LOCUS BBa_K566032 8301 bp DNA linear 22-Nov-2024 DEFINITION E. coli MXRedGreen ACCESSION BBa_K566032 VERSION BBa_K566032.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 RBS 44..58 /label=RBS-1\Strong RBS 51..55 /label=RBS misc_feature 44..58 /label=BBa_B0030 RBS 44..58 /label=BBa_B0030 misc_feature 1..58 /label=BBa_K081005 CDS 65..811 /label=pcyA CDS 65..811 /label=BBa_K566021 stem_loop 831..874 /label=stem_loop misc_feature 820..899 /label=BBa_B0010 terminator 820..899 /label=BBa_B0010 stem_loop 915..934 /label=T7 TE misc_feature 941..941 /label=stop polyA_site 935..948 /label=polya misc_feature 908..948 /label=BBa_B0012 terminator 908..948 /label=BBa_B0012 terminator 820..948 /label=BBa_B0015 misc_feature 1..948 /label=BBa_K566023 promoter 957..991 /label=BBa_J23100 RBS 1000..1014 /label=RBS-1\Strong RBS 1007..1011 /label=RBS misc_feature 1000..1014 /label=BBa_B0030 RBS 1000..1014 /label=BBa_B0030 misc_feature 957..1014 /label=BBa_K081005 CDS 1021..1743 /label=ho1 CDS 1021..1743 /label=BBa_K566022 stem_loop 1763..1806 /label=stem_loop misc_feature 1752..1831 /label=BBa_B0010 terminator 1752..1831 /label=BBa_B0010 stem_loop 1847..1866 /label=T7 TE misc_feature 1873..1873 /label=stop polyA_site 1867..1880 /label=polya misc_feature 1840..1880 /label=BBa_B0012 terminator 1840..1880 /label=BBa_B0012 terminator 1752..1880 /label=BBa_B0015 misc_feature 957..1880 /label=BBa_K566024 promoter 1889..1923 /label=BBa_J23100 RBS 1932..1946 /label=RBS-1\Strong RBS 1939..1943 /label=RBS misc_feature 1932..1946 /label=BBa_B0030 RBS 1932..1946 /label=BBa_B0030 misc_feature 1889..1946 /label=BBa_K081005 misc_feature 2655..2657 /label=Stop CDS 1953..2657 /label=CDS misc_feature 1953..1955 /label=Start CDS 1953..2657 /label=BBa_K566028 stem_loop 2677..2720 /label=stem_loop misc_feature 2666..2745 /label=BBa_B0010 terminator 2666..2745 /label=BBa_B0010 stem_loop 2761..2780 /label=T7 TE misc_feature 2787..2787 /label=stop polyA_site 2781..2794 /label=polya misc_feature 2754..2794 /label=BBa_B0012 terminator 2754..2794 /label=BBa_B0012 terminator 2666..2794 /label=BBa_B0015 misc_feature 1889..2794 /label=BBa_K566029 promoter 2803..2837 /label=BBa_J23100 RBS 2846..2860 /label=RBS-1\Strong RBS 2853..2857 /label=RBS misc_feature 2846..2860 /label=BBa_B0030 RBS 2846..2860 /label=BBa_B0030 misc_feature 2803..2860 /label=BBa_K081005 misc_feature 2867..2869 /label=Start misc_feature 5126..5128 /label=Stop CDS 2867..5128 /label=cds CDS 2867..5128 /label=BBa_K566027 stem_loop 5148..5191 /label=stem_loop misc_feature 5137..5216 /label=BBa_B0010 terminator 5137..5216 /label=BBa_B0010 stem_loop 5232..5251 /label=T7 TE misc_feature 5258..5258 /label=stop polyA_site 5252..5265 /label=polya misc_feature 5225..5265 /label=BBa_B0012 terminator 5225..5265 /label=BBa_B0012 terminator 5137..5265 /label=BBa_B0015 misc_feature 2803..5265 /label=BBa_K566030 promoter 5274..5308 /label=BBa_J23100 RBS 5317..5331 /label=RBS-1\Strong RBS 5324..5328 /label=RBS misc_feature 5317..5331 /label=BBa_B0030 RBS 5317..5331 /label=BBa_B0030 misc_feature 5274..5331 /label=BBa_K081005 CDS 5338..7572 /label=Cph8 CDS 5338..7572 /label=BBa_K566025 stem_loop 7592..7635 /label=stem_loop misc_feature 7581..7660 /label=BBa_B0010 terminator 7581..7660 /label=BBa_B0010 stem_loop 7676..7695 /label=T7 TE misc_feature 7702..7702 /label=stop polyA_site 7696..7709 /label=polya misc_feature 7669..7709 /label=BBa_B0012 terminator 7669..7709 /label=BBa_B0012 terminator 7581..7709 /label=BBa_B0015 misc_feature 5274..7709 /label=BBa_K566026 misc_feature 7730..7747 /label=C1 OmpR misc_feature 7751..7768 /label=C2 OmpR misc_feature 7771..7788 /label=C3 OmpR promoter 7792..7797 /label=-35 promoter 7815..7820 /label=-10 promoter 7718..7825 /label=BBa_R0082 misc_feature 7838..7841 /label=conserved RBS 7834..7845 /label=BBa_B0034 CDS 7852..8100 /label=mnt misc_feature 7946..7948 /label=T misc_feature 8101..8133 /label=LVA misc_feature 8140..8164 /label=Help:Barcodes CDS 7852..8164 /label=BBa_C0072 stem_loop 8184..8227 /label=stem_loop misc_feature 8173..8252 /label=BBa_B0010 terminator 8173..8252 /label=BBa_B0010 stem_loop 8268..8287 /label=T7 TE misc_feature 8294..8294 /label=stop polyA_site 8288..8301 /label=polya misc_feature 8261..8301 /label=BBa_B0012 terminator 8261..8301 /label=BBa_B0012 terminator 8173..8301 /label=BBa_B0015 misc_feature 7718..8301 /label=BBa_K566020 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagattaaag aggagaaata 61 ctagatggcc gtcactgatt taagtttgac caattcttcc ctgatgccta cgttgaaccc 121 gatgattcaa cagttggccc tggcgatcgc cgctagttgg caaagtttac ccctcaagcc 181 ctatcaattg ccggaggatt tgggctacgt agaaggccgc ctggaagggg aaaagttagt 241 gattgaaaat cggtgctacc aaacgcccca gtttcgcaaa atgcatttgg agttggccaa 301 ggtgggcaaa gggttggata ttctccactg tgtaatgttt cctgagcctt tatacggtct 361 acctttgttt ggctgtgaca ttgtggccgg ccccggtgga gtaagtgcgg ctattgcgga 421 tctatccccc acccaaagcg atcgccaatt gcccgcagcg taccaaaaat cattggcaga 481 gctaggccag ccagaatttg agcaacaacg ggaattgccc ccctggggag aaatattttc 541 tgaatattgt ttattcatcc gtcccagcaa tgtcactgaa gaagaaagat ttgtacaaag 601 ggtagtggac tttttgcaaa ttcattgtca ccaatccatc gttgccgaac ccttgtctga 661 agctcaaact ttggagcacc gtcaggggca aattcattac tgccaacaac aacagaaaaa 721 tgataaaacc cgtcgggtac tggaaaaagc ttttggggaa gcttgggcgg aacggtatat 781 gagccaagtc ttatttgatg ttatccaata atactagagc caggcatcaa ataaaacgaa 841 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 901 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagttga 961 cggctagctc agtcctaggt acagtgctag ctactagaga ttaaagagga gaaatactag 1021 atgagtgtca acttagcttc ccagttgcgg gaagggacga aaaaatccca ctccatggcg 1081 gagaacgtcg gctttgtcaa atgcttcctc aagggcgttg tcgagaaaaa ttcctaccgt 1141 aagctggttg gcaatctcta ctttgtctac agtgccatgg aagaggaaat ggcaaaattt 1201 aaggaccatc ccatcctcag ccacatttac ttccccgaac tcaaccgcaa acaaagccta 1261 gagcaagacc tgcaattcta ttacggctcc aactggcggc aagaagtgaa aatttctgcc 1321 gctggccaag cctatgtgga ccgagtccgg caagtggccg ctacggcccc tgaattgttg 1381 gtggcccatt cctacacccg ttacctgggg gatctttccg gcggtcaaat tctcaagaaa 1441 attgcccaaa atgccatgaa tctccacgat ggtggcacag ctttctatga atttgccgac 1501 attgatgacg aaaaggcttt taaaaatacc taccgtcaag ctatgaatga tctgcccatt 1561 gaccaagcca ccgccgaacg gattgtggat gaagccaatg acgcctttgc catgaacatg 1621 aaaatgttca acgaacttga aggcaacctg atcaaggcga tcggcattat ggtgttcaac 1681 agcctcaccc gtcgccgcag tcaaggcagc accgaagttg gcctcgccac ctccgaaggc 1741 tagtactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 1801 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 1861 tgggcctttc tgcgtttata tactagagtt gacggctagc tcagtcctag gtacagtgct 1921 agctactaga gattaaagag gagaaatact agatgagaat tcttttagtg gaggatgatt 1981 tgccgctggc ggaaaccctt gctgaagcat tgagtgacca gctttacacc gttgatattg 2041 ccaccgacgc ttccctcgcc tgggactatg cctcccgact ggaatatgac ctcgttattt 2101 tggatgtgat gctgccggag ttggacggga ttaccctctg tcaaaaatgg cgatcgcaca 2161 gttatttaat gccaattttg atgatgacag ccagggatac gatcaatgat aaaatcacgg 2221 gcttggatgc gggggcggat gattatgtgg tcaagccagt ggatttgggg gagttatttg 2281 ccagggtgcg agctttgttg cgtcggggtt gtgcaacgtg ccaaccagtt ttagagtggg 2341 ggccaatcag gttggatcca agcacctatg aagttagtta tgacaatgag gttttgtctt 2401 tgacccgcaa ggaatacagc attctggaat tactactccg caatggccgt cgggtgctaa 2461 gtcggagcat gattatcgat agtatctgga agttggagag tcccccagag gaagatacgg 2521 ttaaggtgca tgtgcggagt ttgcgacaaa aattaaaaag tgccggttta tcagcagatg 2581 ccattgaaac ggtccatggc attgggtatc gtctggccaa tttaacggaa aaatctttgt 2641 gccaagggaa aaactagtac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 2701 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 2761 ggctcacctt cgggtgggcc tttctgcgtt tatatactag agttgacggc tagctcagtc 2821 ctaggtacag tgctagctac tagagattaa agaggagaaa tactagatgg gcaaatttct 2881 aattccaatc gaatttgttt ttctggcgat cgccatgacc tgttatttat ggcacagaca 2941 aaaccaagaa cgccgcagga ttgaaattag catcaagcaa caaacccaac gggaacgatt 3001 tattaaccaa attacccaac atatccgcca atctttaaac ttggaaacgg ttttaaatac 3061 caccgtcgct gaagttaaaa ccctgttgca agttgatcga gttctaattt atcgcatttg 3121 gcaagatggc acgggcagcg ccattacgga atcggtgaat gccaattatc ctagtatttt 3181 agggcggacc ttttccgatg aagtttttcc cgttgaatac catcaagcct acaccaaagg 3241 taaagtacgg gccattaatg acattgacca ggatgacata gagatttgcc tagctgattt 3301 cgtcaaacaa tttggcgtga aatcaaaatt agtagtgccc attcttcaac ataatcgtgc 3361 ttcttcccta gataatgaat cagaatttcc ctatctttgg gggctgttaa ttacccatca 3421 atgtgctttt acccggccat ggcaaccgtg ggaagtggag ttaatgaaac agctagccaa 3481 tcaggtcgcg atcgccatcc aacaatcgga attatatgag caattacagc aactcaataa 3541 agatttggaa aaccgagtcg aaaaacgcac ccagcaactt gccgccacca atcaatccct 3601 aagaatggaa atcagtgagc gacaaaaaac ggaagccgct ctccgccaca ctaaccatac 3661 tctgcaatcc ctgattgcgg cctcccccag gggtattttt acccttaatt tagcagacca 3721 aattcagatt tggaatccta cagcagaacg tatttttggt tggacagaaa cagaaattat 3781 tgcccatcca gaattattaa catccaacat tttgctggaa gattatcagc aatttaaaca 3841 gaaagtttta tcaggcatgg tttcccctag cctagaatta aaatgtcaaa aaaaagatgg 3901 tagttggatt gaaattgtcc tttccgctgc tcccctattg gatagtgaag aaaatattgc 3961 cggattggtg gcggttgtcg ccgatattac cgagcaaaag cggcaggcag aacaaattcg 4021 tttgctacaa tccgttgtgg ttaatactaa tgatgcggtg gtgattacgg aagcggagcc 4081 cattgatgat cccgggccga gaattctcta tgtcaatgaa gcatttacta aaatcaccgg 4141 ttatactgct gaagaaatgc taggcaaaac cccccgagtt ttacagggac caaaaactag 4201 tcgcactgaa ttagataggg tgcggcaagc cattagtcaa tggcaatcag ttaccgttga 4261 agtgattaat tatcgtaagg atggcagtga gttttgggtg gaatttagtc tggtgcccgt 4321 tgccaataaa acaggttttt acacccattg gattgctgtg caaagggatg tcactgagcg 4381 ccgacgcacg gaggaagtcc gcctagcttt agaacgggaa aaagaattaa gccgcctaaa 4441 aactcgtttt ttctccatgg cttcccatga atttcgtact cccctcagta cggccttagc 4501 tgctgcccaa ttactggaaa attctgaagt ggcctggctt gatcccgata agcgtagccg 4561 gaacttacac cgtattcaaa attccgtgaa aaatatggta cagctcctgg atgatatttt 4621 aatcattaac cgtgccgaag cgggcaaatt ggaatttaat cctaattggt tagatttgaa 4681 attattgttc cagcaattta tcgaagaaat tcaattaagt gtcagtgacc aatattattt 4741 tgactttatt tgtagcgctc aagatacgaa ggcattggtg gatgaaaggt tagtgcggtc 4801 tattttatct aatctgttat ctaatgcgat taaatactct cccgggggag ggcagattaa 4861 aattgcccta agcctagatt cggaacagat tatttttgaa gtcaccgacc agggcattgg 4921 catttcgcca gaggaccaaa agcaaatttt tgaacccttt catcggggca aaaatgtcag 4981 aaatattacg ggaacaggac tcggtttaat ggttgccaag aaatgtgttg acttacacag 5041 tggcagtatc ttgctaaaaa gtgcagttga ccagggaaca acagttacta tctgtttaaa 5101 acgctataac catttgcctc gagcttagta ctagagccag gcatcaaata aaacgaaagg 5161 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 5221 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagttgacgg 5281 ctagctcagt cctaggtaca gtgctagcta ctagagatta aagaggagaa atactagatg 5341 gccaccaccg tacaactcag cgaccaatcc ctccgtcagc tagaaaccct cgccatccac 5401 accgcccacc tgattcagcc ccacggttta gtggtggtcc tgcaggaacc agacctcacc 5461 atcagccaaa ttagcgccaa ctgcaccggc attttagggc gatcgccaga ggatttgttg 5521 ggcagaaccc taggggaagt gtttgatagc tttcagattg atcccatcca gagtcgccta 5581 acggccggac aaatcagcag cctcaacccc agtaaacttt gggcgcgggt catgggggac 5641 gactttgtca tttttgacgg ggtttttcat cgcaacagtg acggtttatt ggtatgtgaa 5701 ctagagccag cctacacttc cgataatctg cccttcctcg gtttttatca catggccaac 5761 gctgccctga atcggttgcg ccaacaagct aatctacggg atttctacga tgttattgtc 5821 gaagaagtcc gccgtatgac tggctttgac cgggtgatgc tataccgctt tgatgaaaat 5881 aaccacggtg atgtcattgc cgaagataaa cgggatgata tggaacccta tttgggcctg 5941 cactatcccg aatcggatat tccccaaccc gcccgtcggc tatttatcca caaccccatt 6001 cgagtaattc ccgatgttta tggtgtggcg gtgcccctga ccccagcggt taaccccagc 6061 accaaccgag cggtggattt aacagaatcc attctgcgca gtgcgtacca ttgccacttg 6121 acctatctga aaaatatggg ggtaggagcg tctttaacca tttccctaat taaggacggc 6181 catctctggg ggctcattgc ctgccaccat caaaccccca aagtaattcc ctttgaactg 6241 cgtaaagcct gcgaattttt tggtcgggtg gtgtttagca acatttccgc ccaggaagat 6301 acggaaacct tcgattaccg ggtgcagttg gcggagcatg aagcggtttt attggacaaa 6361 atgaccacgg cggcggattt tgtcgaagga ttaactaatc atcccgatcg cctgttggga 6421 ttaacgggct cccagggggc ggccatttgc tttggggaaa aattgatttt agtaggggaa 6481 accccggacg agaaagcagt gcaatattta ctgcaatggt tggagaatcg ggaagtgcaa 6541 gacgttttct tcacctcttc cctctcacaa atttatcctg atgcagtgaa ttttaaatcc 6601 gtggccagtg gcttattggc cattcccatt gcccgtcaca actttttgct ctggtttcgc 6661 cctgaagtgt tgcaaacggt taattggggc ggtgacccaa atcatgctta cgaagctacc 6721 caggaagacg gtaaaatcga gctccatccc cgccaatcct ttgacctctg gaaagaaatt 6781 gtccgactcc aatctttgcc ctggcaatcg gtggaaatcc aaagtgccct ggccctgaaa 6841 aaggcgatcg tcaacctcat tttgcgccag gcagaagaat tgcatatggc ggctggtgtt 6901 aagcaactgg cggatgaccg cacgctgctg atggcggggg taagtcacga cttgcgcacg 6961 ccgctgacgc gtattcgcct ggcgactgag atgatgagcg agcaggatgg ctatctggca 7021 gaatcgatca ataaagatat cgaagagtgc aacgccatca ttgagcagtt tatcgactac 7081 ctgcgcaccg ggcaggagat gccgatggaa atggcggatc ttaatgcagt actcggtgag 7141 gtgattgctg ccgaaagtgg ctatgagcgg gaaattgaaa ccgcgcttta ccccggcagc 7201 attgaagtga aaatgcaccc gctgtcgatc aaacgcgcgg tggcgaatat ggtggtcaac 7261 gccgcccgtt atggcaatgg ctggatcaaa gtcagcagcg gaacggagcc gaatcgcgcc 7321 tggttccagg tggaagatga cggtccggga attgcgccgg aacaacgtaa gcacctgttc 7381 cagccgtttg tccgcggcga cagtgcgcgc accattagcg gcacgggatt agggctggca 7441 attgtgcagc gtatcgtgga taaccataac gggatgctgg agcttggcac cagcgagcgg 7501 ggcgggcttt ccattcgcgc ctggctgcca gtgccggtaa cgcgggcgca gggcatgaca 7561 aaagaagggt aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 7621 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc 7681 accttcgggt gggcctttct gcgtttatat actagagtcc cttgcattta cattttgaaa 7741 catctatagc gataaatgaa acatcttaaa agttttagta tcatattcgt gttggattat 7801 tctgcatttt tggggagaat ggacttacta gagaaagagg agaaatacta gatggcccgg 7861 gatgatcctc acttcaattt tcgtatgcca atggaagtaa gagagaaatt gaaatttaga 7921 gcagaggcaa acggacggag catgaactct gagcttttgc aaatcgtaca agatgcccta 7981 agcaaaccgt caccagtcac tgggtaccgc aatgatgcgg aacgactcgc cgatgagcag 8041 agcgagttag tgaagaagat ggtcttcgat acactgaagg atctttataa aaaaaccacc 8101 gctgcaaacg acgaaaacta cgctttagta gcttaataac cctgatagtg ctagtgtaga 8161 tccctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 8221 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 8281 gtgggccttt ctgcgtttat a //