BBa_K568005
type: ComponentDefinition
persistent identity: BBa K568005
display id: BBa_K568005
version: 1
was generated by: iGEM to SBOL conversion
description: Intermediate optogenetical AND-Gate without T7ptag
created: 2011-09-13T11:00:00Z
modified: 2015-05-08T01:12:44Z
mutable provenance: tbd
owned by: james
owned by: myers
mutable description: Cloning intermediate. Last step of assembly.
discontinued: false
dominant: false
experience: experience/None
group u list: _736_
m user id: 0
owner id: 10022
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: tbd
star: false
creator: Team TU Munich 2011
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J44001
component: BBa_K228001
component: BBa_R0082
component: BBa_K238013
component: BBa_B0015
component: BBa_K322124
component: BBa_K322123
sequence annotation: BBa_K322123
sequence annotation: BBa_K322124
sequence annotation: BBa_R0082
sequence annotation: BBa_K228001
sequence annotation: BBa_B0015
sequence annotation: BBa_K238013
sequence annotation: BBa_J44001
sequence: https://synbiohub.org/public/igem/BBa_K568005_sequence/1

BBa_J44001
type: Component
persistent identity: component2128107
display id: component2128107
version: 1
top level: BBa_K568005
definition: BBa_J44001
access: public

BBa_K228001
type: Component
persistent identity: component2128091
display id: component2128091
version: 1
top level: BBa_K568005
definition: BBa_K228001
access: public

BBa_R0082
type: Component
persistent identity: component2128090
display id: component2128090
version: 1
top level: BBa_K568005
definition: OmpR
access: public

BBa_K238013
type: Component
persistent identity: component2128105
display id: component2128105
version: 1
top level: BBa_K568005
definition: BLp
access: public

BBa_B0015
type: Component
persistent identity: component2128098
display id: component2128098
version: 1
top level: BBa_K568005
definition: BBa_B0015
access: public

BBa_K322124
type: Component
persistent identity: component2128084
display id: component2128084
version: 1
top level: BBa_K568005
definition: BBa_K322124
access: public

BBa_K322123
type: Component
persistent identity: component2128081
display id: component2128081
version: 1
top level: BBa_K568005
definition: BBa_K322123
access: public

BBa_K322123
type: SequenceAnnotation
persistent identity: annotation2128081
display id: annotation2128081
version: 1
top level: BBa_K568005
location: https://synbiohub.org/public/igem/BBa_K568005/annotation2128081/range2128081/1
component: BBa_K322123

https://synbiohub.org/public/igem/BBa_K568005/annotation2128081/range2128081/1
type: Range
persistent identity: range2128081
display id: range2128081
version: 1
top level: BBa_K568005
direction: direction/reverse
start: 1
end: 1399
orientation: inline

BBa_K322124
type: SequenceAnnotation
persistent identity: annotation2128084
display id: annotation2128084
version: 1
top level: BBa_K568005
location: https://synbiohub.org/public/igem/BBa_K568005/annotation2128084/range2128084/1
component: BBa_K322124

https://synbiohub.org/public/igem/BBa_K568005/annotation2128084/range2128084/1
type: Range
persistent identity: range2128084
display id: range2128084
version: 1
top level: BBa_K568005
direction: direction/reverse
start: 1408
end: 3660
orientation: inline

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2128090
display id: annotation2128090
version: 1
top level: BBa_K568005
location: https://synbiohub.org/public/igem/BBa_K568005/annotation2128090/range2128090/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_K568005/annotation2128090/range2128090/1
type: Range
persistent identity: range2128090
display id: range2128090
version: 1
top level: BBa_K568005
direction: direction/reverse
start: 3669
end: 3776
orientation: inline

BBa_K228001
type: SequenceAnnotation
persistent identity: annotation2128091
display id: annotation2128091
version: 1
top level: BBa_K568005
location: https://synbiohub.org/public/igem/BBa_K568005/annotation2128091/range2128091/1
component: BBa_K228001

https://synbiohub.org/public/igem/BBa_K568005/annotation2128091/range2128091/1
type: Range
persistent identity: range2128091
display id: range2128091
version: 1
top level: BBa_K568005
direction: direction/reverse
start: 3785
end: 3920
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2128098
display id: annotation2128098
version: 1
top level: BBa_K568005
location: https://synbiohub.org/public/igem/BBa_K568005/annotation2128098/range2128098/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K568005/annotation2128098/range2128098/1
type: Range
persistent identity: range2128098
display id: range2128098
version: 1
top level: BBa_K568005
direction: direction/reverse
start: 3929
end: 4057
orientation: inline

BBa_K238013
type: SequenceAnnotation
persistent identity: annotation2128105
display id: annotation2128105
version: 1
top level: BBa_K568005
location: https://synbiohub.org/public/igem/BBa_K568005/annotation2128105/range2128105/1
component: BBa_K238013

https://synbiohub.org/public/igem/BBa_K568005/annotation2128105/range2128105/1
type: Range
persistent identity: range2128105
display id: range2128105
version: 1
top level: BBa_K568005
direction: direction/reverse
start: 4066
end: 4151
orientation: inline

BBa_J44001
type: SequenceAnnotation
persistent identity: annotation2128107
display id: annotation2128107
version: 1
top level: BBa_K568005
location: https://synbiohub.org/public/igem/BBa_K568005/annotation2128107/range2128107/1
component: BBa_J44001

https://synbiohub.org/public/igem/BBa_K568005/annotation2128107/range2128107/1
type: Range
persistent identity: range2128107
display id: range2128107
version: 1
top level: BBa_K568005
direction: direction/reverse
start: 4160
end: 4174
orientation: inline

OmpR
type: ComponentDefinition
persistent identity: BBa R0082
display id: BBa_R0082
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (OmpR, positive)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: NC_000193 E. coli K12
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1. An alternate version, BBa_R0083, cuts out the C2 and C3 sites. The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown.
star: true
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: C1 OmpR
sequence annotation: C2 OmpR
sequence annotation: C3 OmpR
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0082_sequence/1

C1 OmpR
type: SequenceAnnotation
persistent identity: annotation301154
display id: annotation301154
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
type: Range
persistent identity: range301154
display id: range301154
version: 1
top level: OmpR
direction: direction/forward
start: 13
end: 30
orientation: inline

C2 OmpR
type: SequenceAnnotation
persistent identity: annotation301155
display id: annotation301155
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
type: Range
persistent identity: range301155
display id: range301155
version: 1
top level: OmpR
direction: direction/forward
start: 34
end: 51
orientation: inline

C3 OmpR
type: SequenceAnnotation
persistent identity: annotation301156
display id: annotation301156
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
type: Range
persistent identity: range301156
display id: range301156
version: 1
top level: OmpR
direction: direction/forward
start: 54
end: 71
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301166
display id: annotation301166
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
type: Range
persistent identity: range301166
display id: range301166
version: 1
top level: OmpR
direction: direction/forward
start: 75
end: 80
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301167
display id: annotation301167
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
type: Range
persistent identity: range301167
display id: range301167
version: 1
top level: OmpR
direction: direction/forward
start: 98
end: 103
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K322123
type: ComponentDefinition
persistent identity: BBa K322123
display id: BBa_K322123
version: 1
was generated by: iGEM to SBOL conversion
description: Phycobilin synthesis operon without terminator
created: 2010-09-24T11:00:00Z
modified: 2015-05-08T01:11:59Z
mutable provenance: This is derived from K098010 but PCR was used to remove the terminator, so that cph8 could be added at the end of the operon.
owned by: james
owned by: myers
mutable description: Phycobilin is chromophores (light-capturing molecules) found in cyanobacteria and in the chloroplasts of red algae, glaucophytes and some cryptomonads (though not in green algae and higher plants)， used in light sensor.
discontinued: false
dominant: false
experience: experience/None
group u list: _441_
m user id: 0
owner id: 6225
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: The actual forward sequence has the same changes at the start as seen in clone H11 described above. The reverse sequence is as expected. Features same as for the one above apart from the terminator.
star: false
creator: Chris French and Richard Edward Partridge-Hicks
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: J23110
sequence annotation: B0032
sequence annotation: Heme oxygenase
sequence annotation: rbs
sequence annotation: pcyA
sequence: https://synbiohub.org/public/igem/BBa_K322123_sequence/1

J23110
type: SequenceAnnotation
persistent identity: annotation2083043
display id: annotation2083043
version: 1
top level: BBa_K322123
location: https://synbiohub.org/public/igem/BBa_K322123/annotation2083043/range2083043/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K322123/annotation2083043/range2083043/1
type: Range
persistent identity: range2083043
display id: range2083043
version: 1
top level: BBa_K322123
direction: direction/forward
start: 1
end: 35
orientation: inline

B0032
type: SequenceAnnotation
persistent identity: annotation2083044
display id: annotation2083044
version: 1
top level: BBa_K322123
location: https://synbiohub.org/public/igem/BBa_K322123/annotation2083044/range2083044/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K322123/annotation2083044/range2083044/1
type: Range
persistent identity: range2083044
display id: range2083044
version: 1
top level: BBa_K322123
direction: direction/forward
start: 43
end: 54
orientation: inline

Heme oxygenase
type: SequenceAnnotation
persistent identity: annotation2083045
display id: annotation2083045
version: 1
top level: BBa_K322123
location: https://synbiohub.org/public/igem/BBa_K322123/annotation2083045/range2083045/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K322123/annotation2083045/range2083045/1
type: Range
persistent identity: range2083045
display id: range2083045
version: 1
top level: BBa_K322123
direction: direction/forward
start: 63
end: 629
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2083046
display id: annotation2083046
version: 1
top level: BBa_K322123
location: https://synbiohub.org/public/igem/BBa_K322123/annotation2083046/range2083046/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K322123/annotation2083046/range2083046/1
type: Range
persistent identity: range2083046
display id: range2083046
version: 1
top level: BBa_K322123
direction: direction/forward
start: 630
end: 644
orientation: inline

pcyA
type: SequenceAnnotation
persistent identity: annotation2083047
display id: annotation2083047
version: 1
top level: BBa_K322123
location: https://synbiohub.org/public/igem/BBa_K322123/annotation2083047/range2083047/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K322123/annotation2083047/range2083047/1
type: Range
persistent identity: range2083047
display id: range2083047
version: 1
top level: BBa_K322123
direction: direction/forward
start: 650
end: 1396
orientation: inline

BBa_K322124
type: ComponentDefinition
persistent identity: BBa K322124
display id: BBa_K322124
version: 1
was generated by: iGEM to SBOL conversion
description: Red light sensor Cph8 (from Coliroid) with RBS
created: 2010-09-24T11:00:00Z
modified: 2015-05-08T01:11:59Z
mutable provenance: BBa_I15010 (Photosensing Chimaera (Cph8) (not working, but amplified out of the whole construct))
owned by: james
owned by: myers
mutable description: Cph8 is a combination of cph1 and envZ which, together with PCB, form a transmembrane, histidine kinase-based light sensing system. It contains the photoreceptor from Cph1 and the histidine kinase and response-regulator from EnvZ???OmpR, producing a particularly strongresponse to light. Features: cph8 coding sequence 16 to 2250, RBS 6 to 10.
discontinued: false
dominant: false
experience: experience/None
group u list: _441_
m user id: 0
owner id: 6225
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Notes: we were unable to revive BioBrick I15010 with the coding sequence of the cph8 red light sensor fusion protein, so we sought to amplify it from BioBrick BBa_M30109. This led to numerous problems. It is clear from the Registry sequence data that M30109 is not what it is supposed to be. It does include cph8 with a ribosome binding site, but the RBS is not joined to cph8 by a standard scar; promoter R0040 may be present, but there appears to be an EcoRI site a little upstream of the ribosome binding site so that, when we cloned our PCR product, we failed to recover the promoter region; also the PstI site in cph8, which was mutated out in I15010, is still present in the version in M30109. We therefore performed corrective PCR to obtain a clone with the correct prefix, a ribosome binding site, and the cph8 coding sequence with the PstI site properly removed, which we present here, cloned in pSB1C3.
star: false
creator: Chris French and Richard Edward Partridge-Hicks
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: RBS
sequence annotation: Cph8
sequence: https://synbiohub.org/public/igem/BBa_K322124_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2083481
display id: annotation2083481
version: 1
top level: BBa_K322124
location: https://synbiohub.org/public/igem/BBa_K322124/annotation2083481/range2083481/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K322124/annotation2083481/range2083481/1
type: Range
persistent identity: range2083481
display id: range2083481
version: 1
top level: BBa_K322124
direction: direction/forward
start: 6
end: 10
orientation: inline

Cph8
type: SequenceAnnotation
persistent identity: annotation2083482
display id: annotation2083482
version: 1
top level: BBa_K322124
location: https://synbiohub.org/public/igem/BBa_K322124/annotation2083482/range2083482/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K322124/annotation2083482/range2083482/1
type: Range
persistent identity: range2083482
display id: range2083482
version: 1
top level: BBa_K322124
direction: direction/forward
start: 16
end: 2250
orientation: inline

BBa_J44001
type: ComponentDefinition
persistent identity: BBa J44001
display id: BBa_J44001
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse RBS (RBSrev) -- corresponds to BBa_B0030
created: 2006-08-01T11:00:00Z
modified: 2015-08-31T04:08:48Z
mutable provenance: Cloned from synthetic oligos
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Reverse version of RBS BBa_B0030
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_71_
m user id: 0
owner id: 606
owning group id: 61
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Repeats in oligos caused unusual products during cloning
star: true
creator: Todd Eckdahl
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: Reverse RBS
sequence: https://synbiohub.org/public/igem/BBa_J44001_sequence/1

Reverse RBS
type: SequenceAnnotation
persistent identity: annotation1893199
display id: annotation1893199
version: 1
top level: BBa_J44001
location: https://synbiohub.org/public/igem/BBa_J44001/annotation1893199/range1893199/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_J44001/annotation1893199/range1893199/1
type: Range
persistent identity: range1893199
display id: range1893199
version: 1
top level: BBa_J44001
direction: direction/reverse
start: 1
end: 15
orientation: inline

BLp
type: ComponentDefinition
persistent identity: BBa K238013
display id: BBa_K238013
version: 1
was generated by: iGEM to SBOL conversion
description: Exact promoter of the blue light receptor
created: 2009-08-01T11:00:00Z
modified: 2015-05-08T01:11:36Z
mutable provenance: E.coli strain MC4100
owned by: james
owned by: myers
mutable description: ycgF/E regulated promoter region ycgf is a cytoplasmic receptor which responds to blue light. it contains a Blue light Using FAD-Domain (BLUF) and an EAL domain. when blue light strikes, the receptor changes conformation and dimerizes. This allows it to bind to ycgE repressor through its EAL-domain releasing the repressor from this promoter region. See reference for more info: Natalia Tschowri, Susan Busse and Regine Hengge, The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli. Genes and development23: 522-534 (2009)
discontinued: false
dominant: false
experience: experience/Works
group u list: _332_
m user id: 0
owner id: 4771
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: none
star: false
creator: Annelien Verfaillie
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: -35 box
sequence annotation: spacer
sequence annotation: -10 box
sequence annotation: inverted repeat part 1
sequence annotation: transcription start
sequence annotation: inverted repeat part 2
sequence: https://synbiohub.org/public/igem/BBa_K238013_sequence/1

-35 box
type: SequenceAnnotation
persistent identity: annotation2014609
display id: annotation2014609
version: 1
top level: BLp
location: https://synbiohub.org/public/igem/BBa_K238013/annotation2014609/range2014609/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K238013/annotation2014609/range2014609/1
type: Range
persistent identity: range2014609
display id: range2014609
version: 1
top level: BLp
direction: direction/forward
start: 28
end: 33
orientation: inline

spacer
type: SequenceAnnotation
persistent identity: annotation2014611
display id: annotation2014611
version: 1
top level: BLp
location: https://synbiohub.org/public/igem/BBa_K238013/annotation2014611/range2014611/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K238013/annotation2014611/range2014611/1
type: Range
persistent identity: range2014611
display id: range2014611
version: 1
top level: BLp
direction: direction/forward
start: 34
end: 51
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2014610
display id: annotation2014610
version: 1
top level: BLp
location: https://synbiohub.org/public/igem/BBa_K238013/annotation2014610/range2014610/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K238013/annotation2014610/range2014610/1
type: Range
persistent identity: range2014610
display id: range2014610
version: 1
top level: BLp
direction: direction/forward
start: 52
end: 58
orientation: inline

inverted repeat part 1
type: SequenceAnnotation
persistent identity: annotation2014612
display id: annotation2014612
version: 1
top level: BLp
location: https://synbiohub.org/public/igem/BBa_K238013/annotation2014612/range2014612/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K238013/annotation2014612/range2014612/1
type: Range
persistent identity: range2014612
display id: range2014612
version: 1
top level: BLp
direction: direction/forward
start: 53
end: 59
orientation: inline

transcription start
type: SequenceAnnotation
persistent identity: annotation2014614
display id: annotation2014614
version: 1
top level: BLp
location: https://synbiohub.org/public/igem/BBa_K238013/annotation2014614/range2014614/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K238013/annotation2014614/range2014614/1
type: Range
persistent identity: range2014614
display id: range2014614
version: 1
top level: BLp
direction: direction/forward
start: 64
end: 65
orientation: inline

inverted repeat part 2
type: SequenceAnnotation
persistent identity: annotation2014613
display id: annotation2014613
version: 1
top level: BLp
location: https://synbiohub.org/public/igem/BBa_K238013/annotation2014613/range2014613/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K238013/annotation2014613/range2014613/1
type: Range
persistent identity: range2014613
display id: range2014613
version: 1
top level: BLp
direction: direction/forward
start: 67
end: 73
orientation: inline

BBa_K228001
type: ComponentDefinition
persistent identity: BBa K228001
display id: BBa_K228001
version: 1
was generated by: iGEM to SBOL conversion
description: SupD-tRNA
created: 2009-08-24T11:00:00Z
modified: 2015-05-08T01:11:32Z
mutable provenance: SupD-tRNA again is from Voigt Lab(UCSF), We standardlize this part by PCR strategy.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The SupD-tRNA part functions as a tRNA, it suppresses the TAG amber stop codon and translate it into a Ser. There is no need to assembly SupD-tRNA with a rbs, since it is not translated. This part, together with the T7ptag part, make an AND Gate, that is, in the presence of both the 2 parts, T7 polymerase is tranlated into a functional polymerase that transcrips a downstream element constructed after a T7 promoter. Which is the output of the AND Gate.
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4253
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: There is a PstI site in the original sequence, but luckily it is near the 3' end of this part, so we designed a mutation in our primer and mutated the PstI site.
star: false
creator: Lin Min
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_K228001_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K568005_sequence/1
type: Sequence
persistent identity: BBa K568005 sequence
display id: BBa_K568005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtattatgctagctactagagtcacacaggaaaggtgcacatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctagttaaagaggagaaaggatccatggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataatactagagaggagaaaggtacccatggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccgcccacctgattcagccccacggtttagtggtggtcctgcaagaaccagacctcaccatcagccaaattagcgccaactgcaccggcattttagggcgatcgccagaggatttgttgggcagaaccctaggggaagtgtttgatagctttcagattgatcccatccagagtcgcctaacggccggacaaatcagcagcctcaaccccagtaaactttgggcgcgggtcatgggggacgactttgtcatttttgacggggtttttcatcgcaacagtgacggtttattggtatgtgaactcgagccagcctacacttccgataatctgcccttcctcggtttttatcacatggccaacgctgccctgaatcggttgcgccaacaagctaatctacgggatttctacgatgttattgtcgaagaagtccgccgtatgactggctttgaccgggtgatgctataccgctttgatgaaaataaccacggtgatgtcattgccgaagataaacggga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0082_sequence/1
type: Sequence
persistent identity: BBa R0082 sequence
display id: BBa_R0082_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K322123_sequence/1
type: Sequence
persistent identity: BBa K322123 sequence
display id: BBa_K322123_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtattatgctagctactagagtcacacaggaaaggtgcacatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctagttaaagaggagaaaggatccatggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K322124_sequence/1
type: Sequence
persistent identity: BBa K322124 sequence
display id: BBa_K322124_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aggagaaaggtacccatggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccgcccacctgattcagccccacggtttagtggtggtcctgcaagaaccagacctcaccatcagccaaattagcgccaactgcaccggcattttagggcgatcgccagaggatttgttgggcagaaccctaggggaagtgtttgatagctttcagattgatcccatccagagtcgcctaacggccggacaaatcagcagcctcaaccccagtaaactttgggcgcgggtcatgggggacgactttgtcatttttgacggggtttttcatcgcaacagtgacggtttattggtatgtgaactcgagccagcctacacttccgataatctgcccttcctcggtttttatcacatggccaacgctgccctgaatcggttgcgccaacaagctaatctacgggatttctacgatgttattgtcgaagaagtccgccgtatgactggctttgaccgggtgatgctataccgctttgatgaaaataaccacggtgatgtcattgccgaagataaacgggatgatatggaaccctatttgggcctgcactatcccgaatcggatattccccaacccgcccgtcggctatttatccacaaccccattcgagtaattcccgatgtttatggtgtggcggtgcccctgaccccagcggttaaccccagcaccaaccgagcggtggatttaacagaatccattctgcgcagtgcgtaccattgccacttgacctatctgaaaaatatgggggtaggagcgtctttaaccatttccctaattaaggacggccatctctgggggctcattgcctgccaccatcaaacccccaaagtaattccctttgaactgcgtaaagcctgcgaattttttggtcgggtggtgtttagcaacatttccgcccaggaagatacggaaaccttcgattaccgggtgcagctggcggagcatgaagcggttttattggacaaaatgaccacggcggcggattttgtcgaaggattaactaatcatcccgatcgcctgttgggattaacgggctcccagggggcggccatttgctttggggaaaaattgattttagtaggggaaaccccggacgagaaagcagtgcaatatttactgcaatggttggagaatcgggaagtgcaagacgttttcttcacctcttccctctcacaaatttatcctgatgcagtgaattttaaatccgtggccagtggcttattggccattcccattgcccgtcacaactttttgctctggtttcgccctgaagtgttgcaaacggttaattggggcggtgacccaaatcatgcttacgaagctacccaggaagacggtaaaatcgagctccatccccgccaatcctttgacctctggaaagaaattgtccgactccaatctttgccctggcaatcggtggaaatccaaagtgccctggccctgaaaaaggcgatcgtcaacctcattttgcgccaggcagaagaattgcatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctgggtcaaagtcagcagcggaacggagccgaatcgcgcctg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J44001_sequence/1
type: Sequence
persistent identity: BBa J44001 sequence
display id: BBa_J44001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttctcctctttaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K238013_sequence/1
type: Sequence
persistent identity: BBa K238013 sequence
display id: BBa_K238013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attgcaaaaaattaatttatcattctgtacacatatttcgtacaagtttgctattgttacttcacttaacattgattaacattttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228001_sequence/1
type: Sequence
persistent identity: BBa K228001 sequence
display id: BBa_K228001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caattcggagagatgccggagcggctgaacggaccggtctctaaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactacagatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z