##gff-version 3
##sequence-region BBa_K566019 1 4916
BBa_K566019	.	engineered_region	2445	3028	.	+	0	ID=BBa_K566020;Name=BBa_K566020
BBa_K566019	.	terminator	2900	3028	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K566020
BBa_K566019	.	terminator	2900	2979	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K566019	.	engineered_region	2900	2979	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K566019	.	stem_loop	2911	2954	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K566019	.	terminator	2988	3028	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K566019	.	stop_codon	3021	3021	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K566019	.	polyA_site	3015	3028	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K566019	.	stem_loop	2995	3014	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K566019	.	engineered_region	2988	3028	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K566019	.	ribosome_entry_site	2561	2572	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K566020
BBa_K566019	.	conserved_region	2565	2568	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K566019	.	CDS	2579	2891	.	+	0	ID=BBa_C0072;Name=BBa_C0072;Parent=BBa_K566020
BBa_K566019	.	tag	2828	2860	.	+	0	ID=annotation308503;Name=LVA;Parent=BBa_C0072
BBa_K566019	.	point_mutation	2673	2675	.	+	0	ID=annotation308502;Name=T;Parent=BBa_C0072
BBa_K566019	.	CDS	2579	2827	.	+	0	ID=annotation308499;Name=mnt;Parent=BBa_C0072
BBa_K566019	.	engineered_tag	2867	2891	.	+	0	ID=annotation2214010;Name=Help:Barcodes;Parent=BBa_C0072
BBa_K566019	.	promoter	2445	2552	.	+	0	ID=BBa_R0082;Name=BBa_R0082;Parent=BBa_K566020
BBa_K566019	.	non_covalent_binding_site	2478	2495	.	+	0	ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082
BBa_K566019	.	non_covalent_binding_site	2498	2515	.	+	0	ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082
BBa_K566019	.	promoter	2542	2547	.	+	0	ID=annotation301167;Name=-10;Parent=BBa_R0082
BBa_K566019	.	promoter	2519	2524	.	+	0	ID=annotation301166;Name=-35;Parent=BBa_R0082
BBa_K566019	.	non_covalent_binding_site	2457	2474	.	+	0	ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082
BBa_K566019	.	engineered_region	3037	3984	.	+	0	ID=BBa_K566023;Name=BBa_K566023
BBa_K566019	.	engineered_region	3037	3094	.	+	0	ID=BBa_K081005;Name=BBa_K081005;Parent=BBa_K566023
BBa_K566019	.	ribosome_entry_site	3080	3094	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081005
BBa_K566019	.	ribosome_entry_site	3087	3091	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K566019	.	engineered_region	3080	3094	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K566019	.	ribosome_entry_site	3080	3094	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K566019	.	promoter	3037	3071	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K081005
BBa_K566019	.	CDS	3101	3847	.	+	0	ID=BBa_K566021;Name=BBa_K566021;Parent=BBa_K566023
BBa_K566019	.	CDS	3101	3847	.	+	0	ID=annotation2142741;Name=pcyA;Parent=BBa_K566021
BBa_K566019	.	terminator	3856	3984	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K566023
BBa_K566019	.	terminator	3856	3935	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K566019	.	engineered_region	3856	3935	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K566019	.	stem_loop	3867	3910	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K566019	.	terminator	3944	3984	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K566019	.	stop_codon	3977	3977	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K566019	.	polyA_site	3971	3984	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K566019	.	stem_loop	3951	3970	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K566019	.	engineered_region	3944	3984	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K566019	.	engineered_region	1	2436	.	+	0	ID=BBa_K566026;Name=BBa_K566026
BBa_K566019	.	engineered_region	1	58	.	+	0	ID=BBa_K081005;Name=BBa_K081005;Parent=BBa_K566026
BBa_K566019	.	ribosome_entry_site	44	58	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081005
BBa_K566019	.	ribosome_entry_site	51	55	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K566019	.	engineered_region	44	58	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K566019	.	ribosome_entry_site	44	58	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K566019	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K081005
BBa_K566019	.	terminator	2308	2436	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K566026
BBa_K566019	.	terminator	2308	2387	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K566019	.	engineered_region	2308	2387	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K566019	.	stem_loop	2319	2362	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K566019	.	terminator	2396	2436	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K566019	.	stop_codon	2429	2429	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K566019	.	polyA_site	2423	2436	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K566019	.	stem_loop	2403	2422	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K566019	.	engineered_region	2396	2436	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K566019	.	CDS	65	2299	.	+	0	ID=BBa_K566025;Name=BBa_K566025;Parent=BBa_K566026
BBa_K566019	.	CDS	65	2299	.	+	0	ID=annotation2142823;Name=Cph8;Parent=BBa_K566025
BBa_K566019	.	engineered_region	3993	4916	.	+	0	ID=BBa_K566024;Name=BBa_K566024
BBa_K566019	.	terminator	4788	4916	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K566024
BBa_K566019	.	terminator	4788	4867	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K566019	.	engineered_region	4788	4867	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K566019	.	stem_loop	4799	4842	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K566019	.	terminator	4876	4916	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K566019	.	stop_codon	4909	4909	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K566019	.	polyA_site	4903	4916	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K566019	.	stem_loop	4883	4902	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K566019	.	engineered_region	4876	4916	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K566019	.	CDS	4057	4779	.	+	0	ID=BBa_K566022;Name=BBa_K566022;Parent=BBa_K566024
BBa_K566019	.	CDS	4057	4779	.	+	0	ID=annotation2142743;Name=ho1;Parent=BBa_K566022
BBa_K566019	.	engineered_region	3993	4050	.	+	0	ID=BBa_K081005;Name=BBa_K081005;Parent=BBa_K566024
BBa_K566019	.	ribosome_entry_site	4036	4050	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081005
BBa_K566019	.	ribosome_entry_site	4043	4047	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K566019	.	engineered_region	4036	4050	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K566019	.	ribosome_entry_site	4036	4050	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K566019	.	promoter	3993	4027	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K081005
>BBa_K566019
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatggccaccaccgta
caactcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccgcccacctgattcagccccacggtttag
tggtggtcctgcaggaaccagacctcaccatcagccaaattagcgccaactgcaccggcattttagggcgatcgccaga
ggatttgttgggcagaaccctaggggaagtgtttgatagctttcagattgatcccatccagagtcgcctaacggccgga
caaatcagcagcctcaaccccagtaaactttgggcgcgggtcatgggggacgactttgtcatttttgacggggtttttc
atcgcaacagtgacggtttattggtatgtgaactagagccagcctacacttccgataatctgcccttcctcggttttta
tcacatggccaacgctgccctgaatcggttgcgccaacaagctaatctacgggatttctacgatgttattgtcgaagaa
gtccgccgtatgactggctttgaccgggtgatgctataccgctttgatgaaaataaccacggtgatgtcattgccgaag
ataaacgggatgatatggaaccctatttgggcctgcactatcccgaatcggatattccccaacccgcccgtcggctatt
tatccacaaccccattcgagtaattcccgatgtttatggtgtggcggtgcccctgaccccagcggttaaccccagcacc
aaccgagcggtggatttaacagaatccattctgcgcagtgcgtaccattgccacttgacctatctgaaaaatatggggg
taggagcgtctttaaccatttccctaattaaggacggccatctctgggggctcattgcctgccaccatcaaacccccaa
agtaattccctttgaactgcgtaaagcctgcgaattttttggtcgggtggtgtttagcaacatttccgcccaggaagat
acggaaaccttcgattaccgggtgcagttggcggagcatgaagcggttttattggacaaaatgaccacggcggcggatt
ttgtcgaaggattaactaatcatcccgatcgcctgttgggattaacgggctcccagggggcggccatttgctttgggga
aaaattgattttagtaggggaaaccccggacgagaaagcagtgcaatatttactgcaatggttggagaatcgggaagtg
caagacgttttcttcacctcttccctctcacaaatttatcctgatgcagtgaattttaaatccgtggccagtggcttat
tggccattcccattgcccgtcacaactttttgctctggtttcgccctgaagtgttgcaaacggttaattggggcggtga
cccaaatcatgcttacgaagctacccaggaagacggtaaaatcgagctccatccccgccaatcctttgacctctggaaa
gaaattgtccgactccaatctttgccctggcaatcggtggaaatccaaagtgccctggccctgaaaaaggcgatcgtca
acctcattttgcgccaggcagaagaattgcatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgat
ggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggc
tatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccg
ggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcg
ggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaat
atggtggtcaacgccgcccgttatggcaatggctggatcaaagtcagcagcggaacggagccgaatcgcgcctggttcc
aggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgc
gcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagctt
ggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcatgacaaaag
aagggtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt
tgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccct
tgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattatt
ctgcatttttggggagaatggacttactagagaaagaggagaaatactagatggcccgggatgatcctcacttcaattt
tcgtatgccaatggaagtaagagagaaattgaaatttagagcagaggcaaacggacggagcatgaactctgagcttttg
caaatcgtacaagatgccctaagcaaaccgtcaccagtcactgggtaccgcaatgatgcggaacgactcgccgatgagc
agagcgagttagtgaagaagatggtcttcgatacactgaaggatctttataaaaaaaccaccgctgcaaacgacgaaaa
ctacgctttagtagcttaataaccctgatagtgctagtgtagatccctactagagccaggcatcaaataaaacgaaagg
ctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
ttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtcctaggtacagtgctagctactagagat
taaagaggagaaatactagatggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatg
attcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgg
gctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaat
gcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtcta
cctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcg
atcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcc
cccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaa
agggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagc
accgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttgg
ggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataatactagagccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtcctaggtacagtgctagcta
ctagagattaaagaggagaaatactagatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccact
ccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttgg
caatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttac
ttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtga
aaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggccca
ttcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccac
gatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatga
atgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaat
gttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaa
ggcagcaccgaagttggcctcgccacctccgaaggctagtactagagccaggcatcaaataaaacgaaaggctcagtcg
aaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtg
ggcctttctgcgtttata