BBa_K567011 1 BBa_K567011 PT7-TDRS 2011-09-28T11:00:00Z 2015-05-08T01:12:43Z aspartyl aminoacyl tRNA synthetase derived from AspS in E.coli aspartyl aminoacyl tRNA synthetase without anticodon recognition domain under the control of T7 promoter and lac operator. This biobrick is constructed by deleting the anticodon recognition domain of AspRS from E.coli. This modified AspRS can charge Asp to tRNA(Asp)-TAG (BBa_K567013) and tRNA(Asp)-AGG (BBa_K567012). false false _735_ 0 8782 9 It's complicated true Use Dicovery Studio to analyze the enzyme and find the tRNA anticodon recognition domain of AspS. false Bin Zhao annotation2150825 1 rbs range2150825 1 75 81 annotation2152822 1 AspRS(truncated) range2152822 1 89 1549 annotation2150810 1 lac operator range2150810 1 21 44 annotation2150824 1 T7 promoter range2150824 1 2 18 annotation2152823 1 T7 TE range2152823 1 1719 1766 BBa_K567011_sequence 1 ttaatacgactcactataggggaattgtgagcggataacaattcccctctagaaataattttgtttaactttaagaaggagatataccatggatgttctgccgcttgactctaaccacgtcaacaccgaagaagcgcgtctgaaataccgctacctcgacctgcgtcgtccggaaatggctcagcgcctgaaaacccgcgctaaaatcaccagcctggtgcgccgttttatggatgaccacggcttcctcgacatcgaaactccgatgctgaccaaagccacgccggaaggcgcgcgtgactacctggtgccttctcgtgtgcacaaaggtaaattctacgcactgccgcaatccccgcagttgttcaaacagctgctgatgatgtccggttttgaccgttactatcagatcgttaaatgcttccgtgacgaagacctgcgtgctgaccgtcagcctgaatttactcagatcgatgtggaaacttctttcatgaccgcgccgcaagtgcgtgaagtgatggaagcgctggtgcgtcatctgtggctggaagtgaagggtgtggatctgggcgatttcccggtaatgacctttgcggaagcagaacgccgttatggttctgataaaccggatctgcgtaacccgatggaactgactgacgttgctgatctgctgaaatctgttgagtttgctgtatttgcaggtccggcgaacgatccgaaaggtcgcgtagcggctctgcgcgttccgggcggcgcatcgctgacccgtaagcagatcgacgaatacggtaacttcgttaaaatctacggcgcgaaaggtctggcttacatcaaagttaacgaacgcgcgaaaggtctggaaggtatcaacagcccggtagcgaagttccttaatgcagaaatcatcgaagacatcctggatcgtactgccgcgcaagatggcgatatgattttcttcggtgccgacaacaagaaaattgttgccgacgcgatgggtgcactgcgcctgaaagtgggtaaagaccttggtctgaccgacgaaagcaaatgggcaccgctgtgggttatcgacttcccgatgtttgaagacgacggtgaaggcggcctgacggcaatgcaccatccgttcacctcaccgaaagatatgacggctgcagaactgaaagctgcaccggaaaatgcggtggcgaacgcttacgatatggtcatcaatggttacgaagtgggcggtggttcagtacgtatccataatggtgatatgcagcagacggtgtttggtattctgggtatcaacgaagaggaacagcgcgagaaattcggcttcctgctcgacgctctgaaatacggtactccgccgcacgcaggtctggcattcggtcttgaccgtctgaccatgctgctgaccggcaccgacaatatccgtgacgttatcgccttcccgaaaaccacggcggcagcgtgtctgatgactgaagcaccgagctttgctaacccgactgcactggctgagctgagcattcaggttgtgaagaaggctgagaataactgacatatggctagcatgactggtggacagcaaatgggtcgcggatccgaattcgagctccgtcgacaagcttgcggccgcactcgagcaccaccaccaccaccactgagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttggaattccgg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z