LOCUS       BBa_K566030             2463 bp    DNA     linear       25-Nov-2024
DEFINITION  CcaS gene (constitutive expression)
ACCESSION   BBa_K566030
VERSION     BBa_K566030.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     RBS             44..58
                     /label=RBS-1\Strong
     RBS             51..55
                     /label=RBS
     misc_feature    44..58
                     /label=BBa_B0030
     RBS             44..58
                     /label=BBa_B0030
     misc_feature    1..58
                     /label=BBa_K081005
     misc_feature    65..67
                     /label=Start
     misc_feature    2324..2326
                     /label=Stop
     CDS             65..2326
                     /label=cds
     CDS             65..2326
                     /label=BBa_K566027
     stem_loop       2346..2389
                     /label=stem_loop
     misc_feature    2335..2414
                     /label=BBa_B0010
     terminator      2335..2414
                     /label=BBa_B0010
     stem_loop       2430..2449
                     /label=T7 TE
     misc_feature    2456..2456
                     /label=stop
     polyA_site      2450..2463
                     /label=polya
     misc_feature    2423..2463
                     /label=BBa_B0012
     terminator      2423..2463
                     /label=BBa_B0012
     terminator      2335..2463
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagattaaag aggagaaata
       61 ctagatgggc aaatttctaa ttccaatcga atttgttttt ctggcgatcg ccatgacctg
      121 ttatttatgg cacagacaaa accaagaacg ccgcaggatt gaaattagca tcaagcaaca
      181 aacccaacgg gaacgattta ttaaccaaat tacccaacat atccgccaat ctttaaactt
      241 ggaaacggtt ttaaatacca ccgtcgctga agttaaaacc ctgttgcaag ttgatcgagt
      301 tctaatttat cgcatttggc aagatggcac gggcagcgcc attacggaat cggtgaatgc
      361 caattatcct agtattttag ggcggacctt ttccgatgaa gtttttcccg ttgaatacca
      421 tcaagcctac accaaaggta aagtacgggc cattaatgac attgaccagg atgacataga
      481 gatttgccta gctgatttcg tcaaacaatt tggcgtgaaa tcaaaattag tagtgcccat
      541 tcttcaacat aatcgtgctt cttccctaga taatgaatca gaatttccct atctttgggg
      601 gctgttaatt acccatcaat gtgcttttac ccggccatgg caaccgtggg aagtggagtt
      661 aatgaaacag ctagccaatc aggtcgcgat cgccatccaa caatcggaat tatatgagca
      721 attacagcaa ctcaataaag atttggaaaa ccgagtcgaa aaacgcaccc agcaacttgc
      781 cgccaccaat caatccctaa gaatggaaat cagtgagcga caaaaaacgg aagccgctct
      841 ccgccacact aaccatactc tgcaatccct gattgcggcc tcccccaggg gtatttttac
      901 ccttaattta gcagaccaaa ttcagatttg gaatcctaca gcagaacgta tttttggttg
      961 gacagaaaca gaaattattg cccatccaga attattaaca tccaacattt tgctggaaga
     1021 ttatcagcaa tttaaacaga aagttttatc aggcatggtt tcccctagcc tagaattaaa
     1081 atgtcaaaaa aaagatggta gttggattga aattgtcctt tccgctgctc ccctattgga
     1141 tagtgaagaa aatattgccg gattggtggc ggttgtcgcc gatattaccg agcaaaagcg
     1201 gcaggcagaa caaattcgtt tgctacaatc cgttgtggtt aatactaatg atgcggtggt
     1261 gattacggaa gcggagccca ttgatgatcc cgggccgaga attctctatg tcaatgaagc
     1321 atttactaaa atcaccggtt atactgctga agaaatgcta ggcaaaaccc cccgagtttt
     1381 acagggacca aaaactagtc gcactgaatt agatagggtg cggcaagcca ttagtcaatg
     1441 gcaatcagtt accgttgaag tgattaatta tcgtaaggat ggcagtgagt tttgggtgga
     1501 atttagtctg gtgcccgttg ccaataaaac aggtttttac acccattgga ttgctgtgca
     1561 aagggatgtc actgagcgcc gacgcacgga ggaagtccgc ctagctttag aacgggaaaa
     1621 agaattaagc cgcctaaaaa ctcgtttttt ctccatggct tcccatgaat ttcgtactcc
     1681 cctcagtacg gccttagctg ctgcccaatt actggaaaat tctgaagtgg cctggcttga
     1741 tcccgataag cgtagccgga acttacaccg tattcaaaat tccgtgaaaa atatggtaca
     1801 gctcctggat gatattttaa tcattaaccg tgccgaagcg ggcaaattgg aatttaatcc
     1861 taattggtta gatttgaaat tattgttcca gcaatttatc gaagaaattc aattaagtgt
     1921 cagtgaccaa tattattttg actttatttg tagcgctcaa gatacgaagg cattggtgga
     1981 tgaaaggtta gtgcggtcta ttttatctaa tctgttatct aatgcgatta aatactctcc
     2041 cgggggaggg cagattaaaa ttgccctaag cctagattcg gaacagatta tttttgaagt
     2101 caccgaccag ggcattggca tttcgccaga ggaccaaaag caaatttttg aaccctttca
     2161 tcggggcaaa aatgtcagaa atattacggg aacaggactc ggtttaatgg ttgccaagaa
     2221 atgtgttgac ttacacagtg gcagtatctt gctaaaaagt gcagttgacc agggaacaac
     2281 agttactatc tgtttaaaac gctataacca tttgcctcga gcttagtact agagccaggc
     2341 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     2401 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     2461 ata
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