BBa_K569015
type: ComponentDefinition
persistent identity: BBa K569015
display id: BBa_K569015
version: 1
was generated by: iGEM to SBOL conversion
description: ctx+ToxR+FGFR
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:44Z
mutable provenance: ?
owned by: james
owned by: myers
mutable description: ?
discontinued: true
dominant: false
experience: experience/None
group u list: _737_
m user id: 0
owner id: 9213
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: ?
star: false
creator: David Golynskiy
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K569012
component: BBa_J07011
sequence annotation: BBa_J07011
sequence annotation: BBa_K569012
sequence: https://synbiohub.org/public/igem/BBa_K569015_sequence/1

BBa_K569012
type: Component
persistent identity: component2144847
display id: component2144847
version: 1
top level: BBa_K569015
definition: BBa_K569012
access: public

BBa_J07011
type: Component
persistent identity: component2144839
display id: component2144839
version: 1
top level: BBa_K569015
definition: BBa_J07011
access: public

BBa_J07011
type: SequenceAnnotation
persistent identity: annotation2144839
display id: annotation2144839
version: 1
top level: BBa_K569015
location: https://synbiohub.org/public/igem/BBa_K569015/annotation2144839/range2144839/1
component: BBa_J07011

https://synbiohub.org/public/igem/BBa_K569015/annotation2144839/range2144839/1
type: Range
persistent identity: range2144839
display id: range2144839
version: 1
top level: BBa_K569015
direction: direction/reverse
start: 1
end: 1031
orientation: inline

BBa_K569012
type: SequenceAnnotation
persistent identity: annotation2144847
display id: annotation2144847
version: 1
top level: BBa_K569015
location: https://synbiohub.org/public/igem/BBa_K569015/annotation2144847/range2144847/1
component: BBa_K569012

https://synbiohub.org/public/igem/BBa_K569015/annotation2144847/range2144847/1
type: Range
persistent identity: range2144847
display id: range2144847
version: 1
top level: BBa_K569015
direction: direction/reverse
start: 1038
end: 2467
orientation: inline

BBa_J07011
type: ComponentDefinition
persistent identity: BBa J07011
display id: BBa_J07011
version: 1
was generated by: iGEM to SBOL conversion
description: ToxR promoter (ctx) controlling GFP
created: 2005-07-06T11:00:00Z
modified: 2015-08-31T04:08:19Z
owned by: james
owned by: myers
mutable description: This part is a reporter construct designed to let us know the activity of the ToxR regulated promoter, ctx.
discontinued: false
dominant: false
experience: experience/None
group u list: _21_
m user id: 0
owner id: 363
owning group id: 21
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: bosworth
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0012
component: BBa_B0010
component: BBa_E0040
component: BBa_B0030
component: BBa_J07007
sequence annotation: BBa_J07007
sequence annotation: BBa_B0030
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J07011_sequence/1

BBa_B0012
type: Component
persistent identity: component1576821
display id: component1576821
version: 1
top level: BBa_J07011
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1576811
display id: component1576811
version: 1
top level: BBa_J07011
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component1576806
display id: component1576806
version: 1
top level: BBa_J07011
definition: GFP
access: public

BBa_B0030
type: Component
persistent identity: component1576803
display id: component1576803
version: 1
top level: BBa_J07011
definition: BBa_B0030
access: public

BBa_J07007
type: Component
persistent identity: component1576795
display id: component1576795
version: 1
top level: BBa_J07011
definition: BBa_J07007
access: public

BBa_J07007
type: SequenceAnnotation
persistent identity: annotation1576795
display id: annotation1576795
version: 1
top level: BBa_J07011
location: https://synbiohub.org/public/igem/BBa_J07011/annotation1576795/range1576795/1
component: BBa_J07007

https://synbiohub.org/public/igem/BBa_J07011/annotation1576795/range1576795/1
type: Range
persistent identity: range1576795
display id: range1576795
version: 1
top level: BBa_J07011
direction: direction/forward
start: 1
end: 145
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1576803
display id: annotation1576803
version: 1
top level: BBa_J07011
location: https://synbiohub.org/public/igem/BBa_J07011/annotation1576803/range1576803/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J07011/annotation1576803/range1576803/1
type: Range
persistent identity: range1576803
display id: range1576803
version: 1
top level: BBa_J07011
direction: direction/forward
start: 154
end: 168
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1576806
display id: annotation1576806
version: 1
top level: BBa_J07011
location: https://synbiohub.org/public/igem/BBa_J07011/annotation1576806/range1576806/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_J07011/annotation1576806/range1576806/1
type: Range
persistent identity: range1576806
display id: range1576806
version: 1
top level: BBa_J07011
direction: direction/forward
start: 175
end: 894
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1576811
display id: annotation1576811
version: 1
top level: BBa_J07011
location: https://synbiohub.org/public/igem/BBa_J07011/annotation1576811/range1576811/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J07011/annotation1576811/range1576811/1
type: Range
persistent identity: range1576811
display id: range1576811
version: 1
top level: BBa_J07011
direction: direction/forward
start: 903
end: 982
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1576821
display id: annotation1576821
version: 1
top level: BBa_J07011
location: https://synbiohub.org/public/igem/BBa_J07011/annotation1576821/range1576821/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J07011/annotation1576821/range1576821/1
type: Range
persistent identity: range1576821
display id: range1576821
version: 1
top level: BBa_J07011
direction: direction/forward
start: 991
end: 1031
orientation: inline

BBa_K569012
type: ComponentDefinition
persistent identity: BBa K569012
display id: BBa_K569012
version: 1
was generated by: iGEM to SBOL conversion
description: ToxR+FGFR
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:44Z
mutable provenance: ?
owned by: james
owned by: myers
mutable description: ?
discontinued: false
dominant: false
experience: experience/None
group u list: _737_
m user id: 0
owner id: 9213
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: ?
star: false
creator: David Golynskiy & Tyler Guinn
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K569011
component: BBa_J07009
sequence annotation: BBa_J07009
sequence annotation: BBa_K569011
sequence: https://synbiohub.org/public/igem/BBa_K569012_sequence/1

BBa_K569011
type: Component
persistent identity: component2144803
display id: component2144803
version: 1
top level: BBa_K569012
definition: BBa_K569011
access: public

BBa_J07009
type: Component
persistent identity: component2144802
display id: component2144802
version: 1
top level: BBa_K569012
definition: BBa_J07009
access: public

BBa_J07009
type: SequenceAnnotation
persistent identity: annotation2144802
display id: annotation2144802
version: 1
top level: BBa_K569012
location: https://synbiohub.org/public/igem/BBa_K569012/annotation2144802/range2144802/1
component: BBa_J07009

https://synbiohub.org/public/igem/BBa_K569012/annotation2144802/range2144802/1
type: Range
persistent identity: range2144802
display id: range2144802
version: 1
top level: BBa_K569012
direction: direction/reverse
start: 1
end: 630
orientation: inline

BBa_K569011
type: SequenceAnnotation
persistent identity: annotation2144803
display id: annotation2144803
version: 1
top level: BBa_K569012
location: https://synbiohub.org/public/igem/BBa_K569012/annotation2144803/range2144803/1
component: BBa_K569011

https://synbiohub.org/public/igem/BBa_K569012/annotation2144803/range2144803/1
type: Range
persistent identity: range2144803
display id: range2144803
version: 1
top level: BBa_K569012
direction: direction/reverse
start: 639
end: 1430
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_J07007
type: ComponentDefinition
persistent identity: BBa J07007
display id: BBa_J07007
version: 1
was generated by: iGEM to SBOL conversion
description: ctx promoter
created: 2005-07-05T11:00:00Z
modified: 2015-08-31T04:08:19Z
mutable provenance: GENBANK M21250
owned by: james
owned by: myers
mutable description: V.cholerae ctx gene promoter region. Transcription takes place In the presence of a ToxR dimer. We don't have the -10 and -35 sites annotated (need to do this).
discontinued: false
dominant: false
experience: experience/None
group u list: _11_12_21_1_
m user id: 0
owner id: 363
owning group id: 21
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: igem_mit_2005
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: ctx promoter
sequence annotation: -10 region
sequence annotation: transcription start
sequence: https://synbiohub.org/public/igem/BBa_J07007_sequence/1

ctx promoter
type: SequenceAnnotation
persistent identity: annotation1571612
display id: annotation1571612
version: 1
top level: BBa_J07007
location: https://synbiohub.org/public/igem/BBa_J07007/annotation1571612/range1571612/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_J07007/annotation1571612/range1571612/1
type: Range
persistent identity: range1571612
display id: range1571612
version: 1
top level: BBa_J07007
direction: direction/forward
start: 1
end: 145
orientation: inline

-10 region
type: SequenceAnnotation
persistent identity: annotation1579234
display id: annotation1579234
version: 1
top level: BBa_J07007
location: https://synbiohub.org/public/igem/BBa_J07007/annotation1579234/range1579234/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J07007/annotation1579234/range1579234/1
type: Range
persistent identity: range1579234
display id: range1579234
version: 1
top level: BBa_J07007
direction: direction/forward
start: 133
end: 138
orientation: inline

transcription start
type: SequenceAnnotation
persistent identity: annotation1577387
display id: annotation1577387
version: 1
top level: BBa_J07007
location: https://synbiohub.org/public/igem/BBa_J07007/annotation1577387/range1577387/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J07007/annotation1577387/range1577387/1
type: Range
persistent identity: range1577387
display id: range1577387
version: 1
top level: BBa_J07007
direction: direction/forward
start: 145
end: 145
orientation: inline

BBa_K569011
type: ComponentDefinition
persistent identity: BBa K569011
display id: BBa_K569011
version: 1
was generated by: iGEM to SBOL conversion
description: FGFR
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:44Z
mutable provenance: ?
owned by: james
owned by: myers
mutable description: receptor
discontinued: false
dominant: false
experience: experience/None
group u list: _737_
m user id: 0
owner id: 9213
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: NA
star: false
creator: David Golynskiy & Tyler Guinn
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K569011_sequence/1

BBa_J07009
type: ComponentDefinition
persistent identity: BBa J07009
display id: BBa_J07009
version: 1
was generated by: iGEM to SBOL conversion
description: toxicity-gene activator from Vibrio cholerae
created: 2005-07-06T11:00:00Z
modified: 2015-08-31T04:08:19Z
owned by: james
owned by: myers
mutable description: shortened BBa_J07008. Typical ToxR-periplasm-region subsitutions are done @ aa 211. explanation of ToxR domains @ http://model.mit.edu/igem/images/d/d7/Kolmar95.pdf ELIMINATE XbaI SITE PLZZZZZ
discontinued: false
dominant: false
experience: experience/None
group u list: _21_
m user id: 0
owner id: 363
owning group id: 21
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: mit_igem_2005
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Cytoplasmic Region
sequence annotation: Coding Sequence for ToxR'
sequence annotation: Start Codon
sequence annotation: Transmembrane Region
sequence annotation: Periplasmic Region
sequence: https://synbiohub.org/public/igem/BBa_J07009_sequence/1

Cytoplasmic Region
type: SequenceAnnotation
persistent identity: annotation1668506
display id: annotation1668506
version: 1
top level: BBa_J07009
location: https://synbiohub.org/public/igem/BBa_J07009/annotation1668506/range1668506/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J07009/annotation1668506/range1668506/1
type: Range
persistent identity: range1668506
display id: range1668506
version: 1
top level: BBa_J07009
direction: direction/forward
start: 1
end: 546
orientation: inline

Coding Sequence for ToxR'
type: SequenceAnnotation
persistent identity: annotation1668507
display id: annotation1668507
version: 1
top level: BBa_J07009
location: https://synbiohub.org/public/igem/BBa_J07009/annotation1668507/range1668507/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J07009/annotation1668507/range1668507/1
type: Range
persistent identity: range1668507
display id: range1668507
version: 1
top level: BBa_J07009
direction: direction/forward
start: 1
end: 630
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation1668508
display id: annotation1668508
version: 1
top level: BBa_J07009
location: https://synbiohub.org/public/igem/BBa_J07009/annotation1668508/range1668508/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_J07009/annotation1668508/range1668508/1
type: Range
persistent identity: range1668508
display id: range1668508
version: 1
top level: BBa_J07009
direction: direction/forward
start: 6
end: 8
orientation: inline

Transmembrane Region
type: SequenceAnnotation
persistent identity: annotation1668509
display id: annotation1668509
version: 1
top level: BBa_J07009
location: https://synbiohub.org/public/igem/BBa_J07009/annotation1668509/range1668509/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J07009/annotation1668509/range1668509/1
type: Range
persistent identity: range1668509
display id: range1668509
version: 1
top level: BBa_J07009
direction: direction/forward
start: 547
end: 594
orientation: inline

Periplasmic Region
type: SequenceAnnotation
persistent identity: annotation1668510
display id: annotation1668510
version: 1
top level: BBa_J07009
location: https://synbiohub.org/public/igem/BBa_J07009/annotation1668510/range1668510/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J07009/annotation1668510/range1668510/1
type: Range
persistent identity: range1668510
display id: range1668510
version: 1
top level: BBa_J07009
direction: direction/forward
start: 595
end: 630
orientation: inline

https://synbiohub.org/public/igem/BBa_K569015_sequence/1
type: Sequence
persistent identity: BBa K569015 sequence
display id: BBa_K569015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacagaaaatgataaaaaaggactaatagtatattttgatttttgatttttgatttttgatttttgatttttgatttttgatttttgatttcaaataatacaaatttatttacttatttaattgttttgatcaattatttttctgtactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagatgttcggattaggacacaactcaaaagagatatcgatgagtcatattggtactaaattcattcttgctgaaaaatttaccttcgatcccctaagcaatactctgattgacaaagaagatagtgaagagatcattcgattaggcagcaacgaaagccgaatcctttggctgctggcccaacgtccaaacgaggtaatttctcgcaatgatttgcatgactttgtttggcgagagcaaggttttgaagtcgatgattccagcttaacccaagccatttcgactctgcgcaaaatgctcaaagattcgacaaagtccccacaatacgtcaaaacggttccgaagcgcggttaccaattgatcgcccgagtggaaacggttgaagaagagatggctcgcgaaaacgaagctgctcatgacatctctcagccagaatctgtcaatgaatacgcagaatcaagcagtgtgccttcatcagccactgtagtgaacacaccgcagccagccaatgtcgtggcgaataaatcggctccaaacttggggaatcgactgtttattctgatagcggtcttacttcccctcgcagtattactgctcactaacccaagccaatccagctttaaacccctaacgtactagagccgtccccgaccttgcctgaacaagatgctctcccctcctcggaggatgatgatgatgatgatgactcctcttcagaggagaaagaaacagataacaccaaaccaaaccccgtagctccatattggacatccccagaaaagatggaaaagaaattgcatgcagtgccggctgccaagacagtgaagttcaaatgcccttccagtgggaccccaaaccccacactgcgctggttgaaaaatggcaaagagttcaaacctgaccacagaattggaggctacaaggtccgttatgccacctggagcatcataatggactctgtggtgc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J07011_sequence/1
type: Sequence
persistent identity: BBa J07011 sequence
display id: BBa_J07011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacagaaaatgataaaaaaggactaatagtatattttgatttttgatttttgatttttgatttttgatttttgatttttgatttttgatttcaaataatacaaatttatttacttatttaattgttttgatcaattatttttctgtactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K569012_sequence/1
type: Sequence
persistent identity: BBa K569012 sequence
display id: BBa_K569012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgttcggattaggacacaactcaaaagagatatcgatgagtcatattggtactaaattcattcttgctgaaaaatttaccttcgatcccctaagcaatactctgattgacaaagaagatagtgaagagatcattcgattaggcagcaacgaaagccgaatcctttggctgctggcccaacgtccaaacgaggtaatttctcgcaatgatttgcatgactttgtttggcgagagcaaggttttgaagtcgatgattccagcttaacccaagccatttcgactctgcgcaaaatgctcaaagattcgacaaagtccccacaatacgtcaaaacggttccgaagcgcggttaccaattgatcgcccgagtggaaacggttgaagaagagatggctcgcgaaaacgaagctgctcatgacatctctcagccagaatctgtcaatgaatacgcagaatcaagcagtgtgccttcatcagccactgtagtgaacacaccgcagccagccaatgtcgtggcgaataaatcggctccaaacttggggaatcgactgtttattctgatagcggtcttacttcccctcgcagtattactgctcactaacccaagccaatccagctttaaacccctaacgtactagagccgtccccgaccttgcctgaacaagatgctctcccctcctcggaggatgatgatgatgatgatgactcctcttcagaggagaaagaaacagataacaccaaaccaaaccccgtagctccatattggacatccccagaaaagatggaaaagaaattgcatgcagtgccggctgccaagacagtgaagttcaaatgcccttccagtgggaccccaaaccccacactgcgctggttgaaaaatggcaaagagttcaaacctgaccacagaattggaggctacaaggtccgttatgccacctggagcatcataatggactctgtggtgccctctgacaagggcaactacacctgcattgtggagaatgagtacggcagcatcaaccacacataccagctggatgtcgtggagcggtcccctcaccggcccatcctgcaagcagggttgcccgccaacaaaacagtggccctgggtagcaacgtggagttcatgtgtaaggtgtacagtgacccgcagccgcacatccagtggctaaagcacatcgaggtgaatgggagcaagattggcccagacaacctgccttatgtccagatcttgaagactgctggagttaataccaccgacaaagagatggaggtgcttcacttaagaaatgtctcctttgaggacgcaggggagtatacgtgcttggcgggtaactctatcggactctcccatcactctgcatggttgaccgttctggaagccctggaagagaggccggcagtgatgacctcgcccctgtacctggagatc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J07007_sequence/1
type: Sequence
persistent identity: BBa J07007 sequence
display id: BBa_J07007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacagaaaatgataaaaaaggactaatagtatattttgatttttgatttttgatttttgatttttgatttttgatttttgatttttgatttcaaataatacaaatttatttacttatttaattgttttgatcaattatttttctg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K569011_sequence/1
type: Sequence
persistent identity: BBa K569011 sequence
display id: BBa_K569011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccgtccccgaccttgcctgaacaagatgctctcccctcctcggaggatgatgatgatgatgatgactcctcttcagaggagaaagaaacagataacaccaaaccaaaccccgtagctccatattggacatccccagaaaagatggaaaagaaattgcatgcagtgccggctgccaagacagtgaagttcaaatgcccttccagtgggaccccaaaccccacactgcgctggttgaaaaatggcaaagagttcaaacctgaccacagaattggaggctacaaggtccgttatgccacctggagcatcataatggactctgtggtgccctctgacaagggcaactacacctgcattgtggagaatgagtacggcagcatcaaccacacataccagctggatgtcgtggagcggtcccctcaccggcccatcctgcaagcagggttgcccgccaacaaaacagtggccctgggtagcaacgtggagttcatgtgtaaggtgtacagtgacccgcagccgcacatccagtggctaaagcacatcgaggtgaatgggagcaagattggcccagacaacctgccttatgtccagatcttgaagactgctggagttaataccaccgacaaagagatggaggtgcttcacttaagaaatgtctcctttgaggacgcaggggagtatacgtgcttggcgggtaactctatcggactctcccatcactctgcatggttgaccgttctggaagccctggaagagaggccggcagtgatgacctcgcccctgtacctggagatc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J07009_sequence/1
type: Sequence
persistent identity: BBa J07009 sequence
display id: BBa_J07009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgttcggattaggacacaactcaaaagagatatcgatgagtcatattggtactaaattcattcttgctgaaaaatttaccttcgatcccctaagcaatactctgattgacaaagaagatagtgaagagatcattcgattaggcagcaacgaaagccgaatcctttggctgctggcccaacgtccaaacgaggtaatttctcgcaatgatttgcatgactttgtttggcgagagcaaggttttgaagtcgatgattccagcttaacccaagccatttcgactctgcgcaaaatgctcaaagattcgacaaagtccccacaatacgtcaaaacggttccgaagcgcggttaccaattgatcgcccgagtggaaacggttgaagaagagatggctcgcgaaaacgaagctgctcatgacatctctcagccagaatctgtcaatgaatacgcagaatcaagcagtgtgccttcatcagccactgtagtgaacacaccgcagccagccaatgtcgtggcgaataaatcggctccaaacttggggaatcgactgtttattctgatagcggtcttacttcccctcgcagtattactgctcactaacccaagccaatccagctttaaacccctaacg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z