BBa_K564017
type: ComponentDefinition
persistent identity: BBa K564017
display id: BBa_K564017
version: 1
was generated by: iGEM to SBOL conversion
description: Upstream mutated chitoporin part fused with lacZ
created: 2011-09-19T11:00:00Z
modified: 2015-05-08T01:12:42Z
mutable provenance: The subparts were prepared: ChiP upstream part from Escherichia Coli W3110 genomic DNA by PCR and lacZ from part BBa_I732005 present in part registry.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: ChiP encodes enterobacterial chitoporin required for uptake of chitin-derived oligosaccharides. This outer membrane porin is required for Salmonella and E.coli growth on chitooligosaccharides as a sole source of both carbon and nitrogen. It is only synthesized in the presence of these substrates. In nature it is regulated by sRNAs.In this biobrick we altered the binding site for ChiP regulator ??? ChiX. It was done in order to show that the regulatory system depends on the specific base pairing between ChiP and its regulator. The upstream part of this gene is fused with reporter gene lacZ to allow checking if the regulation works correctly.
discontinued: true
dominant: false
experience: experience/None
group u list: _732_
m user id: 0
owner id: 8333
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: There are no design considerations.
star: false
is replaced by: ChiPm-lacZ
creator: Anna Lewinska
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0010
component: BBa_I732005
component: BBa_K564015
component: BBa_B0012
sequence annotation: BBa_K564015
sequence annotation: BBa_I732005
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K564017_sequence/1

BBa_B0010
type: Component
persistent identity: component2135260
display id: component2135260
version: 1
top level: BBa_K564017
definition: BBa_B0010
access: public

BBa_I732005
type: Component
persistent identity: component2135259
display id: component2135259
version: 1
top level: BBa_K564017
definition: LacZ
access: public

BBa_K564015
type: Component
persistent identity: component2135254
display id: component2135254
version: 1
top level: BBa_K564017
definition: ChiPm
access: public

BBa_B0012
type: Component
persistent identity: component2135262
display id: component2135262
version: 1
top level: BBa_K564017
definition: BBa_B0012
access: public

BBa_K564015
type: SequenceAnnotation
persistent identity: annotation2135254
display id: annotation2135254
version: 1
top level: BBa_K564017
location: https://synbiohub.org/public/igem/BBa_K564017/annotation2135254/range2135254/1
component: BBa_K564015

https://synbiohub.org/public/igem/BBa_K564017/annotation2135254/range2135254/1
type: Range
persistent identity: range2135254
display id: range2135254
version: 1
top level: BBa_K564017
direction: direction/reverse
start: 1
end: 599
orientation: inline

BBa_I732005
type: SequenceAnnotation
persistent identity: annotation2135259
display id: annotation2135259
version: 1
top level: BBa_K564017
location: https://synbiohub.org/public/igem/BBa_K564017/annotation2135259/range2135259/1
component: BBa_I732005

https://synbiohub.org/public/igem/BBa_K564017/annotation2135259/range2135259/1
type: Range
persistent identity: range2135259
display id: range2135259
version: 1
top level: BBa_K564017
direction: direction/reverse
start: 606
end: 3680
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2135260
display id: annotation2135260
version: 1
top level: BBa_K564017
location: https://synbiohub.org/public/igem/BBa_K564017/annotation2135260/range2135260/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K564017/annotation2135260/range2135260/1
type: Range
persistent identity: range2135260
display id: range2135260
version: 1
top level: BBa_K564017
direction: direction/forward
start: 3689
end: 3768
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2135262
display id: annotation2135262
version: 1
top level: BBa_K564017
location: https://synbiohub.org/public/igem/BBa_K564017/annotation2135262/range2135262/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K564017/annotation2135262/range2135262/1
type: Range
persistent identity: range2135262
display id: range2135262
version: 1
top level: BBa_K564017
direction: direction/forward
start: 3777
end: 3817
orientation: inline

ChiPm-lacZ
type: ComponentDefinition
persistent identity: BBa K564013
display id: BBa_K564013
version: 1
was generated by: iGEM to SBOL conversion
description: Upstream mutated chitoporin part fused with lacZ
created: 2011-09-19T11:00:00Z
modified: 2015-05-08T01:12:42Z
mutable provenance: The subparts were prepared: ChiP upstream part from Escherichia Coli W3110 genomic DNA by PCR and lacZ from part BBa_I732005 present in part registry.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: ChiP encodes enterobacterial chitoporin required for uptake of chitin-derived oligosaccharides. This outer membrane porin is required for Salmonella and E.coli growth on chitooligosaccharides as a sole source of both carbon and nitrogen. It is only synthesized in the presence of these substrates. In nature it is regulated by sRNAs.In this biobrick we altered the binding site for ChiP regulator ??? ChiX. It was done in order to show that the regulatory system depends on the specific base pairing between ChiP and its regulator. The upstream part of this gene is fused with reporter gene lacZ to allow checking if the regulation works correctly.
discontinued: false
dominant: true
experience: experience/None
group u list: _732_
m user id: 0
owner id: 8333
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: There are no design considerations.
star: false
creator: Anna Lewinska
type: DnaRegion
role: partType/Measurement
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_I732005
component: BBa_K564015
sequence annotation: BBa_K564015
sequence annotation: BBa_I732005
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K564013_sequence/1

BBa_B0012
type: Component
persistent identity: component2136030
display id: component2136030
version: 1
top level: ChiPm-lacZ
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2136028
display id: component2136028
version: 1
top level: ChiPm-lacZ
definition: BBa_B0010
access: public

BBa_I732005
type: Component
persistent identity: component2136027
display id: component2136027
version: 1
top level: ChiPm-lacZ
definition: LacZ
access: public

BBa_K564015
type: Component
persistent identity: component2136022
display id: component2136022
version: 1
top level: ChiPm-lacZ
definition: ChiPm
access: public

BBa_K564015
type: SequenceAnnotation
persistent identity: annotation2136022
display id: annotation2136022
version: 1
top level: ChiPm-lacZ
location: https://synbiohub.org/public/igem/BBa_K564013/annotation2136022/range2136022/1
component: BBa_K564015

https://synbiohub.org/public/igem/BBa_K564013/annotation2136022/range2136022/1
type: Range
persistent identity: range2136022
display id: range2136022
version: 1
top level: ChiPm-lacZ
direction: direction/reverse
start: 1
end: 599
orientation: inline

BBa_I732005
type: SequenceAnnotation
persistent identity: annotation2136027
display id: annotation2136027
version: 1
top level: ChiPm-lacZ
location: https://synbiohub.org/public/igem/BBa_K564013/annotation2136027/range2136027/1
component: BBa_I732005

https://synbiohub.org/public/igem/BBa_K564013/annotation2136027/range2136027/1
type: Range
persistent identity: range2136027
display id: range2136027
version: 1
top level: ChiPm-lacZ
direction: direction/reverse
start: 606
end: 3680
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2136028
display id: annotation2136028
version: 1
top level: ChiPm-lacZ
location: https://synbiohub.org/public/igem/BBa_K564013/annotation2136028/range2136028/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K564013/annotation2136028/range2136028/1
type: Range
persistent identity: range2136028
display id: range2136028
version: 1
top level: ChiPm-lacZ
direction: direction/forward
start: 3689
end: 3768
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2136030
display id: annotation2136030
version: 1
top level: ChiPm-lacZ
location: https://synbiohub.org/public/igem/BBa_K564013/annotation2136030/range2136030/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K564013/annotation2136030/range2136030/1
type: Range
persistent identity: range2136030
display id: range2136030
version: 1
top level: ChiPm-lacZ
direction: direction/forward
start: 3777
end: 3817
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

LacZ
type: ComponentDefinition
persistent identity: BBa I732005
display id: BBa_I732005
version: 1
was generated by: iGEM to SBOL conversion
description: lacZ (encoding beta-galactosidase, full-length)
created: 2007-07-05T11:00:00Z
modified: 2015-08-31T04:07:56Z
mutable provenance: PCR amplified and standardized from BL21
owned by: james
owned by: myers
mutable description: LacZ encodes beta-galactosidase, an intracellular enzyme that cleaves the disaccharide lactose into glucose and galactose. Beta-galactosidase can also cleaves X-gal and ONPG into colorful products.
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None
star: true
creator: Zhan Jian
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: START
sequence annotation: lacZ
sequence annotation: C->T
sequence annotation: STOP
sequence: https://synbiohub.org/public/igem/BBa_I732005_sequence/1

START
type: SequenceAnnotation
persistent identity: annotation1936871
display id: annotation1936871
version: 1
top level: LacZ
location: https://synbiohub.org/public/igem/BBa_I732005/annotation1936871/range1936871/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_I732005/annotation1936871/range1936871/1
type: Range
persistent identity: range1936871
display id: range1936871
version: 1
top level: LacZ
direction: direction/forward
start: 1
end: 3
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1936870
display id: annotation1936870
version: 1
top level: LacZ
location: https://synbiohub.org/public/igem/BBa_I732005/annotation1936870/range1936870/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I732005/annotation1936870/range1936870/1
type: Range
persistent identity: range1936870
display id: range1936870
version: 1
top level: LacZ
direction: direction/forward
start: 1
end: 3075
orientation: inline

C->T
type: SequenceAnnotation
persistent identity: annotation1936869
display id: annotation1936869
version: 1
top level: LacZ
location: https://synbiohub.org/public/igem/BBa_I732005/annotation1936869/range1936869/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_I732005/annotation1936869/range1936869/1
type: Range
persistent identity: range1936869
display id: range1936869
version: 1
top level: LacZ
direction: direction/forward
start: 3024
end: 3024
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1936872
display id: annotation1936872
version: 1
top level: LacZ
location: https://synbiohub.org/public/igem/BBa_I732005/annotation1936872/range1936872/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_I732005/annotation1936872/range1936872/1
type: Range
persistent identity: range1936872
display id: range1936872
version: 1
top level: LacZ
direction: direction/forward
start: 3073
end: 3075
orientation: inline

ChiPm
type: ComponentDefinition
persistent identity: BBa K564015
display id: BBa_K564015
version: 1
was generated by: iGEM to SBOL conversion
description: ChiP promoter and 5' UTR; RBS mutated AAGAGG-->AGGAGA
created: 2011-09-19T11:00:00Z
modified: 2015-05-08T01:12:42Z
mutable provenance: It was obtained by PCR from Escherichia coli W3110 genomic DNA.
owned by: james
owned by: myers
mutable description: ChiP encodes enterobacterial chitoporin required for uptake of chitin-derived oligosaccharides. This outer membrane porin is required for Salmonella and E.coli growth on chitooligosaccharides as a sole source of both carbon and nitrogen. It is only synthesized in the presence of these substrates. In nature it is regulated by sRNAa. We introduced the mutations in this sequence in the regulator binding site.
discontinued: false
dominant: false
experience: experience/None
group u list: _732_
m user id: 0
owner id: 8333
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: There are no geding considereations.
star: false
creator: Anna Lewinska
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: ChiP mutated
sequence annotation: changed binding site for ChiX
sequence: https://synbiohub.org/public/igem/BBa_K564015_sequence/1

ChiP mutated
type: SequenceAnnotation
persistent identity: annotation2135138
display id: annotation2135138
version: 1
top level: ChiPm
location: https://synbiohub.org/public/igem/BBa_K564015/annotation2135138/range2135138/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K564015/annotation2135138/range2135138/1
type: Range
persistent identity: range2135138
display id: range2135138
version: 1
top level: ChiPm
direction: direction/forward
start: 1
end: 599
orientation: inline

changed binding site for ChiX
type: SequenceAnnotation
persistent identity: annotation2135139
display id: annotation2135139
version: 1
top level: ChiPm
location: https://synbiohub.org/public/igem/BBa_K564015/annotation2135139/range2135139/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_K564015/annotation2135139/range2135139/1
type: Range
persistent identity: range2135139
display id: range2135139
version: 1
top level: ChiPm
direction: direction/forward
start: 578
end: 589
orientation: inline

https://synbiohub.org/public/igem/BBa_K564017_sequence/1
type: Sequence
persistent identity: BBa K564017 sequence
display id: BBa_K564017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gggcgaaagtaggcgagtaattttaagtttcgctatgccggatggggcgtttacgtcgcatccggcaaggaacagacaaacagtttcaaacgctaaattgcctgatgcgctacgcttatcaggcctacatgatctctgcaatatattgagtttgcgtgcttttgtaggccggataaggcgttcacgccgcatccggcaagaaacagcaaacaatccaaaacgccgcgttcagcggcgttttttctgcttttcttcgcgaattaattccgcttcgcaatttatccataaaataaatttaaaataacaaaacataattaaataaaatgtaaccgctttcatcttgctggaatttcacgcttttattcttctgcaagcctttcaaccgcaaacttaagccttgtaacaaaaatcatcaaaatatgtgcggttgctcatgttcttacattctggttacagaaagagattgataattcgcgtcgcgaaaaatagtctgttcctgtagtcagcgagacttttctcaacgctacttttttaatttttattttttcgctgttcacctttggtgcagcaatttatacgtcaaggagaattaacccatgtactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtgcgatcttcctgaggccgatactgtcgtcgtcccctcaaactggcagatgcacggttacgatgcgcccatctacaccaacgtgacctatcccattacggtcaatccgccgtttgttcccacggagaatccgacgggttgttactcgctcacatttaatgttgatgaaagctggctacaggaaggccagacgcgaattatttttgatggcgttaactcggcgtttcatctgtggtgcaacgggcgctgggtcggttacggccaggacagtcgtttgccgtctgaatttgacctgagcgcatttttacgcgccggagaaaaccgcctcgcggtgatggtgctgcgctggagtgacggcagttatctggaagatcaggatatgtggcggatgagcggcattttccgtgacgtctcgttgctgcataaaccgactacacaaatcagcgatttccatgttgccactcgctttaatgatgatttcagccgcgctgtactggaggctgaagttcagatgtgcggcgagttgcgtgactacctacgggtaacagtttctttatggcagggtgaaacgcaggtcgccagcggcaccgcgcctttcggcggtgaaattatcgatgagcgtggtggttatgccgatcgcgtcacactacgtctgaacgtcgaaaacccgaaactgtggagcgccgaaatcccgaatctctatcgtgcggtggttgaactgcacaccgccgacggcacgctgattgaagcagaagcctgcgatgtcggtttccgcgaggtgcggattgaaaatggtctgctgctgctgaacggcaagccgttgctgattcgaggcgttaaccgtcacgagcatcatcctctgcatggtcaggtcatggatgagcagacgatggtgcaggatatcctgctgatgaagcagaacaactttaacgccgtgcgctgttcgcattatccgaaccatccgctgtggtacacgctgtgcgaccgctacggcctgtatgtggtggatgaagccaatattgaaacccacggcatggtgccaatgaatcgtctgaccgatgatccgcgctggctaccggcgatgagcgaacgcgtaacgcgaatggtgcagcgcgatcgtaatcacccgagtgtgatcatctggtcgctggggaatgaatcaggccac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K564013_sequence/1
type: Sequence
persistent identity: BBa K564013 sequence
display id: BBa_K564013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gggcgaaagtaggcgagtaattttaagtttcgctatgccggatggggcgtttacgtcgcatccggcaaggaacagacaaacagtttcaaacgctaaattgcctgatgcgctacgcttatcaggcctacatgatctctgcaatatattgagtttgcgtgcttttgtaggccggataaggcgttcacgccgcatccggcaagaaacagcaaacaatccaaaacgccgcgttcagcggcgttttttctgcttttcttcgcgaattaattccgcttcgcaatttatccataaaataaatttaaaataacaaaacataattaaataaaatgtaaccgctttcatcttgctggaatttcacgcttttattcttctgcaagcctttcaaccgcaaacttaagccttgtaacaaaaatcatcaaaatatgtgcggttgctcatgttcttacattctggttacagaaagagattgataattcgcgtcgcgaaaaatagtctgttcctgtagtcagcgagacttttctcaacgctacttttttaatttttattttttcgctgttcacctttggtgcagcaatttatacgtcaaggagaattaacccatgtactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtgcgatcttcctgaggccgatactgtcgtcgtcccctcaaactggcagatgcacggttacgatgcgcccatctacaccaacgtgacctatcccattacggtcaatccgccgtttgttcccacggagaatccgacgggttgttactcgctcacatttaatgttgatgaaagctggctacaggaaggccagacgcgaattatttttgatggcgttaactcggcgtttcatctgtggtgcaacgggcgctgggtcggttacggccaggacagtcgtttgccgtctgaatttgacctgagcgcatttttacgcgccggagaaaaccgcctcgcggtgatggtgctgcgctggagtgacggcagttatctggaagatcaggatatgtggcggatgagcggcattttccgtgacgtctcgttgctgcataaaccgactacacaaatcagcgatttccatgttgccactcgctttaatgatgatttcagccgcgctgtactggaggctgaagttcagatgtgcggcgagttgcgtgactacctacgggtaacagtttctttatggcagggtgaaacgcaggtcgccagcggcaccgcgcctttcggcggtgaaattatcgatgagcgtggtggttatgccgatcgcgtcacactacgtctgaacgtcgaaaacccgaaactgtggagcgccgaaatcccgaatctctatcgtgcggtggttgaactgcacaccgccgacggcacgctgattgaagcagaagcctgcgatgtcggtttccgcgaggtgcggattgaaaatggtctgctgctgctgaacggcaagccgttgctgattcgaggcgttaaccgtcacgagcatcatcctctgcatggtcaggtcatggatgagcagacgatggtgcaggatatcctgctgatgaagcagaacaactttaacgccgtgcgctgttcgcattatccgaaccatccgctgtggtacacgctgtgcgaccgctacggcctgtatgtggtggatgaagccaatattgaaacccacggcatggtgccaatgaatcgtctgaccgatgatccgcgctggctaccggcgatgagcgaacgcgtaacgcgaatggtgcagcgcgatcgtaatcacccgagtgtgatcatctggtcgctggggaatgaatcaggccac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I732005_sequence/1
type: Sequence
persistent identity: BBa I732005 sequence
display id: BBa_I732005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtgcgatcttcctgaggccgatactgtcgtcgtcccctcaaactggcagatgcacggttacgatgcgcccatctacaccaacgtgacctatcccattacggtcaatccgccgtttgttcccacggagaatccgacgggttgttactcgctcacatttaatgttgatgaaagctggctacaggaaggccagacgcgaattatttttgatggcgttaactcggcgtttcatctgtggtgcaacgggcgctgggtcggttacggccaggacagtcgtttgccgtctgaatttgacctgagcgcatttttacgcgccggagaaaaccgcctcgcggtgatggtgctgcgctggagtgacggcagttatctggaagatcaggatatgtggcggatgagcggcattttccgtgacgtctcgttgctgcataaaccgactacacaaatcagcgatttccatgttgccactcgctttaatgatgatttcagccgcgctgtactggaggctgaagttcagatgtgcggcgagttgcgtgactacctacgggtaacagtttctttatggcagggtgaaacgcaggtcgccagcggcaccgcgcctttcggcggtgaaattatcgatgagcgtggtggttatgccgatcgcgtcacactacgtctgaacgtcgaaaacccgaaactgtggagcgccgaaatcccgaatctctatcgtgcggtggttgaactgcacaccgccgacggcacgctgattgaagcagaagcctgcgatgtcggtttccgcgaggtgcggattgaaaatggtctgctgctgctgaacggcaagccgttgctgattcgaggcgttaaccgtcacgagcatcatcctctgcatggtcaggtcatggatgagcagacgatggtgcaggatatcctgctgatgaagcagaacaactttaacgccgtgcgctgttcgcattatccgaaccatccgctgtggtacacgctgtgcgaccgctacggcctgtatgtggtggatgaagccaatattgaaacccacggcatggtgccaatgaatcgtctgaccgatgatccgcgctggctaccggcgatgagcgaacgcgtaacgcgaatggtgcagcgcgatcgtaatcacccgagtgtgatcatctggtcgctggggaatgaatcaggccacggcgctaatcacgacgcgctgtatcgctggatcaaatctgtcgatccttcccgcccggtgcagtatgaaggcggcggagccgacaccacggccaccgatattatttgcccgatgtacgcgcgcgtggatgaagaccagcccttcccggctgtgccgaaatggtccatcaaaaaatggctttcgctacctggagagacgcgcccgctgatcctttgcgaatacgcccacgcgatgggtaacagtcttggcggtttcgctaaatactggcaggcgtttcgtcagtatccccgtttacagggcggcttcgtctgggactgggtggatcagtcgctgattaaatatgatgaaaacggcaacccgtggtcggcttacggcggtgattttggcgatacgccgaacgatcgccagttctgtatgaacggtctggtctttgccgaccgcacgccgcatccagcgctgacggaagcaaaacaccagcagcagtttttccagttccgtttatccgggcaaaccatcgaagtgaccagcgaatacctgttccgtcatagcgataacgagctcctgcactggatggtggcgctggatggtaagccgctggcaagcgg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K564015_sequence/1
type: Sequence
persistent identity: BBa K564015 sequence
display id: BBa_K564015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gggcgaaagtaggcgagtaattttaagtttcgctatgccggatggggcgtttacgtcgcatccggcaaggaacagacaaacagtttcaaacgctaaattgcctgatgcgctacgcttatcaggcctacatgatctctgcaatatattgagtttgcgtgcttttgtaggccggataaggcgttcacgccgcatccggcaagaaacagcaaacaatccaaaacgccgcgttcagcggcgttttttctgcttttcttcgcgaattaattccgcttcgcaatttatccataaaataaatttaaaataacaaaacataattaaataaaatgtaaccgctttcatcttgctggaatttcacgcttttattcttctgcaagcctttcaaccgcaaacttaagccttgtaacaaaaatcatcaaaatatgtgcggttgctcatgttcttacattctggttacagaaagagattgataattcgcgtcgcgaaaaatagtctgttcctgtagtcagcgagacttttctcaacgctacttttttaatttttattttttcgctgttcacctttggtgcagcaatttatacgtcaaggagaattaacccatg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z