LOCUS BBa_K566019 4916 bp DNA linear 22-Nov-2024 DEFINITION E. coli MxRed ACCESSION BBa_K566019 VERSION BBa_K566019.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 RBS 44..58 /label=RBS-1\Strong RBS 51..55 /label=RBS misc_feature 44..58 /label=BBa_B0030 RBS 44..58 /label=BBa_B0030 misc_feature 1..58 /label=BBa_K081005 CDS 65..2299 /label=Cph8 CDS 65..2299 /label=BBa_K566025 stem_loop 2319..2362 /label=stem_loop misc_feature 2308..2387 /label=BBa_B0010 terminator 2308..2387 /label=BBa_B0010 stem_loop 2403..2422 /label=T7 TE misc_feature 2429..2429 /label=stop polyA_site 2423..2436 /label=polya misc_feature 2396..2436 /label=BBa_B0012 terminator 2396..2436 /label=BBa_B0012 terminator 2308..2436 /label=BBa_B0015 misc_feature 1..2436 /label=BBa_K566026 misc_feature 2457..2474 /label=C1 OmpR misc_feature 2478..2495 /label=C2 OmpR misc_feature 2498..2515 /label=C3 OmpR promoter 2519..2524 /label=-35 promoter 2542..2547 /label=-10 promoter 2445..2552 /label=BBa_R0082 misc_feature 2565..2568 /label=conserved RBS 2561..2572 /label=BBa_B0034 CDS 2579..2827 /label=mnt misc_feature 2673..2675 /label=T misc_feature 2828..2860 /label=LVA misc_feature 2867..2891 /label=Help:Barcodes CDS 2579..2891 /label=BBa_C0072 stem_loop 2911..2954 /label=stem_loop misc_feature 2900..2979 /label=BBa_B0010 terminator 2900..2979 /label=BBa_B0010 stem_loop 2995..3014 /label=T7 TE misc_feature 3021..3021 /label=stop polyA_site 3015..3028 /label=polya misc_feature 2988..3028 /label=BBa_B0012 terminator 2988..3028 /label=BBa_B0012 terminator 2900..3028 /label=BBa_B0015 misc_feature 2445..3028 /label=BBa_K566020 promoter 3037..3071 /label=BBa_J23100 RBS 3080..3094 /label=RBS-1\Strong RBS 3087..3091 /label=RBS misc_feature 3080..3094 /label=BBa_B0030 RBS 3080..3094 /label=BBa_B0030 misc_feature 3037..3094 /label=BBa_K081005 CDS 3101..3847 /label=pcyA CDS 3101..3847 /label=BBa_K566021 stem_loop 3867..3910 /label=stem_loop misc_feature 3856..3935 /label=BBa_B0010 terminator 3856..3935 /label=BBa_B0010 stem_loop 3951..3970 /label=T7 TE misc_feature 3977..3977 /label=stop polyA_site 3971..3984 /label=polya misc_feature 3944..3984 /label=BBa_B0012 terminator 3944..3984 /label=BBa_B0012 terminator 3856..3984 /label=BBa_B0015 misc_feature 3037..3984 /label=BBa_K566023 promoter 3993..4027 /label=BBa_J23100 RBS 4036..4050 /label=RBS-1\Strong RBS 4043..4047 /label=RBS misc_feature 4036..4050 /label=BBa_B0030 RBS 4036..4050 /label=BBa_B0030 misc_feature 3993..4050 /label=BBa_K081005 CDS 4057..4779 /label=ho1 CDS 4057..4779 /label=BBa_K566022 stem_loop 4799..4842 /label=stem_loop misc_feature 4788..4867 /label=BBa_B0010 terminator 4788..4867 /label=BBa_B0010 stem_loop 4883..4902 /label=T7 TE misc_feature 4909..4909 /label=stop polyA_site 4903..4916 /label=polya misc_feature 4876..4916 /label=BBa_B0012 terminator 4876..4916 /label=BBa_B0012 terminator 4788..4916 /label=BBa_B0015 misc_feature 3993..4916 /label=BBa_K566024 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagattaaag aggagaaata 61 ctagatggcc accaccgtac aactcagcga ccaatccctc cgtcagctag aaaccctcgc 121 catccacacc gcccacctga ttcagcccca cggtttagtg gtggtcctgc aggaaccaga 181 cctcaccatc agccaaatta gcgccaactg caccggcatt ttagggcgat cgccagagga 241 tttgttgggc agaaccctag gggaagtgtt tgatagcttt cagattgatc ccatccagag 301 tcgcctaacg gccggacaaa tcagcagcct caaccccagt aaactttggg cgcgggtcat 361 gggggacgac tttgtcattt ttgacggggt ttttcatcgc aacagtgacg gtttattggt 421 atgtgaacta gagccagcct acacttccga taatctgccc ttcctcggtt tttatcacat 481 ggccaacgct gccctgaatc ggttgcgcca acaagctaat ctacgggatt tctacgatgt 541 tattgtcgaa gaagtccgcc gtatgactgg ctttgaccgg gtgatgctat accgctttga 601 tgaaaataac cacggtgatg tcattgccga agataaacgg gatgatatgg aaccctattt 661 gggcctgcac tatcccgaat cggatattcc ccaacccgcc cgtcggctat ttatccacaa 721 ccccattcga gtaattcccg atgtttatgg tgtggcggtg cccctgaccc cagcggttaa 781 ccccagcacc aaccgagcgg tggatttaac agaatccatt ctgcgcagtg cgtaccattg 841 ccacttgacc tatctgaaaa atatgggggt aggagcgtct ttaaccattt ccctaattaa 901 ggacggccat ctctgggggc tcattgcctg ccaccatcaa acccccaaag taattccctt 961 tgaactgcgt aaagcctgcg aattttttgg tcgggtggtg tttagcaaca tttccgccca 1021 ggaagatacg gaaaccttcg attaccgggt gcagttggcg gagcatgaag cggttttatt 1081 ggacaaaatg accacggcgg cggattttgt cgaaggatta actaatcatc ccgatcgcct 1141 gttgggatta acgggctccc agggggcggc catttgcttt ggggaaaaat tgattttagt 1201 aggggaaacc ccggacgaga aagcagtgca atatttactg caatggttgg agaatcggga 1261 agtgcaagac gttttcttca cctcttccct ctcacaaatt tatcctgatg cagtgaattt 1321 taaatccgtg gccagtggct tattggccat tcccattgcc cgtcacaact ttttgctctg 1381 gtttcgccct gaagtgttgc aaacggttaa ttggggcggt gacccaaatc atgcttacga 1441 agctacccag gaagacggta aaatcgagct ccatccccgc caatcctttg acctctggaa 1501 agaaattgtc cgactccaat ctttgccctg gcaatcggtg gaaatccaaa gtgccctggc 1561 cctgaaaaag gcgatcgtca acctcatttt gcgccaggca gaagaattgc atatggcggc 1621 tggtgttaag caactggcgg atgaccgcac gctgctgatg gcgggggtaa gtcacgactt 1681 gcgcacgccg ctgacgcgta ttcgcctggc gactgagatg atgagcgagc aggatggcta 1741 tctggcagaa tcgatcaata aagatatcga agagtgcaac gccatcattg agcagtttat 1801 cgactacctg cgcaccgggc aggagatgcc gatggaaatg gcggatctta atgcagtact 1861 cggtgaggtg attgctgccg aaagtggcta tgagcgggaa attgaaaccg cgctttaccc 1921 cggcagcatt gaagtgaaaa tgcacccgct gtcgatcaaa cgcgcggtgg cgaatatggt 1981 ggtcaacgcc gcccgttatg gcaatggctg gatcaaagtc agcagcggaa cggagccgaa 2041 tcgcgcctgg ttccaggtgg aagatgacgg tccgggaatt gcgccggaac aacgtaagca 2101 cctgttccag ccgtttgtcc gcggcgacag tgcgcgcacc attagcggca cgggattagg 2161 gctggcaatt gtgcagcgta tcgtggataa ccataacggg atgctggagc ttggcaccag 2221 cgagcggggc gggctttcca ttcgcgcctg gctgccagtg ccggtaacgc gggcgcaggg 2281 catgacaaaa gaagggtaat actagagcca ggcatcaaat aaaacgaaag gctcagtcga 2341 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 2401 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtccctt gcatttacat 2461 tttgaaacat ctatagcgat aaatgaaaca tcttaaaagt tttagtatca tattcgtgtt 2521 ggattattct gcatttttgg ggagaatgga cttactagag aaagaggaga aatactagat 2581 ggcccgggat gatcctcact tcaattttcg tatgccaatg gaagtaagag agaaattgaa 2641 atttagagca gaggcaaacg gacggagcat gaactctgag cttttgcaaa tcgtacaaga 2701 tgccctaagc aaaccgtcac cagtcactgg gtaccgcaat gatgcggaac gactcgccga 2761 tgagcagagc gagttagtga agaagatggt cttcgataca ctgaaggatc tttataaaaa 2821 aaccaccgct gcaaacgacg aaaactacgc tttagtagct taataaccct gatagtgcta 2881 gtgtagatcc ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 2941 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 3001 ccttcgggtg ggcctttctg cgtttatata ctagagttga cggctagctc agtcctaggt 3061 acagtgctag ctactagaga ttaaagagga gaaatactag atggccgtca ctgatttaag 3121 tttgaccaat tcttccctga tgcctacgtt gaacccgatg attcaacagt tggccctggc 3181 gatcgccgct agttggcaaa gtttacccct caagccctat caattgccgg aggatttggg 3241 ctacgtagaa ggccgcctgg aaggggaaaa gttagtgatt gaaaatcggt gctaccaaac 3301 gccccagttt cgcaaaatgc atttggagtt ggccaaggtg ggcaaagggt tggatattct 3361 ccactgtgta atgtttcctg agcctttata cggtctacct ttgtttggct gtgacattgt 3421 ggccggcccc ggtggagtaa gtgcggctat tgcggatcta tcccccaccc aaagcgatcg 3481 ccaattgccc gcagcgtacc aaaaatcatt ggcagagcta ggccagccag aatttgagca 3541 acaacgggaa ttgcccccct ggggagaaat attttctgaa tattgtttat tcatccgtcc 3601 cagcaatgtc actgaagaag aaagatttgt acaaagggta gtggactttt tgcaaattca 3661 ttgtcaccaa tccatcgttg ccgaaccctt gtctgaagct caaactttgg agcaccgtca 3721 ggggcaaatt cattactgcc aacaacaaca gaaaaatgat aaaacccgtc gggtactgga 3781 aaaagctttt ggggaagctt gggcggaacg gtatatgagc caagtcttat ttgatgttat 3841 ccaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 3901 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 3961 cgggtgggcc tttctgcgtt tatatactag agttgacggc tagctcagtc ctaggtacag 4021 tgctagctac tagagattaa agaggagaaa tactagatga gtgtcaactt agcttcccag 4081 ttgcgggaag ggacgaaaaa atcccactcc atggcggaga acgtcggctt tgtcaaatgc 4141 ttcctcaagg gcgttgtcga gaaaaattcc taccgtaagc tggttggcaa tctctacttt 4201 gtctacagtg ccatggaaga ggaaatggca aaatttaagg accatcccat cctcagccac 4261 atttacttcc ccgaactcaa ccgcaaacaa agcctagagc aagacctgca attctattac 4321 ggctccaact ggcggcaaga agtgaaaatt tctgccgctg gccaagccta tgtggaccga 4381 gtccggcaag tggccgctac ggcccctgaa ttgttggtgg cccattccta cacccgttac 4441 ctgggggatc tttccggcgg tcaaattctc aagaaaattg cccaaaatgc catgaatctc 4501 cacgatggtg gcacagcttt ctatgaattt gccgacattg atgacgaaaa ggcttttaaa 4561 aatacctacc gtcaagctat gaatgatctg cccattgacc aagccaccgc cgaacggatt 4621 gtggatgaag ccaatgacgc ctttgccatg aacatgaaaa tgttcaacga acttgaaggc 4681 aacctgatca aggcgatcgg cattatggtg ttcaacagcc tcacccgtcg ccgcagtcaa 4741 ggcagcaccg aagttggcct cgccacctcc gaaggctagt actagagcca ggcatcaaat 4801 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 4861 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata //