BBa_K568008
type: ComponentDefinition
persistent identity: BBa K568008
display id: BBa_K568008
version: 1
was generated by: iGEM to SBOL conversion
description: Red light sensor with GFP reporter
created: 2011-10-25T11:00:00Z
modified: 2015-05-08T01:12:44Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: Red light sensor with GFP reporter
discontinued: false
dominant: false
experience: experience/Fails
group u list: _736_
m user id: 0
owner id: 10022
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: Team TU Munich 2011
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K081012
component: BBa_K568000
sequence annotation: BBa_K568000
sequence annotation: BBa_K081012
sequence: https://synbiohub.org/public/igem/BBa_K568008_sequence/1

BBa_K081012
type: Component
persistent identity: component2160633
display id: component2160633
version: 1
top level: BBa_K568008
definition: BBa_K081012
access: public

BBa_K568000
type: Component
persistent identity: component2160622
display id: component2160622
version: 1
top level: BBa_K568008
definition: BBa_K568000
access: public

BBa_K568000
type: SequenceAnnotation
persistent identity: annotation2160622
display id: annotation2160622
version: 1
top level: BBa_K568008
location: https://synbiohub.org/public/igem/BBa_K568008/annotation2160622/range2160622/1
component: BBa_K568000

https://synbiohub.org/public/igem/BBa_K568008/annotation2160622/range2160622/1
type: Range
persistent identity: range2160622
display id: range2160622
version: 1
top level: BBa_K568008
direction: direction/reverse
start: 1
end: 3776
orientation: inline

BBa_K081012
type: SequenceAnnotation
persistent identity: annotation2160633
display id: annotation2160633
version: 1
top level: BBa_K568008
location: https://synbiohub.org/public/igem/BBa_K568008/annotation2160633/range2160633/1
component: BBa_K081012

https://synbiohub.org/public/igem/BBa_K568008/annotation2160633/range2160633/1
type: Range
persistent identity: range2160633
display id: range2160633
version: 1
top level: BBa_K568008
direction: direction/reverse
start: 3785
end: 4572
orientation: inline

BBa_K081012
type: ComponentDefinition
persistent identity: BBa K081012
display id: BBa_K081012
version: 1
was generated by: iGEM to SBOL conversion
description: GFP protein generator - PoPS->GFP
created: 2008-10-17T11:00:00Z
modified: 2015-05-08T01:08:35Z
mutable provenance: RBS: GFP: T:
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PoPS in -> GFP out Strong RBS (efficiency=0.6).
discontinued: false
dominant: false
experience: experience/Works
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: We used BioBrick Standard Assembly.
star: true
creator: Lorenzo Pasotti, Paolo Magni
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_E0040
component: BBa_B0030
component: BBa_B1006
sequence annotation: BBa_B0030
sequence annotation: BBa_E0040
sequence annotation: BBa_B1006
sequence: https://synbiohub.org/public/igem/BBa_K081012_sequence/1

BBa_E0040
type: Component
persistent identity: component1981957
display id: component1981957
version: 1
top level: BBa_K081012
definition: GFP
access: public

BBa_B0030
type: Component
persistent identity: component1981954
display id: component1981954
version: 1
top level: BBa_K081012
definition: BBa_B0030
access: public

BBa_B1006
type: Component
persistent identity: component1981962
display id: component1981962
version: 1
top level: BBa_K081012
definition: BBa_B1006
access: public

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1981954
display id: annotation1981954
version: 1
top level: BBa_K081012
location: https://synbiohub.org/public/igem/BBa_K081012/annotation1981954/range1981954/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081012/annotation1981954/range1981954/1
type: Range
persistent identity: range1981954
display id: range1981954
version: 1
top level: BBa_K081012
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1981957
display id: annotation1981957
version: 1
top level: BBa_K081012
location: https://synbiohub.org/public/igem/BBa_K081012/annotation1981957/range1981957/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K081012/annotation1981957/range1981957/1
type: Range
persistent identity: range1981957
display id: range1981957
version: 1
top level: BBa_K081012
direction: direction/reverse
start: 22
end: 741
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation1981962
display id: annotation1981962
version: 1
top level: BBa_K081012
location: https://synbiohub.org/public/igem/BBa_K081012/annotation1981962/range1981962/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K081012/annotation1981962/range1981962/1
type: Range
persistent identity: range1981962
display id: range1981962
version: 1
top level: BBa_K081012
direction: direction/reverse
start: 750
end: 788
orientation: inline

BBa_K568000
type: ComponentDefinition
persistent identity: BBa K568000
display id: BBa_K568000
version: 1
was generated by: iGEM to SBOL conversion
description: Red light sensor
created: 2011-08-31T11:00:00Z
modified: 2015-05-08T01:12:43Z
mutable provenance: PCR on relevant part of K322127
owned by: james
owned by: myers
mutable description: OmpR Promotor will be activated upon illumination with red light
discontinued: false
dominant: false
experience: experience/Fails
group u list: _736_
m user id: 0
owner id: 9896
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: PCR product on relevant part of K322127
star: false
creator: Team TU Munich 2011
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K322123
component: BBa_R0082
component: BBa_K322124
sequence annotation: BBa_K322123
sequence annotation: BBa_K322124
sequence annotation: BBa_R0082
sequence: https://synbiohub.org/public/igem/BBa_K568000_sequence/1

BBa_K322123
type: Component
persistent identity: component2125703
display id: component2125703
version: 1
top level: BBa_K568000
definition: BBa_K322123
access: public

BBa_R0082
type: Component
persistent identity: component2125712
display id: component2125712
version: 1
top level: BBa_K568000
definition: OmpR
access: public

BBa_K322124
type: Component
persistent identity: component2125706
display id: component2125706
version: 1
top level: BBa_K568000
definition: BBa_K322124
access: public

BBa_K322123
type: SequenceAnnotation
persistent identity: annotation2125703
display id: annotation2125703
version: 1
top level: BBa_K568000
location: https://synbiohub.org/public/igem/BBa_K568000/annotation2125703/range2125703/1
component: BBa_K322123

https://synbiohub.org/public/igem/BBa_K568000/annotation2125703/range2125703/1
type: Range
persistent identity: range2125703
display id: range2125703
version: 1
top level: BBa_K568000
direction: direction/reverse
start: 1
end: 1399
orientation: inline

BBa_K322124
type: SequenceAnnotation
persistent identity: annotation2125706
display id: annotation2125706
version: 1
top level: BBa_K568000
location: https://synbiohub.org/public/igem/BBa_K568000/annotation2125706/range2125706/1
component: BBa_K322124

https://synbiohub.org/public/igem/BBa_K568000/annotation2125706/range2125706/1
type: Range
persistent identity: range2125706
display id: range2125706
version: 1
top level: BBa_K568000
direction: direction/reverse
start: 1408
end: 3660
orientation: inline

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2125712
display id: annotation2125712
version: 1
top level: BBa_K568000
location: https://synbiohub.org/public/igem/BBa_K568000/annotation2125712/range2125712/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_K568000/annotation2125712/range2125712/1
type: Range
persistent identity: range2125712
display id: range2125712
version: 1
top level: BBa_K568000
direction: direction/reverse
start: 3669
end: 3776
orientation: inline

OmpR
type: ComponentDefinition
persistent identity: BBa R0082
display id: BBa_R0082
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (OmpR, positive)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: NC_000193 E. coli K12
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1. An alternate version, BBa_R0083, cuts out the C2 and C3 sites. The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown.
star: true
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: C1 OmpR
sequence annotation: C2 OmpR
sequence annotation: C3 OmpR
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0082_sequence/1

C1 OmpR
type: SequenceAnnotation
persistent identity: annotation301154
display id: annotation301154
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
type: Range
persistent identity: range301154
display id: range301154
version: 1
top level: OmpR
direction: direction/forward
start: 13
end: 30
orientation: inline

C2 OmpR
type: SequenceAnnotation
persistent identity: annotation301155
display id: annotation301155
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
type: Range
persistent identity: range301155
display id: range301155
version: 1
top level: OmpR
direction: direction/forward
start: 34
end: 51
orientation: inline

C3 OmpR
type: SequenceAnnotation
persistent identity: annotation301156
display id: annotation301156
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
type: Range
persistent identity: range301156
display id: range301156
version: 1
top level: OmpR
direction: direction/forward
start: 54
end: 71
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301166
display id: annotation301166
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
type: Range
persistent identity: range301166
display id: range301166
version: 1
top level: OmpR
direction: direction/forward
start: 75
end: 80
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301167
display id: annotation301167
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
type: Range
persistent identity: range301167
display id: range301167
version: 1
top level: OmpR
direction: direction/forward
start: 98
end: 103
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_B1006
type: ComponentDefinition
persistent identity: BBa B1006
display id: BBa_B1006
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artificial, large, %T~>90)
created: 2006-08-30T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90%
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
star: true
creator: Haiyao Huang
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: B1006
sequence annotation: PolyA
sequence annotation: modified thr terminator
sequence: https://synbiohub.org/public/igem/BBa_B1006_sequence/1

B1006
type: SequenceAnnotation
persistent identity: annotation1898428
display id: annotation1898428
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
type: Range
persistent identity: range1898428
display id: range1898428
version: 1
top level: BBa_B1006
direction: direction/forward
start: 1
end: 39
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898431
display id: annotation1898431
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
type: Range
persistent identity: range1898431
display id: range1898431
version: 1
top level: BBa_B1006
direction: direction/reverse
start: 1
end: 9
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation1898429
display id: annotation1898429
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
type: Range
persistent identity: range1898429
display id: range1898429
version: 1
top level: BBa_B1006
direction: direction/forward
start: 10
end: 31
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898430
display id: annotation1898430
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
type: Range
persistent identity: range1898430
display id: range1898430
version: 1
top level: BBa_B1006
direction: direction/forward
start: 32
end: 39
orientation: inline

BBa_K322123
type: ComponentDefinition
persistent identity: BBa K322123
display id: BBa_K322123
version: 1
was generated by: iGEM to SBOL conversion
description: Phycobilin synthesis operon without terminator
created: 2010-09-24T11:00:00Z
modified: 2015-05-08T01:11:59Z
mutable provenance: This is derived from K098010 but PCR was used to remove the terminator, so that cph8 could be added at the end of the operon.
owned by: james
owned by: myers
mutable description: Phycobilin is chromophores (light-capturing molecules) found in cyanobacteria and in the chloroplasts of red algae, glaucophytes and some cryptomonads (though not in green algae and higher plants)， used in light sensor.
discontinued: false
dominant: false
experience: experience/None
group u list: _441_
m user id: 0
owner id: 6225
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: The actual forward sequence has the same changes at the start as seen in clone H11 described above. The reverse sequence is as expected. Features same as for the one above apart from the terminator.
star: false
creator: Chris French and Richard Edward Partridge-Hicks
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: J23110
sequence annotation: B0032
sequence annotation: Heme oxygenase
sequence annotation: rbs
sequence annotation: pcyA
sequence: https://synbiohub.org/public/igem/BBa_K322123_sequence/1

J23110
type: SequenceAnnotation
persistent identity: annotation2083043
display id: annotation2083043
version: 1
top level: BBa_K322123
location: https://synbiohub.org/public/igem/BBa_K322123/annotation2083043/range2083043/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K322123/annotation2083043/range2083043/1
type: Range
persistent identity: range2083043
display id: range2083043
version: 1
top level: BBa_K322123
direction: direction/forward
start: 1
end: 35
orientation: inline

B0032
type: SequenceAnnotation
persistent identity: annotation2083044
display id: annotation2083044
version: 1
top level: BBa_K322123
location: https://synbiohub.org/public/igem/BBa_K322123/annotation2083044/range2083044/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K322123/annotation2083044/range2083044/1
type: Range
persistent identity: range2083044
display id: range2083044
version: 1
top level: BBa_K322123
direction: direction/forward
start: 43
end: 54
orientation: inline

Heme oxygenase
type: SequenceAnnotation
persistent identity: annotation2083045
display id: annotation2083045
version: 1
top level: BBa_K322123
location: https://synbiohub.org/public/igem/BBa_K322123/annotation2083045/range2083045/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K322123/annotation2083045/range2083045/1
type: Range
persistent identity: range2083045
display id: range2083045
version: 1
top level: BBa_K322123
direction: direction/forward
start: 63
end: 629
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2083046
display id: annotation2083046
version: 1
top level: BBa_K322123
location: https://synbiohub.org/public/igem/BBa_K322123/annotation2083046/range2083046/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K322123/annotation2083046/range2083046/1
type: Range
persistent identity: range2083046
display id: range2083046
version: 1
top level: BBa_K322123
direction: direction/forward
start: 630
end: 644
orientation: inline

pcyA
type: SequenceAnnotation
persistent identity: annotation2083047
display id: annotation2083047
version: 1
top level: BBa_K322123
location: https://synbiohub.org/public/igem/BBa_K322123/annotation2083047/range2083047/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K322123/annotation2083047/range2083047/1
type: Range
persistent identity: range2083047
display id: range2083047
version: 1
top level: BBa_K322123
direction: direction/forward
start: 650
end: 1396
orientation: inline

BBa_K322124
type: ComponentDefinition
persistent identity: BBa K322124
display id: BBa_K322124
version: 1
was generated by: iGEM to SBOL conversion
description: Red light sensor Cph8 (from Coliroid) with RBS
created: 2010-09-24T11:00:00Z
modified: 2015-05-08T01:11:59Z
mutable provenance: BBa_I15010 (Photosensing Chimaera (Cph8) (not working, but amplified out of the whole construct))
owned by: james
owned by: myers
mutable description: Cph8 is a combination of cph1 and envZ which, together with PCB, form a transmembrane, histidine kinase-based light sensing system. It contains the photoreceptor from Cph1 and the histidine kinase and response-regulator from EnvZ???OmpR, producing a particularly strongresponse to light. Features: cph8 coding sequence 16 to 2250, RBS 6 to 10.
discontinued: false
dominant: false
experience: experience/None
group u list: _441_
m user id: 0
owner id: 6225
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Notes: we were unable to revive BioBrick I15010 with the coding sequence of the cph8 red light sensor fusion protein, so we sought to amplify it from BioBrick BBa_M30109. This led to numerous problems. It is clear from the Registry sequence data that M30109 is not what it is supposed to be. It does include cph8 with a ribosome binding site, but the RBS is not joined to cph8 by a standard scar; promoter R0040 may be present, but there appears to be an EcoRI site a little upstream of the ribosome binding site so that, when we cloned our PCR product, we failed to recover the promoter region; also the PstI site in cph8, which was mutated out in I15010, is still present in the version in M30109. We therefore performed corrective PCR to obtain a clone with the correct prefix, a ribosome binding site, and the cph8 coding sequence with the PstI site properly removed, which we present here, cloned in pSB1C3.
star: false
creator: Chris French and Richard Edward Partridge-Hicks
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: RBS
sequence annotation: Cph8
sequence: https://synbiohub.org/public/igem/BBa_K322124_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2083481
display id: annotation2083481
version: 1
top level: BBa_K322124
location: https://synbiohub.org/public/igem/BBa_K322124/annotation2083481/range2083481/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K322124/annotation2083481/range2083481/1
type: Range
persistent identity: range2083481
display id: range2083481
version: 1
top level: BBa_K322124
direction: direction/forward
start: 6
end: 10
orientation: inline

Cph8
type: SequenceAnnotation
persistent identity: annotation2083482
display id: annotation2083482
version: 1
top level: BBa_K322124
location: https://synbiohub.org/public/igem/BBa_K322124/annotation2083482/range2083482/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K322124/annotation2083482/range2083482/1
type: Range
persistent identity: range2083482
display id: range2083482
version: 1
top level: BBa_K322124
direction: direction/forward
start: 16
end: 2250
orientation: inline

https://synbiohub.org/public/igem/BBa_K568008_sequence/1
type: Sequence
persistent identity: BBa K568008 sequence
display id: BBa_K568008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtattatgctagctactagagtcacacaggaaaggtgcacatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctagttaaagaggagaaaggatccatggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataatactagagaggagaaaggtacccatggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccgcccacctgattcagccccacggtttagtggtggtcctgcaagaaccagacctcaccatcagccaaattagcgccaactgcaccggcattttagggcgatcgccagaggatttgttgggcagaaccctaggggaagtgtttgatagctttcagattgatcccatccagagtcgcctaacggccggacaaatcagcagcctcaaccccagtaaactttgggcgcgggtcatgggggacgactttgtcatttttgacggggtttttcatcgcaacagtgacggtttattggtatgtgaactcgagccagcctacacttccgataatctgcccttcctcggtttttatcacatggccaacgctgccctgaatcggttgcgccaacaagctaatctacgggatttctacgatgttattgtcgaagaagtccgccgtatgactggctttgaccgggtgatgctataccgctttgatgaaaataaccacggtgatgtcattgccgaagataaacggga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K081012_sequence/1
type: Sequence
persistent identity: BBa K081012 sequence
display id: BBa_K081012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K568000_sequence/1
type: Sequence
persistent identity: BBa K568000 sequence
display id: BBa_K568000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtattatgctagctactagagtcacacaggaaaggtgcacatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctagttaaagaggagaaaggatccatggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataatactagagaggagaaaggtacccatggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccgcccacctgattcagccccacggtttagtggtggtcctgcaagaaccagacctcaccatcagccaaattagcgccaactgcaccggcattttagggcgatcgccagaggatttgttgggcagaaccctaggggaagtgtttgatagctttcagattgatcccatccagagtcgcctaacggccggacaaatcagcagcctcaaccccagtaaactttgggcgcgggtcatgggggacgactttgtcatttttgacggggtttttcatcgcaacagtgacggtttattggtatgtgaactcgagccagcctacacttccgataatctgcccttcctcggtttttatcacatggccaacgctgccctgaatcggttgcgccaacaagctaatctacgggatttctacgatgttattgtcgaagaagtccgccgtatgactggctttgaccgggtgatgctataccgctttgatgaaaataaccacggtgatgtcattgccgaagataaacggga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0082_sequence/1
type: Sequence
persistent identity: BBa R0082 sequence
display id: BBa_R0082_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1006_sequence/1
type: Sequence
persistent identity: BBa B1006 sequence
display id: BBa_B1006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaaaaaaaccccgcccctgacagggcggggtttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K322123_sequence/1
type: Sequence
persistent identity: BBa K322123 sequence
display id: BBa_K322123_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtattatgctagctactagagtcacacaggaaaggtgcacatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctagttaaagaggagaaaggatccatggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K322124_sequence/1
type: Sequence
persistent identity: BBa K322124 sequence
display id: BBa_K322124_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aggagaaaggtacccatggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccgcccacctgattcagccccacggtttagtggtggtcctgcaagaaccagacctcaccatcagccaaattagcgccaactgcaccggcattttagggcgatcgccagaggatttgttgggcagaaccctaggggaagtgtttgatagctttcagattgatcccatccagagtcgcctaacggccggacaaatcagcagcctcaaccccagtaaactttgggcgcgggtcatgggggacgactttgtcatttttgacggggtttttcatcgcaacagtgacggtttattggtatgtgaactcgagccagcctacacttccgataatctgcccttcctcggtttttatcacatggccaacgctgccctgaatcggttgcgccaacaagctaatctacgggatttctacgatgttattgtcgaagaagtccgccgtatgactggctttgaccgggtgatgctataccgctttgatgaaaataaccacggtgatgtcattgccgaagataaacgggatgatatggaaccctatttgggcctgcactatcccgaatcggatattccccaacccgcccgtcggctatttatccacaaccccattcgagtaattcccgatgtttatggtgtggcggtgcccctgaccccagcggttaaccccagcaccaaccgagcggtggatttaacagaatccattctgcgcagtgcgtaccattgccacttgacctatctgaaaaatatgggggtaggagcgtctttaaccatttccctaattaaggacggccatctctgggggctcattgcctgccaccatcaaacccccaaagtaattccctttgaactgcgtaaagcctgcgaattttttggtcgggtggtgtttagcaacatttccgcccaggaagatacggaaaccttcgattaccgggtgcagctggcggagcatgaagcggttttattggacaaaatgaccacggcggcggattttgtcgaaggattaactaatcatcccgatcgcctgttgggattaacgggctcccagggggcggccatttgctttggggaaaaattgattttagtaggggaaaccccggacgagaaagcagtgcaatatttactgcaatggttggagaatcgggaagtgcaagacgttttcttcacctcttccctctcacaaatttatcctgatgcagtgaattttaaatccgtggccagtggcttattggccattcccattgcccgtcacaactttttgctctggtttcgccctgaagtgttgcaaacggttaattggggcggtgacccaaatcatgcttacgaagctacccaggaagacggtaaaatcgagctccatccccgccaatcctttgacctctggaaagaaattgtccgactccaatctttgccctggcaatcggtggaaatccaaagtgccctggccctgaaaaaggcgatcgtcaacctcattttgcgccaggcagaagaattgcatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctgggtcaaagtcagcagcggaacggagccgaatcgcgcctg…
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z