LOCUS BBa_K564013 3817 bp DNA linear 22-Nov-2024 DEFINITION Upstream mutated chitoporin part fused with lacZ ACCESSION BBa_K564013 VERSION BBa_K564013.1 FEATURES Location/Qualifiers CDS 1..599 /label=ChiP mutated misc_feature 578..589 /label=changed binding site for ChiX misc_feature 1..599 /label=BBa_K564015 misc_feature 3629..3629 /label=C->T CDS 606..3680 /label=lacZ misc_feature 606..608 /label=START misc_feature 3678..3680 /label=STOP CDS 606..3680 /label=BBa_I732005 stem_loop 3700..3743 /label=stem_loop misc_feature 3689..3768 /label=BBa_B0010 terminator 3689..3768 /label=BBa_B0010 stem_loop 3784..3803 /label=T7 TE misc_feature 3810..3810 /label=stop polyA_site 3804..3817 /label=polya misc_feature 3777..3817 /label=BBa_B0012 terminator 3777..3817 /label=BBa_B0012 ORIGIN 1 gggcgaaagt aggcgagtaa ttttaagttt cgctatgccg gatggggcgt ttacgtcgca 61 tccggcaagg aacagacaaa cagtttcaaa cgctaaattg cctgatgcgc tacgcttatc 121 aggcctacat gatctctgca atatattgag tttgcgtgct tttgtaggcc ggataaggcg 181 ttcacgccgc atccggcaag aaacagcaaa caatccaaaa cgccgcgttc agcggcgttt 241 tttctgcttt tcttcgcgaa ttaattccgc ttcgcaattt atccataaaa taaatttaaa 301 ataacaaaac ataattaaat aaaatgtaac cgctttcatc ttgctggaat ttcacgcttt 361 tattcttctg caagcctttc aaccgcaaac ttaagccttg taacaaaaat catcaaaata 421 tgtgcggttg ctcatgttct tacattctgg ttacagaaag agattgataa ttcgcgtcgc 481 gaaaaatagt ctgttcctgt agtcagcgag acttttctca acgctacttt tttaattttt 541 attttttcgc tgttcacctt tggtgcagca atttatacgt caaggagaat taacccatgt 601 actagatgac catgattacg gattcactgg ccgtcgtttt acaacgtcgt gactgggaaa 661 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta 721 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat 781 ggcgctttgc ctggtttccg gcaccagaag cggtgccgga aagctggctg gagtgcgatc 841 ttcctgaggc cgatactgtc gtcgtcccct caaactggca gatgcacggt tacgatgcgc 901 ccatctacac caacgtgacc tatcccatta cggtcaatcc gccgtttgtt cccacggaga 961 atccgacggg ttgttactcg ctcacattta atgttgatga aagctggcta caggaaggcc 1021 agacgcgaat tatttttgat ggcgttaact cggcgtttca tctgtggtgc aacgggcgct 1081 gggtcggtta cggccaggac agtcgtttgc cgtctgaatt tgacctgagc gcatttttac 1141 gcgccggaga aaaccgcctc gcggtgatgg tgctgcgctg gagtgacggc agttatctgg 1201 aagatcagga tatgtggcgg atgagcggca ttttccgtga cgtctcgttg ctgcataaac 1261 cgactacaca aatcagcgat ttccatgttg ccactcgctt taatgatgat ttcagccgcg 1321 ctgtactgga ggctgaagtt cagatgtgcg gcgagttgcg tgactaccta cgggtaacag 1381 tttctttatg gcagggtgaa acgcaggtcg ccagcggcac cgcgcctttc ggcggtgaaa 1441 ttatcgatga gcgtggtggt tatgccgatc gcgtcacact acgtctgaac gtcgaaaacc 1501 cgaaactgtg gagcgccgaa atcccgaatc tctatcgtgc ggtggttgaa ctgcacaccg 1561 ccgacggcac gctgattgaa gcagaagcct gcgatgtcgg tttccgcgag gtgcggattg 1621 aaaatggtct gctgctgctg aacggcaagc cgttgctgat tcgaggcgtt aaccgtcacg 1681 agcatcatcc tctgcatggt caggtcatgg atgagcagac gatggtgcag gatatcctgc 1741 tgatgaagca gaacaacttt aacgccgtgc gctgttcgca ttatccgaac catccgctgt 1801 ggtacacgct gtgcgaccgc tacggcctgt atgtggtgga tgaagccaat attgaaaccc 1861 acggcatggt gccaatgaat cgtctgaccg atgatccgcg ctggctaccg gcgatgagcg 1921 aacgcgtaac gcgaatggtg cagcgcgatc gtaatcaccc gagtgtgatc atctggtcgc 1981 tggggaatga atcaggccac ggcgctaatc acgacgcgct gtatcgctgg atcaaatctg 2041 tcgatccttc ccgcccggtg cagtatgaag gcggcggagc cgacaccacg gccaccgata 2101 ttatttgccc gatgtacgcg cgcgtggatg aagaccagcc cttcccggct gtgccgaaat 2161 ggtccatcaa aaaatggctt tcgctacctg gagagacgcg cccgctgatc ctttgcgaat 2221 acgcccacgc gatgggtaac agtcttggcg gtttcgctaa atactggcag gcgtttcgtc 2281 agtatccccg tttacagggc ggcttcgtct gggactgggt ggatcagtcg ctgattaaat 2341 atgatgaaaa cggcaacccg tggtcggctt acggcggtga ttttggcgat acgccgaacg 2401 atcgccagtt ctgtatgaac ggtctggtct ttgccgaccg cacgccgcat ccagcgctga 2461 cggaagcaaa acaccagcag cagtttttcc agttccgttt atccgggcaa accatcgaag 2521 tgaccagcga atacctgttc cgtcatagcg ataacgagct cctgcactgg atggtggcgc 2581 tggatggtaa gccgctggca agcggtgaag tgcctctgga tgtcgctcca caaggtaaac 2641 agttgattga actgcctgaa ctaccgcagc cggagagcgc cgggcaactc tggctcacag 2701 tacgcgtagt gcaaccgaac gcgaccgcat ggtcagaagc cgggcacatc agcgcctggc 2761 agcagtggcg tctggcggaa aacctcagtg tgacgctccc cgccgcgtcc cacgccatcc 2821 cgcatctgac caccagcgaa atggattttt gcatcgagct gggtaataag cgttggcaat 2881 ttaaccgcca gtcaggcttt ctttcacaga tgtggattgg cgataaaaaa caactgctga 2941 cgccgctgcg cgatcagttc acccgtgcac cgctggataa cgacattggc gtaagtgaag 3001 cgacccgcat tgaccctaac gcctgggtcg aacgctggaa ggcggcgggc cattaccagg 3061 ccgaagcagc gttgttgcag tgcacggcag atacacttgc tgatgcggtg ctgattacga 3121 ccgctcacgc gtggcagcat caggggaaaa ccttatttat cagccggaaa acctaccgga 3181 ttgatggtag tggtcaaatg gcgattaccg ttgatgttga agtggcgagc gatacaccgc 3241 atccggcgcg gattggcctg aactgccagc tggcgcaggt agcagagcgg gtaaactggc 3301 tcggattagg gccgcaagaa aactatcccg accgccttac tgccgcctgt tttgaccgct 3361 gggatctgcc attgtcagac atgtataccc cgtacgtctt cccgagcgaa aacggtctgc 3421 gctgcgggac gcgcgaattg aattatggcc cacaccagtg gcgcggcgac ttccagttca 3481 acatcagccg ctacagtcaa cagcaactga tggaaaccag ccatcgccat ctgctgcacg 3541 cggaagaagg cacatggctg aatatcgacg gtttccatat ggggattggt ggcgacgact 3601 cctggagccc gtcagtatcg gcggaatttc agctgagcgc cggtcgctac cattaccagt 3661 tggtctggtg tcaaaaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 3721 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 3781 actggctcac cttcgggtgg gcctttctgc gtttata //