##gff-version 3
##sequence-region BBa_K566031 1 5265
BBa_K566031	.	engineered_region	2803	5265	.	+	0	ID=BBa_K566030;Name=BBa_K566030
BBa_K566031	.	CDS	2867	5128	.	+	0	ID=BBa_K566027;Name=BBa_K566027;Parent=BBa_K566030
BBa_K566031	.	start_codon	2867	2869	.	+	0	ID=annotation2145564;Name=Start;Parent=BBa_K566027
BBa_K566031	.	CDS	2867	5128	.	+	0	ID=annotation2145566;Name=cds;Parent=BBa_K566027
BBa_K566031	.	stop_codon	5126	5128	.	+	0	ID=annotation2145565;Name=Stop;Parent=BBa_K566027
BBa_K566031	.	engineered_region	2803	2860	.	+	0	ID=BBa_K081005;Name=BBa_K081005;Parent=BBa_K566030
BBa_K566031	.	promoter	2803	2837	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K081005
BBa_K566031	.	ribosome_entry_site	2846	2860	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081005
BBa_K566031	.	engineered_region	2846	2860	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K566031	.	ribosome_entry_site	2853	2857	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K566031	.	ribosome_entry_site	2846	2860	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K566031	.	terminator	5137	5265	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K566030
BBa_K566031	.	terminator	5225	5265	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K566031	.	polyA_site	5252	5265	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K566031	.	stem_loop	5232	5251	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K566031	.	stop_codon	5258	5258	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K566031	.	engineered_region	5225	5265	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K566031	.	terminator	5137	5216	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K566031	.	stem_loop	5148	5191	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K566031	.	engineered_region	5137	5216	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K566031	.	engineered_region	1889	2794	.	+	0	ID=BBa_K566029;Name=BBa_K566029
BBa_K566031	.	CDS	1953	2657	.	+	0	ID=BBa_K566028;Name=BBa_K566028;Parent=BBa_K566029
BBa_K566031	.	CDS	1953	2657	.	+	0	ID=annotation2145570;Name=CDS;Parent=BBa_K566028
BBa_K566031	.	stop_codon	2655	2657	.	+	0	ID=annotation2145569;Name=Stop;Parent=BBa_K566028
BBa_K566031	.	start_codon	1953	1955	.	+	0	ID=annotation2145571;Name=Start;Parent=BBa_K566028
BBa_K566031	.	terminator	2666	2794	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K566029
BBa_K566031	.	terminator	2754	2794	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K566031	.	polyA_site	2781	2794	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K566031	.	stem_loop	2761	2780	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K566031	.	stop_codon	2787	2787	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K566031	.	engineered_region	2754	2794	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K566031	.	terminator	2666	2745	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K566031	.	stem_loop	2677	2720	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K566031	.	engineered_region	2666	2745	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K566031	.	engineered_region	1889	1946	.	+	0	ID=BBa_K081005;Name=BBa_K081005;Parent=BBa_K566029
BBa_K566031	.	promoter	1889	1923	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K081005
BBa_K566031	.	ribosome_entry_site	1932	1946	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081005
BBa_K566031	.	engineered_region	1932	1946	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K566031	.	ribosome_entry_site	1939	1943	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K566031	.	ribosome_entry_site	1932	1946	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K566031	.	engineered_region	957	1880	.	+	0	ID=BBa_K566024;Name=BBa_K566024
BBa_K566031	.	terminator	1752	1880	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K566024
BBa_K566031	.	terminator	1840	1880	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K566031	.	polyA_site	1867	1880	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K566031	.	stem_loop	1847	1866	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K566031	.	stop_codon	1873	1873	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K566031	.	engineered_region	1840	1880	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K566031	.	terminator	1752	1831	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K566031	.	stem_loop	1763	1806	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K566031	.	engineered_region	1752	1831	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K566031	.	engineered_region	957	1014	.	+	0	ID=BBa_K081005;Name=BBa_K081005;Parent=BBa_K566024
BBa_K566031	.	promoter	957	991	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K081005
BBa_K566031	.	ribosome_entry_site	1000	1014	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081005
BBa_K566031	.	engineered_region	1000	1014	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K566031	.	ribosome_entry_site	1007	1011	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K566031	.	ribosome_entry_site	1000	1014	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K566031	.	CDS	1021	1743	.	+	0	ID=BBa_K566022;Name=BBa_K566022;Parent=BBa_K566024
BBa_K566031	.	CDS	1021	1743	.	+	0	ID=annotation2142743;Name=ho1;Parent=BBa_K566022
BBa_K566031	.	engineered_region	1	948	.	+	0	ID=BBa_K566023;Name=BBa_K566023
BBa_K566031	.	engineered_region	1	58	.	+	0	ID=BBa_K081005;Name=BBa_K081005;Parent=BBa_K566023
BBa_K566031	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K081005
BBa_K566031	.	ribosome_entry_site	44	58	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081005
BBa_K566031	.	engineered_region	44	58	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K566031	.	ribosome_entry_site	51	55	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K566031	.	ribosome_entry_site	44	58	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K566031	.	CDS	65	811	.	+	0	ID=BBa_K566021;Name=BBa_K566021;Parent=BBa_K566023
BBa_K566031	.	CDS	65	811	.	+	0	ID=annotation2142741;Name=pcyA;Parent=BBa_K566021
BBa_K566031	.	terminator	820	948	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K566023
BBa_K566031	.	terminator	908	948	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K566031	.	polyA_site	935	948	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K566031	.	stem_loop	915	934	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K566031	.	stop_codon	941	941	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K566031	.	engineered_region	908	948	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K566031	.	terminator	820	899	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K566031	.	stem_loop	831	874	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K566031	.	engineered_region	820	899	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K566031
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatggccgtcactgat
ttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagtt
ggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagtt
agtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttg
gatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccg
gtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcatt
ggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgttta
ttcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcacc
aatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaaca
acagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtc
ttatttgatgttatccaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgt
tttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
tactagagttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatgagtg
tcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgctt
cctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagag
gaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagc
aagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccg
agtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggc
ggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgaca
ttgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacg
gattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcg
atcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaag
gctagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgt
cggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggc
tagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatgagaattcttttagtggagga
tgatttgccgctggcggaaacccttgctgaagcattgagtgaccagctttacaccgttgatattgccaccgacgcttcc
ctcgcctgggactatgcctcccgactggaatatgacctcgttattttggatgtgatgctgccggagttggacgggatta
ccctctgtcaaaaatggcgatcgcacagttatttaatgccaattttgatgatgacagccagggatacgatcaatgataa
aatcacgggcttggatgcgggggcggatgattatgtggtcaagccagtggatttgggggagttatttgccagggtgcga
gctttgttgcgtcggggttgtgcaacgtgccaaccagttttagagtgggggccaatcaggttggatccaagcacctatg
aagttagttatgacaatgaggttttgtctttgacccgcaaggaatacagcattctggaattactactccgcaatggccg
tcgggtgctaagtcggagcatgattatcgatagtatctggaagttggagagtcccccagaggaagatacggttaaggtg
catgtgcggagtttgcgacaaaaattaaaaagtgccggtttatcagcagatgccattgaaacggtccatggcattgggt
atcgtctggccaatttaacggaaaaatctttgtgccaagggaaaaactagtactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtcctaggtacagtgctagctactaga
gattaaagaggagaaatactagatgggcaaatttctaattccaatcgaatttgtttttctggcgatcgccatgacctgt
tatttatggcacagacaaaaccaagaacgccgcaggattgaaattagcatcaagcaacaaacccaacgggaacgattta
ttaaccaaattacccaacatatccgccaatctttaaacttggaaacggttttaaataccaccgtcgctgaagttaaaac
cctgttgcaagttgatcgagttctaatttatcgcatttggcaagatggcacgggcagcgccattacggaatcggtgaat
gccaattatcctagtattttagggcggaccttttccgatgaagtttttcccgttgaataccatcaagcctacaccaaag
gtaaagtacgggccattaatgacattgaccaggatgacatagagatttgcctagctgatttcgtcaaacaatttggcgt
gaaatcaaaattagtagtgcccattcttcaacataatcgtgcttcttccctagataatgaatcagaatttccctatctt
tgggggctgttaattacccatcaatgtgcttttacccggccatggcaaccgtgggaagtggagttaatgaaacagctag
ccaatcaggtcgcgatcgccatccaacaatcggaattatatgagcaattacagcaactcaataaagatttggaaaaccg
agtcgaaaaacgcacccagcaacttgccgccaccaatcaatccctaagaatggaaatcagtgagcgacaaaaaacggaa
gccgctctccgccacactaaccatactctgcaatccctgattgcggcctcccccaggggtatttttacccttaatttag
cagaccaaattcagatttggaatcctacagcagaacgtatttttggttggacagaaacagaaattattgcccatccaga
attattaacatccaacattttgctggaagattatcagcaatttaaacagaaagttttatcaggcatggtttcccctagc
ctagaattaaaatgtcaaaaaaaagatggtagttggattgaaattgtcctttccgctgctcccctattggatagtgaag
aaaatattgccggattggtggcggttgtcgccgatattaccgagcaaaagcggcaggcagaacaaattcgtttgctaca
atccgttgtggttaatactaatgatgcggtggtgattacggaagcggagcccattgatgatcccgggccgagaattctc
tatgtcaatgaagcatttactaaaatcaccggttatactgctgaagaaatgctaggcaaaaccccccgagttttacagg
gaccaaaaactagtcgcactgaattagatagggtgcggcaagccattagtcaatggcaatcagttaccgttgaagtgat
taattatcgtaaggatggcagtgagttttgggtggaatttagtctggtgcccgttgccaataaaacaggtttttacacc
cattggattgctgtgcaaagggatgtcactgagcgccgacgcacggaggaagtccgcctagctttagaacgggaaaaag
aattaagccgcctaaaaactcgttttttctccatggcttcccatgaatttcgtactcccctcagtacggccttagctgc
tgcccaattactggaaaattctgaagtggcctggcttgatcccgataagcgtagccggaacttacaccgtattcaaaat
tccgtgaaaaatatggtacagctcctggatgatattttaatcattaaccgtgccgaagcgggcaaattggaatttaatc
ctaattggttagatttgaaattattgttccagcaatttatcgaagaaattcaattaagtgtcagtgaccaatattattt
tgactttatttgtagcgctcaagatacgaaggcattggtggatgaaaggttagtgcggtctattttatctaatctgtta
tctaatgcgattaaatactctcccgggggagggcagattaaaattgccctaagcctagattcggaacagattatttttg
aagtcaccgaccagggcattggcatttcgccagaggaccaaaagcaaatttttgaaccctttcatcggggcaaaaatgt
cagaaatattacgggaacaggactcggtttaatggttgccaagaaatgtgttgacttacacagtggcagtatcttgcta
aaaagtgcagttgaccagggaacaacagttactatctgtttaaaacgctataaccatttgcctcgagcttagtactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata