LOCUS       BBa_K569015             2467 bp    DNA     linear       19-Jan-2026
DEFINITION  ctx+ToxR+FGFR
ACCESSION   BBa_K569015
VERSION     BBa_K569015.1  
FEATURES             Location/Qualifiers
     promoter        1..145
                     /label=ctx promoter
     misc_feature    145..145
                     /label=transcription start
     misc_feature    133..138
                     /label=-10 region
     promoter        1..145
                     /label=BBa_J07007
     RBS             154..168
                     /label=RBS-1\Strong
     RBS             161..165
                     /label=RBS
     misc_feature    154..168
                     /label=BBa_B0030
     RBS             154..168
                     /label=BBa_B0030
     CDS             175..894
                     /label=GFP protein
     CDS             175..894
                     /label=BBa_E0040
     stem_loop       914..957
                     /label=stem_loop
     misc_feature    903..982
                     /label=BBa_B0010
     terminator      903..982
                     /label=BBa_B0010
     stem_loop       998..1017
                     /label=T7 TE
     misc_feature    1024..1024
                     /label=stop
     polyA_site      1018..1031
                     /label=polya
     misc_feature    991..1031
                     /label=BBa_B0012
     terminator      991..1031
                     /label=BBa_B0012
     misc_feature    1..1031
                     /label=BBa_J07011
     misc_feature    1038..1583
                     /label=Cytoplasmic Region
     CDS             1038..1667
                     /label=Coding Sequence for ToxR'
     misc_feature    1043..1045
                     /label=Start Codon
     misc_feature    1584..1631
                     /label=Transmembrane Region
     misc_feature    1632..1667
                     /label=Periplasmic Region
     CDS             1038..1667
                     /label=BBa_J07009
     CDS             1676..2467
                     /label=BBa_K569011
     misc_feature    1038..2467
                     /label=BBa_K569012
ORIGIN
        1 aacagaaaat gataaaaaag gactaatagt atattttgat ttttgatttt tgatttttga
       61 tttttgattt ttgatttttg atttttgatt tcaaataata caaatttatt tacttattta
      121 attgttttga tcaattattt ttctgtacta gagattaaag aggagaaata ctagatgcgt
      181 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
      241 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt
      301 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact
      361 actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac
      421 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat
      481 gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga
      541 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa
      601 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa
      661 gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat
      721 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc
      781 acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag
      841 tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag
      901 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
      961 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1021 ctgcgtttat atactagatg ttcggattag gacacaactc aaaagagata tcgatgagtc
     1081 atattggtac taaattcatt cttgctgaaa aatttacctt cgatccccta agcaatactc
     1141 tgattgacaa agaagatagt gaagagatca ttcgattagg cagcaacgaa agccgaatcc
     1201 tttggctgct ggcccaacgt ccaaacgagg taatttctcg caatgatttg catgactttg
     1261 tttggcgaga gcaaggtttt gaagtcgatg attccagctt aacccaagcc atttcgactc
     1321 tgcgcaaaat gctcaaagat tcgacaaagt ccccacaata cgtcaaaacg gttccgaagc
     1381 gcggttacca attgatcgcc cgagtggaaa cggttgaaga agagatggct cgcgaaaacg
     1441 aagctgctca tgacatctct cagccagaat ctgtcaatga atacgcagaa tcaagcagtg
     1501 tgccttcatc agccactgta gtgaacacac cgcagccagc caatgtcgtg gcgaataaat
     1561 cggctccaaa cttggggaat cgactgttta ttctgatagc ggtcttactt cccctcgcag
     1621 tattactgct cactaaccca agccaatcca gctttaaacc cctaacgtac tagagccgtc
     1681 cccgaccttg cctgaacaag atgctctccc ctcctcggag gatgatgatg atgatgatga
     1741 ctcctcttca gaggagaaag aaacagataa caccaaacca aaccccgtag ctccatattg
     1801 gacatcccca gaaaagatgg aaaagaaatt gcatgcagtg ccggctgcca agacagtgaa
     1861 gttcaaatgc ccttccagtg ggaccccaaa ccccacactg cgctggttga aaaatggcaa
     1921 agagttcaaa cctgaccaca gaattggagg ctacaaggtc cgttatgcca cctggagcat
     1981 cataatggac tctgtggtgc cctctgacaa gggcaactac acctgcattg tggagaatga
     2041 gtacggcagc atcaaccaca cataccagct ggatgtcgtg gagcggtccc ctcaccggcc
     2101 catcctgcaa gcagggttgc ccgccaacaa aacagtggcc ctgggtagca acgtggagtt
     2161 catgtgtaag gtgtacagtg acccgcagcc gcacatccag tggctaaagc acatcgaggt
     2221 gaatgggagc aagattggcc cagacaacct gccttatgtc cagatcttga agactgctgg
     2281 agttaatacc accgacaaag agatggaggt gcttcactta agaaatgtct cctttgagga
     2341 cgcaggggag tatacgtgct tggcgggtaa ctctatcgga ctctcccatc actctgcatg
     2401 gttgaccgtt ctggaagccc tggaagagag gccggcagtg atgacctcgc ccctgtacct
     2461 ggagatc
//