BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_K564000 1 BBa_K564000 ChiX 2011-09-17T11:00:00Z 2015-05-08T01:12:42Z ChiX is 84 bp long ChiX is 84 bp long false true _732_ 0 8342 9 Not in stock false ChiX is 84 bp long false Damian Rafal Plichta annotation2134903 1 chiX range2134903 1 1 84 BBa_K564014 1 Ptet-ChiX atC-->chiX 2011-09-19T11:00:00Z 2015-05-08T01:12:42Z The subparts were obtained from minigenes and fused together. This device is based on gene encoding small RNA (sRNA), which represses ChiP gene synthesis by pairing with a sequence in 5??? untranslated region (UTR) near ribosome binding site (RBS) of chiP mRNA. This leads to degradation of the targeted mRNA by enzymes called RNases.The repression occurs in the absence of the substrates (chitooligosaccharides) stimulating chiP gene transcription. It is fused with Ptet promoter. false true _732_ 0 8333 9 It's complicated false There are no design considerations false Anna Lewinska component2136523 1 BBa_K564021 component2136528 1 BBa_B0012 component2136526 1 BBa_B0010 component2136525 1 BBa_K564000 annotation2136523 1 BBa_K564021 range2136523 1 1 925 annotation2136526 1 BBa_B0010 range2136526 1 1026 1105 annotation2136528 1 BBa_B0012 range2136528 1 1114 1154 annotation2136525 1 BBa_K564000 range2136525 1 934 1017 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K564021 1 BBa_K564021 aTC regulated promoter module: aTC--PoPS 2011-09-20T11:00:00Z 2015-05-08T01:12:42Z DNA synthesis (integrated DNA technology) This part contains the tet regulated promoter R0040 as well as the wild-type tetR gene organized opposite one another to allow creation of an autonomous tet regulatable promoter module. Indirect evidence suggests it does and works as expected (induced by anhydrotetracycline). false false _732_ 0 5750 9 Not in stock false DNA was obtained by gene synthesis and a mutation within the -10 of the promoter driving expression of tetR makes it unsure whether it is produced or not by this part. false S??bastien Lemire annotation2136479 1 B0032 range2136479 1 769 781 annotation2136481 1 R0040 range2136481 1 864 918 annotation2136480 1 J23108 (-10 mutant) range2136480 1 790 825 annotation2136477 1 B0015 range2136477 1 1 123 annotation2136478 1 tetR range2136478 1 138 761 BBa_K564023 1 BBa_K564023 atC-->chiX 2011-09-20T11:00:00Z 2015-05-08T01:12:42Z DNA synthesis (integrated DNA technology) and ligation. This part contains a anhydrotetracycline regulatable promoter and the gene for chiX downstream of it. Upon aTC addition, it triggers expression of the sRNA chiX. true false _732_ 0 5750 9 Discontinued false None false S??bastien Lemire component2136508 1 BBa_B0015 component2136499 1 BBa_K564021 component2136501 1 BBa_K564000 annotation2136501 1 BBa_K564000 range2136501 1 934 1017 annotation2136499 1 BBa_K564021 range2136499 1 1 925 annotation2136508 1 BBa_B0015 range2136508 1 1026 1154 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K564014_sequence 1 tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtattaagacccactttcacatttaagttgtttttctaatccgcatatgatcaattcaaggccgaataagaaggctggctctgcaccttggtgatcaaataattcgatagcttgtcgtaataatggcggcatactatcagtagtaggtgtttccctttcttctttagcgacttgatgctcttgatcttccaatacgcaacctaaagtaaaatgccccacagcgctgagtgcatataatgcattctctagtgaaaaaccttgttggcataaaaaggctaattgattttcgagagtttcatactgtttttctgtaggccgtgtacctaaatgtacttttgctccatcgcgatgacttagtaaagcacatctaaaacttttagcgttattacgtaaaaaatcttgccagctttccccttctaaagggcaaaagtgagtatggtgcctatctaacatctcaatggctaaggcgtcgagcaaagcccgcttattttttacatgccaatacaatgtaggctgctctacacctagcttctgggcgagtttacgggttgttaaaccttcgattccgacctcattaagcagctctaatgcgctgttaatcactttacttttatctaatctgctcatctagtacctttcctgtgtgactctagtatctagcatttacataggactgaactagctgtcaatatgccatggatatgccggcatatctcgagatattatctttccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagattactagagacaccgtcgcttaaagtgacggcataataataaaaaaatgaaattcctctttgacgggccaatagcgatattggccattttttttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K564023_sequence 1 tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtattaagacccactttcacatttaagttgtttttctaatccgcatatgatcaattcaaggccgaataagaaggctggctctgcaccttggtgatcaaataattcgatagcttgtcgtaataatggcggcatactatcagtagtaggtgtttccctttcttctttagcgacttgatgctcttgatcttccaatacgcaacctaaagtaaaatgccccacagcgctgagtgcatataatgcattctctagtgaaaaaccttgttggcataaaaaggctaattgattttcgagagtttcatactgtttttctgtaggccgtgtacctaaatgtacttttgctccatcgcgatgacttagtaaagcacatctaaaacttttagcgttattacgtaaaaaatcttgccagctttccccttctaaagggcaaaagtgagtatggtgcctatctaacatctcaatggctaaggcgtcgagcaaagcccgcttattttttacatgccaatacaatgtaggctgctctacacctagcttctgggcgagtttacgggttgttaaaccttcgattccgacctcattaagcagctctaatgcgctgttaatcactttacttttatctaatctgctcatctagtacctttcctgtgtgactctagtatctagcatttacataggactgaactagctgtcaatatgccatggatatgccggcatatctcgagatattatctttccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagattactagagacaccgtcgcttaaagtgacggcataataataaaaaaatgaaattcctctttgacgggccaatagcgatattggccattttttttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K564000_sequence 1 acaccgtcgcttaaagtgacggcataataataaaaaaatgaaattcctctttgacgggccaatagcgatattggccattttttt BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K564021_sequence 1 tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtattaagacccactttcacatttaagttgtttttctaatccgcatatgatcaattcaaggccgaataagaaggctggctctgcaccttggtgatcaaataattcgatagcttgtcgtaataatggcggcatactatcagtagtaggtgtttccctttcttctttagcgacttgatgctcttgatcttccaatacgcaacctaaagtaaaatgccccacagcgctgagtgcatataatgcattctctagtgaaaaaccttgttggcataaaaaggctaattgattttcgagagtttcatactgtttttctgtaggccgtgtacctaaatgtacttttgctccatcgcgatgacttagtaaagcacatctaaaacttttagcgttattacgtaaaaaatcttgccagctttccccttctaaagggcaaaagtgagtatggtgcctatctaacatctcaatggctaaggcgtcgagcaaagcccgcttattttttacatgccaatacaatgtaggctgctctacacctagcttctgggcgagtttacgggttgttaaaccttcgattccgacctcattaagcagctctaatgcgctgttaatcactttacttttatctaatctgctcatctagtacctttcctgtgtgactctagtatctagcatttacataggactgaactagctgtcaatatgccatggatatgccggcatatctcgagatattatctttccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z