtcttcccga gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta tggcccacac 3481 cagtggcgcg gcgacttcca gttcaacatc agccgctaca gtcaacagca actgatggaa 3541 accagccatc gccatctgct gcacgcggaa gaaggcacat ggctgaatat cgacggtttc 3601 catatgggga ttggtggcga cgactcctgg agcccgtcag tatcggcgga atttcagctg 3661 agcgccggtc gctaccatta ccagttggtc tggtgtcaaa aataatacta gagccaggca 3721 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 3781 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 3841 ta //