LOCUS       BBa_K567023             2043 bp    DNA     linear       15-Jun-2024
DEFINITION  PT7-Luc-4AGG-tRNA(Arg)
ACCESSION   BBa_K567023
VERSION     BBa_K567023.1  
FEATURES             Location/Qualifiers
     promoter        2..18
                     /label=PT7
     misc_feature    21..44
                     /label=lac operator
     RBS             75..81
                     /label=rbs
     misc_feature    89..91
                     /label=ATG
     CDS             89..1762
                     /label=luciferase
     misc_feature    95..106
                     /label=4AGG
     misc_feature    1869..1943
                     /label=tRNA(Arg)
ORIGIN
        1 ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt
       61 ttgtttaact ttaagaagga gatataccat ggcgaggagg aggagggcgg cggaagacgc
      121 caaaaacata aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga
      181 gcaactgcat aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga
      241 tgcacatatc gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc
      301 agaagctatg aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa
      361 ctctcttcaa ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc
      421 cgcgaacgac atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac
      481 cgtggtgttc gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc
      541 aatcatccaa aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat
      601 gtacacgttc gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga
      661 gtccttcgat agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct
      721 gcctaaaggt gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccagaga
      781 tcctattttt ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca
      841 tcacggtttt ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt
      901 aatgtataga tttgaagaag agctgtttct gaggagcctt caggattaca agattcaaag
      961 tgcgctgctg gtgccaaccc tattctcctt cttcgccaaa agcactctga ttgacaaata
     1021 cgatttatct aatttacacg aaattgcttc tggtggcgct cccctctcta aggaagtcgg
     1081 ggaagcggtt gccaagaggt tccatctgcc aggtatcagg caaggatatg ggctcactga
     1141 gactacatca gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa
     1201 agttgttcca ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt
     1261 taatcaaaga ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa
     1321 tccggaagcg accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc
     1381 ttactgggac gaagacgaac acttcttcat cgttgaccgc ctgaagtctc tgattaagta
     1441 caaaggctat caggtggctc ccgctgaatt ggaatccatc ttgctccaac accccaacat
     1501 cttcgacgca ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt
     1561 tgttgttttg gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag
     1621 tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa
     1681 aggtcttacc ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa
     1741 gggcggaaag atcgccgtgt aacatatgga aaaattataa aaacccggca taaatggcga
     1801 gggtttaagc aatcgagcgg cagcgtactt accccgcact ccattagcgg gtatactcat
     1861 gccgcattgt cctcttagtt aaatggatat aacgagcccc tcctaagggc taattgcagg
     1921 ttcgattcct gcaggggaca ccatttatca gttcgctccc atccgtacca gtccgcaaaa
     1981 tcccctgaat atcaagcatt ccgtagattt acagttcgtc atggttcgct tcagatcgtt
     2041 gac
//