LOCUS       BBa_K567018             1768 bp    DNA     linear       25-Nov-2024
DEFINITION  PT7-GFP-TAG-RFP
ACCESSION   BBa_K567018
VERSION     BBa_K567018.1  
FEATURES             Location/Qualifiers
     promoter        2..18
                     /label=PT7
     misc_feature    21..44
                     /label=lac operator
     RBS             75..81
                     /label=rbs
     misc_feature    197..910
                     /label=GFP
     misc_feature    926..928
                     /label=TAG
     misc_feature    944..1621
                     /label=mRFP
     CDS             89..1621
                     /label=GFP-TAG-RFP
     stem_loop       1712..1759
                     /label=T7 TE
ORIGIN
        1 ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt
       61 ttgtttaact ttaagaagga gatataccat gggcagcagc catcatcatc atcatcacag
      121 cagcggcctg gtgccgcgcg gcagccatat ggctagcatg actggtggac agcaaatggg
      181 tcgcggatcc gaattcatgc gtaaaggaga agaacttttc actggagttg tcccaattct
      241 tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg
      301 tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt
      361 tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc
      421 agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga
      481 aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga
      541 aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa
      601 cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga
      661 caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag
      721 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt
      781 accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag
      841 agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga
      901 actatacaaa ggtggtggtg gttcataggg aggaggagga agtatggctt cctccgaaga
      961 cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta acggtcacga
     1021 gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact
     1081 gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc cgcagttcca
     1141 gtacggttcc aaagcttacg ttaaacaccc ggctgacatc ccggactacc tgaaactgtc
     1201 cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg gtgttgttac
     1261 cgttacccag gactcctccc tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg
     1321 taccaacttc ccgtccgacg gtccggttat gcagaaaaaa accatgggtt gggaagcttc
     1381 caccgaacgt atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa
     1441 actgaaagac ggtggtcact acgacgctga agttaaaacc acctacatgg ctaaaaaacc
     1501 ggttcagctg ccgggtgctt acaaaaccga catcaaactg gacatcacct cccacaacga
     1561 agactacacc atcgttgaac agtacgaacg tgctgaaggt cgtcactcca ccggtgctta
     1621 actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc
     1681 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
     1741 ggtcttgagg ggttttttgg aattccgg
//