LOCUS       BBa_K566040              971 bp    DNA     linear       27-Dec-2024
DEFINITION  pRM434-RFP with double terminator
ACCESSION   BBa_K566040
VERSION     BBa_K566040.1  
FEATURES             Location/Qualifiers
     misc_feature    56..69
                     /label=OR1 434
     misc_feature    9..25
                     /label=OR1 lambda
     misc_feature    33..49
                     /label=OR2 lambda
     promoter        71..76
                     /label=-10
     promoter        48..53
                     /label=-35
     promoter        1..82
                     /label=BBa_I12006
     RBS             91..105
                     /label=RBS-1\Strong
     RBS             98..102
                     /label=RBS
     misc_feature    91..105
                     /label=BBa_B0030
     RBS             91..105
                     /label=BBa_B0030
     CDS             112..819
                     /label=mCherry (RFP)
     misc_feature    820..828
                     /label=LVA
     misc_feature    112..834
                     /label=BBa_K566013
     misc_feature    1..834
                     /label=BBa_K566017
     stem_loop       854..897
                     /label=stem_loop
     misc_feature    843..922
                     /label=BBa_B0010
     terminator      843..922
                     /label=BBa_B0010
     stem_loop       938..957
                     /label=T7 TE
     misc_feature    964..964
                     /label=stop
     polyA_site      958..971
                     /label=polya
     misc_feature    931..971
                     /label=BBa_B0012
     terminator      931..971
                     /label=BBa_B0012
     terminator      843..971
                     /label=BBa_B0015
ORIGIN
        1 gcaaccatta tcaccgccag aggtaaaata gtcaacacgc acggtgttag atattacaaa
       61 ctttcttgta tagatttaac gttactagag attaaagagg agaaatacta gatggttagc
      121 aaaggtgaag aggataacat ggccatcatc aaagagttca tgcgctttaa agttcacatg
      181 gaaggtagcg ttaatggcca cgaatttgaa attgaaggtg aaggcgaagg tcgtccgtat
      241 gaaggcaccc agaccgcaaa actgaaagtt accaaaggtg gtccgctgcc gtttgcatgg
      301 gatattctga gtccgcagtt tatgtatggt agcaaagcct atgttaaaca tccggcagat
      361 atcccggatt atctgaaact gagctttccg gaaggtttta aatgggaacg tgtgatgaat
      421 tttgaagatg gtggtgttgt taccgttacc caggatagca gcctgcaaga tggtgaattt
      481 atctataaag ttaaactgcg tggcaccaat tttccgagtg atggtccggt tatgcagaaa
      541 aaaaccatgg gttgggaagc aagcagcgaa cgtatgtatc cggaagatgg cgcactgaaa
      601 ggtgaaatta aacagcgcct gaaactgaaa gatggcggtc attatgatgc agaagttaaa
      661 accacctata aagccaaaaa accggttcag ctgcctggtg catataacgt taacattaaa
      721 ctggatatca ccagccacaa cgaggattat accattgttg aacagtatga acgtgcagaa
      781 ggtcgccata gtaccggtgg tatggatgaa ctgtataaac tggttgcctg ataatactag
      841 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
      901 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
      961 ctgcgtttat a
//