LOCUS       BBa_K566023              948 bp    DNA     linear       29-Apr-2024
DEFINITION  pcyA gene (constitutive expression)
ACCESSION   BBa_K566023
VERSION     BBa_K566023.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     RBS             44..58
                     /label=RBS-1\Strong
     RBS             51..55
                     /label=RBS
     misc_feature    44..58
                     /label=BBa_B0030
     RBS             44..58
                     /label=BBa_B0030
     misc_feature    1..58
                     /label=BBa_K081005
     CDS             65..811
                     /label=pcyA
     CDS             65..811
                     /label=BBa_K566021
     stem_loop       831..874
                     /label=stem_loop
     misc_feature    820..899
                     /label=BBa_B0010
     terminator      820..899
                     /label=BBa_B0010
     stem_loop       915..934
                     /label=T7 TE
     misc_feature    941..941
                     /label=stop
     polyA_site      935..948
                     /label=polya
     misc_feature    908..948
                     /label=BBa_B0012
     terminator      908..948
                     /label=BBa_B0012
     terminator      820..948
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagattaaag aggagaaata
       61 ctagatggcc gtcactgatt taagtttgac caattcttcc ctgatgccta cgttgaaccc
      121 gatgattcaa cagttggccc tggcgatcgc cgctagttgg caaagtttac ccctcaagcc
      181 ctatcaattg ccggaggatt tgggctacgt agaaggccgc ctggaagggg aaaagttagt
      241 gattgaaaat cggtgctacc aaacgcccca gtttcgcaaa atgcatttgg agttggccaa
      301 ggtgggcaaa gggttggata ttctccactg tgtaatgttt cctgagcctt tatacggtct
      361 acctttgttt ggctgtgaca ttgtggccgg ccccggtgga gtaagtgcgg ctattgcgga
      421 tctatccccc acccaaagcg atcgccaatt gcccgcagcg taccaaaaat cattggcaga
      481 gctaggccag ccagaatttg agcaacaacg ggaattgccc ccctggggag aaatattttc
      541 tgaatattgt ttattcatcc gtcccagcaa tgtcactgaa gaagaaagat ttgtacaaag
      601 ggtagtggac tttttgcaaa ttcattgtca ccaatccatc gttgccgaac ccttgtctga
      661 agctcaaact ttggagcacc gtcaggggca aattcattac tgccaacaac aacagaaaaa
      721 tgataaaacc cgtcgggtac tggaaaaagc ttttggggaa gcttgggcgg aacggtatat
      781 gagccaagtc ttatttgatg ttatccaata atactagagc caggcatcaa ataaaacgaa
      841 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
      901 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//