LOCUS BBa_K568008 4572 bp DNA linear 22-Dec-2024 DEFINITION Red light sensor with GFP reporter ACCESSION BBa_K568008 VERSION BBa_K568008.1 FEATURES Location/Qualifiers promoter 1..35 /label=J23110 RBS 43..54 /label=B0032 CDS 63..629 /label=Heme oxygenase RBS 630..644 /label=rbs CDS 650..1396 /label=pcyA misc_feature 1..1399 /label=BBa_K322123 RBS 1413..1417 /label=RBS CDS 1423..3657 /label=Cph8 misc_feature 1408..3660 /label=BBa_K322124 misc_feature 3681..3698 /label=C1 OmpR misc_feature 3702..3719 /label=C2 OmpR misc_feature 3722..3739 /label=C3 OmpR promoter 3743..3748 /label=-35 promoter 3766..3771 /label=-10 promoter 3669..3776 /label=BBa_R0082 misc_feature 1..3776 /label=BBa_K568000 RBS 3785..3799 /label=RBS-1\Strong RBS 3792..3796 /label=RBS misc_feature 3785..3799 /label=BBa_B0030 RBS 3785..3799 /label=BBa_B0030 CDS 3806..4525 /label=GFP protein CDS 3806..4525 /label=BBa_E0040 misc_feature 4534..4572 /label=B1006 stem_loop 4543..4564 /label=modified thr terminator polyA_site 4565..4572 /label=PolyA polyA_site 4534..4542 /label=PolyA terminator 4534..4572 /label=BBa_B1006 misc_feature 3785..4572 /label=BBa_K081012 ORIGIN 1 tttacggcta gctcagtcct aggtattatg ctagctacta gagtcacaca ggaaaggtgc 61 acatggaaga ggaaatggca aaatttaagg accatcccat cctcagccac atttacttcc 121 ccgaactcaa ccgcaaacaa agcctagagc aagacctgca attctattac ggctccaact 181 ggcggcaaga agtgaaaatt tctgccgctg gccaagccta tgtggaccga gtccggcaag 241 tggccgctac ggcccctgaa ttgttggtgg cccattccta cacccgttac ctgggggatc 301 tttccggcgg tcaaattctc aagaaaattg cccaaaatgc catgaatctc cacgatggtg 361 gcacagcttt ctatgaattt gccgacattg atgacgaaaa ggcttttaaa aatacctacc 421 gtcaagctat gaatgatctg cccattgacc aagccaccgc cgaacggatt gtggatgaag 481 ccaatgacgc ctttgccatg aacatgaaaa tgttcaacga acttgaaggc aacctgatca 541 aggcgatcgg cattatggtg ttcaacagcc tcacccgtcg ccgcagtcaa ggcagcaccg 601 aagttggcct cgccacctcc gaaggctagt taaagaggag aaaggatcca tggccgtcac 661 tgatttaagt ttgaccaatt cttccctgat gcctacgttg aacccgatga ttcaacagtt 721 ggccctggcg atcgccgcta gttggcaaag tttacccctc aagccctatc aattgccgga 781 ggatttgggc tacgtagaag gccgcctgga aggggaaaag ttagtgattg aaaatcggtg 841 ctaccaaacg ccccagtttc gcaaaatgca tttggagttg gccaaggtgg gcaaagggtt 901 ggatattctc cactgtgtaa tgtttcctga gcctttatac ggtctacctt tgtttggctg 961 tgacattgtg gccggccccg gtggagtaag tgcggctatt gcggatctat cccccaccca 1021 aagcgatcgc caattgcccg cagcgtacca aaaatcattg gcagagctag gccagccaga 1081 atttgagcaa caacgggaat tgcccccctg gggagaaata ttttctgaat attgtttatt 1141 catccgtccc agcaatgtca ctgaagaaga aagatttgta caaagggtag tggacttttt 1201 gcaaattcat tgtcaccaat ccatcgttgc cgaacccttg tctgaagctc aaactttgga 1261 gcaccgtcag gggcaaattc attactgcca acaacaacag aaaaatgata aaacccgtcg 1321 ggtactggaa aaagcttttg gggaagcttg ggcggaacgg tatatgagcc aagtcttatt 1381 tgatgttatc caataataat actagagagg agaaaggtac ccatggccac caccgtacaa 1441 ctcagcgacc aatccctccg tcagctagaa accctcgcca tccacaccgc ccacctgatt 1501 cagccccacg gtttagtggt ggtcctgcaa gaaccagacc tcaccatcag ccaaattagc 1561 gccaactgca ccggcatttt agggcgatcg ccagaggatt tgttgggcag aaccctaggg 1621 gaagtgtttg atagctttca gattgatccc atccagagtc gcctaacggc cggacaaatc 1681 agcagcctca accccagtaa actttgggcg cgggtcatgg gggacgactt tgtcattttt 1741 gacggggttt ttcatcgcaa cagtgacggt ttattggtat gtgaactcga gccagcctac 1801 acttccgata atctgccctt cctcggtttt tatcacatgg ccaacgctgc cctgaatcgg 1861 ttgcgccaac aagctaatct acgggatttc tacgatgtta ttgtcgaaga agtccgccgt 1921 atgactggct ttgaccgggt gatgctatac cgctttgatg aaaataacca cggtgatgtc 1981 attgccgaag ataaacggga tgatatggaa ccctatttgg gcctgcacta tcccgaatcg 2041 gatattcccc aacccgcccg tcggctattt atccacaacc ccattcgagt aattcccgat 2101 gtttatggtg tggcggtgcc cctgacccca gcggttaacc ccagcaccaa ccgagcggtg 2161 gatttaacag aatccattct gcgcagtgcg taccattgcc acttgaccta tctgaaaaat 2221 atgggggtag gagcgtcttt aaccatttcc ctaattaagg acggccatct ctgggggctc 2281 attgcctgcc accatcaaac ccccaaagta attccctttg aactgcgtaa agcctgcgaa 2341 ttttttggtc gggtggtgtt tagcaacatt tccgcccagg aagatacgga aaccttcgat 2401 taccgggtgc agctggcgga gcatgaagcg gttttattgg acaaaatgac cacggcggcg 2461 gattttgtcg aaggattaac taatcatccc gatcgcctgt tgggattaac gggctcccag 2521 ggggcggcca tttgctttgg ggaaaaattg attttagtag gggaaacccc ggacgagaaa 2581 gcagtgcaat atttactgca atggttggag aatcgggaag tgcaagacgt tttcttcacc 2641 tcttccctct cacaaattta tcctgatgca gtgaatttta aatccgtggc cagtggctta 2701 ttggccattc ccattgcccg tcacaacttt ttgctctggt ttcgccctga agtgttgcaa 2761 acggttaatt ggggcggtga cccaaatcat gcttacgaag ctacccagga agacggtaaa 2821 atcgagctcc atccccgcca atcctttgac ctctggaaag aaattgtccg actccaatct 2881 ttgccctggc aatcggtgga aatccaaagt gccctggccc tgaaaaaggc gatcgtcaac 2941 ctcattttgc gccaggcaga agaattgcat atggcggctg gtgttaagca actggcggat 3001 gaccgcacgc tgctgatggc gggggtaagt cacgacttgc gcacgccgct gacgcgtatt 3061 cgcctggcga ctgagatgat gagcgagcag gatggctatc tggcagaatc gatcaataaa 3121 gatatcgaag agtgcaacgc catcattgag cagtttatcg actacctgcg caccgggcag 3181 gagatgccga tggaaatggc ggatcttaat gcagtactcg gtgaggtgat tgctgccgaa 3241 agtggctatg agcgggaaat tgaaaccgcg ctttaccccg gcagcattga agtgaaaatg 3301 cacccgctgt cgatcaaacg cgcggtggcg aatatggtgg tcaacgccgc ccgttatggc 3361 aatggctggg tcaaagtcag cagcggaacg gagccgaatc gcgcctggtt ccaggtggaa 3421 gatgacggtc cgggaattgc gccggaacaa cgtaagcacc tgttccagcc gtttgtccgc 3481 ggcgacagtg cgcgcaccat tagcggcacg ggattagggc tggcaattgt gcagcgtatc 3541 gtggataacc ataacgggat gctggagctt ggcaccagcg agcggggcgg gctttccatt 3601 cgcgcctggc tgccagtgcc ggtaacgcgg gcgcagggca tgacaaaaga agggtaataa 3661 tactagagtc ccttgcattt acattttgaa acatctatag cgataaatga aacatcttaa 3721 aagttttagt atcatattcg tgttggatta ttctgcattt ttggggagaa tggacttact 3781 agagattaaa gaggagaaat actagatgcg taaaggagaa gaacttttca ctggagttgt 3841 cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga 3901 gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa 3961 actacctgtt ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc 4021 gagataccca gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta 4081 tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt 4141 caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga 4201 agatggaaac attcttggac acaaattgga atacaactat aactcacaca atgtatacat 4261 catggcagac aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga 4321 agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc 4381 tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa 4441 cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg 4501 catggatgaa ctatacaaat aataatacta gagaaaaaaa aaccccgccc ctgacagggc 4561 ggggtttttt tt //