LOCUS BBa_K568004 3069 bp DNA linear 22-Dec-2024 DEFINITION Optogenetical AND-Gate blue light - without first promoter - choose your first input ACCESSION BBa_K568004 VERSION BBa_K568004.1 FEATURES Location/Qualifiers misc_feature 1..136 /label=BBa_K228001 stem_loop 156..199 /label=stem_loop misc_feature 145..224 /label=BBa_B0010 terminator 145..224 /label=BBa_B0010 stem_loop 240..259 /label=T7 TE misc_feature 266..266 /label=stop polyA_site 260..273 /label=polya misc_feature 233..273 /label=BBa_B0012 terminator 233..273 /label=BBa_B0012 terminator 145..273 /label=BBa_B0015 promoter 309..314 /label=-35 box promoter 333..339 /label=-10 box misc_feature 315..332 /label=spacer misc_feature 334..340 /label=inverted repeat part 1 misc_feature 348..354 /label=inverted repeat part 2 misc_feature 345..346 /label=transcription start promoter 282..367 /label=BBa_K238013 RBS 376..390 /label=Reverse RBS RBS 376..390 /label=BBa_J44001 CDS 397..3069 /label=cds CDS 397..3069 /label=BBa_K228000 ORIGIN 1 caattcggag agatgccgga gcggctgaac ggaccggtct ctaaaaccgg agtaggggca 61 actctaccgg gggttcaaat ccccctctct ccgccactac agatccttag cgaaagctaa 121 ggattttttt taagcttact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 181 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 241 gctcaccttc gggtgggcct ttctgcgttt atatactaga gattgcaaaa aattaattta 301 tcattctgta cacatatttc gtacaagttt gctattgtta cttcacttaa cattgattaa 361 cattttttac tagagtttct cctctttaat tactagatga ccatgattac cgtgcactag 421 aataccatta acattgctaa gaacgacttc tctgacatcg aactggctgc tatcccgttc 481 aacactctgg ctgaccatta cggtgagcgt ttagctcgcg aacagttggc ccttgagcat 541 gagtcttacg agatgggtga agcacgcttc cgcaagatgt ttgagcgtca acttaaagct 601 ggtgaggttg cggataacgc tgccgccaag cctctcatca ctaccctact ccctaagatg 661 attgcacgca tcaacgactg gtttgaggaa gtgaaagcta agcgcggcaa gcgcccgaca 721 gccttccagt tcctgtagga aatcaagccg gaagccgtag cgtacatcac cattaagacc 781 actctggctt gcctaaccag tgctgacaat acaaccgttc aggctgtagc aagcgcaatc 841 ggtcgggcca ttgaggacga ggctcgcttc ggtcgtatcc gtgaccttga agctaagcac 901 ttcaagaaaa acgttgagga acaactcaac aagcgcgtag ggcacgtcta caagaaagca 961 tttatgcaag ttgtcgaggc tgacatgctc tctaagggtc tactcggtgg cgaggcgtgg 1021 tcttcgtggc ataaggaaga ctctattcat gtaggagtac gctgcatcga gatgctcatt 1081 gagtcaaccg gaatggttag cttacaccgc caaaatgctg gcgtagtagg tcaagactct 1141 gagactatcg aactcgcacc tgaatacgct gaggctatcg caacccgtgc aggtgcgctg 1201 gctggcatct ctccgatgtt ccaaccttgc gtagttcctc ctaagccgtg gactggcatt 1261 actggtggtg gctattgggc taacggtcgt cgtcctctgg cgctggtgcg tactcacagt 1321 aagaaagcac tgatgcgcta cgaagacgtt tacatgcctg aggtgtacaa agcgattaac 1381 attgcgcaaa acaccgcatg gaaaatcaac aagaaagtcc tagcggtcgc caacgtaatc 1441 accaagtgga agcattgtcc ggtcgaggac atccctgcga ttgagcgtga agaactcccg 1501 atgaaaccgg aagacatcga catgaatcct gaggctctca ccgcgtggaa acgtgctgcc 1561 gctgctgtgt accgcaagga caaggctcgc aagtctcgcc gtatcagcct tgagttcatg 1621 cttgagcaag ccaataagtt tgctaaccat aaggccatct ggttccctta caacatggac 1681 tggcgcggtc gtgtttacgc tgtgtcaatg ttcaacccgc aaggtaacga tatgaccaaa 1741 ggactgctta cgctggcgaa aggtaaacca atcggtaagg aaggttacta ctggctgaaa 1801 atccacggtg caaactgtgc gggtgtcgat aaggttccgt tccctgagcg catcaagttc 1861 attgaggaaa accacgagaa catcatggct tgcgctaagt ctccactgga gaacacttgg 1921 tgggctgagc aagattctcc gttctgcttc cttgcgttct gctttgagta cgctggggta 1981 cagcaccacg gcctgagcta taactgctcc cttccgctgg cgtttgacgg gtcttgctct 2041 ggcatccagc acttctccgc gatgctccga gatgaggtag gtggtcgcgc ggttaacttg 2101 cttcctagtg aaaccgttca ggacatctac gggattgttg ctaagaaagt caacgagatt 2161 ctacaagcag acgcaatcaa tgggaccgat aacgaagtag ttaccgtgac cgatgagaac 2221 actggtgaaa tctctgagaa agtcaagctg ggcactaagg cactggctgg tcaatggctg 2281 gcttacggtg ttactcgcag tgtgactaag cgttcagtca tgacgctggc ttacgggtcc 2341 aaagagttcg gcttccgtca acaagtgctg gaagatacca ttcagccagc tattgattcc 2401 ggcaagggtc tgatgttcac tcagccgaat caggctgctg gatacatggc taagctgatt 2461 tgggaatctg tgagcgtgac ggtggtagct gcggttgaag caatgaactg gcttaagtct 2521 gctgctaagc tgctggctgc tgaggtcaaa gataagaaga ctggagagat tcttcgcaag 2581 cgttgcgctg tgcattgggt aactcctgat ggtttccctg tgtggcagga atacaagaag 2641 cctattcaga cgcgcttgaa cctgatgttc ctcggtcagt tccgcttaca gcctaccatt 2701 aacaccaaca aagatagcga gattgatgca cacaaacagg agtctggtat cgctcctaac 2761 tttgtacaca gccaagacgg tagccacctt cgtaagactg tagtgtgggc acacgagaag 2821 tacggaatcg aatcttttgc actgattcac gactccttcg gtaccattcc ggctgacgct 2881 gcgaacctgt tcaaagcagt gcgcgaaact atggttgaca catatgagtc ttgtgatgta 2941 ctggctgatt tctacgacca gttcgctgac cagttgcacg agtctcaatt ggacaaaatg 3001 ccagcacttc cggctaaagg taacttgaac ctccgtgaca tcttagagtc ggacttcgcg 3061 ttcgcataa //