LOCUS       BBa_K566042             2690 bp    DNA     linear       28-Dec-2024
DEFINITION  composite Biphasic switch
ACCESSION   BBa_K566042
VERSION     BBa_K566042.1  
FEATURES             Location/Qualifiers
     promoter        1..85
                     /label=pRM
     promoter        1..85
                     /label=BBa_K566001
     misc_feature    98..101
                     /label=conserved
     RBS             94..105
                     /label=BBa_B0034
     CDS             112..780
                     /label=cI434
     misc_feature    742..780
                     /label=LVA
     CDS             112..780
                     /label=BBa_K566011
     misc_feature    793..796
                     /label=conserved
     RBS             789..800
                     /label=BBa_B0034
     misc_feature    1..800
                     /label=BBa_K566015
     misc_feature    807..809
                     /label=start
     misc_feature    1560..1562
                     /label=stop
     misc_feature    1563..1565
                     /label=stop
     misc_feature    1523..1559
                     /label=LVA tag
     CDS             810..1522
                     /label=cds
     misc_feature    807..1565
                     /label=BBa_K145015
     stem_loop       1581..1600
                     /label=T7 TE
     misc_feature    1607..1607
                     /label=stop
     polyA_site      1601..1614
                     /label=polya
     misc_feature    1574..1614
                     /label=BBa_B0012
     terminator      1574..1614
                     /label=BBa_B0012
     stem_loop       1635..1657
                     /label=stem_loop
     misc_feature    1623..1668
                     /label=BBa_B0011
     terminator      1623..1668
                     /label=BBa_B0011
     terminator      1574..1668
                     /label=BBa_B0014
     CDS             1677..2426
                     /label=cI
     CDS             1677..2426
                     /label=BBa_K566038
     RBS             2435..2447
                     /label=RBS 3.0
     RBS             2435..2447
                     /label=BBa_K566037
     promoter        2456..2553
                     /label=pTet/Mnt Hybrid Promoter (Inverted)
     promoter        2456..2553
                     /label=BBa_K566036
     misc_feature    2562..2690
                     /label=OL (inverted)
     promoter        2562..2690
                     /label=BBa_K566035
     misc_feature    1677..2690
                     /label=BBa_K566039
ORIGIN
        1 catgcaacca ttatcaccgc cagaggtaaa atagtcaaca cgcacggtgt tagatattta
       61 tcccttgcgg tgatagattt aacgttacta gagaaagagg agaaatacta gatgagcatt
      121 agcagccgtg ttaaaagcaa acgtattcag ctgggtctga atcaggcaga actggcacag
      181 aaagttggca ccacccagca gagcattgaa cagctggaaa atggtaaaac caaacgtccg
      241 cgttttctgc cggaactggc aagcgcactg ggtgttagcg ttgattggct gctgaatggc
      301 accagcgata gcaatgttcg ttttgttggt catgttgaac cgaaaggtaa atatccgctg
      361 attagcatgg ttcgtgcagg tagctggtgt gaagcatgtg aaccgtatga tatcaaagat
      421 atcgatgagt ggtatgactc cgatgttaat ctgctgggca atggtttttg gctgaaagtt
      481 gaaggtgata gcatgaccag tccggttggt cagagcattc cggaaggtca tatggttctg
      541 gttgataccg gtcgtgaacc ggttaatggt agcctggttg ttgcaaaact gaccgatgca
      601 aatgaagcca ccttcaaaaa actggttatt gatggtggtc agaaatacct gaaaggcctg
      661 aatccgagct ggccgatgac cccgattaat ggtaattgca aaattatcgg tgttgtggtt
      721 gaagcccgtg tgaaatttgt tgcagcaaac gatgaaaatt atgccctggt tgcctaataa
      781 tactagagaa agaggagaaa tactagatgc gtaaaggaga agaacttttc actggagttg
      841 tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag
      901 agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa
      961 aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg
     1021 cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt
     1081 atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag
     1141 tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag
     1201 aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca
     1261 tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg
     1321 aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc
     1381 ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca
     1441 acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg
     1501 gcatggatga actatacaaa aggcctgctg caaacgacga aaactacgct ttagtagctt
     1561 aataatacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag
     1621 agagagaata taaaaagcca gattattaat ccggcttttt tattatttta ctagagttat
     1681 caggccacca gtgcataatt ttcatcattt gctgcaccaa aggtttcttc aggccactgg
     1741 cttgcaataa ctttacccac aacgctacag ctttcattac acggaatcat cggatactgc
     1801 ggattcagcg gttgcagaaa aacctgaccg ctatcacgaa tcagtttttt gaaggtgaac
     1861 tcatcaccac ccagacgtgc aatacaaaaa tcacccggtt caactgcctg ttccggatca
     1921 accagaatca gcataccatc cggaaagctc ggtttgctac cggtcggtgc ggtcatgcta
     1981 ttaccttcaa cttccagcca aaatgcgcta tcgcttgctt ttttggttgt gctaacccaa
     2041 cgttctgcat cacctttggt aaaggtacgc agttccggac taaacatacc tgcctgaaca
     2101 tggctaaaaa ccggatattc atattcgcta cgcaggctcg gctgcatgct aacggcttca
     2161 tacatttcat agatttcacg tgcaatgctc gggctgaact cttcaacgct aactttcaga
     2221 atttttgcca gcagtgctgc attataggca ttcagtgcat taataccatt aaacagtgca
     2281 ccaacaccgc tctgacccat acccatttta tctgcaacgc tttcttggct caggcccagt
     2341 tcattttttt ttttctcgta gattgctttc agacgacgtg catcttccag ctgttcttgg
     2401 gtcagcggtt tttttttggt gctcattact agagctttcc tgtgtgatac tagagatctc
     2461 tatcactgat agggattaca gttctctatc actgataggg aactatagga gatctaggtc
     2521 accgtggacc tagtactgtg cactcacctc gagtactaga gagtatcacc gccagtggta
     2581 tttatgtcaa caccgccaga gataatttat caccgcagat ggttatctgt atgtttttta
     2641 tatgaattta ttttttgcag gggggcattg tttggtaggt gagagatctg
//