LOCUS       BBa_K567015             1445 bp    DNA     linear       22-Nov-2024
DEFINITION  PT7-metGN
ACCESSION   BBa_K567015
VERSION     BBa_K567015.1  
FEATURES             Location/Qualifiers
     promoter        2..18
                     /label=T7 promoter
     misc_feature    21..44
                     /label=lac operator
     RBS             75..81
                     /label=rbs
     CDS             89..1219
                     /label=MetRS(truncated)
     stem_loop       1389..1436
                     /label=T7 TE
ORIGIN
        1 ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt
       61 ttgtttaact ttaagaagga gatataccat gactcaagtc gcgaagaaaa ttctggtgac
      121 gtgcgcactg ccgtacgcta acggctcaat ccacctcggc catatgctgg agcacatcca
      181 ggctgatgtc tgggtccgtt accagcgaat gcgcggccac gaggtcaact tcatctgcgc
      241 cgacgatgcc cacggtacac cgatcatgct gaaagctcag cagcttggta tcaccccgga
      301 gcagatgatt ggcgaaatga gtcaggagca tcagactgat ttcgcaggct ttaacatcag
      361 ctatgacaac tatcactcga cgcacagcga agagaaccgc cagttgtcag aacttatcta
      421 ctctcgcctg aaagaaaacg gttttattaa aaaccgcacc atctctcagc tgtacgatcc
      481 ggaaaaaggc atgttcctgc cggaccgttt tgtgaaaggc acctgcccga aatgtaaatc
      541 cccggatcaa tacggcgata actgcgaagt ctgcggcgcg acctacagcc cgactgaact
      601 gatcgagccg aaatcggtgg tttctggcgc tacgccggta atgcgtgatt ctgaacactt
      661 cttctttgat ctgccctctt tcagcgaaat gttgcaggca tggacccgca gcggtgcgtt
      721 gcaggagcag gtggcaaata aaatgcagga gtggtttgaa tctggcctgc aacagtggga
      781 tatctcccgc gacgcccctt acttcggttt tgaaattccg aacgcgccgg gcaaatattt
      841 ctacgtctgg ctggacgcac cgattggcta catgggttct ttcaagaatc tgtgcgacaa
      901 gcgcggcgac agcgtaagct tcgatgaata ctggaagaaa gactccaccg ccgagctgta
      961 ccacttcatc ggtaaagata ttgtttactt ccacagcctg ttctggcctg ccatgctgga
     1021 aggcagcaac ttccgcaagc cgtccaacct gtttgttcat ggctatgtga cggtgaacgg
     1081 cgcaaagatg tccaagtctc gcggcacctt tattaaagcc agcacctggc tgaatcattt
     1141 tgacgcagac agcctgcgtt actactacac tgcgaaactc tcttcgcgca ttgatgatat
     1201 cgatctcaac ctcgagtaac atatggctag catgactggt ggacagcaaa tgggtcgcgg
     1261 atccgaattc gagctccgtc gacaagcttg cggccgcact cgagcaccac caccaccacc
     1321 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
     1381 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttggaat
     1441 tccgg
//