LOCUS BBa_K568001 6853 bp DNA linear 22-Dec-2024 DEFINITION Optogenetical AND-Gate red/blue light ACCESSION BBa_K568001 VERSION BBa_K568001.1 FEATURES Location/Qualifiers promoter 1..35 /label=J23110 RBS 43..54 /label=B0032 CDS 63..629 /label=Heme oxygenase RBS 630..644 /label=rbs CDS 650..1396 /label=pcyA misc_feature 1..1399 /label=BBa_K322123 RBS 1413..1417 /label=RBS CDS 1423..3657 /label=Cph8 misc_feature 1408..3660 /label=BBa_K322124 misc_feature 3681..3698 /label=C1 OmpR misc_feature 3702..3719 /label=C2 OmpR misc_feature 3722..3739 /label=C3 OmpR promoter 3743..3748 /label=-35 promoter 3766..3771 /label=-10 promoter 3669..3776 /label=BBa_R0082 misc_feature 3785..3920 /label=BBa_K228001 stem_loop 3940..3983 /label=stem_loop misc_feature 3929..4008 /label=BBa_B0010 terminator 3929..4008 /label=BBa_B0010 stem_loop 4024..4043 /label=T7 TE misc_feature 4050..4050 /label=stop polyA_site 4044..4057 /label=polya misc_feature 4017..4057 /label=BBa_B0012 terminator 4017..4057 /label=BBa_B0012 terminator 3929..4057 /label=BBa_B0015 promoter 4093..4098 /label=-35 box promoter 4117..4123 /label=-10 box misc_feature 4099..4116 /label=spacer misc_feature 4118..4124 /label=inverted repeat part 1 misc_feature 4132..4138 /label=inverted repeat part 2 misc_feature 4129..4130 /label=transcription start promoter 4066..4151 /label=BBa_K238013 RBS 4160..4174 /label=Reverse RBS RBS 4160..4174 /label=BBa_J44001 CDS 4181..6853 /label=cds CDS 4181..6853 /label=BBa_K228000 ORIGIN 1 tttacggcta gctcagtcct aggtattatg ctagctacta gagtcacaca ggaaaggtgc 61 acatggaaga ggaaatggca aaatttaagg accatcccat cctcagccac atttacttcc 121 ccgaactcaa ccgcaaacaa agcctagagc aagacctgca attctattac ggctccaact 181 ggcggcaaga agtgaaaatt tctgccgctg gccaagccta tgtggaccga gtccggcaag 241 tggccgctac ggcccctgaa ttgttggtgg cccattccta cacccgttac ctgggggatc 301 tttccggcgg tcaaattctc aagaaaattg cccaaaatgc catgaatctc cacgatggtg 361 gcacagcttt ctatgaattt gccgacattg atgacgaaaa ggcttttaaa aatacctacc 421 gtcaagctat gaatgatctg cccattgacc aagccaccgc cgaacggatt gtggatgaag 481 ccaatgacgc ctttgccatg aacatgaaaa tgttcaacga acttgaaggc aacctgatca 541 aggcgatcgg cattatggtg ttcaacagcc tcacccgtcg ccgcagtcaa ggcagcaccg 601 aagttggcct cgccacctcc gaaggctagt taaagaggag aaaggatcca tggccgtcac 661 tgatttaagt ttgaccaatt cttccctgat gcctacgttg aacccgatga ttcaacagtt 721 ggccctggcg atcgccgcta gttggcaaag tttacccctc aagccctatc aattgccgga 781 ggatttgggc tacgtagaag gccgcctgga aggggaaaag ttagtgattg aaaatcggtg 841 ctaccaaacg ccccagtttc gcaaaatgca tttggagttg gccaaggtgg gcaaagggtt 901 ggatattctc cactgtgtaa tgtttcctga gcctttatac ggtctacctt tgtttggctg 961 tgacattgtg gccggccccg gtggagtaag tgcggctatt gcggatctat cccccaccca 1021 aagcgatcgc caattgcccg cagcgtacca aaaatcattg gcagagctag gccagccaga 1081 atttgagcaa caacgggaat tgcccccctg gggagaaata ttttctgaat attgtttatt 1141 catccgtccc agcaatgtca ctgaagaaga aagatttgta caaagggtag tggacttttt 1201 gcaaattcat tgtcaccaat ccatcgttgc cgaacccttg tctgaagctc aaactttgga 1261 gcaccgtcag gggcaaattc attactgcca acaacaacag aaaaatgata aaacccgtcg 1321 ggtactggaa aaagcttttg gggaagcttg ggcggaacgg tatatgagcc aagtcttatt 1381 tgatgttatc caataataat actagagagg agaaaggtac ccatggccac caccgtacaa 1441 ctcagcgacc aatccctccg tcagctagaa accctcgcca tccacaccgc ccacctgatt 1501 cagccccacg gtttagtggt ggtcctgcaa gaaccagacc tcaccatcag ccaaattagc 1561 gccaactgca ccggcatttt agggcgatcg ccagaggatt tgttgggcag aaccctaggg 1621 gaagtgtttg atagctttca gattgatccc atccagagtc gcctaacggc cggacaaatc 1681 agcagcctca accccagtaa actttgggcg cgggtcatgg gggacgactt tgtcattttt 1741 gacggggttt ttcatcgcaa cagtgacggt ttattggtat gtgaactcga gccagcctac 1801 acttccgata atctgccctt cctcggtttt tatcacatgg ccaacgctgc cctgaatcgg 1861 ttgcgccaac aagctaatct acgggatttc tacgatgtta ttgtcgaaga agtccgccgt 1921 atgactggct ttgaccgggt gatgctatac cgctttgatg aaaataacca cggtgatgtc 1981 attgccgaag ataaacggga tgatatggaa ccctatttgg gcctgcacta tcccgaatcg 2041 gatattcccc aacccgcccg tcggctattt atccacaacc ccattcgagt aattcccgat 2101 gtttatggtg tggcggtgcc cctgacccca gcggttaacc ccagcaccaa ccgagcggtg 2161 gatttaacag aatccattct gcgcagtgcg taccattgcc acttgaccta tctgaaaaat 2221 atgggggtag gagcgtcttt aaccatttcc ctaattaagg acggccatct ctgggggctc 2281 attgcctgcc accatcaaac ccccaaagta attccctttg aactgcgtaa agcctgcgaa 2341 ttttttggtc gggtggtgtt tagcaacatt tccgcccagg aagatacgga aaccttcgat 2401 taccgggtgc agctggcgga gcatgaagcg gttttattgg acaaaatgac cacggcggcg 2461 gattttgtcg aaggattaac taatcatccc gatcgcctgt tgggattaac gggctcccag 2521 ggggcggcca tttgctttgg ggaaaaattg attttagtag gggaaacccc ggacgagaaa 2581 gcagtgcaat atttactgca atggttggag aatcgggaag tgcaagacgt tttcttcacc 2641 tcttccctct cacaaattta tcctgatgca gtgaatttta aatccgtggc cagtggctta 2701 ttggccattc ccattgcccg tcacaacttt ttgctctggt ttcgccctga agtgttgcaa 2761 acggttaatt ggggcggtga cccaaatcat gcttacgaag ctacccagga agacggtaaa 2821 atcgagctcc atccccgcca atcctttgac ctctggaaag aaattgtccg actccaatct 2881 ttgccctggc aatcggtgga aatccaaagt gccctggccc tgaaaaaggc gatcgtcaac 2941 ctcattttgc gccaggcaga agaattgcat atggcggctg gtgttaagca actggcggat 3001 gaccgcacgc tgctgatggc gggggtaagt cacgacttgc gcacgccgct gacgcgtatt 3061 cgcctggcga ctgagatgat gagcgagcag gatggctatc tggcagaatc gatcaataaa 3121 gatatcgaag agtgcaacgc catcattgag cagtttatcg actacctgcg caccgggcag 3181 gagatgccga tggaaatggc ggatcttaat gcagtactcg gtgaggtgat tgctgccgaa 3241 agtggctatg agcgggaaat tgaaaccgcg ctttaccccg gcagcattga agtgaaaatg 3301 cacccgctgt cgatcaaacg cgcggtggcg aatatggtgg tcaacgccgc ccgttatggc 3361 aatggctggg tcaaagtcag cagcggaacg gagccgaatc gcgcctggtt ccaggtggaa 3421 gatgacggtc cgggaattgc gccggaacaa cgtaagcacc tgttccagcc gtttgtccgc 3481 ggcgacagtg cgcgcaccat tagcggcacg ggattagggc tggcaattgt gcagcgtatc 3541 gtggataacc ataacgggat gctggagctt ggcaccagcg agcggggcgg gctttccatt 3601 cgcgcctggc tgccagtgcc ggtaacgcgg gcgcagggca tgacaaaaga agggtaataa 3661 tactagagtc ccttgcattt acattttgaa acatctatag cgataaatga aacatcttaa 3721 aagttttagt atcatattcg tgttggatta ttctgcattt ttggggagaa tggacttact 3781 agagcaattc ggagagatgc cggagcggct gaacggaccg gtctctaaaa ccggagtagg 3841 ggcaactcta ccgggggttc aaatccccct ctctccgcca ctacagatcc ttagcgaaag 3901 ctaaggattt tttttaagct tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 3961 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 4021 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagattgc aaaaaattaa 4081 tttatcattc tgtacacata tttcgtacaa gtttgctatt gttacttcac ttaacattga 4141 ttaacatttt ttactagagt ttctcctctt taattactag atgaccatga ttaccgtgca 4201 ctagaatacc attaacattg ctaagaacga cttctctgac atcgaactgg ctgctatccc 4261 gttcaacact ctggctgacc attacggtga gcgtttagct cgcgaacagt tggcccttga 4321 gcatgagtct tacgagatgg gtgaagcacg cttccgcaag atgtttgagc gtcaacttaa 4381 agctggtgag gttgcggata acgctgccgc caagcctctc atcactaccc tactccctaa 4441 gatgattgca cgcatcaacg actggtttga ggaagtgaaa gctaagcgcg gcaagcgccc 4501 gacagccttc cagttcctgt aggaaatcaa gccggaagcc gtagcgtaca tcaccattaa 4561 gaccactctg gcttgcctaa ccagtgctga caatacaacc gttcaggctg tagcaagcgc 4621 aatcggtcgg gccattgagg acgaggctcg cttcggtcgt atccgtgacc ttgaagctaa 4681 gcacttcaag aaaaacgttg aggaacaact caacaagcgc gtagggcacg tctacaagaa 4741 agcatttatg caagttgtcg aggctgacat gctctctaag ggtctactcg gtggcgaggc 4801 gtggtcttcg tggcataagg aagactctat tcatgtagga gtacgctgca tcgagatgct 4861 cattgagtca accggaatgg ttagcttaca ccgccaaaat gctggcgtag taggtcaaga 4921 ctctgagact atcgaactcg cacctgaata cgctgaggct atcgcaaccc gtgcaggtgc 4981 gctggctggc atctctccga tgttccaacc ttgcgtagtt cctcctaagc cgtggactgg 5041 cattactggt ggtggctatt gggctaacgg tcgtcgtcct ctggcgctgg tgcgtactca 5101 cagtaagaaa gcactgatgc gctacgaaga cgtttacatg cctgaggtgt acaaagcgat 5161 taacattgcg caaaacaccg catggaaaat caacaagaaa gtcctagcgg tcgccaacgt 5221 aatcaccaag tggaagcatt gtccggtcga ggacatccct gcgattgagc gtgaagaact 5281 cccgatgaaa ccggaagaca tcgacatgaa tcctgaggct ctcaccgcgt ggaaacgtgc 5341 tgccgctgct gtgtaccgca aggacaaggc tcgcaagtct cgccgtatca gccttgagtt 5401 catgcttgag caagccaata agtttgctaa ccataaggcc atctggttcc cttacaacat 5461 ggactggcgc ggtcgtgttt acgctgtgtc aatgttcaac ccgcaaggta acgatatgac 5521 caaaggactg cttacgctgg cgaaaggtaa accaatcggt aaggaaggtt actactggct 5581 gaaaatccac ggtgcaaact gtgcgggtgt cgataaggtt ccgttccctg agcgcatcaa 5641 gttcattgag gaaaaccacg agaacatcat ggcttgcgct aagtctccac tggagaacac 5701 ttggtgggct gagcaagatt ctccgttctg cttccttgcg ttctgctttg agtacgctgg 5761 ggtacagcac cacggcctga gctataactg ctcccttccg ctggcgtttg acgggtcttg 5821 ctctggcatc cagcacttct ccgcgatgct ccgagatgag gtaggtggtc gcgcggttaa 5881 cttgcttcct agtgaaaccg ttcaggacat ctacgggatt gttgctaaga aagtcaacga 5941 gattctacaa gcagacgcaa tcaatgggac cgataacgaa gtagttaccg tgaccgatga 6001 gaacactggt gaaatctctg agaaagtcaa gctgggcact aaggcactgg ctggtcaatg 6061 gctggcttac ggtgttactc gcagtgtgac taagcgttca gtcatgacgc tggcttacgg 6121 gtccaaagag ttcggcttcc gtcaacaagt gctggaagat accattcagc cagctattga 6181 ttccggcaag ggtctgatgt tcactcagcc gaatcaggct gctggataca tggctaagct 6241 gatttgggaa tctgtgagcg tgacggtggt agctgcggtt gaagcaatga actggcttaa 6301 gtctgctgct aagctgctgg ctgctgaggt caaagataag aagactggag agattcttcg 6361 caagcgttgc gctgtgcatt gggtaactcc tgatggtttc cctgtgtggc aggaatacaa 6421 gaagcctatt cagacgcgct tgaacctgat gttcctcggt cagttccgct tacagcctac 6481 cattaacacc aacaaagata gcgagattga tgcacacaaa caggagtctg gtatcgctcc 6541 taactttgta cacagccaag acggtagcca ccttcgtaag actgtagtgt gggcacacga 6601 gaagtacgga atcgaatctt ttgcactgat tcacgactcc ttcggtacca ttccggctga 6661 cgctgcgaac ctgttcaaag cagtgcgcga aactatggtt gacacatatg agtcttgtga 6721 tgtactggct gatttctacg accagttcgc tgaccagttg cacgagtctc aattggacaa 6781 aatgccagca cttccggcta aaggtaactt gaacctccgt gacatcttag agtcggactt 6841 cgcgttcgca taa //