LOCUS       BBa_K569001              937 bp    DNA     linear       25-Nov-2024
DEFINITION  PcstA+RBS+LuxI+double terminator
ACCESSION   BBa_K569001
VERSION     BBa_K569001.1  
FEATURES             Location/Qualifiers
     misc_feature    1..42
                     /label=cAMP receptor protein binding site
     misc_feature    101..131
                     /label=RNA polymerase binding site
     promoter        1..131
                     /label=BBa_K118011
     misc_feature    144..147
                     /label=conserved
     RBS             140..151
                     /label=BBa_B0034
     misc_feature    737..768
                     /label=LVA
     CDS             158..736
                     /label=luxI
     misc_feature    158..775
                     /label=BBa_C0061
     misc_feature    776..800
                     /label=Help:Barcodes
     CDS             158..775
                     /label=BBa_C0061
     stem_loop       820..863
                     /label=stem_loop
     misc_feature    809..888
                     /label=BBa_B0010
     terminator      809..888
                     /label=BBa_B0010
     stem_loop       904..923
                     /label=T7 TE
     misc_feature    930..930
                     /label=stop
     polyA_site      924..937
                     /label=polya
     misc_feature    897..937
                     /label=BBa_B0012
     terminator      897..937
                     /label=BBa_B0012
     misc_feature    140..937
                     /label=BBa_K569000
ORIGIN
        1 actcggttaa cggagtgatc gagttaacat tgttaagtta aatattggtt tcaactccga
       61 tttacatggt tgctgtgttg ttaaattgta caaagatgtt atagaaacaa aatgtaacat
      121 ctctatggac atactagaga aagaggagaa atactagatg actataatga taaaaaaatc
      181 ggattttttg gcaattccat cggaggagta taaaggtatt ctaagtcttc gttatcaagt
      241 gtttaagcaa agacttgagt gggacttagt tgtagaaaat aaccttgaat cagatgagta
      301 tgataactca aatgcagaat atatttatgc ttgtgatgat actgaaaatg taagtggatg
      361 ctggcgttta ttacctacaa caggtgatta tatgctgaaa agtgtttttc ctgaattgct
      421 tggtcaacag agtgctccca aagatcctaa tatagtcgaa ttaagtcgtt ttgctgtagg
      481 taaaaatagc tcaaagataa ataactctgc tagtgaaatt acaatgaaac tatttgaagc
      541 tatatataaa cacgctgtta gtcaaggtat tacagaatat gtaacagtaa catcaacagc
      601 aatagagcga tttttaaagc gtattaaagt tccttgtcat cgtattggag acaaagaaat
      661 tcatgtatta ggtgatacta aatcggttgt attgtctatg cctattaatg aacagtttaa
      721 aaaagcagtc ttaaatgctg caaacgacga aaactacgct ttagtagctt aataactctg
      781 atagtgctag tgtagatctc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
      841 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
      901 actggctcac cttcgggtgg gcctttctgc gtttata
//