LOCUS       BBa_K567014             2265 bp    DNA     linear       28-Dec-2024
DEFINITION  PT7-metGM
ACCESSION   BBa_K567014
VERSION     BBa_K567014.1  
FEATURES             Location/Qualifiers
     promoter        2..18
                     /label=T7 promoter
     misc_feature    21..44
                     /label=lac operator
     RBS             75..81
                     /label=rbs
     CDS             89..2122
                     /label=MetRS(mutated)
     stem_loop       2209..2256
                     /label=T7 TE
ORIGIN
        1 ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt
       61 ttgtttaact ttaagaagga gatataccat gactcaagtc gcgaagaaaa ttctggtgac
      121 gtgcgcactg ccgtacgcta acggctcaat ccacctcggc catatgctgg agcacatcca
      181 ggctgatgtc tgggtccgtt accagcgaat gcgcggccac gaggtcaact tcatctgcgc
      241 cgacgatgcc cacggtacac cgatcatgct gaaagctcag cagcttggta tcaccccgga
      301 gcagatgatt ggcgaaatga gtcaggagca tcagactgat ttcgcaggct ttaacatcag
      361 ctatgacaac tatcactcga cgcacagcga agagaaccgc cagttgtcag aacttatcta
      421 ctctcgcctg aaagaaaacg gttttattaa aaaccgcacc atctctcagc tgtacgatcc
      481 ggaaaaaggc atgttcctgc cggaccgttt tgtgaaaggc acctgcccga aatgtaaatc
      541 cccggatcaa tacggcgata actgcgaagt ctgcggcgcg acctacagcc cgactgaact
      601 gatcgagccg aaatcggtgg tttctggcgc tacgccggta atgcgtgatt ctgaacactt
      661 cttctttgat ctgccctctt tcagcgaaat gttgcaggca tggacccgca gcggtgcgtt
      721 gcaggagcag gtggcaaata aaatgcagga gtggtttgaa tctggcctgc aacagtggga
      781 tatctcccgc gacgcccctt acttcggttt tgaaattccg aacgcgccgg gcaaatattt
      841 ctacgtctgg ctggacgcac cgattggcta catgggttct ttcaagaatc tgtgcgacaa
      901 gcgcggcgac agcgtaagct tcgatgaata ctggaagaaa gactccaccg ccgagctgta
      961 ccacttcatc ggtaaagata ttgtttactt ccacagcctg ttctggcctg ccatgctgga
     1021 aggcagcaac ttccgcaagc cgtccaacct gtttgttcat ggctatgtga cggtgaacgg
     1081 cgcaaagatg tccaagtctc gcggcacctt tattaaagcc agcacctggc tgaatcattt
     1141 tgacgcagac agcctgcgtt actactacac tgcgaaactc tcttcgcgca ttgatgatat
     1201 cgatctcaac ctggaagatt tcgttcagcg tgtgaatgcc gatatcgtta acaaagtggt
     1261 taacctggcc tcccgtaatg cgggctttat caacaagcgt tttgacggcg tgctggcaag
     1321 cgaactggct gacccgcagt tgtacaaaac cttcactgat gccgctgaag tgattggtga
     1381 agcgtgggaa agccgtgaat ttggtaaagc cgtgcgcgaa atcatggcgc tggctgatct
     1441 ggctaaccgc tatgtcgatg aacaggctcc gtgggtggtg gcgaaacagg aaggccgcga
     1501 tgccgacctg caggcaattt gctcaatggg catcaacctg ttccgcgtgc tgatgactta
     1561 cctgaagccg gtactgccga aactgaccga gcgtgcagaa gcattcctca atacggaact
     1621 gacctgggat ggtatccagc aaccgctgct gggccacaaa gtgaatccgt tcaaggcgct
     1681 gtataaccgc atcgatatga ggcaggttga agcactggtg gaagcctcta aagaagaagt
     1741 aaaagccgct gccgcgccgg taactggccc gctggcagat gatccgattc aggaaaccat
     1801 cacctttgac gacttcgcta aagttgacct gcgcgtggcg ctgattgaaa acgcagagtt
     1861 tgttgaaggt tctgacaaac tgctgcgcct gacgctggat ctcggcggtg aaaaacgcaa
     1921 tgtcttctcc ggtattcgtt ctgcttaccc ggatccgcag gcactgattg gtcgtcacac
     1981 cattatggtg gctaacctgg caccacgtaa aatgcgcttc ggtatctctg aaggcatggt
     2041 gatggctgcc ggtcctggcg ggaaagatat tttcctgcta agcccggatg ccggtgctaa
     2101 accgggtcat caggtgaaat aaggcaccac caccaccacc actgagatcc ggctgctaac
     2161 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc
     2221 cttggggcct ctaaacgggt cttgaggggt tttttggaat tccgg
//