##gff-version 3
##sequence-region BBa_K566020 1 584
BBa_K566020	.	promoter	1	108	.	+	0	ID=BBa_R0082;Name=BBa_R0082
BBa_K566020	.	promoter	98	103	.	+	0	ID=annotation301167;Name=-10;Parent=BBa_R0082
BBa_K566020	.	promoter	75	80	.	+	0	ID=annotation301166;Name=-35;Parent=BBa_R0082
BBa_K566020	.	non_covalent_binding_site	13	30	.	+	0	ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082
BBa_K566020	.	non_covalent_binding_site	34	51	.	+	0	ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082
BBa_K566020	.	non_covalent_binding_site	54	71	.	+	0	ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082
BBa_K566020	.	ribosome_entry_site	117	128	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K566020	.	conserved_region	121	124	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K566020	.	terminator	456	584	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K566020	.	terminator	544	584	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K566020	.	engineered_region	544	584	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K566020	.	stop_codon	577	577	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K566020	.	stem_loop	551	570	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K566020	.	polyA_site	571	584	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K566020	.	terminator	456	535	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K566020	.	engineered_region	456	535	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K566020	.	stem_loop	467	510	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K566020	.	CDS	135	447	.	+	0	ID=BBa_C0072;Name=BBa_C0072
BBa_K566020	.	CDS	135	383	.	+	0	ID=annotation308499;Name=mnt;Parent=BBa_C0072
BBa_K566020	.	point_mutation	229	231	.	+	0	ID=annotation308502;Name=T;Parent=BBa_C0072
BBa_K566020	.	tag	384	416	.	+	0	ID=annotation308503;Name=LVA;Parent=BBa_C0072
BBa_K566020	.	engineered_tag	423	447	.	+	0	ID=annotation2214010;Name=Help:Barcodes;Parent=BBa_C0072
>BBa_K566020
tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttgga
ttattctgcatttttggggagaatggacttactagagaaagaggagaaatactagatggcccgggatgatcctcacttc
aattttcgtatgccaatggaagtaagagagaaattgaaatttagagcagaggcaaacggacggagcatgaactctgagc
ttttgcaaatcgtacaagatgccctaagcaaaccgtcaccagtcactgggtaccgcaatgatgcggaacgactcgccga
tgagcagagcgagttagtgaagaagatggtcttcgatacactgaaggatctttataaaaaaaccaccgctgcaaacgac
gaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccctactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttata