BBa_K566019
type: ComponentDefinition
persistent identity: BBa K566019
display id: BBa_K566019
version: 1
was generated by: iGEM to SBOL conversion
description: E. coli MxRed
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:12:43Z
mutable provenance: E. coli JT2 strain
owned by: james
owned by: myers
mutable description: Derived from Dr. Jeff Tabor JT2 E. coli strain, this part is a built-in red-light induction system in E. coli made through chromosome insertion. Avoiding the need of any extra-chromosomal DNA when light-inducing gene expression offering several advantages to the researcher. It is proposed as a photo-chassis that could make useful tools in this field of light induction. Integration was performed for the genes pcyA, ho1, Cph8 and a Mnt working as follows: Genes ho1 and pcyA are responsible for the chromophore synthesis. Cph8 codes for the chimaeric red-light receptor. These three genes are constitutively expressed. Mnt repressor is expressed from pOmpC promoter, which stops being induced in presence of red-light. It is therefore used as a NOT-gate to regulate expression from pMnt.
discontinued: false
dominant: false
experience: experience/None
group u list: _734_
m user id: 0
owner id: 8650
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Integration performed through protocol of Dr. Kuhlmann
star: false
creator: Daniel Rodriguez
type: DnaRegion
role: SO:0000340
role: partType/Cell
component: BBa_K566024
component: BBa_K566023
component: BBa_K566020
component: BBa_K566026
sequence annotation: BBa_K566026
sequence annotation: BBa_K566020
sequence annotation: BBa_K566023
sequence annotation: BBa_K566024
sequence: https://synbiohub.org/public/igem/BBa_K566019_sequence/1

BBa_K566024
type: Component
persistent identity: component2321066
display id: component2321066
version: 1
top level: BBa_K566019
definition: BBa_K566024
access: public

BBa_K566023
type: Component
persistent identity: component2321051
display id: component2321051
version: 1
top level: BBa_K566019
definition: BBa_K566023
access: public

BBa_K566020
type: Component
persistent identity: component2321036
display id: component2321036
version: 1
top level: BBa_K566019
definition: BBa_K566020
access: public

BBa_K566026
type: Component
persistent identity: component2321015
display id: component2321015
version: 1
top level: BBa_K566019
definition: Cph8 gene
access: public

BBa_K566026
type: SequenceAnnotation
persistent identity: annotation2321015
display id: annotation2321015
version: 1
top level: BBa_K566019
location: https://synbiohub.org/public/igem/BBa_K566019/annotation2321015/range2321015/1
component: BBa_K566026

https://synbiohub.org/public/igem/BBa_K566019/annotation2321015/range2321015/1
type: Range
persistent identity: range2321015
display id: range2321015
version: 1
top level: BBa_K566019
direction: direction/reverse
start: 1
end: 2436
orientation: inline

BBa_K566020
type: SequenceAnnotation
persistent identity: annotation2321036
display id: annotation2321036
version: 1
top level: BBa_K566019
location: https://synbiohub.org/public/igem/BBa_K566019/annotation2321036/range2321036/1
component: BBa_K566020

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type: Range
persistent identity: range2321036
display id: range2321036
version: 1
top level: BBa_K566019
direction: direction/reverse
start: 2445
end: 3028
orientation: inline

BBa_K566023
type: SequenceAnnotation
persistent identity: annotation2321051
display id: annotation2321051
version: 1
top level: BBa_K566019
location: https://synbiohub.org/public/igem/BBa_K566019/annotation2321051/range2321051/1
component: BBa_K566023

https://synbiohub.org/public/igem/BBa_K566019/annotation2321051/range2321051/1
type: Range
persistent identity: range2321051
display id: range2321051
version: 1
top level: BBa_K566019
direction: direction/reverse
start: 3037
end: 3984
orientation: inline

BBa_K566024
type: SequenceAnnotation
persistent identity: annotation2321066
display id: annotation2321066
version: 1
top level: BBa_K566019
location: https://synbiohub.org/public/igem/BBa_K566019/annotation2321066/range2321066/1
component: BBa_K566024

https://synbiohub.org/public/igem/BBa_K566019/annotation2321066/range2321066/1
type: Range
persistent identity: range2321066
display id: range2321066
version: 1
top level: BBa_K566019
direction: direction/reverse
start: 3993
end: 4916
orientation: inline

BBa_K566020
type: ComponentDefinition
persistent identity: BBa K566020
display id: BBa_K566020
version: 1
was generated by: iGEM to SBOL conversion
description: Mnt repressor regulated by pOmpC
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:12:43Z
mutable provenance: BioBricks.
owned by: james
owned by: myers
mutable description: Mnt repressor regulated by pOmpC
discontinued: false
dominant: false
group u list: _734_
m user id: 0
owner id: 8650
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: They were BioBricks.
star: false
creator: Daniel Rodriguez
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0015
component: BBa_C0072
component: BBa_B0034
component: BBa_R0082
sequence annotation: BBa_R0082
sequence annotation: BBa_B0034
sequence annotation: BBa_C0072
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K566020_sequence/1

BBa_B0015
type: Component
persistent identity: component2258816
display id: component2258816
version: 1
top level: BBa_K566020
definition: BBa_B0015
access: public

BBa_C0072
type: Component
persistent identity: component2258809
display id: component2258809
version: 1
top level: BBa_K566020
definition: mnt (s)
access: public

BBa_B0034
type: Component
persistent identity: component2258804
display id: component2258804
version: 1
top level: BBa_K566020
definition: BBa_B0034
access: public

BBa_R0082
type: Component
persistent identity: component2258802
display id: component2258802
version: 1
top level: BBa_K566020
definition: OmpR
access: public

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2258802
display id: annotation2258802
version: 1
top level: BBa_K566020
location: https://synbiohub.org/public/igem/BBa_K566020/annotation2258802/range2258802/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_K566020/annotation2258802/range2258802/1
type: Range
persistent identity: range2258802
display id: range2258802
version: 1
top level: BBa_K566020
direction: direction/reverse
start: 1
end: 108
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2258804
display id: annotation2258804
version: 1
top level: BBa_K566020
location: https://synbiohub.org/public/igem/BBa_K566020/annotation2258804/range2258804/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K566020/annotation2258804/range2258804/1
type: Range
persistent identity: range2258804
display id: range2258804
version: 1
top level: BBa_K566020
direction: direction/reverse
start: 117
end: 128
orientation: inline

BBa_C0072
type: SequenceAnnotation
persistent identity: annotation2258809
display id: annotation2258809
version: 1
top level: BBa_K566020
location: https://synbiohub.org/public/igem/BBa_K566020/annotation2258809/range2258809/1
component: BBa_C0072

https://synbiohub.org/public/igem/BBa_K566020/annotation2258809/range2258809/1
type: Range
persistent identity: range2258809
display id: range2258809
version: 1
top level: BBa_K566020
direction: direction/reverse
start: 135
end: 447
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2258816
display id: annotation2258816
version: 1
top level: BBa_K566020
location: https://synbiohub.org/public/igem/BBa_K566020/annotation2258816/range2258816/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K566020/annotation2258816/range2258816/1
type: Range
persistent identity: range2258816
display id: range2258816
version: 1
top level: BBa_K566020
direction: direction/reverse
start: 456
end: 584
orientation: inline

BBa_K566023
type: ComponentDefinition
persistent identity: BBa K566023
display id: BBa_K566023
version: 1
was generated by: iGEM to SBOL conversion
description: pcyA gene (constitutive expression)
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:12:43Z
mutable provenance: CDS was extracted by PCR, promoter and terminator were Classic BioBricks.
owned by: james
owned by: myers
mutable description: pcyA gene transcribed from constitutive promoter.
discontinued: false
dominant: false
experience: experience/None
group u list: _734_
m user id: 0
owner id: 8650
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: For the project that this part was made, it was needed to use other Assambly Standard.
star: false
creator: Daniel Rodriguez
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0015
component: BBa_K566021
component: BBa_K081005
sequence annotation: BBa_K081005
sequence annotation: BBa_K566021
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K566023_sequence/1

BBa_B0015
type: Component
persistent identity: component2142757
display id: component2142757
version: 1
top level: BBa_K566023
definition: BBa_B0015
access: public

BBa_K566021
type: Component
persistent identity: component2142750
display id: component2142750
version: 1
top level: BBa_K566023
definition: pcyA CDS
access: public

BBa_K081005
type: Component
persistent identity: component2142748
display id: component2142748
version: 1
top level: BBa_K566023
definition: BBa_K081005
access: public

BBa_K081005
type: SequenceAnnotation
persistent identity: annotation2142748
display id: annotation2142748
version: 1
top level: BBa_K566023
location: https://synbiohub.org/public/igem/BBa_K566023/annotation2142748/range2142748/1
component: BBa_K081005

https://synbiohub.org/public/igem/BBa_K566023/annotation2142748/range2142748/1
type: Range
persistent identity: range2142748
display id: range2142748
version: 1
top level: BBa_K566023
direction: direction/reverse
start: 1
end: 58
orientation: inline

BBa_K566021
type: SequenceAnnotation
persistent identity: annotation2142750
display id: annotation2142750
version: 1
top level: BBa_K566023
location: https://synbiohub.org/public/igem/BBa_K566023/annotation2142750/range2142750/1
component: BBa_K566021

https://synbiohub.org/public/igem/BBa_K566023/annotation2142750/range2142750/1
type: Range
persistent identity: range2142750
display id: range2142750
version: 1
top level: BBa_K566023
direction: direction/reverse
start: 65
end: 811
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2142757
display id: annotation2142757
version: 1
top level: BBa_K566023
location: https://synbiohub.org/public/igem/BBa_K566023/annotation2142757/range2142757/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K566023/annotation2142757/range2142757/1
type: Range
persistent identity: range2142757
display id: range2142757
version: 1
top level: BBa_K566023
direction: direction/reverse
start: 820
end: 948
orientation: inline

BBa_K566024
type: ComponentDefinition
persistent identity: BBa K566024
display id: BBa_K566024
version: 1
was generated by: iGEM to SBOL conversion
description: ho1 gene (constitutive expression)
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:12:43Z
mutable provenance: CDS was extracted by PCR, promoter and terminator were Classic BioBricks.
owned by: james
owned by: myers
mutable description: ho1 gene transcribed from constitutive promoter.
discontinued: false
dominant: false
experience: experience/None
group u list: _734_
m user id: 0
owner id: 8650
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: For the project that this part was made, it was needed to use other Assambly Standard.
star: false
creator: Daniel Rodriguez
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0015
component: BBa_K566022
component: BBa_K081005
sequence annotation: BBa_K081005
sequence annotation: BBa_K566022
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K566024_sequence/1

BBa_B0015
type: Component
persistent identity: component2142771
display id: component2142771
version: 1
top level: BBa_K566024
definition: BBa_B0015
access: public

BBa_K566022
type: Component
persistent identity: component2142764
display id: component2142764
version: 1
top level: BBa_K566024
definition: ho1 CDS
access: public

BBa_K081005
type: Component
persistent identity: component2142762
display id: component2142762
version: 1
top level: BBa_K566024
definition: BBa_K081005
access: public

BBa_K081005
type: SequenceAnnotation
persistent identity: annotation2142762
display id: annotation2142762
version: 1
top level: BBa_K566024
location: https://synbiohub.org/public/igem/BBa_K566024/annotation2142762/range2142762/1
component: BBa_K081005

https://synbiohub.org/public/igem/BBa_K566024/annotation2142762/range2142762/1
type: Range
persistent identity: range2142762
display id: range2142762
version: 1
top level: BBa_K566024
direction: direction/reverse
start: 1
end: 58
orientation: inline

BBa_K566022
type: SequenceAnnotation
persistent identity: annotation2142764
display id: annotation2142764
version: 1
top level: BBa_K566024
location: https://synbiohub.org/public/igem/BBa_K566024/annotation2142764/range2142764/1
component: BBa_K566022

https://synbiohub.org/public/igem/BBa_K566024/annotation2142764/range2142764/1
type: Range
persistent identity: range2142764
display id: range2142764
version: 1
top level: BBa_K566024
direction: direction/reverse
start: 65
end: 787
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2142771
display id: annotation2142771
version: 1
top level: BBa_K566024
location: https://synbiohub.org/public/igem/BBa_K566024/annotation2142771/range2142771/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K566024/annotation2142771/range2142771/1
type: Range
persistent identity: range2142771
display id: range2142771
version: 1
top level: BBa_K566024
direction: direction/reverse
start: 796
end: 924
orientation: inline

Cph8 gene
type: ComponentDefinition
persistent identity: BBa K566026
display id: BBa_K566026
version: 1
was generated by: iGEM to SBOL conversion
description: Cph8 gene for red-photoreceptor (constitutive expression)
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:12:43Z
mutable provenance: CDS of Cph8 was extracted by PCR, promoter and terminator were Classic BioBricks.
owned by: james
owned by: myers
mutable description: Cassette for constitutive expression of Cph8, originally constructed by Dr. Jeff Tabor. This gene codifies for the red light-sensing protein Cph8, which is expressed in the phosphorylated ground state. It is switched to the unphosphorylated state by 650-nm light and back to the phosphorylated state by 705-nm light. When phosphorylated, Cph8 passes a phosphoryl group to OmpR, which then binds to and activates transcription from PompC. Because it is inactivated by red light, Cph8 can be considered a logical (NOT red) sensor. A genetic inverter or logical NOT gate is used to invert the response of the (NOT red) sensor to that of a red light sensor
discontinued: false
dominant: false
experience: experience/None
group u list: _734_
m user id: 0
owner id: 8650
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: For the project that this part was made, it was needed to use other Assambly Standard.
star: false
creator: Daniel Rodriguez
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0015
component: BBa_K566025
component: BBa_K081005
sequence annotation: BBa_K081005
sequence annotation: BBa_K566025
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K566026_sequence/1

BBa_B0015
type: Component
persistent identity: component2142837
display id: component2142837
version: 1
top level: Cph8 gene
definition: BBa_B0015
access: public

BBa_K566025
type: Component
persistent identity: component2142830
display id: component2142830
version: 1
top level: Cph8 gene
definition: Cph8
access: public

BBa_K081005
type: Component
persistent identity: component2142828
display id: component2142828
version: 1
top level: Cph8 gene
definition: BBa_K081005
access: public

BBa_K081005
type: SequenceAnnotation
persistent identity: annotation2142828
display id: annotation2142828
version: 1
top level: Cph8 gene
location: https://synbiohub.org/public/igem/BBa_K566026/annotation2142828/range2142828/1
component: BBa_K081005

https://synbiohub.org/public/igem/BBa_K566026/annotation2142828/range2142828/1
type: Range
persistent identity: range2142828
display id: range2142828
version: 1
top level: Cph8 gene
direction: direction/reverse
start: 1
end: 58
orientation: inline

BBa_K566025
type: SequenceAnnotation
persistent identity: annotation2142830
display id: annotation2142830
version: 1
top level: Cph8 gene
location: https://synbiohub.org/public/igem/BBa_K566026/annotation2142830/range2142830/1
component: BBa_K566025

https://synbiohub.org/public/igem/BBa_K566026/annotation2142830/range2142830/1
type: Range
persistent identity: range2142830
display id: range2142830
version: 1
top level: Cph8 gene
direction: direction/reverse
start: 65
end: 2299
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2142837
display id: annotation2142837
version: 1
top level: Cph8 gene
location: https://synbiohub.org/public/igem/BBa_K566026/annotation2142837/range2142837/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K566026/annotation2142837/range2142837/1
type: Range
persistent identity: range2142837
display id: range2142837
version: 1
top level: Cph8 gene
direction: direction/reverse
start: 2308
end: 2436
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

OmpR
type: ComponentDefinition
persistent identity: BBa R0082
display id: BBa_R0082
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (OmpR, positive)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: NC_000193 E. coli K12
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1. An alternate version, BBa_R0083, cuts out the C2 and C3 sites. The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown.
star: true
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: C1 OmpR
sequence annotation: C2 OmpR
sequence annotation: C3 OmpR
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0082_sequence/1

C1 OmpR
type: SequenceAnnotation
persistent identity: annotation301154
display id: annotation301154
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
type: Range
persistent identity: range301154
display id: range301154
version: 1
top level: OmpR
direction: direction/forward
start: 13
end: 30
orientation: inline

C2 OmpR
type: SequenceAnnotation
persistent identity: annotation301155
display id: annotation301155
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
type: Range
persistent identity: range301155
display id: range301155
version: 1
top level: OmpR
direction: direction/forward
start: 34
end: 51
orientation: inline

C3 OmpR
type: SequenceAnnotation
persistent identity: annotation301156
display id: annotation301156
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
type: Range
persistent identity: range301156
display id: range301156
version: 1
top level: OmpR
direction: direction/forward
start: 54
end: 71
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301166
display id: annotation301166
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
type: Range
persistent identity: range301166
display id: range301166
version: 1
top level: OmpR
direction: direction/forward
start: 75
end: 80
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301167
display id: annotation301167
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
type: Range
persistent identity: range301167
display id: range301167
version: 1
top level: OmpR
direction: direction/forward
start: 98
end: 103
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

mnt (s)
type: ComponentDefinition
persistent identity: BBa C0072
display id: BBa_C0072
version: 1
was generated by: iGEM to SBOL conversion
description: mnt repressor (strong) from Salmonella phage P22 (+LVA)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: enterobacteriophage p22
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: wild type dimeric repressor from enterobacteriophage p22 with dissociation constant of 2.5*10^-12. sequence was translated with the E.Coli K12 codon usage table, and nucleotides 95-97 was changed from AAT to AAC to avoid the cut site GAATTC (94-99). Identification of Functionally Important Residues in the DNA Binding Region of the MNT Repressor Kendall, KL; Sauer, RT The Journal of Biological Chemistry, 264(23):13706-13710, 1989
star: true
creator: crackdots
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mnt
sequence annotation: T
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0072_sequence/1

mnt
type: SequenceAnnotation
persistent identity: annotation308499
display id: annotation308499
version: 1
top level: mnt (s)
location: https://synbiohub.org/public/igem/BBa_C0072/annotation308499/range308499/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0072/annotation308499/range308499/1
type: Range
persistent identity: range308499
display id: range308499
version: 1
top level: mnt (s)
direction: direction/forward
start: 1
end: 249
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation308502
display id: annotation308502
version: 1
top level: mnt (s)
location: https://synbiohub.org/public/igem/BBa_C0072/annotation308502/range308502/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0072/annotation308502/range308502/1
type: Range
persistent identity: range308502
display id: range308502
version: 1
top level: mnt (s)
direction: direction/forward
start: 95
end: 97
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation308503
display id: annotation308503
version: 1
top level: mnt (s)
location: https://synbiohub.org/public/igem/BBa_C0072/annotation308503/range308503/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0072/annotation308503/range308503/1
type: Range
persistent identity: range308503
display id: range308503
version: 1
top level: mnt (s)
direction: direction/forward
start: 250
end: 282
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214010
display id: annotation2214010
version: 1
top level: mnt (s)
location: https://synbiohub.org/public/igem/BBa_C0072/annotation2214010/range2214010/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0072/annotation2214010/range2214010/1
type: Range
persistent identity: range2214010
display id: range2214010
version: 1
top level: mnt (s)
direction: direction/forward
start: 289
end: 313
orientation: inline

BBa_K081005
type: ComponentDefinition
persistent identity: BBa K081005
display id: BBa_K081005
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member and RBS
created: 2008-10-17T11:00:00Z
modified: 2015-05-08T01:08:34Z
mutable provenance: Promoter: John Anderson. RBS: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
owned by: myers
owned by: james
part status: Released HQ 2013
mutable description: Constitutive promoter (strong) with RBS (strong, efficiency=0.3)
discontinued: false
dominant: true
experience: experience/None
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: We used BioBrick Standard Assembly.
star: true
creator: Lorenzo Pasotti, Paolo Magni
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0030
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_K081005_sequence/1

BBa_B0030
type: Component
persistent identity: component1981865
display id: component1981865
version: 1
top level: BBa_K081005
definition: BBa_B0030
access: public

BBa_J23100
type: Component
persistent identity: component1981863
display id: component1981863
version: 1
top level: BBa_K081005
definition: BBa_J23100
access: public

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation1981863
display id: annotation1981863
version: 1
top level: BBa_K081005
location: https://synbiohub.org/public/igem/BBa_K081005/annotation1981863/range1981863/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K081005/annotation1981863/range1981863/1
type: Range
persistent identity: range1981863
display id: range1981863
version: 1
top level: BBa_K081005
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1981865
display id: annotation1981865
version: 1
top level: BBa_K081005
location: https://synbiohub.org/public/igem/BBa_K081005/annotation1981865/range1981865/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081005/annotation1981865/range1981865/1
type: Range
persistent identity: range1981865
display id: range1981865
version: 1
top level: BBa_K081005
direction: direction/forward
start: 44
end: 58
orientation: inline

pcyA CDS
type: ComponentDefinition
persistent identity: BBa K566021
display id: BBa_K566021
version: 1
was generated by: iGEM to SBOL conversion
description: pcyA CDS
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:12:43Z
mutable provenance: pPLPCB(S) plasmid from Dr. Jeff Tabor
owned by: james
owned by: myers
mutable description: pcyA CDS
discontinued: false
dominant: false
experience: experience/None
group u list: _734_
m user id: 0
owner id: 8650
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: No considerations.
star: false
creator: Daniel Rodriguez
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: pcyA
sequence: https://synbiohub.org/public/igem/BBa_K566021_sequence/1

pcyA
type: SequenceAnnotation
persistent identity: annotation2142741
display id: annotation2142741
version: 1
top level: pcyA CDS
location: https://synbiohub.org/public/igem/BBa_K566021/annotation2142741/range2142741/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K566021/annotation2142741/range2142741/1
type: Range
persistent identity: range2142741
display id: range2142741
version: 1
top level: pcyA CDS
direction: direction/forward
start: 1
end: 747
orientation: inline

ho1 CDS
type: ComponentDefinition
persistent identity: BBa K566022
display id: BBa_K566022
version: 1
was generated by: iGEM to SBOL conversion
description: ho1 CDS
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:12:43Z
mutable provenance: Extracted by PCR from pPLPCB(S) plasmid of Dr. Jeff Tabor.
owned by: james
owned by: myers
mutable description: ho1 CDS
discontinued: false
dominant: false
experience: experience/None
group u list: _734_
m user id: 0
owner id: 8650
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: No considerations were needed.
star: false
creator: Daniel Rodriguez
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: ho1
sequence: https://synbiohub.org/public/igem/BBa_K566022_sequence/1

ho1
type: SequenceAnnotation
persistent identity: annotation2142743
display id: annotation2142743
version: 1
top level: ho1 CDS
location: https://synbiohub.org/public/igem/BBa_K566022/annotation2142743/range2142743/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K566022/annotation2142743/range2142743/1
type: Range
persistent identity: range2142743
display id: range2142743
version: 1
top level: ho1 CDS
direction: direction/forward
start: 1
end: 723
orientation: inline

Cph8
type: ComponentDefinition
persistent identity: BBa K566025
display id: BBa_K566025
version: 1
was generated by: iGEM to SBOL conversion
description: Cph8 CDS
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:12:43Z
mutable provenance: Extracted by PCR directly from pCph8 plasmid of Dr. Jeff Tabor.
owned by: james
owned by: myers
mutable description: The red light-sensing protein Cph8 is expressed in the phosphorylated ground state. It is switched to the unphosphorylated state by 650-nm light and back to the phosphorylated state by 705-nm light. When phosphorylated, Cph8 passes a phosphoryl group to OmpR, which then binds to and activates transcription from PompC. Because it is inactivated by red light, Cph8 can be considered a logical (NOT red) sensor. A genetic inverter or logical NOT gate is used to invert the response of the (NOT red) sensor to that of a red light sensor
discontinued: false
dominant: false
experience: experience/None
group u list: _734_
m user id: 0
owner id: 8650
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: For the project that this part was made, it was needed to use other Assambly Standard.
star: false
creator: Daniel Rodriguez
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Cph8
sequence: https://synbiohub.org/public/igem/BBa_K566025_sequence/1

Cph8
type: SequenceAnnotation
persistent identity: annotation2142823
display id: annotation2142823
version: 1
top level: Cph8
location: https://synbiohub.org/public/igem/BBa_K566025/annotation2142823/range2142823/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K566025/annotation2142823/range2142823/1
type: Range
persistent identity: range2142823
display id: range2142823
version: 1
top level: Cph8
direction: direction/forward
start: 1
end: 2235
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

https://synbiohub.org/public/igem/BBa_K566019_sequence/1
type: Sequence
persistent identity: BBa K566019 sequence
display id: BBa_K566019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccgcccacctgattcagccccacggtttagtggtggtcctgcaggaaccagacctcaccatcagccaaattagcgccaactgcaccggcattttagggcgatcgccagaggatttgttgggcagaaccctaggggaagtgtttgatagctttcagattgatcccatccagagtcgcctaacggccggacaaatcagcagcctcaaccccagtaaactttgggcgcgggtcatgggggacgactttgtcatttttgacggggtttttcatcgcaacagtgacggtttattggtatgtgaactagagccagcctacacttccgataatctgcccttcctcggtttttatcacatggccaacgctgccctgaatcggttgcgccaacaagctaatctacgggatttctacgatgttattgtcgaagaagtccgccgtatgactggctttgaccgggtgatgctataccgctttgatgaaaataaccacggtgatgtcattgccgaagataaacgggatgatatggaaccctatttgggcctgcactatcccgaatcggatattccccaacccgcccgtcggctatttatccacaaccccattcgagtaattcccgatgtttatggtgtggcggtgcccctgaccccagcggttaaccccagcaccaaccgagcggtggatttaacagaatccattctgcgcagtgcgtaccattgccacttgacctatctgaaaaatatgggggtaggagcgtctttaaccatttccctaattaaggacggccatctctgggggctcattgcctgccaccatcaaacccccaaagtaattccctttgaactgcgtaaagcctgcgaattttttggtcgggtggtgtttagcaacatttccgcccaggaagatacggaaaccttcgattaccgggtgcagttggcggagcatgaagcggttttattggacaaaatgaccacggcggcggattttgtcgaaggattaactaatcatcccgatcgcctgttgggattaacgggctcccagggggcggccatttgctttggggaaaaattgattttagtaggggaaaccccggacgagaaagcagtgcaatatttactgcaatggttggagaatcgggaagtgcaagacgttttcttcacctcttccctctcacaaatttatcctgatgcagtgaattttaaatccgtggccagtggcttattggccattcccattgcccgtcacaactttttgctctggtttcgccctgaagtgttgcaaacggttaattggggcggtgacccaaatcatgcttacgaagctacccaggaagacggtaaaatcgagctccatccccgccaatcctttgacctctggaaagaaattgtccgactccaatctttgccctggcaatcggtggaaatccaaagtgccctggccctgaaaaaggcgatcgtcaacctcattttgcgccaggcagaagaattgcatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K566020_sequence/1
type: Sequence
persistent identity: BBa K566020 sequence
display id: BBa_K566020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggacttactagagaaagaggagaaatactagatggcccgggatgatcctcacttcaattttcgtatgccaatggaagtaagagagaaattgaaatttagagcagaggcaaacggacggagcatgaactctgagcttttgcaaatcgtacaagatgccctaagcaaaccgtcaccagtcactgggtaccgcaatgatgcggaacgactcgccgatgagcagagcgagttagtgaagaagatggtcttcgatacactgaaggatctttataaaaaaaccaccgctgcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K566023_sequence/1
type: Sequence
persistent identity: BBa K566023 sequence
display id: BBa_K566023_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K566024_sequence/1
type: Sequence
persistent identity: BBa K566024 sequence
display id: BBa_K566024_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K566026_sequence/1
type: Sequence
persistent identity: BBa K566026 sequence
display id: BBa_K566026_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccgcccacctgattcagccccacggtttagtggtggtcctgcaggaaccagacctcaccatcagccaaattagcgccaactgcaccggcattttagggcgatcgccagaggatttgttgggcagaaccctaggggaagtgtttgatagctttcagattgatcccatccagagtcgcctaacggccggacaaatcagcagcctcaaccccagtaaactttgggcgcgggtcatgggggacgactttgtcatttttgacggggtttttcatcgcaacagtgacggtttattggtatgtgaactagagccagcctacacttccgataatctgcccttcctcggtttttatcacatggccaacgctgccctgaatcggttgcgccaacaagctaatctacgggatttctacgatgttattgtcgaagaagtccgccgtatgactggctttgaccgggtgatgctataccgctttgatgaaaataaccacggtgatgtcattgccgaagataaacgggatgatatggaaccctatttgggcctgcactatcccgaatcggatattccccaacccgcccgtcggctatttatccacaaccccattcgagtaattcccgatgtttatggtgtggcggtgcccctgaccccagcggttaaccccagcaccaaccgagcggtggatttaacagaatccattctgcgcagtgcgtaccattgccacttgacctatctgaaaaatatgggggtaggagcgtctttaaccatttccctaattaaggacggccatctctgggggctcattgcctgccaccatcaaacccccaaagtaattccctttgaactgcgtaaagcctgcgaattttttggtcgggtggtgtttagcaacatttccgcccaggaagatacggaaaccttcgattaccgggtgcagttggcggagcatgaagcggttttattggacaaaatgaccacggcggcggattttgtcgaaggattaactaatcatcccgatcgcctgttgggattaacgggctcccagggggcggccatttgctttggggaaaaattgattttagtaggggaaaccccggacgagaaagcagtgcaatatttactgcaatggttggagaatcgggaagtgcaagacgttttcttcacctcttccctctcacaaatttatcctgatgcagtgaattttaaatccgtggccagtggcttattggccattcccattgcccgtcacaactttttgctctggtttcgccctgaagtgttgcaaacggttaattggggcggtgacccaaatcatgcttacgaagctacccaggaagacggtaaaatcgagctccatccccgccaatcctttgacctctggaaagaaattgtccgactccaatctttgccctggcaatcggtggaaatccaaagtgccctggccctgaaaaaggcgatcgtcaacctcattttgcgccaggcagaagaattgcatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0082_sequence/1
type: Sequence
persistent identity: BBa R0082 sequence
display id: BBa_R0082_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0072_sequence/1
type: Sequence
persistent identity: BBa C0072 sequence
display id: BBa_C0072_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcccgggatgatcctcacttcaattttcgtatgccaatggaagtaagagagaaattgaaatttagagcagaggcaaacggacggagcatgaactctgagcttttgcaaatcgtacaagatgccctaagcaaaccgtcaccagtcactgggtaccgcaatgatgcggaacgactcgccgatgagcagagcgagttagtgaagaagatggtcttcgatacactgaaggatctttataaaaaaaccaccgctgcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K081005_sequence/1
type: Sequence
persistent identity: BBa K081005 sequence
display id: BBa_K081005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K566021_sequence/1
type: Sequence
persistent identity: BBa K566021 sequence
display id: BBa_K566021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K566022_sequence/1
type: Sequence
persistent identity: BBa K566022 sequence
display id: BBa_K566022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K566025_sequence/1
type: Sequence
persistent identity: BBa K566025 sequence
display id: BBa_K566025_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccgcccacctgattcagccccacggtttagtggtggtcctgcaggaaccagacctcaccatcagccaaattagcgccaactgcaccggcattttagggcgatcgccagaggatttgttgggcagaaccctaggggaagtgtttgatagctttcagattgatcccatccagagtcgcctaacggccggacaaatcagcagcctcaaccccagtaaactttgggcgcgggtcatgggggacgactttgtcatttttgacggggtttttcatcgcaacagtgacggtttattggtatgtgaactagagccagcctacacttccgataatctgcccttcctcggtttttatcacatggccaacgctgccctgaatcggttgcgccaacaagctaatctacgggatttctacgatgttattgtcgaagaagtccgccgtatgactggctttgaccgggtgatgctataccgctttgatgaaaataaccacggtgatgtcattgccgaagataaacgggatgatatggaaccctatttgggcctgcactatcccgaatcggatattccccaacccgcccgtcggctatttatccacaaccccattcgagtaattcccgatgtttatggtgtggcggtgcccctgaccccagcggttaaccccagcaccaaccgagcggtggatttaacagaatccattctgcgcagtgcgtaccattgccacttgacctatctgaaaaatatgggggtaggagcgtctttaaccatttccctaattaaggacggccatctctgggggctcattgcctgccaccatcaaacccccaaagtaattccctttgaactgcgtaaagcctgcgaattttttggtcgggtggtgtttagcaacatttccgcccaggaagatacggaaaccttcgattaccgggtgcagttggcggagcatgaagcggttttattggacaaaatgaccacggcggcggattttgtcgaaggattaactaatcatcccgatcgcctgttgggattaacgggctcccagggggcggccatttgctttggggaaaaattgattttagtaggggaaaccccggacgagaaagcagtgcaatatttactgcaatggttggagaatcgggaagtgcaagacgttttcttcacctcttccctctcacaaatttatcctgatgcagtgaattttaaatccgtggccagtggcttattggccattcccattgcccgtcacaactttttgctctggtttcgccctgaagtgttgcaaacggttaattggggcggtgacccaaatcatgcttacgaagctacccaggaagacggtaaaatcgagctccatccccgccaatcctttgacctctggaaagaaattgtccgactccaatctttgccctggcaatcggtggaaatccaaagtgccctggccctgaaaaaggcgatcgtcaacctcattttgcgccaggcagaagaattgcatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctggatcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaaga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z