BBa_B0014 1 BBa_B0014 double terminator (B0012-B0011) 2003-07-15T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0012 and BBa_B0011 false true _1_ 0 24 7 In stock false true Reshma Shetty component939303 1 BBa_B0012 component939311 1 BBa_B0011 annotation939311 1 BBa_B0011 range939311 1 50 95 annotation939303 1 BBa_B0012 range939303 1 1 41 BBa_K566035 1 OL Inv OL region from Lambda (Inverted) 2011-09-27T11:00:00Z 2015-05-08T01:12:43Z Synthetic DNA Inverted of the OL region from Lambda phage (K566000) false false _734_ 0 8657 9 Not in stock false Search for illegal sites was performed and it was optimized for E.coli codon usage false Jos?? Eduardo Almeyda Carbajal annotation2146514 1 OL (inverted) range2146514 1 1 129 BBa_K566001 1 pRM pRM promoter from Lambda 2011-09-25T11:00:00Z 2015-05-08T01:12:42Z It comes directly from genomic DNA of Lambda phage. It was extracted by PCR as recommended by the Registry. pRM promoter from Lambda phage. It may be positive and negative regulated according to cI protein concentration. It needs OL region in order to achieve better negative regulation. It may be used to establish a biphasic switch which could permit activation and repression of genes transcribed from it. false false _734_ 0 8650 9 Not in stock false Analysis of illegal restriction sites was performed, but it does not have them originally. false Daniel Rodriguez, Eduardo Almeyda, Porfirio Quintero annotation2145891 1 pRM range2145891 1 1 85 BBa_K566042 1 BBa_K566042 composite Biphasic switch 2011-09-27T11:00:00Z 2015-05-08T01:12:43Z Planning Composite part of BBa_K566015, BBa_K145015, BBa_B0014 and BBa_K566039 false false _734_ 0 8657 9 Not in stock false Search for illegal sites and it was optimized for E.coli codon usage false Porfirio Quintero-Cadena, Jos?? Eduardo Almeyda Carbajal component2146598 1 BBa_K566015 component2146604 1 BBa_K145015 component2146611 1 BBa_B0014 component2146620 1 BBa_K566039 annotation2146604 1 BBa_K145015 range2146604 1 807 1565 annotation2146598 1 BBa_K566015 range2146598 1 1 800 annotation2146611 1 BBa_B0014 range2146611 1 1574 1668 annotation2146620 1 BBa_K566039 range2146620 1 1677 2690 BBa_K566015 1 BBa_K566015 pRM-RBS-cI434 2011-09-25T11:00:00Z 2015-05-08T01:12:43Z Synthesized DNA. Composite part for Biphasic switch tests. false false _734_ 0 8650 9 It's complicated true Search for illegal sites was performed, they were found and deleted. false Daniel Rodriguez component2146403 1 BBa_B0034 component2146406 1 BBa_K566011 component2146401 1 BBa_K566001 component2146408 1 BBa_B0034 annotation2146406 1 BBa_K566011 range2146406 1 112 780 annotation2146408 1 BBa_B0034 range2146408 1 789 800 annotation2146403 1 BBa_B0034 range2146403 1 94 105 annotation2146401 1 BBa_K566001 range2146401 1 1 85 BBa_K145015 1 GFP GFP with LVA tag 2008-07-31T11:00:00Z 2015-05-08T01:10:28Z pJBA111, carrying GFP-LVA, was generously provided by Dr. S. Molin, The Technical University of Denmark, Lyngby Appl Environ Microbiol. 1998 Jun;64(6):2240-6. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Andersen JB, Sternberg C, Poulsen LK, Bjorn SP, Givskov M, Molin S. PMID: 9603842 Green Fluorescent Protein with LVA tag for rapid degradation false false _257_ 0 2939 9 It's complicated true Designed out of pJBA111 (Molin, 1998) with PCR to add appropriote prefix and suffix. true Benjamien Moeyaert annotation1969620 1 cds range1969620 1 4 716 annotation1969619 1 LVA tag range1969619 1 717 753 annotation1969618 1 stop range1969618 1 757 759 annotation1969617 1 stop range1969617 1 754 756 annotation1969616 1 start range1969616 1 1 3 BBa_K566036 1 pTet/Mnt I pTet/Mnt Hybrid Promoter (Inverted) 2011-09-27T11:00:00Z 2015-05-08T01:12:43Z Synthetic DNA Inverted version of the Mnt promoter false false _734_ 0 8657 9 Not in stock false A search for illegal sites was performed and it was optimized for E. coli codon usage false Jos?? Eduardo Almeyda Carbajal annotation2146515 1 pTet/Mnt Hybrid Promoter (Inverted) range2146515 1 1 98 BBa_B0011 1 BBa_B0011 LuxICDABEG (+/-) 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>. Released HQ 2013 Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p> false false _1_ 0 24 7 In stock false <P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A-&gt;G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P> true Reshma Shetty annotation7019 1 BBa_B0011 range7019 1 1 46 annotation1683 1 stem_loop range1683 1 13 35 BBa_K566038 1 cI Inv cI repressor from Lambda phage optimized for E. coli cI +LVA (Inverted) 2011-09-27T11:00:00Z 2015-05-08T01:12:43Z Synthetic DNA Inverted version of the cI repressor (K566012) false false _734_ 0 8657 9 Not in stock false A search for illegal sites was performed and it was optimized for E. coli codon usage false Jos?? Eduardo Almeyda Carbajal annotation2146521 1 cI range2146521 1 1 750 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_K566037 1 RBS.3 Inv RBS.3 (Inverted) 2011-09-27T11:00:00Z 2015-05-08T01:12:43Z Synthetic DNA Inverted version of the RBS.3 (BBa_B0032) false false _734_ 0 8657 9 Not in stock false A search for illegal sites was performed and it was optimized for E. coli codon usage false Jos?? Eduardo Almeyda Carbajal annotation2146517 1 RBS 3.0 range2146517 1 1 13 BBa_K566011 1 cI434 opt cI434 repressor from phage 434 optimized for E. coli cI (+LVA) 2011-09-25T11:00:00Z 2015-05-08T01:12:43Z Sequence was taken from BioBrick BBa_C0052 cI434 repressor from phage 434 optimized for E. coli cI (+LVA) false false _734_ 0 8650 9 Not in stock false Search for illegal sites was performed, they were found and deleted. false Daniel Rodriguez annotation2142580 1 LVA range2142580 1 631 669 annotation2142579 1 cI434 range2142579 1 1 669 BBa_K566039 1 BBa_K566039 OL-pTetMnt-cI (Inverted) 2011-09-27T11:00:00Z 2015-05-08T01:12:43Z Synthetic DNA Inverted version of OL-pTetMnt-cI composited part (BBa_K566016) false false _734_ 0 8657 9 Not in stock false Search for illegal sites was performed and it was optimized for E.coli codon usage false Jos?? Eduardo Almeyda Carbajal, Daniel Rodriguez component2146528 1 BBa_K566036 component2146526 1 BBa_K566037 component2146530 1 BBa_K566035 component2146524 1 BBa_K566038 annotation2146528 1 BBa_K566036 range2146528 1 780 877 annotation2146524 1 BBa_K566038 range2146524 1 1 750 annotation2146530 1 BBa_K566035 range2146530 1 886 1014 annotation2146526 1 BBa_K566037 range2146526 1 759 771 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0014_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_B0034_sequence 1 aaagaggagaaa BBa_K566035_sequence 1 agtatcaccgccagtggtatttatgtcaacaccgccagagataatttatcaccgcagatggttatctgtatgttttttatatgaatttattttttgcaggggggcattgtttggtaggtgagagatctg BBa_K566037_sequence 1 ctttcctgtgtga BBa_B0011_sequence 1 agagaatataaaaagccagattattaatccggcttttttattattt BBa_K145015_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa BBa_K566042_sequence 1 catgcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttatcccttgcggtgatagatttaacgttactagagaaagaggagaaatactagatgagcattagcagccgtgttaaaagcaaacgtattcagctgggtctgaatcaggcagaactggcacagaaagttggcaccacccagcagagcattgaacagctggaaaatggtaaaaccaaacgtccgcgttttctgccggaactggcaagcgcactgggtgttagcgttgattggctgctgaatggcaccagcgatagcaatgttcgttttgttggtcatgttgaaccgaaaggtaaatatccgctgattagcatggttcgtgcaggtagctggtgtgaagcatgtgaaccgtatgatatcaaagatatcgatgagtggtatgactccgatgttaatctgctgggcaatggtttttggctgaaagttgaaggtgatagcatgaccagtccggttggtcagagcattccggaaggtcatatggttctggttgataccggtcgtgaaccggttaatggtagcctggttgttgcaaaactgaccgatgcaaatgaagccaccttcaaaaaactggttattgatggtggtcagaaatacctgaaaggcctgaatccgagctggccgatgaccccgattaatggtaattgcaaaattatcggtgttgtggttgaagcccgtgtgaaatttgttgcagcaaacgatgaaaattatgccctggttgcctaataatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagttatcaggccaccagtgcataattttcatcatttgctgcaccaaaggtttcttcaggccactggcttgcaataactttacccacaacgctacagctttcattacacggaatcatcggatactgcggattcagcggttgcagaaaaacctgaccgctatcacgaatcagttttttgaaggtgaactcatcaccacccagacgtgcaatacaaaaatcacccggttcaactgcctgttccggatcaaccagaatcagcataccatccggaaagctcggtttgctaccggtcggtgcggtcatgctattaccttcaacttccagccaaaatgcgctatcgcttgcttttttggttgtgctaacccaacgttctgcatcacctttggtaaaggtacgcagttccggactaaacatacctgcctgaacatggctaaaaaccggatattcatattcgctacgcaggctcggctgcatgctaacggcttcatacatttcatagatttcacgtgcaatgctcgggctgaactcttcaacgctaactttcagaatttttgccagcagtgctgcattataggcattcagtgcattaataccattaaacagtgcaccaacaccgctctgacccatacccattttatctgcaacgctttcttggctcaggcccagttcatttttttttttctcgtagattgctttcagacgacgtgcatcttccagctgttcttgggtcagcggtttttttttggtgctcattactagagctttcctgtgtgatactagagatctctatcactgatagggattacagttctctatcactgatagggaactataggagatctaggtcaccgtggacctagtactgtgcactcacctcgagtactagagagtatcaccgccagtggtatttatgtcaacaccgccagagataatttatcaccgcagatggttatctgtatgttttttatatgaatttattttttgcaggggggcattgtttggtaggtgagagatctg BBa_K566039_sequence 1 ttatcaggccaccagtgcataattttcatcatttgctgcaccaaaggtttcttcaggccactggcttgcaataactttacccacaacgctacagctttcattacacggaatcatcggatactgcggattcagcggttgcagaaaaacctgaccgctatcacgaatcagttttttgaaggtgaactcatcaccacccagacgtgcaatacaaaaatcacccggttcaactgcctgttccggatcaaccagaatcagcataccatccggaaagctcggtttgctaccggtcggtgcggtcatgctattaccttcaacttccagccaaaatgcgctatcgcttgcttttttggttgtgctaacccaacgttctgcatcacctttggtaaaggtacgcagttccggactaaacatacctgcctgaacatggctaaaaaccggatattcatattcgctacgcaggctcggctgcatgctaacggcttcatacatttcatagatttcacgtgcaatgctcgggctgaactcttcaacgctaactttcagaatttttgccagcagtgctgcattataggcattcagtgcattaataccattaaacagtgcaccaacaccgctctgacccatacccattttatctgcaacgctttcttggctcaggcccagttcatttttttttttctcgtagattgctttcagacgacgtgcatcttccagctgttcttgggtcagcggtttttttttggtgctcattactagagctttcctgtgtgatactagagatctctatcactgatagggattacagttctctatcactgatagggaactataggagatctaggtcaccgtggacctagtactgtgcactcacctcgagtactagagagtatcaccgccagtggtatttatgtcaacaccgccagagataatttatcaccgcagatggttatctgtatgttttttatatgaatttattttttgcaggggggcattgtttggtaggtgagagatctg BBa_K566036_sequence 1 atctctatcactgatagggattacagttctctatcactgatagggaactataggagatctaggtcaccgtggacctagtactgtgcactcacctcgag BBa_K566011_sequence 1 atgagcattagcagccgtgttaaaagcaaacgtattcagctgggtctgaatcaggcagaactggcacagaaagttggcaccacccagcagagcattgaacagctggaaaatggtaaaaccaaacgtccgcgttttctgccggaactggcaagcgcactgggtgttagcgttgattggctgctgaatggcaccagcgatagcaatgttcgttttgttggtcatgttgaaccgaaaggtaaatatccgctgattagcatggttcgtgcaggtagctggtgtgaagcatgtgaaccgtatgatatcaaagatatcgatgagtggtatgactccgatgttaatctgctgggcaatggtttttggctgaaagttgaaggtgatagcatgaccagtccggttggtcagagcattccggaaggtcatatggttctggttgataccggtcgtgaaccggttaatggtagcctggttgttgcaaaactgaccgatgcaaatgaagccaccttcaaaaaactggttattgatggtggtcagaaatacctgaaaggcctgaatccgagctggccgatgaccccgattaatggtaattgcaaaattatcggtgttgtggttgaagcccgtgtgaaatttgttgcagcaaacgatgaaaattatgccctggttgcctaataa BBa_K566001_sequence 1 catgcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttatcccttgcggtgatagatttaacgt BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K566038_sequence 1 ttatcaggccaccagtgcataattttcatcatttgctgcaccaaaggtttcttcaggccactggcttgcaataactttacccacaacgctacagctttcattacacggaatcatcggatactgcggattcagcggttgcagaaaaacctgaccgctatcacgaatcagttttttgaaggtgaactcatcaccacccagacgtgcaatacaaaaatcacccggttcaactgcctgttccggatcaaccagaatcagcataccatccggaaagctcggtttgctaccggtcggtgcggtcatgctattaccttcaacttccagccaaaatgcgctatcgcttgcttttttggttgtgctaacccaacgttctgcatcacctttggtaaaggtacgcagttccggactaaacatacctgcctgaacatggctaaaaaccggatattcatattcgctacgcaggctcggctgcatgctaacggcttcatacatttcatagatttcacgtgcaatgctcgggctgaactcttcaacgctaactttcagaatttttgccagcagtgctgcattataggcattcagtgcattaataccattaaacagtgcaccaacaccgctctgacccatacccattttatctgcaacgctttcttggctcaggcccagttcatttttttttttctcgtagattgctttcagacgacgtgcatcttccagctgttcttgggtcagcggtttttttttggtgctcat BBa_K566015_sequence 1 catgcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttatcccttgcggtgatagatttaacgttactagagaaagaggagaaatactagatgagcattagcagccgtgttaaaagcaaacgtattcagctgggtctgaatcaggcagaactggcacagaaagttggcaccacccagcagagcattgaacagctggaaaatggtaaaaccaaacgtccgcgttttctgccggaactggcaagcgcactgggtgttagcgttgattggctgctgaatggcaccagcgatagcaatgttcgttttgttggtcatgttgaaccgaaaggtaaatatccgctgattagcatggttcgtgcaggtagctggtgtgaagcatgtgaaccgtatgatatcaaagatatcgatgagtggtatgactccgatgttaatctgctgggcaatggtttttggctgaaagttgaaggtgatagcatgaccagtccggttggtcagagcattccggaaggtcatatggttctggttgataccggtcgtgaaccggttaatggtagcctggttgttgcaaaactgaccgatgcaaatgaagccaccttcaaaaaactggttattgatggtggtcagaaatacctgaaaggcctgaatccgagctggccgatgaccccgattaatggtaattgcaaaattatcggtgttgtggttgaagcccgtgtgaaatttgttgcagcaaacgatgaaaattatgccctggttgcctaataatactagagaaagaggagaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z