LOCUS       BBa_K569010             1701 bp    DNA     linear       08-Feb-2026
DEFINITION  ctx+CheZ mutant
ACCESSION   BBa_K569010
VERSION     BBa_K569010.1  
FEATURES             Location/Qualifiers
     promoter        1..145
                     /label=ctx promoter
     misc_feature    145..145
                     /label=transcription start
     misc_feature    133..138
                     /label=-10 region
     promoter        1..145
                     /label=BBa_J07007
     RBS             154..168
                     /label=RBS-1\Strong
     RBS             161..165
                     /label=RBS
     misc_feature    154..168
                     /label=BBa_B0030
     RBS             154..168
                     /label=BBa_B0030
     CDS             175..894
                     /label=GFP protein
     CDS             175..894
                     /label=BBa_E0040
     stem_loop       914..957
                     /label=stem_loop
     misc_feature    903..982
                     /label=BBa_B0010
     terminator      903..982
                     /label=BBa_B0010
     stem_loop       998..1017
                     /label=T7 TE
     misc_feature    1024..1024
                     /label=stop
     polyA_site      1018..1031
                     /label=polya
     misc_feature    991..1031
                     /label=BBa_B0012
     terminator      991..1031
                     /label=BBa_B0012
     misc_feature    1..1031
                     /label=BBa_J07011
     CDS             1038..1701
                     /label=BBa_K569007
ORIGIN
        1 aacagaaaat gataaaaaag gactaatagt atattttgat ttttgatttt tgatttttga
       61 tttttgattt ttgatttttg atttttgatt tcaaataata caaatttatt tacttattta
      121 attgttttga tcaattattt ttctgtacta gagattaaag aggagaaata ctagatgcgt
      181 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
      241 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt
      301 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact
      361 actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac
      421 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat
      481 gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga
      541 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa
      601 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa
      661 gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat
      721 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc
      781 acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag
      841 tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag
      901 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
      961 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1021 ctgcgtttat atactagatg caaccatcaa tcaaacctgc tgacgagcat tcagctgctg
     1081 gcgatatcat tgcgcgcatc ggcagcctga cgcgtatgct gcgcgacagt ttgcgggaac
     1141 tggggctgga tcaggccatt gccgaagcgg cggaagccat ccccgatgcg cgcgatcgtt
     1201 tgtactatgt tgtgcagatg accgcccagg ctgcggagcg ggcgctgaac agtgttgagg
     1261 cgtcacaacc gcatcaggat caaatggaga aatcagcaaa agcgttaacc caacgttggg
     1321 atgactggtt tgccgatccg attgaccttg ccgacgcccg tgaactggta acagatacac
     1381 gacaatttct ggcagatgta cccgcgcata ccagctttac taacgcgcaa ctgctggaaa
     1441 tcatgatggc gcaggatttt caggatctca ccgggcaggt cattaagcgg atgatggatg
     1501 tcattcagga gatcgaacgc cagttgctga tggtgctgtt ggaaaacatc ccggaacagg
     1561 actcgcgtcc aaaacgtgaa aaccagagtt tgcttaatgg acctcaggtc gataccagca
     1621 aagccggtgt ggtagccagt caggatcagg tggacgattt gttggatagt cttggatttt
     1681 gatttgtatt gcctgatgtg g
//