BBa_K566031
type: ComponentDefinition
persistent identity: BBa K566031
display id: BBa_K566031
version: 1
was generated by: iGEM to SBOL conversion
description: E. coli MXGreen
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:43Z
mutable provenance: JT2 strain, genes from BioBricks and Dr. Jeff Tabor plasmids
owned by: james
owned by: myers
mutable description: Derived from Dr. Jeff Tabor JT2 E. coli strain, this part is a built-in red-light induction system in E. coli made through chromosome insertion. Avoiding the need of any extra-chromosomal DNA when light-inducing gene expression offering several advantages to the researcher. It is proposed as a photo-chassis that could make useful tools in this field of light induction. Integration was performed for the genes pcyA, ho1, Cph8 and a Mnt working as follows: Genes ho1 and pcyA are responsible for the chromophore synthesis. Cph8 codes for the chimaeric red-light receptor. These three genes are constitutively expressed. Mnt repressor is expressed from pOmpC promoter, which stops being induced in presence of red-light. It is therefore used as a NOT-gate to regulate expression from pMnt.
discontinued: false
dominant: false
group u list: _734_
m user id: 0
owner id: 8650
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: UANLBricks for assambly of photochassis. Dr. Kuhlman integration protocol.
star: false
creator: Daniel Rodriguez
type: DnaRegion
role: SO:0000340
role: partType/Cell
component: BBa_K566030
component: BBa_K566029
component: BBa_K566024
component: BBa_K566023
sequence annotation: BBa_K566023
sequence annotation: BBa_K566024
sequence annotation: BBa_K566029
sequence annotation: BBa_K566030
sequence: https://synbiohub.org/public/igem/BBa_K566031_sequence/1

BBa_K566030
type: Component
persistent identity: component2145790
display id: component2145790
version: 1
top level: BBa_K566031
definition: BBa_K566030
access: public

BBa_K566029
type: Component
persistent identity: component2145773
display id: component2145773
version: 1
top level: BBa_K566031
definition: BBa_K566029
access: public

BBa_K566024
type: Component
persistent identity: component2145756
display id: component2145756
version: 1
top level: BBa_K566031
definition: BBa_K566024
access: public

BBa_K566023
type: Component
persistent identity: component2145741
display id: component2145741
version: 1
top level: BBa_K566031
definition: BBa_K566023
access: public

BBa_K566023
type: SequenceAnnotation
persistent identity: annotation2145741
display id: annotation2145741
version: 1
top level: BBa_K566031
location: https://synbiohub.org/public/igem/BBa_K566031/annotation2145741/range2145741/1
component: BBa_K566023

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persistent identity: range2145741
display id: range2145741
version: 1
top level: BBa_K566031
direction: direction/reverse
start: 1
end: 948
orientation: inline

BBa_K566024
type: SequenceAnnotation
persistent identity: annotation2145756
display id: annotation2145756
version: 1
top level: BBa_K566031
location: https://synbiohub.org/public/igem/BBa_K566031/annotation2145756/range2145756/1
component: BBa_K566024

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persistent identity: range2145756
display id: range2145756
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end: 1880
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BBa_K566029
type: SequenceAnnotation
persistent identity: annotation2145773
display id: annotation2145773
version: 1
top level: BBa_K566031
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component: BBa_K566029

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persistent identity: range2145773
display id: range2145773
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BBa_K566030
type: SequenceAnnotation
persistent identity: annotation2145790
display id: annotation2145790
version: 1
top level: BBa_K566031
location: https://synbiohub.org/public/igem/BBa_K566031/annotation2145790/range2145790/1
component: BBa_K566030

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persistent identity: range2145790
display id: range2145790
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top level: BBa_K566031
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end: 5265
orientation: inline

BBa_K566029
type: ComponentDefinition
persistent identity: BBa K566029
display id: BBa_K566029
version: 1
was generated by: iGEM to SBOL conversion
description: CcaR gene (constitutive expression)
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:43Z
mutable provenance: UANLBrick assambly
owned by: james
owned by: myers
mutable description: CcaR gene (constitutive expression)
discontinued: false
dominant: false
group u list: _734_
m user id: 0
owner id: 8650
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: UANLBrick assambly
star: false
creator: Daniel Rodriguez
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0015
component: BBa_K566028
component: BBa_K081005
sequence annotation: BBa_K081005
sequence annotation: BBa_K566028
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K566029_sequence/1

BBa_B0015
type: Component
persistent identity: component2145591
display id: component2145591
version: 1
top level: BBa_K566029
definition: BBa_B0015
access: public

BBa_K566028
type: Component
persistent identity: component2145584
display id: component2145584
version: 1
top level: BBa_K566029
definition: BBa_K566028
access: public

BBa_K081005
type: Component
persistent identity: component2145580
display id: component2145580
version: 1
top level: BBa_K566029
definition: BBa_K081005
access: public

BBa_K081005
type: SequenceAnnotation
persistent identity: annotation2145580
display id: annotation2145580
version: 1
top level: BBa_K566029
location: https://synbiohub.org/public/igem/BBa_K566029/annotation2145580/range2145580/1
component: BBa_K081005

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persistent identity: range2145580
display id: range2145580
version: 1
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direction: direction/reverse
start: 1
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orientation: inline

BBa_K566028
type: SequenceAnnotation
persistent identity: annotation2145584
display id: annotation2145584
version: 1
top level: BBa_K566029
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component: BBa_K566028

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persistent identity: range2145584
display id: range2145584
version: 1
top level: BBa_K566029
direction: direction/reverse
start: 65
end: 769
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2145591
display id: annotation2145591
version: 1
top level: BBa_K566029
location: https://synbiohub.org/public/igem/BBa_K566029/annotation2145591/range2145591/1
component: BBa_B0015

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type: Range
persistent identity: range2145591
display id: range2145591
version: 1
top level: BBa_K566029
direction: direction/reverse
start: 778
end: 906
orientation: inline

BBa_K566030
type: ComponentDefinition
persistent identity: BBa K566030
display id: BBa_K566030
version: 1
was generated by: iGEM to SBOL conversion
description: CcaS gene (constitutive expression)
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:43Z
mutable provenance: UANLBricks assambly
owned by: james
owned by: myers
mutable description: CcaS gene (constitutive expression)
discontinued: false
dominant: false
group u list: _734_
m user id: 0
owner id: 8650
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Due to illegal sites it was implemented UANLBricks assambly
star: false
creator: Daniel Rodriguez
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0015
component: BBa_K566027
component: BBa_K081005
sequence annotation: BBa_K081005
sequence annotation: BBa_K566027
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K566030_sequence/1

BBa_B0015
type: Component
persistent identity: component2145624
display id: component2145624
version: 1
top level: BBa_K566030
definition: BBa_B0015
access: public

BBa_K566027
type: Component
persistent identity: component2145617
display id: component2145617
version: 1
top level: BBa_K566030
definition: BBa_K566027
access: public

BBa_K081005
type: Component
persistent identity: component2145613
display id: component2145613
version: 1
top level: BBa_K566030
definition: BBa_K081005
access: public

BBa_K081005
type: SequenceAnnotation
persistent identity: annotation2145613
display id: annotation2145613
version: 1
top level: BBa_K566030
location: https://synbiohub.org/public/igem/BBa_K566030/annotation2145613/range2145613/1
component: BBa_K081005

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type: Range
persistent identity: range2145613
display id: range2145613
version: 1
top level: BBa_K566030
direction: direction/reverse
start: 1
end: 58
orientation: inline

BBa_K566027
type: SequenceAnnotation
persistent identity: annotation2145617
display id: annotation2145617
version: 1
top level: BBa_K566030
location: https://synbiohub.org/public/igem/BBa_K566030/annotation2145617/range2145617/1
component: BBa_K566027

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type: Range
persistent identity: range2145617
display id: range2145617
version: 1
top level: BBa_K566030
direction: direction/reverse
start: 65
end: 2326
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2145624
display id: annotation2145624
version: 1
top level: BBa_K566030
location: https://synbiohub.org/public/igem/BBa_K566030/annotation2145624/range2145624/1
component: BBa_B0015

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type: Range
persistent identity: range2145624
display id: range2145624
version: 1
top level: BBa_K566030
direction: direction/reverse
start: 2335
end: 2463
orientation: inline

BBa_K566023
type: ComponentDefinition
persistent identity: BBa K566023
display id: BBa_K566023
version: 1
was generated by: iGEM to SBOL conversion
description: pcyA gene (constitutive expression)
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:12:43Z
mutable provenance: CDS was extracted by PCR, promoter and terminator were Classic BioBricks.
owned by: james
owned by: myers
mutable description: pcyA gene transcribed from constitutive promoter.
discontinued: false
dominant: false
experience: experience/None
group u list: _734_
m user id: 0
owner id: 8650
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: For the project that this part was made, it was needed to use other Assambly Standard.
star: false
creator: Daniel Rodriguez
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0015
component: BBa_K566021
component: BBa_K081005
sequence annotation: BBa_K081005
sequence annotation: BBa_K566021
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K566023_sequence/1

BBa_B0015
type: Component
persistent identity: component2142757
display id: component2142757
version: 1
top level: BBa_K566023
definition: BBa_B0015
access: public

BBa_K566021
type: Component
persistent identity: component2142750
display id: component2142750
version: 1
top level: BBa_K566023
definition: pcyA CDS
access: public

BBa_K081005
type: Component
persistent identity: component2142748
display id: component2142748
version: 1
top level: BBa_K566023
definition: BBa_K081005
access: public

BBa_K081005
type: SequenceAnnotation
persistent identity: annotation2142748
display id: annotation2142748
version: 1
top level: BBa_K566023
location: https://synbiohub.org/public/igem/BBa_K566023/annotation2142748/range2142748/1
component: BBa_K081005

https://synbiohub.org/public/igem/BBa_K566023/annotation2142748/range2142748/1
type: Range
persistent identity: range2142748
display id: range2142748
version: 1
top level: BBa_K566023
direction: direction/reverse
start: 1
end: 58
orientation: inline

BBa_K566021
type: SequenceAnnotation
persistent identity: annotation2142750
display id: annotation2142750
version: 1
top level: BBa_K566023
location: https://synbiohub.org/public/igem/BBa_K566023/annotation2142750/range2142750/1
component: BBa_K566021

https://synbiohub.org/public/igem/BBa_K566023/annotation2142750/range2142750/1
type: Range
persistent identity: range2142750
display id: range2142750
version: 1
top level: BBa_K566023
direction: direction/reverse
start: 65
end: 811
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2142757
display id: annotation2142757
version: 1
top level: BBa_K566023
location: https://synbiohub.org/public/igem/BBa_K566023/annotation2142757/range2142757/1
component: BBa_B0015

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type: Range
persistent identity: range2142757
display id: range2142757
version: 1
top level: BBa_K566023
direction: direction/reverse
start: 820
end: 948
orientation: inline

BBa_K566024
type: ComponentDefinition
persistent identity: BBa K566024
display id: BBa_K566024
version: 1
was generated by: iGEM to SBOL conversion
description: ho1 gene (constitutive expression)
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:12:43Z
mutable provenance: CDS was extracted by PCR, promoter and terminator were Classic BioBricks.
owned by: james
owned by: myers
mutable description: ho1 gene transcribed from constitutive promoter.
discontinued: false
dominant: false
experience: experience/None
group u list: _734_
m user id: 0
owner id: 8650
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: For the project that this part was made, it was needed to use other Assambly Standard.
star: false
creator: Daniel Rodriguez
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0015
component: BBa_K566022
component: BBa_K081005
sequence annotation: BBa_K081005
sequence annotation: BBa_K566022
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K566024_sequence/1

BBa_B0015
type: Component
persistent identity: component2142771
display id: component2142771
version: 1
top level: BBa_K566024
definition: BBa_B0015
access: public

BBa_K566022
type: Component
persistent identity: component2142764
display id: component2142764
version: 1
top level: BBa_K566024
definition: ho1 CDS
access: public

BBa_K081005
type: Component
persistent identity: component2142762
display id: component2142762
version: 1
top level: BBa_K566024
definition: BBa_K081005
access: public

BBa_K081005
type: SequenceAnnotation
persistent identity: annotation2142762
display id: annotation2142762
version: 1
top level: BBa_K566024
location: https://synbiohub.org/public/igem/BBa_K566024/annotation2142762/range2142762/1
component: BBa_K081005

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type: Range
persistent identity: range2142762
display id: range2142762
version: 1
top level: BBa_K566024
direction: direction/reverse
start: 1
end: 58
orientation: inline

BBa_K566022
type: SequenceAnnotation
persistent identity: annotation2142764
display id: annotation2142764
version: 1
top level: BBa_K566024
location: https://synbiohub.org/public/igem/BBa_K566024/annotation2142764/range2142764/1
component: BBa_K566022

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type: Range
persistent identity: range2142764
display id: range2142764
version: 1
top level: BBa_K566024
direction: direction/reverse
start: 65
end: 787
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2142771
display id: annotation2142771
version: 1
top level: BBa_K566024
location: https://synbiohub.org/public/igem/BBa_K566024/annotation2142771/range2142771/1
component: BBa_B0015

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type: Range
persistent identity: range2142771
display id: range2142771
version: 1
top level: BBa_K566024
direction: direction/reverse
start: 796
end: 924
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

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type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

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type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K081005
type: ComponentDefinition
persistent identity: BBa K081005
display id: BBa_K081005
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member and RBS
created: 2008-10-17T11:00:00Z
modified: 2015-05-08T01:08:34Z
mutable provenance: Promoter: John Anderson. RBS: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
owned by: myers
owned by: james
part status: Released HQ 2013
mutable description: Constitutive promoter (strong) with RBS (strong, efficiency=0.3)
discontinued: false
dominant: true
experience: experience/None
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: We used BioBrick Standard Assembly.
star: true
creator: Lorenzo Pasotti, Paolo Magni
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0030
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_K081005_sequence/1

BBa_B0030
type: Component
persistent identity: component1981865
display id: component1981865
version: 1
top level: BBa_K081005
definition: BBa_B0030
access: public

BBa_J23100
type: Component
persistent identity: component1981863
display id: component1981863
version: 1
top level: BBa_K081005
definition: BBa_J23100
access: public

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation1981863
display id: annotation1981863
version: 1
top level: BBa_K081005
location: https://synbiohub.org/public/igem/BBa_K081005/annotation1981863/range1981863/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K081005/annotation1981863/range1981863/1
type: Range
persistent identity: range1981863
display id: range1981863
version: 1
top level: BBa_K081005
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1981865
display id: annotation1981865
version: 1
top level: BBa_K081005
location: https://synbiohub.org/public/igem/BBa_K081005/annotation1981865/range1981865/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081005/annotation1981865/range1981865/1
type: Range
persistent identity: range1981865
display id: range1981865
version: 1
top level: BBa_K081005
direction: direction/forward
start: 44
end: 58
orientation: inline

BBa_K566028
type: ComponentDefinition
persistent identity: BBa K566028
display id: BBa_K566028
version: 1
was generated by: iGEM to SBOL conversion
description: CcaR
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:43Z
mutable provenance: pJT122
owned by: james
owned by: myers
mutable description: CcaR coding sequence
discontinued: false
dominant: false
group u list: _734_
m user id: 0
owner id: 8650
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: No internal sites
star: false
creator: Daniel Rodriguez
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start
sequence annotation: CDS
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K566028_sequence/1

Start
type: SequenceAnnotation
persistent identity: annotation2145571
display id: annotation2145571
version: 1
top level: BBa_K566028
location: https://synbiohub.org/public/igem/BBa_K566028/annotation2145571/range2145571/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K566028/annotation2145571/range2145571/1
type: Range
persistent identity: range2145571
display id: range2145571
version: 1
top level: BBa_K566028
direction: direction/forward
start: 1
end: 3
orientation: inline

CDS
type: SequenceAnnotation
persistent identity: annotation2145570
display id: annotation2145570
version: 1
top level: BBa_K566028
location: https://synbiohub.org/public/igem/BBa_K566028/annotation2145570/range2145570/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K566028/annotation2145570/range2145570/1
type: Range
persistent identity: range2145570
display id: range2145570
version: 1
top level: BBa_K566028
direction: direction/forward
start: 1
end: 705
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2145569
display id: annotation2145569
version: 1
top level: BBa_K566028
location: https://synbiohub.org/public/igem/BBa_K566028/annotation2145569/range2145569/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K566028/annotation2145569/range2145569/1
type: Range
persistent identity: range2145569
display id: range2145569
version: 1
top level: BBa_K566028
direction: direction/forward
start: 703
end: 705
orientation: inline

BBa_K566027
type: ComponentDefinition
persistent identity: BBa K566027
display id: BBa_K566027
version: 1
was generated by: iGEM to SBOL conversion
description: CcaS
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:43Z
mutable provenance: pJT122
owned by: james
owned by: myers
mutable description: CcaS coding region
discontinued: false
dominant: false
group u list: _734_
m user id: 0
owner id: 8650
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: It needs UANLBricks to be managed
star: false
creator: Daniel Rodriguez
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence annotation: Start
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K566027_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2145566
display id: annotation2145566
version: 1
top level: BBa_K566027
location: https://synbiohub.org/public/igem/BBa_K566027/annotation2145566/range2145566/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K566027/annotation2145566/range2145566/1
type: Range
persistent identity: range2145566
display id: range2145566
version: 1
top level: BBa_K566027
direction: direction/forward
start: 1
end: 2262
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2145564
display id: annotation2145564
version: 1
top level: BBa_K566027
location: https://synbiohub.org/public/igem/BBa_K566027/annotation2145564/range2145564/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K566027/annotation2145564/range2145564/1
type: Range
persistent identity: range2145564
display id: range2145564
version: 1
top level: BBa_K566027
direction: direction/forward
start: 1
end: 3
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2145565
display id: annotation2145565
version: 1
top level: BBa_K566027
location: https://synbiohub.org/public/igem/BBa_K566027/annotation2145565/range2145565/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K566027/annotation2145565/range2145565/1
type: Range
persistent identity: range2145565
display id: range2145565
version: 1
top level: BBa_K566027
direction: direction/forward
start: 2260
end: 2262
orientation: inline

pcyA CDS
type: ComponentDefinition
persistent identity: BBa K566021
display id: BBa_K566021
version: 1
was generated by: iGEM to SBOL conversion
description: pcyA CDS
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:12:43Z
mutable provenance: pPLPCB(S) plasmid from Dr. Jeff Tabor
owned by: james
owned by: myers
mutable description: pcyA CDS
discontinued: false
dominant: false
experience: experience/None
group u list: _734_
m user id: 0
owner id: 8650
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: No considerations.
star: false
creator: Daniel Rodriguez
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: pcyA
sequence: https://synbiohub.org/public/igem/BBa_K566021_sequence/1

pcyA
type: SequenceAnnotation
persistent identity: annotation2142741
display id: annotation2142741
version: 1
top level: pcyA CDS
location: https://synbiohub.org/public/igem/BBa_K566021/annotation2142741/range2142741/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K566021/annotation2142741/range2142741/1
type: Range
persistent identity: range2142741
display id: range2142741
version: 1
top level: pcyA CDS
direction: direction/forward
start: 1
end: 747
orientation: inline

ho1 CDS
type: ComponentDefinition
persistent identity: BBa K566022
display id: BBa_K566022
version: 1
was generated by: iGEM to SBOL conversion
description: ho1 CDS
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:12:43Z
mutable provenance: Extracted by PCR from pPLPCB(S) plasmid of Dr. Jeff Tabor.
owned by: james
owned by: myers
mutable description: ho1 CDS
discontinued: false
dominant: false
experience: experience/None
group u list: _734_
m user id: 0
owner id: 8650
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: No considerations were needed.
star: false
creator: Daniel Rodriguez
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: ho1
sequence: https://synbiohub.org/public/igem/BBa_K566022_sequence/1

ho1
type: SequenceAnnotation
persistent identity: annotation2142743
display id: annotation2142743
version: 1
top level: ho1 CDS
location: https://synbiohub.org/public/igem/BBa_K566022/annotation2142743/range2142743/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K566022/annotation2142743/range2142743/1
type: Range
persistent identity: range2142743
display id: range2142743
version: 1
top level: ho1 CDS
direction: direction/forward
start: 1
end: 723
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

https://synbiohub.org/public/igem/BBa_K566031_sequence/1
type: Sequence
persistent identity: BBa K566031 sequence
display id: BBa_K566031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatgagaattcttttagtggaggatgatttgccgctggcggaaaccctt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K566029_sequence/1
type: Sequence
persistent identity: BBa K566029 sequence
display id: BBa_K566029_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatgagaattcttttagtggaggatgatttgccgctggcggaaacccttgctgaagcattgagtgaccagctttacaccgttgatattgccaccgacgcttccctcgcctgggactatgcctcccgactggaatatgacctcgttattttggatgtgatgctgccggagttggacgggattaccctctgtcaaaaatggcgatcgcacagttatttaatgccaattttgatgatgacagccagggatacgatcaatgataaaatcacgggcttggatgcgggggcggatgattatgtggtcaagccagtggatttgggggagttatttgccagggtgcgagctttgttgcgtcggggttgtgcaacgtgccaaccagttttagagtgggggccaatcaggttggatccaagcacctatgaagttagttatgacaatgaggttttgtctttgacccgcaaggaatacagcattctggaattactactccgcaatggccgtcgggtgctaagtcggagcatgattatcgatagtatctggaagttggagagtcccccagaggaagatacggttaaggtgcatgtgcggagtttgcgacaaaaattaaaaagtgccggtttatcagcagatgccattgaaacggtccatggcattgggtatcgtctggccaatttaacggaaaaatctttgtgccaagggaaaaactagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K566030_sequence/1
type: Sequence
persistent identity: BBa K566030 sequence
display id: BBa_K566030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatgggcaaatttctaattccaatcgaatttgtttttctggcgatcgccatgacctgttatttatggcacagacaaaaccaagaacgccgcaggattgaaattagcatcaagcaacaaacccaacgggaacgatttattaaccaaattacccaacatatccgccaatctttaaacttggaaacggttttaaataccaccgtcgctgaagttaaaaccctgttgcaagttgatcgagttctaatttatcgcatttggcaagatggcacgggcagcgccattacggaatcggtgaatgccaattatcctagtattttagggcggaccttttccgatgaagtttttcccgttgaataccatcaagcctacaccaaaggtaaagtacgggccattaatgacattgaccaggatgacatagagatttgcctagctgatttcgtcaaacaatttggcgtgaaatcaaaattagtagtgcccattcttcaacataatcgtgcttcttccctagataatgaatcagaatttccctatctttgggggctgttaattacccatcaatgtgcttttacccggccatggcaaccgtgggaagtggagttaatgaaacagctagccaatcaggtcgcgatcgccatccaacaatcggaattatatgagcaattacagcaactcaataaagatttggaaaaccgagtcgaaaaacgcacccagcaacttgccgccaccaatcaatccctaagaatggaaatcagtgagcgacaaaaaacggaagccgctctccgccacactaaccatactctgcaatccctgattgcggcctcccccaggggtatttttacccttaatttagcagaccaaattcagatttggaatcctacagcagaacgtatttttggttggacagaaacagaaattattgcccatccagaattattaacatccaacattttgctggaagattatcagcaatttaaacagaaagttttatcaggcatggtttcccctagcctagaattaaaatgtcaaaaaaaagatggtagttggattgaaattgtcctttccgctgctcccctattggatagtgaagaaaatattgccggattggtggcggttgtcgccgatattaccgagcaaaagcggcaggcagaacaaattcgtttgctacaatccgttgtggttaatactaatgatgcggtggtgattacggaagcggagcccattgatgatcccgggccgagaattctctatgtcaatgaagcatttactaaaatcaccggttatactgctgaagaaatgctaggcaaaaccccccgagttttacagggaccaaaaactagtcgcactgaattagatagggtgcggcaagccattagtcaatggcaatcagttaccgttgaagtgattaattatcgtaaggatggcagtgagttttgggtggaatttagtctggtgcccgttgccaataaaacaggtttttacacccattggattgctgtgcaaagggatgtcactgagcgccgacgcacggaggaagtccgcctagctttagaacgggaaaaagaattaagccgcctaaaaactcgttttttctccatggcttcccatgaatttcgtactcccctcagtacggccttagctgctgcccaattactggaaaattctgaagtggcctggcttgatcccgataagcgtagccggaacttacaccgtattcaaaattccgtgaaaaatatggtacagctcctggatgatattttaatcattaaccgtgccgaagcgggcaaattggaatttaatcctaattggttagatttgaaattattgttccagcaatttatcgaagaaattcaattaagtgtcagtgaccaatattattttgactttatttgtagcgctcaagatacgaaggcattggtggatgaaaggttagtgcggtcta…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K566023_sequence/1
type: Sequence
persistent identity: BBa K566023 sequence
display id: BBa_K566023_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K566024_sequence/1
type: Sequence
persistent identity: BBa K566024 sequence
display id: BBa_K566024_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K081005_sequence/1
type: Sequence
persistent identity: BBa K081005 sequence
display id: BBa_K081005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K566028_sequence/1
type: Sequence
persistent identity: BBa K566028 sequence
display id: BBa_K566028_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagaattcttttagtggaggatgatttgccgctggcggaaacccttgctgaagcattgagtgaccagctttacaccgttgatattgccaccgacgcttccctcgcctgggactatgcctcccgactggaatatgacctcgttattttggatgtgatgctgccggagttggacgggattaccctctgtcaaaaatggcgatcgcacagttatttaatgccaattttgatgatgacagccagggatacgatcaatgataaaatcacgggcttggatgcgggggcggatgattatgtggtcaagccagtggatttgggggagttatttgccagggtgcgagctttgttgcgtcggggttgtgcaacgtgccaaccagttttagagtgggggccaatcaggttggatccaagcacctatgaagttagttatgacaatgaggttttgtctttgacccgcaaggaatacagcattctggaattactactccgcaatggccgtcgggtgctaagtcggagcatgattatcgatagtatctggaagttggagagtcccccagaggaagatacggttaaggtgcatgtgcggagtttgcgacaaaaattaaaaagtgccggtttatcagcagatgccattgaaacggtccatggcattgggtatcgtctggccaatttaacggaaaaatctttgtgccaagggaaaaactag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K566027_sequence/1
type: Sequence
persistent identity: BBa K566027 sequence
display id: BBa_K566027_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgggcaaatttctaattccaatcgaatttgtttttctggcgatcgccatgacctgttatttatggcacagacaaaaccaagaacgccgcaggattgaaattagcatcaagcaacaaacccaacgggaacgatttattaaccaaattacccaacatatccgccaatctttaaacttggaaacggttttaaataccaccgtcgctgaagttaaaaccctgttgcaagttgatcgagttctaatttatcgcatttggcaagatggcacgggcagcgccattacggaatcggtgaatgccaattatcctagtattttagggcggaccttttccgatgaagtttttcccgttgaataccatcaagcctacaccaaaggtaaagtacgggccattaatgacattgaccaggatgacatagagatttgcctagctgatttcgtcaaacaatttggcgtgaaatcaaaattagtagtgcccattcttcaacataatcgtgcttcttccctagataatgaatcagaatttccctatctttgggggctgttaattacccatcaatgtgcttttacccggccatggcaaccgtgggaagtggagttaatgaaacagctagccaatcaggtcgcgatcgccatccaacaatcggaattatatgagcaattacagcaactcaataaagatttggaaaaccgagtcgaaaaacgcacccagcaacttgccgccaccaatcaatccctaagaatggaaatcagtgagcgacaaaaaacggaagccgctctccgccacactaaccatactctgcaatccctgattgcggcctcccccaggggtatttttacccttaatttagcagaccaaattcagatttggaatcctacagcagaacgtatttttggttggacagaaacagaaattattgcccatccagaattattaacatccaacattttgctggaagattatcagcaatttaaacagaaagttttatcaggcatggtttcccctagcctagaattaaaatgtcaaaaaaaagatggtagttggattgaaattgtcctttccgctgctcccctattggatagtgaagaaaatattgccggattggtggcggttgtcgccgatattaccgagcaaaagcggcaggcagaacaaattcgtttgctacaatccgttgtggttaatactaatgatgcggtggtgattacggaagcggagcccattgatgatcccgggccgagaattctctatgtcaatgaagcatttactaaaatcaccggttatactgctgaagaaatgctaggcaaaaccccccgagttttacagggaccaaaaactagtcgcactgaattagatagggtgcggcaagccattagtcaatggcaatcagttaccgttgaagtgattaattatcgtaaggatggcagtgagttttgggtggaatttagtctggtgcccgttgccaataaaacaggtttttacacccattggattgctgtgcaaagggatgtcactgagcgccgacgcacggaggaagtccgcctagctttagaacgggaaaaagaattaagccgcctaaaaactcgttttttctccatggcttcccatgaatttcgtactcccctcagtacggccttagctgctgcccaattactggaaaattctgaagtggcctggcttgatcccgataagcgtagccggaacttacaccgtattcaaaattccgtgaaaaatatggtacagctcctggatgatattttaatcattaaccgtgccgaagcgggcaaattggaatttaatcctaattggttagatttgaaattattgttccagcaatttatcgaagaaattcaattaagtgtcagtgaccaatattattttgactttatttgtagcgctcaagatacgaaggcattggtggatgaaaggttagtgcggtctattttatctaatctgttatctaatgcgattaaatactctcccgggggagggcagattaaaattgc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K566021_sequence/1
type: Sequence
persistent identity: BBa K566021 sequence
display id: BBa_K566021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K566022_sequence/1
type: Sequence
persistent identity: BBa K566022 sequence
display id: BBa_K566022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z