BBa_K571002
1
BBa_K571002
CMCax+Ccp/pSB1C3
2011-10-02T11:00:00Z
2015-05-08T01:12:44Z
*organism: Gluconacetobacter hansenii ATCC 23769
*GenBank: EFG85213.1, AAA16970.1
The biobrick contains the cellulose synthesis related genes situated at the upstream and downstream of the cellulose synthase operon, which have been proposed to increase the production of bacteria cellulose, altough their function has not been completely elucidated. This biobrick contains the cmcax and ccp genes in 2,092 bp containing SpeI and PstI restriction sites cloned in pSB1C3. The cmcax (endo-1,4-β-glucanase) is required to enhance the cellulose biosynthesis by providing carbon source to acs operon whereas the ccp encoding the cellulose complementing protein.
false
false
_739_
0
10422
9
It's complicated
false
The cmcax gene has 2 Pst I cutting site while ccp gene has a EcoRI cutting site. During ligation, Spe and AlwN I is used to do enzyme digestion to get the cmcax sequence (approx. 1kb), followed by the binding of pSB1C3. Next, AlwNI and PstI will be used to do the second enzyme digestion in order to get ccp sequence (approx. 1kb), followed by the binding of pSB1C3. It is necessary to avoid restriction of wanted DNA while intentionally cutting the ends of DNA.
false
Lu-Chu Ke
annotation2150404
1
CMCax
range2150404
1
1
1029
annotation2150405
1
Ccp
range2150405
1
1030
2092
BBa_K571002_sequence
1
atgtcggtcatggcggcgatgggaggggcgcaggtgctttcatccaccggtgcgttcgcagacaccgcccccgatgcggtcgcgcagcaatgggccatcttccgcgccaagtatcttcgtcccagcggacgtgtcgtggatacgggcaatggtggcgaatcccatagtgaggggcagggctatggcatgctctttgccgcgtcggcgggggaccttgcgtcgttccagtcgatgtggatgtgggcgcgcaccaacctgcagcataccaatgacaagctgttttcctggcggttcctcaaggggcatcagcccccggtgcccgacaagaacaatgccacagatggcgacctgctgatcgcgcttgcgcttggtcgtgcgggcaagcgtttccagcgccccgattacattcaggacgccatggccatttatggcgatgtgctgaacctgatgacgatgaaggcgggaccgtatgtcgtcctcatgcccggtgctgtcggctttaccaagaaggacagcgtgatcctcaacctgtcctattacgtcatgccctcgctgctgcaggcgttcgaccttacggccgacccgcgctggcgtcaggtgatggaagacgggattcgccttgtttccgccggccgtttcgggcagtggcgcctgccccccgactggctggcggtgaatcgcgccaccggtgcgctgtcgatcgcatcgggatggccgccgcgcttttcctatgatgcgattcgggtgccgctttatttttattgggcgcatatgctggcgccgaacgtgttggctgatttcacccgattctggaataatttcggggctaatgccctgccaggatgggttgatctgacaacaggggcgcgttcgccgtacaacgccccgcctggatatcttgctgttgccgaatgcacggggcttgattctgccggggaactcccgacactggatcatgcgcccgattattattccgcagcgttgacgctgctcgtttacatcgcgcgggcggaggagactataaagtgagtgcttcagggtctgatgaggtggctgggggagggcaggctggaagtccgcaggattttcagcgggtcctgcgttcttttggtgtcgaaggtgggcagtattcctaccggccgtttgttgaccgttcctttgatgtgacaggcgtgcccgaggctgttgaaaggcacttcgatcaggcggagcatgacacggcggttgaggagcaggtcactcccgcgccacaaatcgcggtcgcaccgccaccgccgccagtcgttcctgacccgcccgccatcgtgacggaaaccgcgcccccgccgcctgtcgtggtcagcgctccggtcacgtatgaacccccggctgccgccgtgccggcagagcctcccgttcaggaagcccccgtgcaggcggcgccggttccccccgcgcctgtgcccccgattgcggagcaggctcctcccgcggcgccggacccggcatccgtgccgtatgcgaacgtcgcggcagcacccgttccacctgatcccgcaccggttacgcctgcgccgcaggcgcgcgtgacggggccgaacacccgtatggtggagcccttttcccgcccgcaggtccgcacggtgcaggagggggcaaccccgtcacgtgtaccttcgcgttcaatgaacgctttcccccgcacatcagcatcgtccataagtgagcgtccggtggacaggggtgttgccgatgaatggagtcctgttccgaaggcacgcctcagcccgcgggagcgtccgcgtcccggcgatctgagctttttctttcaggggatgcgcgacacccgtgatgaaaagaagttctttcccgtggcgtccacgcgatcagttcgttctaatgtttccaggatgaccagcatgaccaagacagacacgaattcctctcaggcttctcgtcccggcagccccgtcgcctcgcctgatgggtcgcccacaatggccgaagtgttcatgacgctgggtggtcgtgcgacggaactcctcagcccccgtccttcgctgcgggaggcgctgttgcgtcgtcgtgaaaacgaagaagaatcctaaggccc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z