BBa_K566018 1 BBa_K566018 E. coli MXRed 2011-09-25T11:00:00Z 2015-05-08T01:12:43Z E. coli JT2 strain Derived from Dr. Jeff Tabor JT2 E. coli strain, this part is a built-in red-light induction system in E. coli made through chromosome insertion. Avoiding the need of any extra-chromosomal DNA when light-inducing gene expression offering several advantages to the researcher. It is proposed as a photo-chassis that could make useful tools in this field of light induction. Integration was performed for the genes pcyA, ho1, Cph8 and a Mnt working as follows: Genes ho1 and pcyA are responsible for the chromophore synthesis. Cph8 codes for the chimaeric red-light receptor. These three genes are constitutively expressed. Mnt repressor is expressed from pOmpC promoter, which stops being induced in presence of red-light. It is therefore used as a NOT-gate to regulate expression from pMnt. true false _734_ 0 8650 9 Discontinued false Integration protocol of Dr. Kuhlmann false Daniel Rodriguez annotation2142720 1 BBa_C0072 range2142720 1 1 288 annotation2142717 1 mnt range2142717 1 1 249 annotation2142721 1 barcode range2142721 1 289 313 annotation2142718 1 T range2142718 1 95 97 annotation2142719 1 LVA range2142719 1 250 282 igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z