BBa_K571000 1 BBa_K571000 acsAB/pSB1C3 2011-10-01T11:00:00Z 2015-05-08T01:12:44Z *organism: Gluconacetobacter hansenii ATCC 23769 *GenBank: AB091060.1 The acs operon in Gluconacetobacter hansenii is the mechanism that synthesize cellulose synthase, acsAB is one of the gene sequence. The acsAB will produce protein with 2 subunits; one is catalytic subunit which is able to transform UDP-glucose to UDP, another one would be the regulatory subunit of the cellulose synthase which will bind with cyclic di-GMP and activated. From the primer we design, the acsAB sequence is amplified from PCR from the genomic DNA of Gluconacetobacter hansenii, XbaI and SpeI is also added at the both end of primer as the cutting site, so that acsAB and pSB1C3 could easily bind to it. false false _739_ 0 10422 9 It's complicated false By comparative, acsAB is long base pairs gene, so we need more primer during PCR. It is also advisable to separate PCR into a few times to get the most satisfied results. false Lu-Chu Ke annotation2149953 1 acsAB range2149953 1 1 4653 BBa_K571000_sequence 1 atgccagaggttcggtcgtcaacgcagtcagagtcaggaatgtcacagtggatggggaaaattctctccattcgcggtgctgggctgattattggtgtttttggcctgtgtgcgctgattgcggctacgtccgtgaccctgccgccagaacagcagttgattgtggcatttgtatgtgtcgtgatcttttttattgtcggtcataagcccagccgtcggtcccagattttccttgaagtgctgtcagggctggtttcgctgcgctatctgacatggcgcctgacggaaacgctttcattcgatacatggttgcagggtctgcttgggacaatgcttctggtggcggaactttacgccctgatgatgctgttcctcagctacttccagacgatcgcgccgctgcatcgtgcgcctctgccgctgccgccgaaccctgacgaatggcccacggtcgatatcttcgtcccgacctataacgaagaactgagcattgtccgcctgacggtgctgggatcactggggattgactggccaccggaaaaggtgcgggttcatatccttgatgacggccgtcgtcctgaattcgccgcctttgccgctgaatgtggcgcgaattatatcgcccgcccgacgaacgaacatgcaaaggccggtaatcttaactatgccattggtcataccgatggtgattacatcctgatctttgactgcgaccacgtcccgacccgcgccttcctgcagttgacaatgggctggatggtcgaagacccgaagatcgcgctgatgcagaccccgcatcacttctattcccccgacccgttccagcggaacctgtcggctggttatcgtaccccgcccgagggcaacctgttttatggcgtggtgcaggatggcaacgatttctgggatgcgaccttcttttgcgggtcatgtgcaatcctgcgtcgcacggcgattgagcagatcggcggctttgcgacccagaccgtgaccgaagacgcgcataccgcactcaagatgcagcgtctgggctggtccacggcctatctgcgtatcccgcttgccggtggtctcgcgacggaacgcctgatcctgcatatcggacagcgcgtgcgctgggcgcgtgggatgctgcagatcttccgcatcgacaatcctctgttcgggcgtggcctgtcatgggggcagcggctttgttacctgtcggccatgacgtcgttcctgttcgctgtcccgcgcgtcatcttcctgagctccccgctggcgttcctgttctttgggcagaacatcattgccgcgtcgccgctcgcgctgctggcctatgccatcccgcatatgttccacgccgtcggcacggcgtcgaagatcaacaagggctggcgctactccttctggagtgaggtctatgaaaccaccatggcgctgttcctggtgcgcgtgacgattgtcaccctgctcagcccttcacgtgggaagttcaacgtgacggacaagggcgggttgcttgaaaaaggttatttcgaccttggcgccgtctatccgaacatcatccttggcctgatcatgttcggcggtctggcgcgtggtgtctatgaactgtctttcggccatctcgaccagatcgccgaacgtgcctacctgctgaactccgcttgggcgatgctcagcctcatcatcatccttgcggccatcgccgtggggcgtgaaacacagcagaaacgcaacagtcatcgcatccccgcaaccatcccggtggaagtggcgaatgccgatgggtccatcatcgtgacgggcgtgaccgaggacctgtccatgggtggggccgcggtaaagatgtcatggcctgcgaagctgtcggggccgacgccggtttatatccgtactgtccttgatggagaggaactgatcctgcccgccaggatcatccgtgctggcaacgggcgggggatcttcatctggacgattgataacctgcagcaggaattctcggttatccgcctggtgttcggccgtgccgacgcatgggttgactggggcaattacaaggccgaccgcccgctgctcagcctcatggacatggttctcagcgtcaagggcctgttccgttcaagcggcgacatcgtccatcgcagttccccaaccaagccttcggctggcaatgccctgtctgacgatacgaacaacccgtcacgcaaggagcgtgtgctgaagggaaccgtgaaaatggtttcgcttctggcgctgctgacatttgcttcctcggcacaggcggcgtcagcgcccagggccgtcgcggcgaaggccccggcccatcagcccgaagcctctgacctgccgccgttgcccgcgctcctgccggccaccagcggtgcggcgcaggcgggttcgggcgatgccggcgccgatggacccggcagccccacgggccagcccctggcggccgacagtgccgatgcgctggtggaaaatgcggaaaatacgtccgatacggcgactgtccataattataccctcaaggatcttggcgccgcagggtccatcacaatgcgtggccttgccccgttgcaggggatcgagttcgggattccctctgaccagcttgtgacgtccgcgcgccttgtgctgtcgggttcgatgtcgcccaacctgcgtccggaaaccaattcggtcacaatgacgctgaacgagcagtatatcggcacgttgcgtcccgacccggcgcacccgacattcggccccatgtcgttcgagatcaacccgatcttcttcgtcagcggaaaccgtctgaacttcaacttcgcctccgggtcgaagggatgttcggacatcacgaacgatacgctgtgggccacgatctcgcagaactcgcagcttcagatcacgacaatcgcgctgccgccgcgtcgcctgctgtcacgtctgccgcagcccttctatgacaagaacgtgcgccagcatgttacggtcccgatggtgctggcgcagacctatgacccgcagatactcaagtccgcggggattctcgcttcgtggtttggcaagcagacggacttcctcggcgtgacgttcccggtgtcgtccaccatcccacagagtggcaacgccatcctgatcggcgtggccgatgaactgccgaccagcttcgggcggccgcaggtcaatggcccggcggttctggaactgccgaacccgtcggatgcaaatgccacgatcctggtggtgacggggcgtgaccgtgatgaggttattaccgcgagcaagggcatcgccttcgcgtctgctcccctgccgaccgacagccatatggatgtcgcgccggtcgatatcgccccgcgcaagcccaatgacgcgccatcttttatcgcgatggaccatccggtgcgctttggcgaccttgtaacggcaagcaagctacagggaaccggctttacgtccggtgtgctgtcggttccgttccgcattccgcccgacctttatacgtggcgtaaccgcccgtacaagatgcaggtacgtttccgttcccccgcgggggaggcgaaggatgtcgagaagtcacgtctcgatgtcgggatcaacgaggtttacctgcattcctatccgctgcgcgaaacgcatggcctgattggcgcggttttgcagggtgtcggccttgcccgccctgcaagcggcatgcaggtgcatgatctcgacgtaccgccgtggaccgtgttcgggcaggatcagttgaacttctactttgacgcgatgccccttgcgcgcggaatctgtcaaagtggcgcggcaaacaatgcattccatcttgggcttgacccggattccaccattgatttttcccgtgcccatcacatcgcccagatgcccaaccttgcctatatggcgacggtcggttttcctttcaccacctatgccgatctgtcgcagacggcggttgttctgcctgaacacccgaatgccgcgactgttggcgcctatcttgacctgatggggttcatgggcgcggcgacgtggtatccggttgcgggcgtggacattgtgtcggccgatcatgtcagtgacgttgcggaccgtaacctgctggtgatttcgacgctggcgacaagtggcgagatcgcgccgctgctgtcacgttcatcctacgaagtggcggatggtcatttgcgcacggtgtcgcatgcgtccgcgctggataacgcgatcaaggcggtcgacgatccgctgacggctttccgcgaccgtgacagcaagccgcaggatgtcgatacccccctgacgggtggtgtcggcgcgatgatcgaggcggaatcccccctgacggcgggccgcaccgttctggcgctgctgtcgtctgacggtgcggggctgaacaacctccttcagatgttgggggagcgcaagaagcaggcgaatatccagggggacctggttgttgcccatggcgaggacctgtcttcgtaccgcacttcgccggtctatacgatcggcaccctgccgctctggctgtggccggactggtatatgcacaacagaccggttcgtgtgctgcttgtcggcctgttgggatgtattctgatcgtcagtgttcttgcacgggctctggcgcggcatgcggcccggcgtttcaagcagcttgaggatgagaggcgcaagtcgtga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z