BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7027
1
BBa_B0032
range7027
1
1
13
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation1710
1
RBS
range1710
1
7
10
BBa_K566012
1
cI opt
cI repressor from Lambda phage optimized for E. coli cI (+LVA)
2011-09-25T11:00:00Z
2015-05-08T01:12:43Z
Sequence was obtained form BioBrick BBa_C0052, it was optimized changing for preferential codons as possible and then synthesized.
cI repressor from Lambda phage optimized for E. coli cI (+LVA).
Preferential codons for better expression into E. coli.
Includes LVA tag.
false
false
_734_
0
8650
9
Not in stock
false
Search for illegal sites for classic BioBricks was performed, they were detected and eliminated.
false
Daniel Rodriguez
annotation2142581
1
cI
range2142581
1
1
711
annotation2142582
1
LVA
range2142582
1
712
744
BBa_K566016
1
BBa_K566016
OL-pTetMnt-cI
2011-09-25T11:00:00Z
2015-05-08T01:12:43Z
Synthesized DNA.
Induction system for Biphasic Switch.
It responds to negative repression from TetR or cI repressor.
false
false
_734_
0
8650
9
Not in stock
false
Coding sequences were optimized with preferential codons. Search for illegal sites was performed, sites were localized and deleted.
false
Daniel Rodriguez
component2142685
1
BBa_K566000
component2142690
1
BBa_K091105
component2142692
1
BBa_B0032
component2142696
1
BBa_K566012
annotation2142685
1
BBa_K566000
range2142685
1
1
129
annotation2142692
1
BBa_B0032
range2142692
1
228
240
annotation2142690
1
BBa_K091105
range2142690
1
130
227
annotation2142696
1
BBa_K566012
range2142696
1
241
990
BBa_K566000
1
OL
OL region from Lambda
2011-09-25T11:00:00Z
2015-05-08T01:12:42Z
It comes directly from genomic DNA of Lambda phage. It was extracted by PCR as recommended by the Registry.
OL region form Lambda phage. It is used to allow a construction to be controlled dually by cI protein. cI protein may interact with pRM promoter and OL region in order to form an octameric structure which permits, first the activation of transcription from pRM promoter and then effectively repression from it as well.
false
false
_734_
0
8650
9
Not in stock
false
Analysis of illegal restriction sites was performed, but it does not have them originally.
false
Daniel Rodriguez, Eduardo Almeyda, Porfirio Quintero
annotation2146174
1
OL
range2146174
1
1
129
BBa_K091105
1
BBa_K091105
pTet/Mnt Hybrid Promoter
2008-06-18T11:00:00Z
2015-05-08T01:08:37Z
This part comes from the Mnt promoter and the TetR promoter
This promoter is a modified version of the Mnt promoter that is also responsive to TetR. The promoter should be repressed by Mnt repressor. It should also be repressed by TetR, and in the absence of Mnt repressor, should be induced by aTc.
false
false
_191_
0
3080
9
It's complicated
true
Mnt repressor binds as a tetramer to two half-operator sites. Introduction of TetR binding sites between the Mnt promoter -10 sequence and the start site for transcription should allow for repression by TetR. Accordingly, the hybrid promoter was designed by 1) Remove all bases of mnt promoter 3??? to -10: gagtcgtattaattt will be replaced by tccctatcagtgata, and 2) truncate the second half of ptet -10 and mutate -35 so that it function to bind tetR (replace ttgaca with actgta), but is not a promoter, and 3) place the tetR1 and tetR2 binding sites 3??? to truncated mnt -10 sequence.
true
Robert Cool
annotation1963740
1
tetR1
range1963740
1
53
71
annotation1963739
1
-10
range1963739
1
47
52
annotation1963742
1
Mnt
range1963742
1
1
52
annotation1963741
1
tetR2
range1963741
1
78
98
BBa_K566016_sequence
1
cagatctctcacctaccaaacaatgcccccctgcaaaaaataaattcatataaaaaacatacagataaccatctgcggtgataaattatctctggcggtgttgacataaataccactggcggtgatactctcgaggtgagtgcacagtactaggtccacggtgacctagatctcctatagttccctatcagtgatagagaactgtaatccctatcagtgatagagattcacacaggaaagatgagcaccaaaaaaaaaccgctgacccaagaacagctggaagatgcacgtcgtctgaaagcaatctacgagaaaaaaaaaaatgaactgggcctgagccaagaaagcgttgcagataaaatgggtatgggtcagagcggtgttggtgcactgtttaatggtattaatgcactgaatgcctataatgcagcactgctggcaaaaattctgaaagttagcgttgaagagttcagcccgagcattgcacgtgaaatctatgaaatgtatgaagccgttagcatgcagccgagcctgcgtagcgaatatgaatatccggtttttagccatgttcaggcaggtatgtttagtccggaactgcgtacctttaccaaaggtgatgcagaacgttgggttagcacaaccaaaaaagcaagcgatagcgcattttggctggaagttgaaggtaatagcatgaccgcaccgaccggtagcaaaccgagctttccggatggtatgctgattctggttgatccggaacaggcagttgaaccgggtgatttttgtattgcacgtctgggtggtgatgagttcaccttcaaaaaactgattcgtgatagcggtcaggtttttctgcaaccgctgaatccgcagtatccgatgattccgtgtaatgaaagctgtagcgttgtgggtaaagttattgcaagccagtggcctgaagaaacctttggtgcagcaaatgatgaaaattatgcactggtggcctgataa
BBa_B0032_sequence
1
tcacacaggaaag
BBa_K566012_sequence
1
atgagcaccaaaaaaaaaccgctgacccaagaacagctggaagatgcacgtcgtctgaaagcaatctacgagaaaaaaaaaaatgaactgggcctgagccaagaaagcgttgcagataaaatgggtatgggtcagagcggtgttggtgcactgtttaatggtattaatgcactgaatgcctataatgcagcactgctggcaaaaattctgaaagttagcgttgaagagttcagcccgagcattgcacgtgaaatctatgaaatgtatgaagccgttagcatgcagccgagcctgcgtagcgaatatgaatatccggtttttagccatgttcaggcaggtatgtttagtccggaactgcgtacctttaccaaaggtgatgcagaacgttgggttagcacaaccaaaaaagcaagcgatagcgcattttggctggaagttgaaggtaatagcatgaccgcaccgaccggtagcaaaccgagctttccggatggtatgctgattctggttgatccggaacaggcagttgaaccgggtgatttttgtattgcacgtctgggtggtgatgagttcaccttcaaaaaactgattcgtgatagcggtcaggtttttctgcaaccgctgaatccgcagtatccgatgattccgtgtaatgaaagctgtagcgttgtgggtaaagttattgcaagccagtggcctgaagaaacctttggtgcagcaaatgatgaaaattatgcactggtggcctgataa
BBa_K566000_sequence
1
cagatctctcacctaccaaacaatgcccccctgcaaaaaataaattcatataaaaaacatacagataaccatctgcggtgataaattatctctggcggtgttgacataaataccactggcggtgatact
BBa_K091105_sequence
1
ctcgaggtgagtgcacagtactaggtccacggtgacctagatctcctatagttccctatcagtgatagagaactgtaatccctatcagtgatagagat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z