BBa_K566003 1 CFP opt CFP optimized for E. coli 2011-09-25T11:00:00Z 2015-05-08T01:12:42Z Synthesized DNA. Cyan fluorescent protein optimized for expression in E. coli. It was optimized using preferential codons, originally taken from BBa_E0022 false false _734_ 0 8650 9 Not in stock false Preferential codons were implemented for improving expression in E. coli. Analysis of illegal sites was made, they were found and removed. false Daniel Rodriguez annotation2141605 1 SsrA range2141605 1 718 758 annotation2141565 1 Optimized CFP range2141565 1 1 762 annotation2141604 1 Initial codon range2141604 1 1 3 annotation2141573 1 Stop range2141573 1 759 762 BBa_K566004 1 CFP CFP protein generator without transcription terminator 2011-09-25T11:00:00Z 2015-05-08T01:12:42Z Synthesized DNA. Protein generator of cyan fluorescent protein without transcription terminator. It may be negative regulated by Mnt repressor (BBa_C0072). false false _734_ 0 8650 9 Not in stock true Codon optimization for better expression in E. coli. Illegal sites were removed. false Daniel Rodriguez component2142652 1 BBa_R0073 component2142659 1 BBa_K566003 component2142654 1 BBa_B0034 annotation2142659 1 BBa_K566003 range2142659 1 80 841 annotation2142652 1 BBa_R0073 range2142652 1 1 67 annotation2142654 1 BBa_B0034 range2142654 1 68 79 BBa_R0073 1 Mnt Promoter (Mnt regulated) 2004-01-27T12:00:00Z 2015-05-08T01:14:15Z enterobacteriophage p22 Released HQ 2013 false false _1_ 0 24 7 In stock false unsure of where -35 site is true crackdots annotation318638 1 dimer right half range318638 1 32 38 annotation302669 1 mnt range302669 1 1 67 annotation318643 1 -10 range318643 1 47 52 annotation318636 1 dimer left half range318636 1 23 29 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K566004_sequence 1 ctcgaggtgagtgcacagtactaggtccacggtgacctagatctcctatagtgagtcgtattaatttaaagaggagaaaatggtttccaaaggcgaagaactgtttaccggtgttgttccgattctggttgaactggatggtgatgttaatggccacaaattttcagttagcggtgaaggcgaaggtgatgcaacctatggtaaactgaccctgaaatttatctgtaccaccggcaaactgccggttccgtggccgaccctggttaccaccctgacctggggtgttcagtgttttagccgttatccggatcatatgaaacagcacgattttttcaaaagcgcaatgccggaaggttatgttcaagaacgtaccatcttcttcaaagatgacggcaactataaaacccgtgccgaagttaaatttgaaggtgataccctggtgaatcgcattgaactgaaaggcatcgatttcaaagaggatggtaatatcctgggccacaaactggaatataattatatcagccacaacgtgtatatcaccgcagacaaacagaaaaatggcatcaaagccaactttaaaatccgccacaatattgaagatggcagcgttcagctggcagatcattatcagcagaataccccgattggtgatggtccggttctgctgccggataatcattatctgagcacccagagcgcactgagcaaagatccgaatgaaaaacgtgatcacatggtgctgctggaatttgttaccgcagcaggtattaccctgggtatggatgaactgtataaacgtccggcagccaatgatgaaaattatgcactggttgcctgataa BBa_R0073_sequence 1 ctcgaggtgagtgcacagtactaggtccacggtgacctagatctcctatagtgagtcgtattaattt BBa_B0034_sequence 1 aaagaggagaaa BBa_K566003_sequence 1 atggtttccaaaggcgaagaactgtttaccggtgttgttccgattctggttgaactggatggtgatgttaatggccacaaattttcagttagcggtgaaggcgaaggtgatgcaacctatggtaaactgaccctgaaatttatctgtaccaccggcaaactgccggttccgtggccgaccctggttaccaccctgacctggggtgttcagtgttttagccgttatccggatcatatgaaacagcacgattttttcaaaagcgcaatgccggaaggttatgttcaagaacgtaccatcttcttcaaagatgacggcaactataaaacccgtgccgaagttaaatttgaaggtgataccctggtgaatcgcattgaactgaaaggcatcgatttcaaagaggatggtaatatcctgggccacaaactggaatataattatatcagccacaacgtgtatatcaccgcagacaaacagaaaaatggcatcaaagccaactttaaaatccgccacaatattgaagatggcagcgttcagctggcagatcattatcagcagaataccccgattggtgatggtccggttctgctgccggataatcattatctgagcacccagagcgcactgagcaaagatccgaatgaaaaacgtgatcacatggtgctgctggaatttgttaccgcagcaggtattaccctgggtatggatgaactgtataaacgtccggcagccaatgatgaaaattatgcactggttgcctgataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z