BBa_K568004
type: ComponentDefinition
persistent identity: BBa K568004
display id: BBa_K568004
version: 1
was generated by: iGEM to SBOL conversion
description: Optogenetical AND-Gate blue light - without first promoter - choose your first input
created: 2011-09-13T11:00:00Z
modified: 2015-05-08T01:12:44Z
mutable provenance: tbd
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: tbd
discontinued: false
dominant: false
experience: experience/None
group u list: _736_
m user id: 0
owner id: 10022
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: tbd
star: false
creator: Team TU Munich 2011
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K228001
component: BBa_K238013
component: BBa_B0015
component: BBa_K228000
component: BBa_J44001
sequence annotation: BBa_K228001
sequence annotation: BBa_B0015
sequence annotation: BBa_K238013
sequence annotation: BBa_J44001
sequence annotation: BBa_K228000
sequence: https://synbiohub.org/public/igem/BBa_K568004_sequence/1

BBa_K228001
type: Component
persistent identity: component2128015
display id: component2128015
version: 1
top level: BBa_K568004
definition: BBa_K228001
access: public

BBa_K238013
type: Component
persistent identity: component2128029
display id: component2128029
version: 1
top level: BBa_K568004
definition: BLp
access: public

BBa_B0015
type: Component
persistent identity: component2128022
display id: component2128022
version: 1
top level: BBa_K568004
definition: BBa_B0015
access: public

BBa_K228000
type: Component
persistent identity: component2128033
display id: component2128033
version: 1
top level: BBa_K568004
definition: BBa_K228000
access: public

BBa_J44001
type: Component
persistent identity: component2128031
display id: component2128031
version: 1
top level: BBa_K568004
definition: BBa_J44001
access: public

BBa_K228001
type: SequenceAnnotation
persistent identity: annotation2128015
display id: annotation2128015
version: 1
top level: BBa_K568004
location: https://synbiohub.org/public/igem/BBa_K568004/annotation2128015/range2128015/1
component: BBa_K228001

https://synbiohub.org/public/igem/BBa_K568004/annotation2128015/range2128015/1
type: Range
persistent identity: range2128015
display id: range2128015
version: 1
top level: BBa_K568004
direction: direction/reverse
start: 1
end: 136
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2128022
display id: annotation2128022
version: 1
top level: BBa_K568004
location: https://synbiohub.org/public/igem/BBa_K568004/annotation2128022/range2128022/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K568004/annotation2128022/range2128022/1
type: Range
persistent identity: range2128022
display id: range2128022
version: 1
top level: BBa_K568004
direction: direction/reverse
start: 145
end: 273
orientation: inline

BBa_K238013
type: SequenceAnnotation
persistent identity: annotation2128029
display id: annotation2128029
version: 1
top level: BBa_K568004
location: https://synbiohub.org/public/igem/BBa_K568004/annotation2128029/range2128029/1
component: BBa_K238013

https://synbiohub.org/public/igem/BBa_K568004/annotation2128029/range2128029/1
type: Range
persistent identity: range2128029
display id: range2128029
version: 1
top level: BBa_K568004
direction: direction/reverse
start: 282
end: 367
orientation: inline

BBa_J44001
type: SequenceAnnotation
persistent identity: annotation2128031
display id: annotation2128031
version: 1
top level: BBa_K568004
location: https://synbiohub.org/public/igem/BBa_K568004/annotation2128031/range2128031/1
component: BBa_J44001

https://synbiohub.org/public/igem/BBa_K568004/annotation2128031/range2128031/1
type: Range
persistent identity: range2128031
display id: range2128031
version: 1
top level: BBa_K568004
direction: direction/reverse
start: 376
end: 390
orientation: inline

BBa_K228000
type: SequenceAnnotation
persistent identity: annotation2128033
display id: annotation2128033
version: 1
top level: BBa_K568004
location: https://synbiohub.org/public/igem/BBa_K568004/annotation2128033/range2128033/1
component: BBa_K228000

https://synbiohub.org/public/igem/BBa_K568004/annotation2128033/range2128033/1
type: Range
persistent identity: range2128033
display id: range2128033
version: 1
top level: BBa_K568004
direction: direction/reverse
start: 397
end: 3069
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_J44001
type: ComponentDefinition
persistent identity: BBa J44001
display id: BBa_J44001
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse RBS (RBSrev) -- corresponds to BBa_B0030
created: 2006-08-01T11:00:00Z
modified: 2015-08-31T04:08:48Z
mutable provenance: Cloned from synthetic oligos
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Reverse version of RBS BBa_B0030
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_71_
m user id: 0
owner id: 606
owning group id: 61
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Repeats in oligos caused unusual products during cloning
star: true
creator: Todd Eckdahl
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: Reverse RBS
sequence: https://synbiohub.org/public/igem/BBa_J44001_sequence/1

Reverse RBS
type: SequenceAnnotation
persistent identity: annotation1893199
display id: annotation1893199
version: 1
top level: BBa_J44001
location: https://synbiohub.org/public/igem/BBa_J44001/annotation1893199/range1893199/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_J44001/annotation1893199/range1893199/1
type: Range
persistent identity: range1893199
display id: range1893199
version: 1
top level: BBa_J44001
direction: direction/reverse
start: 1
end: 15
orientation: inline

BLp
type: ComponentDefinition
persistent identity: BBa K238013
display id: BBa_K238013
version: 1
was generated by: iGEM to SBOL conversion
description: Exact promoter of the blue light receptor
created: 2009-08-01T11:00:00Z
modified: 2015-05-08T01:11:36Z
mutable provenance: E.coli strain MC4100
owned by: james
owned by: myers
mutable description: ycgF/E regulated promoter region ycgf is a cytoplasmic receptor which responds to blue light. it contains a Blue light Using FAD-Domain (BLUF) and an EAL domain. when blue light strikes, the receptor changes conformation and dimerizes. This allows it to bind to ycgE repressor through its EAL-domain releasing the repressor from this promoter region. See reference for more info: Natalia Tschowri, Susan Busse and Regine Hengge, The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli. Genes and development23: 522-534 (2009)
discontinued: false
dominant: false
experience: experience/Works
group u list: _332_
m user id: 0
owner id: 4771
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: none
star: false
creator: Annelien Verfaillie
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: -35 box
sequence annotation: spacer
sequence annotation: -10 box
sequence annotation: inverted repeat part 1
sequence annotation: transcription start
sequence annotation: inverted repeat part 2
sequence: https://synbiohub.org/public/igem/BBa_K238013_sequence/1

-35 box
type: SequenceAnnotation
persistent identity: annotation2014609
display id: annotation2014609
version: 1
top level: BLp
location: https://synbiohub.org/public/igem/BBa_K238013/annotation2014609/range2014609/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K238013/annotation2014609/range2014609/1
type: Range
persistent identity: range2014609
display id: range2014609
version: 1
top level: BLp
direction: direction/forward
start: 28
end: 33
orientation: inline

spacer
type: SequenceAnnotation
persistent identity: annotation2014611
display id: annotation2014611
version: 1
top level: BLp
location: https://synbiohub.org/public/igem/BBa_K238013/annotation2014611/range2014611/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K238013/annotation2014611/range2014611/1
type: Range
persistent identity: range2014611
display id: range2014611
version: 1
top level: BLp
direction: direction/forward
start: 34
end: 51
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2014610
display id: annotation2014610
version: 1
top level: BLp
location: https://synbiohub.org/public/igem/BBa_K238013/annotation2014610/range2014610/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K238013/annotation2014610/range2014610/1
type: Range
persistent identity: range2014610
display id: range2014610
version: 1
top level: BLp
direction: direction/forward
start: 52
end: 58
orientation: inline

inverted repeat part 1
type: SequenceAnnotation
persistent identity: annotation2014612
display id: annotation2014612
version: 1
top level: BLp
location: https://synbiohub.org/public/igem/BBa_K238013/annotation2014612/range2014612/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K238013/annotation2014612/range2014612/1
type: Range
persistent identity: range2014612
display id: range2014612
version: 1
top level: BLp
direction: direction/forward
start: 53
end: 59
orientation: inline

transcription start
type: SequenceAnnotation
persistent identity: annotation2014614
display id: annotation2014614
version: 1
top level: BLp
location: https://synbiohub.org/public/igem/BBa_K238013/annotation2014614/range2014614/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K238013/annotation2014614/range2014614/1
type: Range
persistent identity: range2014614
display id: range2014614
version: 1
top level: BLp
direction: direction/forward
start: 64
end: 65
orientation: inline

inverted repeat part 2
type: SequenceAnnotation
persistent identity: annotation2014613
display id: annotation2014613
version: 1
top level: BLp
location: https://synbiohub.org/public/igem/BBa_K238013/annotation2014613/range2014613/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K238013/annotation2014613/range2014613/1
type: Range
persistent identity: range2014613
display id: range2014613
version: 1
top level: BLp
direction: direction/forward
start: 67
end: 73
orientation: inline

BBa_K228000
type: ComponentDefinition
persistent identity: BBa K228000
display id: BBa_K228000
version: 1
was generated by: iGEM to SBOL conversion
description: T7ptag(T7polymerase with amber mutation)
created: 2009-08-24T11:00:00Z
modified: 2015-05-08T01:11:32Z
mutable provenance: The T7ptag is standardlized from the plasmid kindly provided by Voigt Lab.
owned by: james
owned by: myers
mutable description: T7ptag is a coding sequence of T7 polymerase with 2 Amber mutations. The transcription of T7ptag gene can only lead to the generation of its mRNA, further translation into T7 RNA polymerase is blocked because of the amber mutation. Only in the presence of the Amber mutation suppressor BBa_K228001 SupD-tRNA, it can be translated into functional T7 polymerase. T7ptag is a component of the AND Gate constructed by the Voigt Lab(UCSF)
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4253
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
star: false
creator: Lin Min
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K228000_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2017714
display id: annotation2017714
version: 1
top level: BBa_K228000
location: https://synbiohub.org/public/igem/BBa_K228000/annotation2017714/range2017714/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K228000/annotation2017714/range2017714/1
type: Range
persistent identity: range2017714
display id: range2017714
version: 1
top level: BBa_K228000
direction: direction/forward
start: 1
end: 2673
orientation: inline

BBa_K228001
type: ComponentDefinition
persistent identity: BBa K228001
display id: BBa_K228001
version: 1
was generated by: iGEM to SBOL conversion
description: SupD-tRNA
created: 2009-08-24T11:00:00Z
modified: 2015-05-08T01:11:32Z
mutable provenance: SupD-tRNA again is from Voigt Lab(UCSF), We standardlize this part by PCR strategy.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The SupD-tRNA part functions as a tRNA, it suppresses the TAG amber stop codon and translate it into a Ser. There is no need to assembly SupD-tRNA with a rbs, since it is not translated. This part, together with the T7ptag part, make an AND Gate, that is, in the presence of both the 2 parts, T7 polymerase is tranlated into a functional polymerase that transcrips a downstream element constructed after a T7 promoter. Which is the output of the AND Gate.
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4253
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: There is a PstI site in the original sequence, but luckily it is near the 3' end of this part, so we designed a mutation in our primer and mutated the PstI site.
star: false
creator: Lin Min
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_K228001_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K568004_sequence/1
type: Sequence
persistent identity: BBa K568004 sequence
display id: BBa_K568004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caattcggagagatgccggagcggctgaacggaccggtctctaaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactacagatccttagcgaaagctaaggattttttttaagcttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagattgcaaaaaattaatttatcattctgtacacatatttcgtacaagtttgctattgttacttcacttaacattgattaacattttttactagagtttctcctctttaattactagatgaccatgattaccgtgcactagaataccattaacattgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgtaggaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagcta…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J44001_sequence/1
type: Sequence
persistent identity: BBa J44001 sequence
display id: BBa_J44001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttctcctctttaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K238013_sequence/1
type: Sequence
persistent identity: BBa K238013 sequence
display id: BBa_K238013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attgcaaaaaattaatttatcattctgtacacatatttcgtacaagtttgctattgttacttcacttaacattgattaacattttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228000_sequence/1
type: Sequence
persistent identity: BBa K228000 sequence
display id: BBa_K228000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccatgattaccgtgcactagaataccattaacattgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgtaggaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228001_sequence/1
type: Sequence
persistent identity: BBa K228001 sequence
display id: BBa_K228001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caattcggagagatgccggagcggctgaacggaccggtctctaaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactacagatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z